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The map label for this gene is cysS

Identifier: 209695403

GI number: 209695403

Start: 2074764

End: 2076152

Strand: Direct

Name: cysS

Synonym: VSAL_I1940

Alternate gene names: 209695403

Gene position: 2074764-2076152 (Clockwise)

Preceding gene: 209695398

Following gene: 209695404

Centisome position: 62.4

GC content: 40.1

Gene sequence:

>1389_bases
ATGTTGAAAATATATAATTCCCTAACACGCCAAAAAGAAGAATTTAAACCAATTACCGAAGGTAAAGTCGGTATGTATGT
GTGTGGGGTTACGATTTACGATTTGTGTCATATTGGCCATGGTCGTACTTTTGTATCTTTTGATGTGATTTCTCGTTACT
TACGTTTTTTAGGCTACGATCTTACATTTGTTCGTAACATCACTGATATTGACGATAAAATCATTAAACGTGCTGCTGAA
AACAACGAAAGCTGTGAAGCACTAACTGAACGTTTGATTGCAGAAATGCATGCGGATTTTGATGCTTTGAAAATGAAACG
TCCAGATGTTGAACCACGAGCGACTGAATACATTACGGAAATTGTAGAATTAGTTGAGCGCTTAATTGAACGTGGTTTTG
CATACGTTGCTTCAAATGGCGATGTAATGTTTGAAGTAAAGAAATTTGATGAATATGGTCGTCTTTCTCGTCAAGATCTT
GAACAGCTTCAAGCGGGCTCTCGTGTGACTCTTGAAGAAACAAGTGTTAAGCGCAGTGGAATGGATTTTGTTTTATGGAA
AATGTCTAAACCAGGTGAGCCAACATGGGAATCTCCTTGGGGTCCAGGTCGTCCAGGTTGGCACATTGAATGTTCTGCAA
TGAACTCTTCTATTCTAGGTAATCATTTTGATATACATGGTGGGGGTTCTGATCTTATGTTCCCACATCATGAGAATGAA
ATCGCTCAATCATGTTGTGCGCATGACGCTAAGTACGTAAATACTTGGATGCACAGTGGTATGGTGATGATTGATCGTGA
AAAGATGTCTAAGTCATTAGGCAACTTCTTTACTATCCGTGACGTATTAGCGCATTACGATTCAGAAACGGTTCGTTACT
TTTTAATGTCTGGCCATTACCGTAGTCAATTGAACTACAGCGAAGAAAATTTAAATCAAGCTCGTTCTTCATTAGAGCGT
CTATATACTTCGTTACGTGGCCTTGATTTAACGGTGACACCTGCGGGTGGTGAAACGTTTGTTACTCGTTTCTCTACGGC
AATGAATGATGATTTCAATACACCAGAAGCTTATTCAGTGTTGTTTGAAATGGCACGCGAAGTAAACCGTTTAAAAACCG
AAAATATCGAAGCCGCATCGAAACTTGGTGCATTAATGCGTGAATTGGCTGAGGTATTAGGTTTATTGTCACAAGAACCA
GAAGCTTTCTTACAAGGTGATAGCGGCAGTGATAATGAAGTTGCTGAAATAGAAGCATTGATCAAAGCGCGTAACGATGC
TCGTGCAGCAAAAGATTGGTCAGCAGCCGATGCCGCTCGTGATGCCATCACAGCGTTAAATATTGTATTAGAAGATGGCC
CAGAAGGAACAACTTGGCGTCGTAAATAA

Upstream 100 bases:

>100_bases
ATTAAGTCGGATCTACAGGGAATTTTCTTGCTATCATTACTTATTATAGGTTTAATAGCCTTTATATATCCTTAAAACAA
ATAACGAGTAAAAAAAGAAC

Downstream 100 bases:

>100_bases
TATAAACCGTTTTATGTAAAGGGCTGGTCTTTCCAGCCTTTTTATTACCAAATTTTTAAACTTTAATAGAGGACACATTT
GTGGCTCAAATGTACTTTTA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MLKIYNSLTRQKEEFKPITEGKVGMYVCGVTIYDLCHIGHGRTFVSFDVISRYLRFLGYDLTFVRNITDIDDKIIKRAAE
NNESCEALTERLIAEMHADFDALKMKRPDVEPRATEYITEIVELVERLIERGFAYVASNGDVMFEVKKFDEYGRLSRQDL
EQLQAGSRVTLEETSVKRSGMDFVLWKMSKPGEPTWESPWGPGRPGWHIECSAMNSSILGNHFDIHGGGSDLMFPHHENE
IAQSCCAHDAKYVNTWMHSGMVMIDREKMSKSLGNFFTIRDVLAHYDSETVRYFLMSGHYRSQLNYSEENLNQARSSLER
LYTSLRGLDLTVTPAGGETFVTRFSTAMNDDFNTPEAYSVLFEMAREVNRLKTENIEAASKLGALMRELAEVLGLLSQEP
EAFLQGDSGSDNEVAEIEALIKARNDARAAKDWSAADAARDAITALNIVLEDGPEGTTWRRK

Sequences:

