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The map label for this gene is rsuA [H]

Identifier: 209695469

GI number: 209695469

Start: 2160396

End: 2161100

Strand: Direct

Name: rsuA [H]

Synonym: VSAL_I2018

Alternate gene names: 209695469

Gene position: 2160396-2161100 (Clockwise)

Preceding gene: 209695468

Following gene: 209695470

Centisome position: 64.97

GC content: 40.71

Gene sequence:

>705_bases
ATGCGTTTAGATAAATTTTTGTGCGAAACAACAGGTGCTACCCGTAGCGAAGCTACTCGAATCGTTAAAAGTGGTACTGT
CACTGTTAATGGCGTAAAAACAAAAGTGACTTCAGTAAAAGTAACTGACGATTCGACCGTTGAGTGGGATGGTCGTGAGC
TTAAATTCCATGGTCCTCGTTATATTATGCTATTTAAGCCAGATGGTTACGTGTGTTCACATGAAGACAGTACGCACCCA
TCGGCATTAACTCTTTTAGATGAAGTGAATCTAGAGAAGTTACACTTTGCTGGTCGCCTTGATGTTGATACGACAGGGTT
GGTACTGATTACTGATGATGGCCAATGGTCACATAAAATCACATCACCAAAGCGTAAGTGTCCTAAAACATACCGAGTAT
GGCTTGATGAGCCAATTGGTGCTTATTACGCTGAGCAATTAACGGAAGGCATTCAGCTTAAGAGTGAAACTGGTTTAATA
ATGCCTGCAACAATGGAAGTGGTTAATGAAGAAGAGCACGAGCTTTTATTAACGATCCAAGAAGGCAAGTATCATCAAGT
TAAACGTATGTTTGCTGCGTTGGGTAATAAAGTTGAAGGCTTACACCGTTCTCAAATTGGCGGTTTAGGTTTAGATTATT
CATTAGAGCCTGGCGAATATCGTTACTTAACCGCAGAAGAAGTAAAAGCGGTTTTGAGCAAGTAA

Upstream 100 bases:

>100_bases
TCCAGGGTCAATCGAGGCTGGAGGGCATTAATTTTTTATGGTTGCTCGCATCTTTAAGTGGTTTGGGTATAATGCGGCAG
AATATTGAAATGAGGTTCTC

Downstream 100 bases:

>100_bases
TTCACCCCAGTTATCTGTGGGTATAAAAAGCGGTGTGACAGAAATGTCATGCCGCTTTTTTTGTAAATTCTTGCAAAGAG
AACGAATATAAAAAGCATGT

Product: ribosomal small subunit pseudouridine synthase A (16S pseudouridine 516 synthase)

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MRLDKFLCETTGATRSEATRIVKSGTVTVNGVKTKVTSVKVTDDSTVEWDGRELKFHGPRYIMLFKPDGYVCSHEDSTHP
SALTLLDEVNLEKLHFAGRLDVDTTGLVLITDDGQWSHKITSPKRKCPKTYRVWLDEPIGAYYAEQLTEGIQLKSETGLI
MPATMEVVNEEEHELLLTIQEGKYHQVKRMFAALGNKVEGLHRSQIGGLGLDYSLEPGEYRYLTAEEVKAVLSK

Sequences:

>Translated_234_residues
MRLDKFLCETTGATRSEATRIVKSGTVTVNGVKTKVTSVKVTDDSTVEWDGRELKFHGPRYIMLFKPDGYVCSHEDSTHP
SALTLLDEVNLEKLHFAGRLDVDTTGLVLITDDGQWSHKITSPKRKCPKTYRVWLDEPIGAYYAEQLTEGIQLKSETGLI
MPATMEVVNEEEHELLLTIQEGKYHQVKRMFAALGNKVEGLHRSQIGGLGLDYSLEPGEYRYLTAEEVKAVLSK
>Mature_234_residues
MRLDKFLCETTGATRSEATRIVKSGTVTVNGVKTKVTSVKVTDDSTVEWDGRELKFHGPRYIMLFKPDGYVCSHEDSTHP
SALTLLDEVNLEKLHFAGRLDVDTTGLVLITDDGQWSHKITSPKRKCPKTYRVWLDEPIGAYYAEQLTEGIQLKSETGLI
MPATMEVVNEEEHELLLTIQEGKYHQVKRMFAALGNKVEGLHRSQIGGLGLDYSLEPGEYRYLTAEEVKAVLSK

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=233, Percent_Identity=52.7896995708155, Blast_Score=244, Evalue=2e-66,
Organism=Escherichia coli, GI1787524, Length=231, Percent_Identity=28.1385281385281, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1790453, Length=235, Percent_Identity=28.0851063829787, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 26274; Mature: 26274

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFLCETTGATRSEATRIVKSGTVTVNGVKTKVTSVKVTDDSTVEWDGRELKFHGPR
CCHHHHHHHCCCCCHHHHHHHEECCEEEEECEEEEEEEEEECCCCEEEECCCEEEEECCE
YIMLFKPDGYVCSHEDSTHPSALTLLDEVNLEKLHFAGRLDVDTTGLVLITDDGQWSHKI
EEEEECCCCEEECCCCCCCCHHEEEEHHCCHHHEEECCCCCCCCCCEEEEECCCCCCCCC
TSPKRKCPKTYRVWLDEPIGAYYAEQLTEGIQLKSETGLIMPATMEVVNEEEHELLLTIQ
CCCHHHCCCEEEEEECCCCHHHHHHHHHHCCEEECCCCEEEEHHHHHHCCCCCEEEEEEE
EGKYHQVKRMFAALGNKVEGLHRSQIGGLGLDYSLEPGEYRYLTAEEVKAVLSK
CCCHHHHHHHHHHHCCHHCCHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHCC
>Mature Secondary Structure
MRLDKFLCETTGATRSEATRIVKSGTVTVNGVKTKVTSVKVTDDSTVEWDGRELKFHGPR
CCHHHHHHHCCCCCHHHHHHHEECCEEEEECEEEEEEEEEECCCCEEEECCCEEEEECCE
YIMLFKPDGYVCSHEDSTHPSALTLLDEVNLEKLHFAGRLDVDTTGLVLITDDGQWSHKI
EEEEECCCCEEECCCCCCCCHHEEEEHHCCHHHEEECCCCCCCCCCEEEEECCCCCCCCC
TSPKRKCPKTYRVWLDEPIGAYYAEQLTEGIQLKSETGLIMPATMEVVNEEEHELLLTIQ
CCCHHHCCCEEEEEECCCCHHHHHHHHHHCCEEECCCCEEEEHHHHHHCCCCCEEEEEEE
EGKYHQVKRMFAALGNKVEGLHRSQIGGLGLDYSLEPGEYRYLTAEEVKAVLSK
CCCHHHHHHHHHHHCCHHCCHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA