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The map label for this gene is rluF [H]

Identifier: 209695446

GI number: 209695446

Start: 2129295

End: 2130239

Strand: Direct

Name: rluF [H]

Synonym: VSAL_I1987

Alternate gene names: 209695446

Gene position: 2129295-2130239 (Clockwise)

Preceding gene: 209695445

Following gene: 209695447

Centisome position: 64.04

GC content: 37.67

Gene sequence:

>945_bases
ATGACATCACCAGAACCTAAACTAACTCGTTTAAACAAATACATTAGTGATACGGGTTATTGCTCACGAAGAGAAGCTGA
TAAATTGATTGATCAGCAGCGAATTACTATTAATGGCAACGTTCCTGAAATGGGTACAAAAGTAGCTGAAGGTGATGATG
TTCTGGTTGATGGAAAACCATTGAAAGCAAAGCAAGAACGTATCTATATTGCATTCAATAAGCCAGTCGGTATTACCTGT
ACAAGCGAACGTCATGTCGAAGGTAATATTATTGATTATATTAACCACGAACAGCGTATTTTTCCTATTGGTCGATTGGA
CAAGCCGTCTGATGGTTTGATTTTCTTAACCAGTGATGGAGATATCGTTAATAAGATTCTTCGTGCGGGCAATGCGCATG
AAAAAGAATATGTCGTTCGTGTTGATAAACCTATTACTGATGACTTTCTAGCACAAATGGCGGCGGGTGTTCCTATTTTA
GATACCGTAACGCTACCTTGTCAGGTTAAACAAGAATCACGTTTTGTTTTCCGAATTACGTTAACGCAAGGTTTAAATCG
TCAAATTCGTCGTATGTGTGAATACTTAGATTATGAAGTATTTAAACTTAAACGCGTTCGAATTATGAATATTGACTTAG
GCACACTTGCGTCTGGTGAATGGCGTTATTTAACGGCTTCAGAGATGGAACAAATCAATAATATGATTGGTACATCAAGT
AAGACAGAAGAAGCGTCACGTAAAGTGAAAGAAGTTGATGGGAAAATTAGGACCATCAAAAAGGCAAGTTCTGGACGTAA
TCATCATTCGAATAATACATTTAGTCAAAAACGCCGTGAAGAATCGAAAGATCGTGGTTTTGGTAAATCGTACTCTCAAC
GAAGAGATGAGGGTAAAGATCAAGATAAACCGCAGTCGACTATTAAAGTATGGCGAGCAAAATAA

Upstream 100 bases:

>100_bases
ATTATAATTAAGCGTTCTAAACTACGGTCTAGAGCGCTTTTTTTATTTATTTTTTAAAGTTTCTGATACACTCCGGCTGT
TCATTTTTTTGAGGTACACC

Downstream 100 bases:

>100_bases
GTTTGCTTTCATCATCTTAGGGTGAATAATGTACGGATTGATGGCAAAGTATTCAAACGAAATAAAAAACACAATTTTTG
TTTGACTCTTTTTTTAAATC

Product: ribosomal large subunit pseudouridine synthase F

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTSPEPKLTRLNKYISDTGYCSRREADKLIDQQRITINGNVPEMGTKVAEGDDVLVDGKPLKAKQERIYIAFNKPVGITC
TSERHVEGNIIDYINHEQRIFPIGRLDKPSDGLIFLTSDGDIVNKILRAGNAHEKEYVVRVDKPITDDFLAQMAAGVPIL
DTVTLPCQVKQESRFVFRITLTQGLNRQIRRMCEYLDYEVFKLKRVRIMNIDLGTLASGEWRYLTASEMEQINNMIGTSS
KTEEASRKVKEVDGKIRTIKKASSGRNHHSNNTFSQKRREESKDRGFGKSYSQRRDEGKDQDKPQSTIKVWRAK

Sequences:

>Translated_314_residues
MTSPEPKLTRLNKYISDTGYCSRREADKLIDQQRITINGNVPEMGTKVAEGDDVLVDGKPLKAKQERIYIAFNKPVGITC
TSERHVEGNIIDYINHEQRIFPIGRLDKPSDGLIFLTSDGDIVNKILRAGNAHEKEYVVRVDKPITDDFLAQMAAGVPIL
DTVTLPCQVKQESRFVFRITLTQGLNRQIRRMCEYLDYEVFKLKRVRIMNIDLGTLASGEWRYLTASEMEQINNMIGTSS
KTEEASRKVKEVDGKIRTIKKASSGRNHHSNNTFSQKRREESKDRGFGKSYSQRRDEGKDQDKPQSTIKVWRAK
>Mature_313_residues
TSPEPKLTRLNKYISDTGYCSRREADKLIDQQRITINGNVPEMGTKVAEGDDVLVDGKPLKAKQERIYIAFNKPVGITCT
SERHVEGNIIDYINHEQRIFPIGRLDKPSDGLIFLTSDGDIVNKILRAGNAHEKEYVVRVDKPITDDFLAQMAAGVPILD
TVTLPCQVKQESRFVFRITLTQGLNRQIRRMCEYLDYEVFKLKRVRIMNIDLGTLASGEWRYLTASEMEQINNMIGTSSK
TEEASRKVKEVDGKIRTIKKASSGRNHHSNNTFSQKRREESKDRGFGKSYSQRRDEGKDQDKPQSTIKVWRAK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=234, Percent_Identity=57.6923076923077, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1788510, Length=229, Percent_Identity=30.1310043668122, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1787524, Length=286, Percent_Identity=27.972027972028, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081838, Length=175, Percent_Identity=29.1428571428571, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 35885; Mature: 35754

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPEPKLTRLNKYISDTGYCSRREADKLIDQQRITINGNVPEMGTKVAEGDDVLVDGKP
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCCCHHCCEECCCCEEEECCCC
LKAKQERIYIAFNKPVGITCTSERHVEGNIIDYINHEQRIFPIGRLDKPSDGLIFLTSDG
CCCCCCEEEEEECCCEEEEECCCCCCCCCEEECCCCCCEEEECCCCCCCCCCEEEEECCC
DIVNKILRAGNAHEKEYVVRVDKPITDDFLAQMAAGVPILDTVTLPCQVKQESRFVFRIT
HHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCEEECCCCCEEEEEE
LTQGLNRQIRRMCEYLDYEVFKLKRVRIMNIDLGTLASGEWRYLTASEMEQINNMIGTSS
EHHCCCHHHHHHHHHHCHHHEEEEEEEEEEECCCCCCCCCEEEECHHHHHHHHHHHCCCC
KTEEASRKVKEVDGKIRTIKKASSGRNHHSNNTFSQKRREESKDRGFGKSYSQRRDEGKD
CHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCC
QDKPQSTIKVWRAK
CCCCCHHHEEEECC
>Mature Secondary Structure 
TSPEPKLTRLNKYISDTGYCSRREADKLIDQQRITINGNVPEMGTKVAEGDDVLVDGKP
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCCCHHCCEECCCCEEEECCCC
LKAKQERIYIAFNKPVGITCTSERHVEGNIIDYINHEQRIFPIGRLDKPSDGLIFLTSDG
CCCCCCEEEEEECCCEEEEECCCCCCCCCEEECCCCCCEEEECCCCCCCCCCEEEEECCC
DIVNKILRAGNAHEKEYVVRVDKPITDDFLAQMAAGVPILDTVTLPCQVKQESRFVFRIT
HHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCEEECCCCCEEEEEE
LTQGLNRQIRRMCEYLDYEVFKLKRVRIMNIDLGTLASGEWRYLTASEMEQINNMIGTSS
EHHCCCHHHHHHHHHHCHHHEEEEEEEEEEECCCCCCCCCEEEECHHHHHHHHHHHCCCC
KTEEASRKVKEVDGKIRTIKKASSGRNHHSNNTFSQKRREESKDRGFGKSYSQRRDEGKD
CHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCC
QDKPQSTIKVWRAK
CCCCCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]