>Translated_462_residues
MLKIYNSLTRQKEEFKPITEGKVGMYVCGVTIYDLCHIGHGRTFVSFDVISRYLRFLGYDLTFVRNITDIDDKIIKRAAE
NNESCEALTERLIAEMHADFDALKMKRPDVEPRATEYITEIVELVERLIERGFAYVASNGDVMFEVKKFDEYGRLSRQDL
EQLQAGSRVTLEETSVKRSGMDFVLWKMSKPGEPTWESPWGPGRPGWHIECSAMNSSILGNHFDIHGGGSDLMFPHHENE
IAQSCCAHDAKYVNTWMHSGMVMIDREKMSKSLGNFFTIRDVLAHYDSETVRYFLMSGHYRSQLNYSEENLNQARSSLER
LYTSLRGLDLTVTPAGGETFVTRFSTAMNDDFNTPEAYSVLFEMAREVNRLKTENIEAASKLGALMRELAEVLGLLSQEP
EAFLQGDSGSDNEVAEIEALIKARNDARAAKDWSAADAARDAITALNIVLEDGPEGTTWRRK
>Mature_462_residues
MLKIYNSLTRQKEEFKPITEGKVGMYVCGVTIYDLCHIGHGRTFVSFDVISRYLRFLGYDLTFVRNITDIDDKIIKRAAE
NNESCEALTERLIAEMHADFDALKMKRPDVEPRATEYITEIVELVERLIERGFAYVASNGDVMFEVKKFDEYGRLSRQDL
EQLQAGSRVTLEETSVKRSGMDFVLWKMSKPGEPTWESPWGPGRPGWHIECSAMNSSILGNHFDIHGGGSDLMFPHHENE
IAQSCCAHDAKYVNTWMHSGMVMIDREKMSKSLGNFFTIRDVLAHYDSETVRYFLMSGHYRSQLNYSEENLNQARSSLER
LYTSLRGLDLTVTPAGGETFVTRFSTAMNDDFNTPEAYSVLFEMAREVNRLKTENIEAASKLGALMRELAEVLGLLSQEP
EAFLQGDSGSDNEVAEIEALIKARNDARAAKDWSAADAARDAITALNIVLEDGPEGTTWRRK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=481, Percent_Identity=35.5509355509356, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI62240992, Length=233, Percent_Identity=43.7768240343348, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI303304987, Length=233, Percent_Identity=43.7768240343348, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI10835051, Length=233, Percent_Identity=43.7768240343348, Blast_Score=199, Evalue=6e-51,
Organism=Homo sapiens, GI21269877, Length=233, Percent_Identity=43.7768240343348, Blast_Score=199, Evalue=6e-51,
Organism=Escherichia coli, GI1786737, Length=463, Percent_Identity=74.7300215982721, Blast_Score=709, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17509795, Length=238, Percent_Identity=42.8571428571429, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17509797, Length=238, Percent_Identity=42.8571428571429, Blast_Score=182, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6324082, Length=402, Percent_Identity=32.089552238806, Blast_Score=206, Evalue=6e-54,
Organism=Drosophila melanogaster, GI19922218, Length=501, Percent_Identity=33.1337325349301, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI19922364, Length=344, Percent_Identity=36.3372093023256, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_ALISL (B6EH43)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263332.1
- GeneID:   6989001
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I1940
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52335; Mature: 52335

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIYNSLTRQKEEFKPITEGKVGMYVCGVTIYDLCHIGHGRTFVSFDVISRYLRFLGYD
CCCHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCC
LTFVRNITDIDDKIIKRAAENNESCEALTERLIAEMHADFDALKMKRPDVEPRATEYITE
HHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHH
IVELVERLIERGFAYVASNGDVMFEVKKFDEYGRLSRQDLEQLQAGSRVTLEETSVKRSG
HHHHHHHHHHCCCEEEECCCCEEEEEEHHHHHCCCCHHHHHHHHCCCCEEEHHHHHHHCC
MDFVLWKMSKPGEPTWESPWGPGRPGWHIECSAMNSSILGNHFDIHGGGSDLMFPHHENE
CCEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCCCCCCCCCCHHH
IAQSCCAHDAKYVNTWMHSGMVMIDREKMSKSLGNFFTIRDVLAHYDSETVRYFLMSGHY
HHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCH
RSQLNYSEENLNQARSSLERLYTSLRGLDLTVTPAGGETFVTRFSTAMNDDFNTPEAYSV
HHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHH
LFEMAREVNRLKTENIEAASKLGALMRELAEVLGLLSQEPEAFLQGDSGSDNEVAEIEAL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHH
IKARNDARAAKDWSAADAARDAITALNIVLEDGPEGTTWRRK
HHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCCCCCCCC
>Mature Secondary Structure
MLKIYNSLTRQKEEFKPITEGKVGMYVCGVTIYDLCHIGHGRTFVSFDVISRYLRFLGYD
CCCHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCC
LTFVRNITDIDDKIIKRAAENNESCEALTERLIAEMHADFDALKMKRPDVEPRATEYITE
HHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHH
IVELVERLIERGFAYVASNGDVMFEVKKFDEYGRLSRQDLEQLQAGSRVTLEETSVKRSG
HHHHHHHHHHCCCEEEECCCCEEEEEEHHHHHCCCCHHHHHHHHCCCCEEEHHHHHHHCC
MDFVLWKMSKPGEPTWESPWGPGRPGWHIECSAMNSSILGNHFDIHGGGSDLMFPHHENE
CCEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCCCCCCCCCCHHH
IAQSCCAHDAKYVNTWMHSGMVMIDREKMSKSLGNFFTIRDVLAHYDSETVRYFLMSGHY
HHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCH
RSQLNYSEENLNQARSSLERLYTSLRGLDLTVTPAGGETFVTRFSTAMNDDFNTPEAYSV
HHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHH
LFEMAREVNRLKTENIEAASKLGALMRELAEVLGLLSQEPEAFLQGDSGSDNEVAEIEAL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHH
IKARNDARAAKDWSAADAARDAITALNIVLEDGPEGTTWRRK
HHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA