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The map label for this gene is nrdB [H]

Identifier: 209695249

GI number: 209695249

Start: 1886055

End: 1887188

Strand: Direct

Name: nrdB [H]

Synonym: VSAL_I1770

Alternate gene names: 209695249

Gene position: 1886055-1887188 (Clockwise)

Preceding gene: 209695248

Following gene: 209695250

Centisome position: 56.72

GC content: 37.21

Gene sequence:

>1134_bases
ATGGCGTACAGTACTTTTCGACAAGAAAAAAACGATCAATTAAAAGAACCAATGTTTTTGGGTAATTCTGTAAACGTTGC
TCGATATGATCAACAAAAATTTCAAGTATTTGAAAAACTGATCGAAAAGCAACTTTCCTTTTTCTGGCGTCCTGAAGAAG
TTGATGTTTCTGGTGATCGCATTGATTACAATAATTTACCTGAACATGAACAACATATATTCATCAGTAACCTTAAATAC
CAAACACTGCTTGATTCAATTCAAGGTCGTAGCCCTAATGTTGCCCTACTTCCTTTAGTTTCTCTTCCTGAACTAGAAAC
ATGGATCGAAACATGGTCTTTTTCTGAAACGATTCACTCACGTTCATACACACATATTGTTCGTAATATAGTGAATGACC
CAGCCATTATCTTTGATGATATTGTTGAAAATGAAGAAATCCTAAAGCGCGCAAAAGACATTGCTCATTATTACGATAAC
CTGATTCAAGCCACTAACGATTACCACCGCTTTGGCGAAGGCACTCATACTGTCAACGGTGAAGAAATTATCGTTTCAAA
ACGTGAATTAAAGAAAACGTTGTATCTTTGCTTAATGTCGGTAAATGCACTCGAAGCCATTCGTTTCTACGTAAGTTTTG
CTTGTTCTTTTGCTTTTGCAGAACGCAAATTAATGGAAGGAAATGCGAAAATAATCAAACTGATTGCTCGTGATGAAGCC
CTTCATCTAACCAGTACTCAGCATATGGTAAATCTACTTCGTACTGGTCAAGATGATCCTGAATTTGTAGAAATCGCGGC
AGAATGTGAACAAGACTGTTTCGACTTATTTAAAGCCGCGGCAGAGCAAGAAAAAGAATGGGCTAATTACCTATTTAAAG
ACGGCTCTATGATTGGTTTAAACAAGGATATTCTATGCCAATATGTAGAATATATTACAAATTTACGTATGAAGGCTGTT
GGACTATCAGGCGCTTATGATGGTGCAACTCAGAATCCTATTCCTTGGATTAACTCATGGTTAAGCTCTGATAACGTTCA
GGTTGCCCCACAAGAAACAGAAATAAGTTCATACCTTGTTGGACAAATCGATAATGACGTTAGTGCCGACGATTTAGGGG
ATTTCGAACTCTAA

Upstream 100 bases:

>100_bases
CCTATCAATACAAACCTATGTATTGATAGGCATTGCTATTATAATAGTAAGAAACAGAAAGCCCTTGCATTCTTTTGGCT
TTCATAAGATTGAGGTTTAC

Downstream 100 bases:

>100_bases
TTTGAGATCTTTCATCTGCATATTAGCCGAGTTAATTAACTCGGCTTATTTACAACAATGATAAAGCGCACAAAAATCAT
ACTAACCCCGCCTAAAAATG

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Protein B2; Protein R2; Ribonucleotide reductase 1 [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MAYSTFRQEKNDQLKEPMFLGNSVNVARYDQQKFQVFEKLIEKQLSFFWRPEEVDVSGDRIDYNNLPEHEQHIFISNLKY
QTLLDSIQGRSPNVALLPLVSLPELETWIETWSFSETIHSRSYTHIVRNIVNDPAIIFDDIVENEEILKRAKDIAHYYDN
LIQATNDYHRFGEGTHTVNGEEIIVSKRELKKTLYLCLMSVNALEAIRFYVSFACSFAFAERKLMEGNAKIIKLIARDEA
LHLTSTQHMVNLLRTGQDDPEFVEIAAECEQDCFDLFKAAAEQEKEWANYLFKDGSMIGLNKDILCQYVEYITNLRMKAV
GLSGAYDGATQNPIPWINSWLSSDNVQVAPQETEISSYLVGQIDNDVSADDLGDFEL

Sequences:

>Translated_377_residues
MAYSTFRQEKNDQLKEPMFLGNSVNVARYDQQKFQVFEKLIEKQLSFFWRPEEVDVSGDRIDYNNLPEHEQHIFISNLKY
QTLLDSIQGRSPNVALLPLVSLPELETWIETWSFSETIHSRSYTHIVRNIVNDPAIIFDDIVENEEILKRAKDIAHYYDN
LIQATNDYHRFGEGTHTVNGEEIIVSKRELKKTLYLCLMSVNALEAIRFYVSFACSFAFAERKLMEGNAKIIKLIARDEA
LHLTSTQHMVNLLRTGQDDPEFVEIAAECEQDCFDLFKAAAEQEKEWANYLFKDGSMIGLNKDILCQYVEYITNLRMKAV
GLSGAYDGATQNPIPWINSWLSSDNVQVAPQETEISSYLVGQIDNDVSADDLGDFEL
>Mature_376_residues
AYSTFRQEKNDQLKEPMFLGNSVNVARYDQQKFQVFEKLIEKQLSFFWRPEEVDVSGDRIDYNNLPEHEQHIFISNLKYQ
TLLDSIQGRSPNVALLPLVSLPELETWIETWSFSETIHSRSYTHIVRNIVNDPAIIFDDIVENEEILKRAKDIAHYYDNL
IQATNDYHRFGEGTHTVNGEEIIVSKRELKKTLYLCLMSVNALEAIRFYVSFACSFAFAERKLMEGNAKIIKLIARDEAL
HLTSTQHMVNLLRTGQDDPEFVEIAAECEQDCFDLFKAAAEQEKEWANYLFKDGSMIGLNKDILCQYVEYITNLRMKAVG
LSGAYDGATQNPIPWINSWLSSDNVQVAPQETEISSYLVGQIDNDVSADDLGDFEL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1788567, Length=377, Percent_Identity=78.2493368700265, Blast_Score=634, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 43370; Mature: 43239

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSTFRQEKNDQLKEPMFLGNSVNVARYDQQKFQVFEKLIEKQLSFFWRPEEVDVSGDR
CCCCHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
IDYNNLPEHEQHIFISNLKYQTLLDSIQGRSPNVALLPLVSLPELETWIETWSFSETIHS
CCCCCCCCHHHEEEECCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
RSYTHIVRNIVNDPAIIFDDIVENEEILKRAKDIAHYYDNLIQATNDYHRFGEGTHTVNG
HHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCC
EEIIVSKRELKKTLYLCLMSVNALEAIRFYVSFACSFAFAERKLMEGNAKIIKLIARDEA
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCC
LHLTSTQHMVNLLRTGQDDPEFVEIAAECEQDCFDLFKAAAEQEKEWANYLFKDGSMIGL
EEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
NKDILCQYVEYITNLRMKAVGLSGAYDGATQNPIPWINSWLSSDNVQVAPQETEISSYLV
CHHHHHHHHHHHHCCHHHEEECCCCCCCCCCCCCHHHHHHHCCCCCEECCCHHHHHHHHH
GQIDNDVSADDLGDFEL
HCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AYSTFRQEKNDQLKEPMFLGNSVNVARYDQQKFQVFEKLIEKQLSFFWRPEEVDVSGDR
CCCHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
IDYNNLPEHEQHIFISNLKYQTLLDSIQGRSPNVALLPLVSLPELETWIETWSFSETIHS
CCCCCCCCHHHEEEECCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
RSYTHIVRNIVNDPAIIFDDIVENEEILKRAKDIAHYYDNLIQATNDYHRFGEGTHTVNG
HHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCC
EEIIVSKRELKKTLYLCLMSVNALEAIRFYVSFACSFAFAERKLMEGNAKIIKLIARDEA
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCC
LHLTSTQHMVNLLRTGQDDPEFVEIAAECEQDCFDLFKAAAEQEKEWANYLFKDGSMIGL
EEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
NKDILCQYVEYITNLRMKAVGLSGAYDGATQNPIPWINSWLSSDNVQVAPQETEISSYLV
CHHHHHHHHHHHHCCHHHEEECCCCCCCCCCCCCHHHHHHHCCCCCEECCCHHHHHHHHH
GQIDNDVSADDLGDFEL
HCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]