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The map label for this gene is emrB [H]

Identifier: 209695468

GI number: 209695468

Start: 2158722

End: 2160326

Strand: Direct

Name: emrB [H]

Synonym: VSAL_I2017

Alternate gene names: 209695468

Gene position: 2158722-2160326 (Clockwise)

Preceding gene: 209695467

Following gene: 209695469

Centisome position: 64.92

GC content: 41.99

Gene sequence:

>1605_bases
ATGACTCAAGAATTAACAGCCACAAATCAAAATGACGTAGCGGATACACCGATAGAGCCTGAAATCCCAAGACGTCATTG
GATAGCTCTGTTCGGTGGCTTGCTTGGTGCGTTTATGGCGATTCTGGATATTCAGATCACCAACTCTTCACTAAAAGACA
TTCAAGGGGCGTTATCGGCCACCATGGATGAAAGTTCATGGATCTCTACCTCATACCTTGTGGCTGAAATGATCGCTATC
CCATTAAGTGGTTGGCTATCGGTCGCGATTGGTAAGCGTCGTTATTTAACGTGGACCACCATTATTTTTGCTGTGGCGTC
AGTCTTATGCTCACTCTCTTGGAGCATGGGATCGATGATCGTCTTTCGTGCGATCCAAGGCTTCAGTGGTGGCGCATTAA
TTCCTTTGGCGTTTTCTCTTGTTGTTCAATTATTACCATTGAATAAACGAGCGGTTGGTATGGCTCTCTTTGGTGTGACA
GCAACATTTGCACCATCAATCGGGCCAACATTGGGTGGCTGGTTAACGGAAAACTTTTCTTGGCATTATATTTTCTATAT
TAATATTCCCCCTGCGATAGCACTTATTTTTATGGTGAATTATGGTCTTGATGATGAACCGCTACGATTAGACAGTTTGC
TGAAAGCCGATTGGTTTGGCATCGCGACCATGGCGATAGGGTTGGGTTGTTTAGAAGTGGTTTTAGAAGAAGGGAATAGA
GAAGAGTGGTTCAGTTCTAGTTTCATTATTACGTTGTCGATTATCTCTGCCATTAGCTTAATTTATTTTGTGATTAACGA
GTTACAGCATAAAAACCCGTTAGTAAATTTGCGGCTACTTAAACAAAGTCAGTTTGCGATGTCGTGTGTCGCCTATTTAA
TTTTAGGGATGGCGTTATTGGGGTCTATTTACGTGTTGCCTATGTATATGATCCAAATTCAGGGTTATAACTCGTTAGAA
ATTGGTGAAGTATTAATGTGGATGGGGTTTCCTCAGTTATTAATTTTCCCATTGGTCCCTAAATTAACTCAGATTATTAA
AGCTAAATATCTCGTGTTTTTTGGTTTTGCAATGTTCGGTATTAGCTGCTTTGTAAACACACACATGAGTTATGATTTTG
GTGGCGATCAATTGATTTTCTCTATGCTGCTGCGTGCTATTGGTAGCCCATTTATTATGGTGCCGCTGTCTTTAGTTGCG
ATGGAAGAGATTCAAAAACATCAAGTGGCCGATGCCTCAACGATTACCAATGTACTGCGTAACTTGGGTGGTGCGTTTAG
TATTGCGTTTATTGCGACGCTGTTGGATAACAAAACCCGCGAACATTTGGGTCATATCAAAGAAACCTTAACTACGGTAA
GTACAGATGGGTGGTATCACTTGCAACAGAGTGCGGCGATGTTTGTCTCAAAGGGGAGTGATCCGGCAACGGCGATGCTG
CAAGCTAAAGCGTCATTGAGTCTGACAATGCAAAGAGACGCGGCAATTATGGCGTACAACGATGTGTTTTTCATGATGAC
ATGTTTCTTAGGTTTTGCTGCATTTATGATGTTTTTTGTTAAATCGAATTCGAATCCAGGGTCAATCGAGGCTGGAGGGC
ATTAA

Upstream 100 bases:

>100_bases
GAGTGCCGGTTAAAATTACTATTTTGAATGCTGATTTATTAGATGGTCAGTTAATTCCTGGGCTTTCTGTTGTTGCTACG
GTTGATACTCGAGGTTAATC

Downstream 100 bases:

>100_bases
TTTTTTATGGTTGCTCGCATCTTTAAGTGGTTTGGGTATAATGCGGCAGAATATTGAAATGAGGTTCTCATGCGTTTAGA
TAAATTTTTGTGCGAAACAA

Product: MFS transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MTQELTATNQNDVADTPIEPEIPRRHWIALFGGLLGAFMAILDIQITNSSLKDIQGALSATMDESSWISTSYLVAEMIAI
PLSGWLSVAIGKRRYLTWTTIIFAVASVLCSLSWSMGSMIVFRAIQGFSGGALIPLAFSLVVQLLPLNKRAVGMALFGVT
ATFAPSIGPTLGGWLTENFSWHYIFYINIPPAIALIFMVNYGLDDEPLRLDSLLKADWFGIATMAIGLGCLEVVLEEGNR
EEWFSSSFIITLSIISAISLIYFVINELQHKNPLVNLRLLKQSQFAMSCVAYLILGMALLGSIYVLPMYMIQIQGYNSLE
IGEVLMWMGFPQLLIFPLVPKLTQIIKAKYLVFFGFAMFGISCFVNTHMSYDFGGDQLIFSMLLRAIGSPFIMVPLSLVA
MEEIQKHQVADASTITNVLRNLGGAFSIAFIATLLDNKTREHLGHIKETLTTVSTDGWYHLQQSAAMFVSKGSDPATAML
QAKASLSLTMQRDAAIMAYNDVFFMMTCFLGFAAFMMFFVKSNSNPGSIEAGGH

Sequences:

>Translated_534_residues
MTQELTATNQNDVADTPIEPEIPRRHWIALFGGLLGAFMAILDIQITNSSLKDIQGALSATMDESSWISTSYLVAEMIAI
PLSGWLSVAIGKRRYLTWTTIIFAVASVLCSLSWSMGSMIVFRAIQGFSGGALIPLAFSLVVQLLPLNKRAVGMALFGVT
ATFAPSIGPTLGGWLTENFSWHYIFYINIPPAIALIFMVNYGLDDEPLRLDSLLKADWFGIATMAIGLGCLEVVLEEGNR
EEWFSSSFIITLSIISAISLIYFVINELQHKNPLVNLRLLKQSQFAMSCVAYLILGMALLGSIYVLPMYMIQIQGYNSLE
IGEVLMWMGFPQLLIFPLVPKLTQIIKAKYLVFFGFAMFGISCFVNTHMSYDFGGDQLIFSMLLRAIGSPFIMVPLSLVA
MEEIQKHQVADASTITNVLRNLGGAFSIAFIATLLDNKTREHLGHIKETLTTVSTDGWYHLQQSAAMFVSKGSDPATAML
QAKASLSLTMQRDAAIMAYNDVFFMMTCFLGFAAFMMFFVKSNSNPGSIEAGGH
>Mature_533_residues
TQELTATNQNDVADTPIEPEIPRRHWIALFGGLLGAFMAILDIQITNSSLKDIQGALSATMDESSWISTSYLVAEMIAIP
LSGWLSVAIGKRRYLTWTTIIFAVASVLCSLSWSMGSMIVFRAIQGFSGGALIPLAFSLVVQLLPLNKRAVGMALFGVTA
TFAPSIGPTLGGWLTENFSWHYIFYINIPPAIALIFMVNYGLDDEPLRLDSLLKADWFGIATMAIGLGCLEVVLEEGNRE
EWFSSSFIITLSIISAISLIYFVINELQHKNPLVNLRLLKQSQFAMSCVAYLILGMALLGSIYVLPMYMIQIQGYNSLEI
GEVLMWMGFPQLLIFPLVPKLTQIIKAKYLVFFGFAMFGISCFVNTHMSYDFGGDQLIFSMLLRAIGSPFIMVPLSLVAM
EEIQKHQVADASTITNVLRNLGGAFSIAFIATLLDNKTREHLGHIKETLTTVSTDGWYHLQQSAAMFVSKGSDPATAMLQ
AKASLSLTMQRDAAIMAYNDVFFMMTCFLGFAAFMMFFVKSNSNPGSIEAGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=475, Percent_Identity=28.8421052631579, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI1788710, Length=424, Percent_Identity=33.4905660377358, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1790195, Length=291, Percent_Identity=29.2096219931272, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081983, Length=435, Percent_Identity=23.6781609195402, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1788392, Length=274, Percent_Identity=27.007299270073, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6325455, Length=142, Percent_Identity=30.2816901408451, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=148, Percent_Identity=25.6756756756757, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=325, Percent_Identity=23.3846153846154, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=171, Percent_Identity=25.7309941520468, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 58798; Mature: 58667

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQELTATNQNDVADTPIEPEIPRRHWIALFGGLLGAFMAILDIQITNSSLKDIQGALSA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHH
TMDESSWISTSYLVAEMIAIPLSGWLSVAIGKRRYLTWTTIIFAVASVLCSLSWSMGSMI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFRAIQGFSGGALIPLAFSLVVQLLPLNKRAVGMALFGVTATFAPSIGPTLGGWLTENFS
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCC
WHYIFYINIPPAIALIFMVNYGLDDEPLRLDSLLKADWFGIATMAIGLGCLEVVLEEGNR
EEEEEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EEWFSSSFIITLSIISAISLIYFVINELQHKNPLVNLRLLKQSQFAMSCVAYLILGMALL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
GSIYVLPMYMIQIQGYNSLEIGEVLMWMGFPQLLIFPLVPKLTQIIKAKYLVFFGFAMFG
HHHHHHHHHHEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISCFVNTHMSYDFGGDQLIFSMLLRAIGSPFIMVPLSLVAMEEIQKHQVADASTITNVLR
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHH
NLGGAFSIAFIATLLDNKTREHLGHIKETLTTVSTDGWYHLQQSAAMFVSKGSDPATAML
HCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHH
QAKASLSLTMQRDAAIMAYNDVFFMMTCFLGFAAFMMFFVKSNSNPGSIEAGGH
HHHHHEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TQELTATNQNDVADTPIEPEIPRRHWIALFGGLLGAFMAILDIQITNSSLKDIQGALSA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHH
TMDESSWISTSYLVAEMIAIPLSGWLSVAIGKRRYLTWTTIIFAVASVLCSLSWSMGSMI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFRAIQGFSGGALIPLAFSLVVQLLPLNKRAVGMALFGVTATFAPSIGPTLGGWLTENFS
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCC
WHYIFYINIPPAIALIFMVNYGLDDEPLRLDSLLKADWFGIATMAIGLGCLEVVLEEGNR
EEEEEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EEWFSSSFIITLSIISAISLIYFVINELQHKNPLVNLRLLKQSQFAMSCVAYLILGMALL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
GSIYVLPMYMIQIQGYNSLEIGEVLMWMGFPQLLIFPLVPKLTQIIKAKYLVFFGFAMFG
HHHHHHHHHHEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISCFVNTHMSYDFGGDQLIFSMLLRAIGSPFIMVPLSLVAMEEIQKHQVADASTITNVLR
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHH
NLGGAFSIAFIATLLDNKTREHLGHIKETLTTVSTDGWYHLQQSAAMFVSKGSDPATAML
HCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHH
QAKASLSLTMQRDAAIMAYNDVFFMMTCFLGFAAFMMFFVKSNSNPGSIEAGGH
HHHHHEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]