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The map label for this gene is ubiH [H]

Identifier: 209695956

GI number: 209695956

Start: 2719349

End: 2720548

Strand: Reverse

Name: ubiH [H]

Synonym: VSAL_I2538

Alternate gene names: 209695956

Gene position: 2720548-2719349 (Counterclockwise)

Preceding gene: 209695957

Following gene: 209695955

Centisome position: 81.82

GC content: 40.25

Gene sequence:

>1200_bases
ATGAGTGAAGATATGACACATTATGATGTTGTTATTGTTGGTGGAGCAATGGCTGGAGCTACTCTAGCGTTGGCACTACA
ACAAATGACCAAAGGTGCATTATCTATTGCCGTTATAGAGGCTTTTGAACCCAATATATATAAGCATCCTGGGTATGATT
CTCGTAGTATTGCACTGTCGAGCGGAACCACTCAATTATTGAAAGATGTGTCGTTATGGTCTGAAATAGAACCAGTAGCG
ACGCCTATTTATCATATTCATGTGTCAGATCGTCATCATGCAGGGATAACAGAGCTTACTTCTGCAGAGCTTAATATTGA
CAGCTTAGGTTACGTTGTTGAGTTAGCCGACGTTGGCCGTTTTTATCATCAAAAACTGTTAGCGACCGATAACATTAATT
TTATTTGTCCAGTCACTGTGGTTAATATTGAACGTTTATTAGCGTCATCGATAATTCACTTATCAAACGGAGATGCTATT
TCAACGTCATTAGTGGTTGCGGCTGATGGTGCAGAGTCTCAAACGTGTAAGAGTATGCAGATTGAAGCTCAAGAGTATAA
TTTTGAGCAAGTAGCCATTATTGCGAATGTAACAACAGAGTGCCACCCACAAGGTAAGGCATTTGAACGTTTTACTGAGA
ATGGGCCACTCGCCTTACTTCCAATGTCTCAAGGGCGTAGTTCACTGGTATGGTGTGTAAGCCCAGAAGAAGCAGAACGT
ATTTCGTCTTTTGATGATAAGGCTTTTTTAGCACACTTACAGATCGCGTTTGGCTGGCGTTTAGGGGTAATGAAAAAAGC
AGGAAAACGAGCGCATTATCCATTGTGGTTACGTCATCAAACTCAGCACGTGTCACACCGTTTTGCGGCTATTGGTAATG
CAGCTCAAACATTGCATCCTATTGCAGGGCAAGGGTTTAATTTGGGTATTCGAGATGTTTTTTCGTTGGCTGAATGTATT
TCTGATGCTTTTAGAGATAATGTTGATGTCGGTGATTATTCTGTTCTTTCTCGTTATCAGCAACGACGTCAATCAGATCA
AAAACAAACCATAACAATGACAGCGGGTTTAATCTCTATTTTTGCGAATAATGTTTTTCCTATGGTAGTAGGAAGAAATA
TGAGCTTATACGCGGTTGATATTTTCCGTTCTTTTTTAAAACCCCTAACATTACGTGCAATGGGTCGAGTGAATAGATAA

Upstream 100 bases:

>100_bases
TATTTGAACAAGTGTTAGAACATGTGCATGTTTGTGTATTAACTATTCACTTAGAATTGGGTCAACGTATTCATAAAGAT
GCGTCTAAAGCTGTCCACTA

Downstream 100 bases:

>100_bases
TTATGATGCAAAGTGTAGATATTGCGATTATTGGGGGGGGCATGGTTGGCTTGACTGTTGCTGCTGCTCTAGAAAAAAGC
GGTTTAAGAATCGCAATTAT

Product: 2-octaprenyl-6-methoxyphenyl hydroxylase

Products: H2O; 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSEDMTHYDVVIVGGAMAGATLALALQQMTKGALSIAVIEAFEPNIYKHPGYDSRSIALSSGTTQLLKDVSLWSEIEPVA
TPIYHIHVSDRHHAGITELTSAELNIDSLGYVVELADVGRFYHQKLLATDNINFICPVTVVNIERLLASSIIHLSNGDAI
STSLVVAADGAESQTCKSMQIEAQEYNFEQVAIIANVTTECHPQGKAFERFTENGPLALLPMSQGRSSLVWCVSPEEAER
ISSFDDKAFLAHLQIAFGWRLGVMKKAGKRAHYPLWLRHQTQHVSHRFAAIGNAAQTLHPIAGQGFNLGIRDVFSLAECI
SDAFRDNVDVGDYSVLSRYQQRRQSDQKQTITMTAGLISIFANNVFPMVVGRNMSLYAVDIFRSFLKPLTLRAMGRVNR

Sequences:

>Translated_399_residues
MSEDMTHYDVVIVGGAMAGATLALALQQMTKGALSIAVIEAFEPNIYKHPGYDSRSIALSSGTTQLLKDVSLWSEIEPVA
TPIYHIHVSDRHHAGITELTSAELNIDSLGYVVELADVGRFYHQKLLATDNINFICPVTVVNIERLLASSIIHLSNGDAI
STSLVVAADGAESQTCKSMQIEAQEYNFEQVAIIANVTTECHPQGKAFERFTENGPLALLPMSQGRSSLVWCVSPEEAER
ISSFDDKAFLAHLQIAFGWRLGVMKKAGKRAHYPLWLRHQTQHVSHRFAAIGNAAQTLHPIAGQGFNLGIRDVFSLAECI
SDAFRDNVDVGDYSVLSRYQQRRQSDQKQTITMTAGLISIFANNVFPMVVGRNMSLYAVDIFRSFLKPLTLRAMGRVNR
>Mature_398_residues
SEDMTHYDVVIVGGAMAGATLALALQQMTKGALSIAVIEAFEPNIYKHPGYDSRSIALSSGTTQLLKDVSLWSEIEPVAT
PIYHIHVSDRHHAGITELTSAELNIDSLGYVVELADVGRFYHQKLLATDNINFICPVTVVNIERLLASSIIHLSNGDAIS
TSLVVAADGAESQTCKSMQIEAQEYNFEQVAIIANVTTECHPQGKAFERFTENGPLALLPMSQGRSSLVWCVSPEEAERI
SSFDDKAFLAHLQIAFGWRLGVMKKAGKRAHYPLWLRHQTQHVSHRFAAIGNAAQTLHPIAGQGFNLGIRDVFSLAECIS
DAFRDNVDVGDYSVLSRYQQRRQSDQKQTITMTAGLISIFANNVFPMVVGRNMSLYAVDIFRSFLKPLTLRAMGRVNR

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=378, Percent_Identity=26.984126984127, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI32967307, Length=324, Percent_Identity=28.3950617283951, Blast_Score=128, Evalue=8e-30,
Organism=Escherichia coli, GI1789274, Length=390, Percent_Identity=47.4358974358974, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1786883, Length=378, Percent_Identity=31.2169312169312, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789273, Length=395, Percent_Identity=29.873417721519, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI25146631, Length=423, Percent_Identity=26.7139479905437, Blast_Score=138, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6321694, Length=439, Percent_Identity=23.2346241457859, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19920760, Length=418, Percent_Identity=24.6411483253589, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 43981; Mature: 43850

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDMTHYDVVIVGGAMAGATLALALQQMTKGALSIAVIEAFEPNIYKHPGYDSRSIALS
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEEEEC
SGTTQLLKDVSLWSEIEPVATPIYHIHVSDRHHAGITELTSAELNIDSLGYVVELADVGR
CCHHHHHHHHHHHHHCCHHCCCEEEEEECCCCCCCCHHHCCCCCCCCCCHHEEEHHHHHH
FYHQKLLATDNINFICPVTVVNIERLLASSIIHLSNGDAISTSLVVAADGAESQTCKSMQ
HHHHHHHCCCCCCEEECCHHHHHHHHHHHHHEEECCCCEEEEEEEEEECCCCCCCHHHHE
IEAQEYNFEQVAIIANVTTECHPQGKAFERFTENGPLALLPMSQGRSSLVWCVSPEEAER
EEHHHCCCEEEEEEEECCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCEEEEECCHHHHH
ISSFDDKAFLAHLQIAFGWRLGVMKKAGKRAHYPLWLRHQTQHVSHRFAAIGNAAQTLHP
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHCCHHHHHCC
IAGQGFNLGIRDVFSLAECISDAFRDNVDVGDYSVLSRYQQRRQSDQKQTITMTAGLISI
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHEEEHHHHHHHH
FANNVFPMVVGRNMSLYAVDIFRSFLKPLTLRAMGRVNR
HHCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SEDMTHYDVVIVGGAMAGATLALALQQMTKGALSIAVIEAFEPNIYKHPGYDSRSIALS
CCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEEEEC
SGTTQLLKDVSLWSEIEPVATPIYHIHVSDRHHAGITELTSAELNIDSLGYVVELADVGR
CCHHHHHHHHHHHHHCCHHCCCEEEEEECCCCCCCCHHHCCCCCCCCCCHHEEEHHHHHH
FYHQKLLATDNINFICPVTVVNIERLLASSIIHLSNGDAISTSLVVAADGAESQTCKSMQ
HHHHHHHCCCCCCEEECCHHHHHHHHHHHHHEEECCCCEEEEEEEEEECCCCCCCHHHHE
IEAQEYNFEQVAIIANVTTECHPQGKAFERFTENGPLALLPMSQGRSSLVWCVSPEEAER
EEHHHCCCEEEEEEEECCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCEEEEECCHHHHH
ISSFDDKAFLAHLQIAFGWRLGVMKKAGKRAHYPLWLRHQTQHVSHRFAAIGNAAQTLHP
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHCCHHHHHCC
IAGQGFNLGIRDVFSLAECISDAFRDNVDVGDYSVLSRYQQRRQSDQKQTITMTAGLISI
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHEEEHHHHHHHH
FANNVFPMVVGRNMSLYAVDIFRSFLKPLTLRAMGRVNR
HHCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-octaprenyl-6-methoxyphenol; O2 [C]

Specific reaction: 2-octaprenyl-6-methoxyphenol + O2 = H2O + 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]