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The map label for this gene is ackA [H]

Identifier: 209695556

GI number: 209695556

Start: 2261339

End: 2262535

Strand: Direct

Name: ackA [H]

Synonym: VSAL_I2112

Alternate gene names: 209695556

Gene position: 2261339-2262535 (Clockwise)

Preceding gene: 209695554

Following gene: 209695557

Centisome position: 68.01

GC content: 44.19

Gene sequence:

>1197_bases
ATGTCTAAGCTGGTTTTAGTTTTAAACTGCGGTAGTTCTTCTCTTAAATTTGCGGTTATTGACGCAGAATCTGGTGCAGA
GCACTTAACTGGTCTTGCTGAGTGTCTTGGTCTTCCTGAAGCGCGTATTAAGTGGAAACTTGATGGCAAGCACGAAGCTC
AACTAGGTGAAGGTGCTGCTCACGAAGAAGCTCTATCGTTTATGGTTGAATCTATTCTTGCTTCTAAGCCAGAGCTTAAA
GCTAACCTAGGCGCAATCGGTCACCGTATCGTACACGGTGGTGAGCAATTCACTTCATCTGCTCTTATCACTGACGAAGT
GCTTAAAGGTATTCAAGACGCTGCAACTTTTGCACCTCTTCATAACCCTGCTCACATCATTGGTATCGAAGCGGCTAAAC
ACAACTTCCCTGAACTTCAAAACGTTGCTGTGTTTGATACTGCATTCCACCAAACAATGCCTGAAGAGTCTTACCTATAC
GCTCTACCATACAACCTATACACAGATCACGGTATTCGTCGTTACGGTATGCACGGTACTTCTCACCTGTTCATCACTCG
TGAAGTTGCTGGCCTATTAAACAAACCAGTTGAAGAAGTAAACATCATCAACTGTCACCTAGGTAACGGCGCATCTGTAT
GTGCAATCAAGAACGGTCAATCTGTAGATACTTCTATGGGTCTTACTCCTCTTGAAGGTCTAGTAATGGGTACTCGTTGT
GGTGATATCGATCCTGCGATCATTTTCCACCTACATGACGCACTAGGTTACTCTGTTGAACAAATCAACACGATGCTAAC
TAAAGAGTCTGGCCTACAAGGTCTAACTCAAGTGACTTCTGACTGTCGTTTCGTTGAAGACAACTACGGTGAGAAAGAAG
AAGCAACTCGTGCGATGGATGTATTCTGTCACCGTCTAGCTAAATACGTTGCAGGCTACACAGCAACACTAGAAGGTCGT
CTAGACGCAATCACTTTCACTGGCGGCATCGGTGAAAACTCTGCTCCAATCCGTGAAATGGTTCTTAACCGTTTAGGTAT
TTTCGGTATCGAAGTTGATAACGAAGCAAACCTTAAAGCACGTTTTGGTGGTGAAGGTACTATCACTACAGCAAACAGCC
GCGTTCCTGCTATGGTTATCTCGACTAACGAAGAGCTAGTAATCGCAGAAGACACAGCTAAACTAACTGGTCTATAA

Upstream 100 bases:

>100_bases
TTTTTGTACAAATGTGGGATTTTTCGTTAACTCACTACATTTGTCATGCTATATCGTTGCGATATAGCTTAATTACTGAT
GAATAAATAGGTATTTATAC

Downstream 100 bases:

>100_bases
TCTCTCCTAAAAGGCTAGCCACTAGGTTAGCCTTTTTTCTTTCTATGGCTTTATTCTTTAAATAAAGTCTCTTGCCCCAA
TAGTTTAGAGGAATTTTCCA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase 1 [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSKLVLVLNCGSSSLKFAVIDAESGAEHLTGLAECLGLPEARIKWKLDGKHEAQLGEGAAHEEALSFMVESILASKPELK
ANLGAIGHRIVHGGEQFTSSALITDEVLKGIQDAATFAPLHNPAHIIGIEAAKHNFPELQNVAVFDTAFHQTMPEESYLY
ALPYNLYTDHGIRRYGMHGTSHLFITREVAGLLNKPVEEVNIINCHLGNGASVCAIKNGQSVDTSMGLTPLEGLVMGTRC
GDIDPAIIFHLHDALGYSVEQINTMLTKESGLQGLTQVTSDCRFVEDNYGEKEEATRAMDVFCHRLAKYVAGYTATLEGR
LDAITFTGGIGENSAPIREMVLNRLGIFGIEVDNEANLKARFGGEGTITTANSRVPAMVISTNEELVIAEDTAKLTGL

Sequences:

