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The map label for this gene is tolB

Identifier: 209695365

GI number: 209695365

Start: 2031480

End: 2032832

Strand: Direct

Name: tolB

Synonym: VSAL_I1898

Alternate gene names: 209695365

Gene position: 2031480-2032832 (Clockwise)

Preceding gene: 209695364

Following gene: 209695366

Centisome position: 61.09

GC content: 39.39

Gene sequence:

>1353_bases
GTGCTTAAACGATGGATATTACCTCTTTTTATAGCGTGTTTGAGTTTTAGCCAAATAGCTAAAGCTGAACTAGAGCTTGT
AATCACAGAGGGCGTAAATTCTGCTAGACCCATAGGCATCGTTCCATTTAAGTGGCATGGTGAAGGCAAGTTACCACAAG
ATATTTCAGCTATTATTTCTTCTGACTTACAGCGCAGTGGTAAATTTAGTCCATTACCGACAAATAAAATGCCACAAACG
CCATATAAAGATGCTGACATTAATTACGAAGCTTGGACCAAGATGGGCGTTGATGCCATTGTTACGGGTGAAGTGAAAGT
TAATGCCGCTGGAAAGTATGAGGTTTCTTATAAGCTTATTGATGTGGTTCGTGGTCAATTAACGAAAGGTAAATCTAAAG
GGTTAAGTAATTCAGGGGAATTAGTTTTAACACAAGACCATATTTTAGTTAATAAAATTGCGACACTTTCAAAAACACAG
TTACGAAAATACGCTCACCGTATTTCTGATGTTGTTTATGAAAAATTAACAGGCGAAAAAGGCGCGTTTTTAACGCGAAT
TGCTTATGTGGTTGTTAATGATAAATCTCAACATCCATATCAGTTAAGAATTGCGGATTATGACGGCTACAACGAACGTT
TGGTTTTAAAGTCGAAACAGCCAATCATGTCACCGGCTTGGTCTCCAGATGGTAAGAAATTGGCTTATGTAAGTTTTGAA
AACAGAAGATCACAAGTTTTCATTATGAATATCTACACGGGGAAGAGGGAGTTAATTGCTTCGTATCCTCGTCATAACGG
TGCGCCTCGTTTTTCTCATGACGGAAAAGAACTTGCGATCGTATTATCCAAAACCGGCAGTCTTCAAGTTTATATTGTGA
ATTTACAAACGAAAAAGATGCGCCAAATTACTCGAGGTAGATCGAATAATACTGAGCCTTTTTGGGCTCCTGATAACAAG
TCTTTAATATTTACATCAGATCGGGGTGGTAAACCTCAGATTTATAGAGTAAATTTAGAAGACGGTTCGACAAAACGTTT
AACCTGGCAAGGCAGTCAAAATCTTGGTGGGCAGATCACTCCTGATGGTAAATACATCGTTATGGTGAATCGCTCAGAGA
CTGGATTTAATCTAGCAAAACAAGATTTAGAAACCGGTGCTGTCCAGGTCTTAACTAAGACACTACTGGATGAGTCACCA
AGTATTGCGCCAAATGGCGGCATGGTGATTTACAGTTCCATTTATAGAAAACAAAACGTCCTTTCGATGGTTTCTATAGA
TGGCCGTTTTAAAGCTCGCTTACCAGCGACAAACGGACGTGTAAGGGCACCAGCGTGGTCGCCATTTTTATAG

Upstream 100 bases:

>100_bases
GTAACTAAAGTTAATGTTTTTCAAATGCCAAAAGACGCTGATATTGTAGCTAAACTTACAAATATAAACCTTACGGTTCA
ACCAGAATAAGGAAGAGCAA

Downstream 100 bases:

>100_bases
CTACTAAATATTTAGGAAATAAGGAAAATATAATGCAACTAAATAAACTTCTTAAGAGTCTTTTAATCGCGCTGCCAGTG
GTTGCTCTTTCTGCTTGTAG

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLKRWILPLFIACLSFSQIAKAELELVITEGVNSARPIGIVPFKWHGEGKLPQDISAIISSDLQRSGKFSPLPTNKMPQT
PYKDADINYEAWTKMGVDAIVTGEVKVNAAGKYEVSYKLIDVVRGQLTKGKSKGLSNSGELVLTQDHILVNKIATLSKTQ
LRKYAHRISDVVYEKLTGEKGAFLTRIAYVVVNDKSQHPYQLRIADYDGYNERLVLKSKQPIMSPAWSPDGKKLAYVSFE
NRRSQVFIMNIYTGKRELIASYPRHNGAPRFSHDGKELAIVLSKTGSLQVYIVNLQTKKMRQITRGRSNNTEPFWAPDNK
SLIFTSDRGGKPQIYRVNLEDGSTKRLTWQGSQNLGGQITPDGKYIVMVNRSETGFNLAKQDLETGAVQVLTKTLLDESP
SIAPNGGMVIYSSIYRKQNVLSMVSIDGRFKARLPATNGRVRAPAWSPFL

Sequences:

>Translated_450_residues
MLKRWILPLFIACLSFSQIAKAELELVITEGVNSARPIGIVPFKWHGEGKLPQDISAIISSDLQRSGKFSPLPTNKMPQT
PYKDADINYEAWTKMGVDAIVTGEVKVNAAGKYEVSYKLIDVVRGQLTKGKSKGLSNSGELVLTQDHILVNKIATLSKTQ
LRKYAHRISDVVYEKLTGEKGAFLTRIAYVVVNDKSQHPYQLRIADYDGYNERLVLKSKQPIMSPAWSPDGKKLAYVSFE
NRRSQVFIMNIYTGKRELIASYPRHNGAPRFSHDGKELAIVLSKTGSLQVYIVNLQTKKMRQITRGRSNNTEPFWAPDNK
SLIFTSDRGGKPQIYRVNLEDGSTKRLTWQGSQNLGGQITPDGKYIVMVNRSETGFNLAKQDLETGAVQVLTKTLLDESP
SIAPNGGMVIYSSIYRKQNVLSMVSIDGRFKARLPATNGRVRAPAWSPFL
>Mature_450_residues
MLKRWILPLFIACLSFSQIAKAELELVITEGVNSARPIGIVPFKWHGEGKLPQDISAIISSDLQRSGKFSPLPTNKMPQT
PYKDADINYEAWTKMGVDAIVTGEVKVNAAGKYEVSYKLIDVVRGQLTKGKSKGLSNSGELVLTQDHILVNKIATLSKTQ
LRKYAHRISDVVYEKLTGEKGAFLTRIAYVVVNDKSQHPYQLRIADYDGYNERLVLKSKQPIMSPAWSPDGKKLAYVSFE
NRRSQVFIMNIYTGKRELIASYPRHNGAPRFSHDGKELAIVLSKTGSLQVYIVNLQTKKMRQITRGRSNNTEPFWAPDNK
SLIFTSDRGGKPQIYRVNLEDGSTKRLTWQGSQNLGGQITPDGKYIVMVNRSETGFNLAKQDLETGAVQVLTKTLLDESP
SIAPNGGMVIYSSIYRKQNVLSMVSIDGRFKARLPATNGRVRAPAWSPFL

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=449, Percent_Identity=50.5567928730512, Blast_Score=468, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_ALISL (B6EGK3)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263294.1
- ProteinModelPortal:   B6EGK3
- GeneID:   6989303
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I1898
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- ProtClustDB:   PRK04792
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 50272; Mature: 50272

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRWILPLFIACLSFSQIAKAELELVITEGVNSARPIGIVPFKWHGEGKLPQDISAIIS
CCHHHHHHHHHHHHCHHHHCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCHHHHHHHH
SDLQRSGKFSPLPTNKMPQTPYKDADINYEAWTKMGVDAIVTGEVKVNAAGKYEVSYKLI
HHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCEEEECCCCEEEEEEEE
DVVRGQLTKGKSKGLSNSGELVLTQDHILVNKIATLSKTQLRKYAHRISDVVYEKLTGEK
HHHHHHCCCCCCCCCCCCCCEEEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GAFLTRIAYVVVNDKSQHPYQLRIADYDGYNERLVLKSKQPIMSPAWSPDGKKLAYVSFE
CCEEEEEEEEEEECCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEC
NRRSQVFIMNIYTGKRELIASYPRHNGAPRFSHDGKELAIVLSKTGSLQVYIVNLQTKKM
CCCCEEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCHHHH
RQITRGRSNNTEPFWAPDNKSLIFTSDRGGKPQIYRVNLEDGSTKRLTWQGSQNLGGQIT
HHHHCCCCCCCCCEECCCCCEEEEECCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEC
PDGKYIVMVNRSETGFNLAKQDLETGAVQVLTKTLLDESPSIAPNGGMVIYSSIYRKQNV
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCC
LSMVSIDGRFKARLPATNGRVRAPAWSPFL
EEEEEECCEEEEECCCCCCEEECCCCCCCC
>Mature Secondary Structure
MLKRWILPLFIACLSFSQIAKAELELVITEGVNSARPIGIVPFKWHGEGKLPQDISAIIS
CCHHHHHHHHHHHHCHHHHCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCHHHHHHHH
SDLQRSGKFSPLPTNKMPQTPYKDADINYEAWTKMGVDAIVTGEVKVNAAGKYEVSYKLI
HHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCEEEECCCCEEEEEEEE
DVVRGQLTKGKSKGLSNSGELVLTQDHILVNKIATLSKTQLRKYAHRISDVVYEKLTGEK
HHHHHHCCCCCCCCCCCCCCEEEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GAFLTRIAYVVVNDKSQHPYQLRIADYDGYNERLVLKSKQPIMSPAWSPDGKKLAYVSFE
CCEEEEEEEEEEECCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEC
NRRSQVFIMNIYTGKRELIASYPRHNGAPRFSHDGKELAIVLSKTGSLQVYIVNLQTKKM
CCCCEEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCHHHH
RQITRGRSNNTEPFWAPDNKSLIFTSDRGGKPQIYRVNLEDGSTKRLTWQGSQNLGGQIT
HHHHCCCCCCCCCEECCCCCEEEEECCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEC
PDGKYIVMVNRSETGFNLAKQDLETGAVQVLTKTLLDESPSIAPNGGMVIYSSIYRKQNV
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCC
LSMVSIDGRFKARLPATNGRVRAPAWSPFL
EEEEEECCEEEEECCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA