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The map label for this gene is vcmH [H]

Identifier: 209695472

GI number: 209695472

Start: 2164796

End: 2166175

Strand: Direct

Name: vcmH [H]

Synonym: VSAL_I2022

Alternate gene names: 209695472

Gene position: 2164796-2166175 (Clockwise)

Preceding gene: 209695471

Following gene: 209695473

Centisome position: 65.1

GC content: 41.96

Gene sequence:

>1380_bases
ATGTCTGTCTTCCTTTCTAATGTTATCGCACATACTCGCGGTGATTTCCTTAAGCGTTTAATTCTTATTGCAATACCTAT
TGCTCTGCAAAATATTATGTTCTCTAGTCGAGGCTTAGTGGACGTATTGATGCTGGGTCAATTAGGTGAAGCTGAGATTG
CTGCCGTTGGCGTAGCCAGCCGTGCTACGTTTGTAACAACGATAATGTTGGTTGGCGTGACCACGGGGGGCGCGTTGTTA
ACGGCTCAGTATTGGGGGGCACAAAATAGAGAAGGCGTTCGACAAAGTACGGCTCTGACTTGGATGGTGAGCATGGCAAT
GGCTACTATTGCCGTCGCTTTGTTTTTATTTATGCCTCATCAAATCATGTCATTAGCGACCGATTCTCAAGAGGTGATTG
ATTTAGGTGCGGATTACTTATTGATCACGGCACTGACGATGTACGTAGTGTCATGCGGCAGCAGTATGGCGGTAGGTTTA
CGATCGATCCATCAACCGGGTGTGAGTACTTTTTTTAGTGGTATTGGTATTGTTGCTAATGTCTTTTTTAACTGGGTACT
TATTTTTGGTCATTTAGGTGCTCCAGCAATGGGCATTAAAGGTGCGGCTTGGGCAACATTGATCAGTGGTGTTATTGAAA
TCGTATTTTTGTATGGTTACCTTTACCGTAAATCGCATTTATTAAGTTTTGGTTTTGATGACATTAAAGCGGTGATTAAT
TGGCCTAAGATAGCTAAGTTTTTATCACTTTCCTTACCGACTACGTTTAATTTTTTAGTTTGGGCGGCGGGTATTTTTAC
TTACCACGCGATTATGGGGCAATCTGGTGTTCAGGGCTTAGCGGCATTGTCTGTGATTTCTCCAATTGAATCTATTGCGT
TGAGTTTTCTTATTGGTGCAGCAGGTGCTGCCTCTGTGCTTATTGGTAATCAACTGGGTGCTAAGAAATATGAAGCCGTG
TATTACCAAGGGTGGGGAGTGCTTGCATTAAGTGTGGCCACCAGTGTTGTTATTGCTTTATTTGTGATGCTATTTCAACA
TCAGATTTTGGATATGTTTCCTGCGTTAAGTGACGAAACAAGAATGATTGCTGAAAAGTTCATGGCGATTTTAGCTGTGA
TTATTGTGATCCGCAGTGTGCCATTAACAGCTATTGTTGGCATTTTACGTGCGGGTGGTGATGTTAAGTTTTGCCTTTAC
CAAGACATTGTTTCTCAGTGGTTGATAGGGATCCCCGTTGCGACTATTGGTGCATTATTCTTAGGATTAGCCCCTGAATA
CGTTTATGCGTTATTTGTGGTTGAAGAAATTGTAAAATGGTTTGGATCTATCCATCGAATGAGATCGAAAAAATGGATTA
GAAATTTGGTGGATGCGTAA

Upstream 100 bases:

>100_bases
AATATTATTTACTTTCTGATTCTAGTACAATTTGTAGGCATATACCTAATGGATCTTATGCAGTGCAAATAGTTAATTTG
TTGTTTATCTGAGGTTCTGT

Downstream 100 bases:

>100_bases
TGAAATATAGATTTTTTCTTTATATATGAAGTAGATATACAAAAGGCTTTATGAAAGAAGGGTTTTGCTGTAAATTCTGT
TACGGATATCACAAATAATC

Product: multidrug efflux pump

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSVFLSNVIAHTRGDFLKRLILIAIPIALQNIMFSSRGLVDVLMLGQLGEAEIAAVGVASRATFVTTIMLVGVTTGGALL
TAQYWGAQNREGVRQSTALTWMVSMAMATIAVALFLFMPHQIMSLATDSQEVIDLGADYLLITALTMYVVSCGSSMAVGL
RSIHQPGVSTFFSGIGIVANVFFNWVLIFGHLGAPAMGIKGAAWATLISGVIEIVFLYGYLYRKSHLLSFGFDDIKAVIN
WPKIAKFLSLSLPTTFNFLVWAAGIFTYHAIMGQSGVQGLAALSVISPIESIALSFLIGAAGAASVLIGNQLGAKKYEAV
YYQGWGVLALSVATSVVIALFVMLFQHQILDMFPALSDETRMIAEKFMAILAVIIVIRSVPLTAIVGILRAGGDVKFCLY
QDIVSQWLIGIPVATIGALFLGLAPEYVYALFVVEEIVKWFGSIHRMRSKKWIRNLVDA

Sequences:

>Translated_459_residues
MSVFLSNVIAHTRGDFLKRLILIAIPIALQNIMFSSRGLVDVLMLGQLGEAEIAAVGVASRATFVTTIMLVGVTTGGALL
TAQYWGAQNREGVRQSTALTWMVSMAMATIAVALFLFMPHQIMSLATDSQEVIDLGADYLLITALTMYVVSCGSSMAVGL
RSIHQPGVSTFFSGIGIVANVFFNWVLIFGHLGAPAMGIKGAAWATLISGVIEIVFLYGYLYRKSHLLSFGFDDIKAVIN
WPKIAKFLSLSLPTTFNFLVWAAGIFTYHAIMGQSGVQGLAALSVISPIESIALSFLIGAAGAASVLIGNQLGAKKYEAV
YYQGWGVLALSVATSVVIALFVMLFQHQILDMFPALSDETRMIAEKFMAILAVIIVIRSVPLTAIVGILRAGGDVKFCLY
QDIVSQWLIGIPVATIGALFLGLAPEYVYALFVVEEIVKWFGSIHRMRSKKWIRNLVDA
>Mature_458_residues
SVFLSNVIAHTRGDFLKRLILIAIPIALQNIMFSSRGLVDVLMLGQLGEAEIAAVGVASRATFVTTIMLVGVTTGGALLT
AQYWGAQNREGVRQSTALTWMVSMAMATIAVALFLFMPHQIMSLATDSQEVIDLGADYLLITALTMYVVSCGSSMAVGLR
SIHQPGVSTFFSGIGIVANVFFNWVLIFGHLGAPAMGIKGAAWATLISGVIEIVFLYGYLYRKSHLLSFGFDDIKAVINW
PKIAKFLSLSLPTTFNFLVWAAGIFTYHAIMGQSGVQGLAALSVISPIESIALSFLIGAAGAASVLIGNQLGAKKYEAVY
YQGWGVLALSVATSVVIALFVMLFQHQILDMFPALSDETRMIAEKFMAILAVIIVIRSVPLTAIVGILRAGGDVKFCLYQ
DIVSQWLIGIPVATIGALFLGLAPEYVYALFVVEEIVKWFGSIHRMRSKKWIRNLVDA

Specific function: Unknown

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49565; Mature: 49433

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVFLSNVIAHTRGDFLKRLILIAIPIALQNIMFSSRGLVDVLMLGQLGEAEIAAVGVAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHH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HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]