>Translated_398_residues
MSKLVLVLNCGSSSLKFAVIDAESGAEHLTGLAECLGLPEARIKWKLDGKHEAQLGEGAAHEEALSFMVESILASKPELK
ANLGAIGHRIVHGGEQFTSSALITDEVLKGIQDAATFAPLHNPAHIIGIEAAKHNFPELQNVAVFDTAFHQTMPEESYLY
ALPYNLYTDHGIRRYGMHGTSHLFITREVAGLLNKPVEEVNIINCHLGNGASVCAIKNGQSVDTSMGLTPLEGLVMGTRC
GDIDPAIIFHLHDALGYSVEQINTMLTKESGLQGLTQVTSDCRFVEDNYGEKEEATRAMDVFCHRLAKYVAGYTATLEGR
LDAITFTGGIGENSAPIREMVLNRLGIFGIEVDNEANLKARFGGEGTITTANSRVPAMVISTNEELVIAEDTAKLTGL
>Mature_397_residues
SKLVLVLNCGSSSLKFAVIDAESGAEHLTGLAECLGLPEARIKWKLDGKHEAQLGEGAAHEEALSFMVESILASKPELKA
NLGAIGHRIVHGGEQFTSSALITDEVLKGIQDAATFAPLHNPAHIIGIEAAKHNFPELQNVAVFDTAFHQTMPEESYLYA
LPYNLYTDHGIRRYGMHGTSHLFITREVAGLLNKPVEEVNIINCHLGNGASVCAIKNGQSVDTSMGLTPLEGLVMGTRCG
DIDPAIIFHLHDALGYSVEQINTMLTKESGLQGLTQVTSDCRFVEDNYGEKEEATRAMDVFCHRLAKYVAGYTATLEGRL
DAITFTGGIGENSAPIREMVLNRLGIFGIEVDNEANLKARFGGEGTITTANSRVPAMVISTNEELVIAEDTAKLTGL

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=398, Percent_Identity=72.3618090452261, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI145693184, Length=397, Percent_Identity=45.5919395465995, Blast_Score=339, Evalue=2e-94,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 42860; Mature: 42729

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVLVLNCGSSSLKFAVIDAESGAEHLTGLAECLGLPEARIKWKLDGKHEAQLGEGAA
CCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCH
HEEALSFMVESILASKPELKANLGAIGHRIVHGGEQFTSSALITDEVLKGIQDAATFAPL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
HNPAHIIGIEAAKHNFPELQNVAVFDTAFHQTMPEESYLYALPYNLYTDHGIRRYGMHGT
CCCCEEEEEEHHCCCCCCCCCEEEEEHHHHHCCCCCCEEEEECCCHHCCCCCHHHCCCCC
SHLFITREVAGLLNKPVEEVNIINCHLGNGASVCAIKNGQSVDTSMGLTPLEGLVMGTRC
CEEEEHHHHHHHHHCCHHHEEEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEEECCC
GDIDPAIIFHLHDALGYSVEQINTMLTKESGLQGLTQVTSDCRFVEDNYGEKEEATRAMD
CCCCCCCEEEEHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCEEECCCCCCHHHHHHHHH
VFCHRLAKYVAGYTATLEGRLDAITFTGGIGENSAPIREMVLNRLGIFGIEVDNEANLKA
HHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCCEEE
RFGGEGTITTANSRVPAMVISTNEELVIAEDTAKLTGL
EECCCEEEEECCCCCCEEEEECCCCEEEEECCHHHCCC
>Mature Secondary Structure 
SKLVLVLNCGSSSLKFAVIDAESGAEHLTGLAECLGLPEARIKWKLDGKHEAQLGEGAA
CCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCH
HEEALSFMVESILASKPELKANLGAIGHRIVHGGEQFTSSALITDEVLKGIQDAATFAPL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
HNPAHIIGIEAAKHNFPELQNVAVFDTAFHQTMPEESYLYALPYNLYTDHGIRRYGMHGT
CCCCEEEEEEHHCCCCCCCCCEEEEEHHHHHCCCCCCEEEEECCCHHCCCCCHHHCCCCC
SHLFITREVAGLLNKPVEEVNIINCHLGNGASVCAIKNGQSVDTSMGLTPLEGLVMGTRC
CEEEEHHHHHHHHHCCHHHEEEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEEECCC
GDIDPAIIFHLHDALGYSVEQINTMLTKESGLQGLTQVTSDCRFVEDNYGEKEEATRAMD
CCCCCCCEEEEHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCEEECCCCCCHHHHHHHHH
VFCHRLAKYVAGYTATLEGRLDAITFTGGIGENSAPIREMVLNRLGIFGIEVDNEANLKA
HHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCCEEE
RFGGEGTITTANSRVPAMVISTNEELVIAEDTAKLTGL
EECCCEEEEECCCCCCEEEEECCCCEEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA