Click here to switch to the map view.

The map label for this gene is tolA [C]

Identifier: 209695364

GI number: 209695364

Start: 2030462

End: 2031469

Strand: Direct

Name: tolA [C]

Synonym: VSAL_I1897

Alternate gene names: 209695364

Gene position: 2030462-2031469 (Clockwise)

Preceding gene: 209695363

Following gene: 209695365

Centisome position: 61.06

GC content: 40.18

Gene sequence:

>1008_bases
ATGAAATCGGATAATAATTACACCTTTGCTATTGTTGTTTCTGTCGTGTTGCACTTGCTGCTCGCTGTCGCGTTGATCTT
TGGTAGTGACTTTTCTATGGATGAAAAGGCAAAACCAAATACAATTCAAGCCGTAGTAATAGATCCAAGCACGATTGCAA
GACAAGCATCTCAAATTCGTCAAGAGAGAGAAAAAGCTTCTCGTGCAGAGACTGAGCGACTAAATCGATTAAAACAACAG
GCAGATCAATTAGAAAAAAACCGTAAAGTCGAAGAAAATCGGATTCGTAAGCTAAAAGAACAACAAGTAAAAGAGAAAAA
AGCAGCCCGTAGAGCGGAAGATGAACGTAAGAAAGCTGTCGCTCAAGCAAAAAAAGAGAAAGAACGAGCAACAGCAGCAG
AAGCCGATCGAAAGCGTAAAGTAGATACAGCAGCGAAAGCGGAAGCGGCAAGATTAACAAAAGTGAAGGCTGATAAAGAA
AAAGCAGAAAAAGCCCGTTTAGATAAAGAACGAGCAACTAAAGCAGAAGCTGAACGTGTCGCGAAAGAAAAAATAGCAAA
AGAAAAAGCAGCGAAGGCAAAGGCAAAATTAGCGGCAGAAAACGCCAAGAAAGAGAAAGCAAGAGCCGCAAAAGCGGAAG
CTGCACGTAAGCAAAAAGAAGCGGCATTAAATGACATATTTGCAGGTTTAGAAGATGAAACCGTTCAAAATAGCTCTGCG
AAACAGCAGCATATTGACGATGAAGTGAATCGCTACGCCGCAATTTTTACACAACGAATTCAACGTAACTTATTAGTCGA
TGAATCTTATGTTGGAAAAAGTTGCAAGGTAAAAATTAAACTGGCATCTTCTGGATTATTGCTCTCAGTAACAACCTTGG
GGGGTGATGCGCAGGTGTGCCGTGCTGCTAAAATAGCCGTAACTAAAGTTAATGTTTTTCAAATGCCAAAAGACGCTGAT
ATTGTAGCTAAACTTACAAATATAAACCTTACGGTTCAACCAGAATAA

Upstream 100 bases:

>100_bases
TATTCAGAGATAGTATTAGTTCTTGATGAATTAAGCCGCGCAGGGGTAAGTAAAGTTGGGTTACTTACCGATCTCAAGGA
ATAGAAGTGAATAATAATCG

Downstream 100 bases:

>100_bases
GGAAGAGCAAGTGCTTAAACGATGGATATTACCTCTTTTTATAGCGTGTTTGAGTTTTAGCCAAATAGCTAAAGCTGAAC
TAGAGCTTGTAATCACAGAG

Product: TolA protein, membrane component

Products: NA

Alternate protein names: LA Family Protein; Outer Membrane Integrity Protein lA; Protein lA; Membrane Anchored Protein In lA-TolQ-TolR Complex; LA Protein Membrane Component

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKSDNNYTFAIVVSVVLHLLLAVALIFGSDFSMDEKAKPNTIQAVVIDPSTIARQASQIRQEREKASRAETERLNRLKQQ
ADQLEKNRKVEENRIRKLKEQQVKEKKAARRAEDERKKAVAQAKKEKERATAAEADRKRKVDTAAKAEAARLTKVKADKE
KAEKARLDKERATKAEAERVAKEKIAKEKAAKAKAKLAAENAKKEKARAAKAEAARKQKEAALNDIFAGLEDETVQNSSA
KQQHIDDEVNRYAAIFTQRIQRNLLVDESYVGKSCKVKIKLASSGLLLSVTTLGGDAQVCRAAKIAVTKVNVFQMPKDAD
IVAKLTNINLTVQPE

Sequences:

>Translated_335_residues
MKSDNNYTFAIVVSVVLHLLLAVALIFGSDFSMDEKAKPNTIQAVVIDPSTIARQASQIRQEREKASRAETERLNRLKQQ
ADQLEKNRKVEENRIRKLKEQQVKEKKAARRAEDERKKAVAQAKKEKERATAAEADRKRKVDTAAKAEAARLTKVKADKE
KAEKARLDKERATKAEAERVAKEKIAKEKAAKAKAKLAAENAKKEKARAAKAEAARKQKEAALNDIFAGLEDETVQNSSA
KQQHIDDEVNRYAAIFTQRIQRNLLVDESYVGKSCKVKIKLASSGLLLSVTTLGGDAQVCRAAKIAVTKVNVFQMPKDAD
IVAKLTNINLTVQPE
>Mature_335_residues
MKSDNNYTFAIVVSVVLHLLLAVALIFGSDFSMDEKAKPNTIQAVVIDPSTIARQASQIRQEREKASRAETERLNRLKQQ
ADQLEKNRKVEENRIRKLKEQQVKEKKAARRAEDERKKAVAQAKKEKERATAAEADRKRKVDTAAKAEAARLTKVKADKE
KAEKARLDKERATKAEAERVAKEKIAKEKAAKAKAKLAAENAKKEKARAAKAEAARKQKEAALNDIFAGLEDETVQNSSA
KQQHIDDEVNRYAAIFTQRIQRNLLVDESYVGKSCKVKIKLASSGLLLSVTTLGGDAQVCRAAKIAVTKVNVFQMPKDAD
IVAKLTNINLTVQPE

Specific function: Involved In The Tonb-Independent Uptake Of Group A Colicins (Colicins A, E1, E2, E3, And K). Necessary For The Colicins To Reach Their Respective Targets After Initial Binding To The Bacteria. Also Involved In The Translocation Of Bacteriophage DNA. [C]

COG id: COG3064

COG function: function code M; Membrane protein involved in colicin uptake

Gene ontology:

Cell location: Type II Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 37237; Mature: 37237

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSDNNYTFAIVVSVVLHLLLAVALIFGSDFSMDEKAKPNTIQAVVIDPSTIARQASQIR
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHH
QEREKASRAETERLNRLKQQADQLEKNRKVEENRIRKLKEQQVKEKKAARRAEDERKKAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQAKKEKERATAAEADRKRKVDTAAKAEAARLTKVKADKEKAEKARLDKERATKAEAERV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKEKIAKEKAAKAKAKLAAENAKKEKARAAKAEAARKQKEAALNDIFAGLEDETVQNSSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCH
KQQHIDDEVNRYAAIFTQRIQRNLLVDESYVGKSCKVKIKLASSGLLLSVTTLGGDAQVC
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEEECCCEEEEEEECCCCHHHH
RAAKIAVTKVNVFQMPKDADIVAKLTNINLTVQPE
HHHHHHEEEEHHEECCCCCHHHEEEECCEEEEECC
>Mature Secondary Structure
MKSDNNYTFAIVVSVVLHLLLAVALIFGSDFSMDEKAKPNTIQAVVIDPSTIARQASQIR
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHH
QEREKASRAETERLNRLKQQADQLEKNRKVEENRIRKLKEQQVKEKKAARRAEDERKKAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQAKKEKERATAAEADRKRKVDTAAKAEAARLTKVKADKEKAEKARLDKERATKAEAERV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKEKIAKEKAAKAKAKLAAENAKKEKARAAKAEAARKQKEAALNDIFAGLEDETVQNSSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCH
KQQHIDDEVNRYAAIFTQRIQRNLLVDESYVGKSCKVKIKLASSGLLLSVTTLGGDAQVC
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEEECCCEEEEEEECCCCHHHH
RAAKIAVTKVNVFQMPKDADIVAKLTNINLTVQPE
HHHHHHEEEEHHEECCCCCHHHEEEECCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA