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The map label for this gene is nrdA [H]

Identifier: 209695248

GI number: 209695248

Start: 1883610

End: 1885886

Strand: Direct

Name: nrdA [H]

Synonym: VSAL_I1769

Alternate gene names: 209695248

Gene position: 1883610-1885886 (Clockwise)

Preceding gene: 209695247

Following gene: 209695249

Centisome position: 56.65

GC content: 40.23

Gene sequence:

>2277_bases
ATGACCCCACAGCTTACCGTTACTAAGCGAAATGGCACCACAGAAAAGATCAATTTAGAAAAGATCCATAAAGTGATCAC
CTGGGCTGCTGAAAATCTGGATAATGTTTCTGTTTCACAAGTTGAAATGAAATCCCACATCCAGTTTTATGAAGGCATGA
AGACATCTGACATTCATGAAACCATCATCAAAGCAGCGGCCGACCTTATCTCTGAAGAAACGCCAGATTACCAATACCTT
GCTGCTCGTTTGTCTGTTTTTCACCTTCGTAAAAAAGCGTATGGCCAATTTGAGCCACCAACGCTATTTAAGCACGTAAA
TAACCTGATCGAATTAGGTAAATACGATAAGCACATTACAGAAGATTACACGGAAGCAGAATTCGACTTGATGGATCAAA
TGATCGATCACAGCCGTGATATGGACTTCTCTTACGCTGCTGTTAAACAACTAGAAGGGAAATACTTCGTACAAAACCGC
GTAACGGGCGCGATCTACGAAAGTGCTCAATTCCTTTATATTTTAGTTGCGGCGTGCTTATTTGCTAAATACCCAAAAGA
TACTCGTCTGGATTACATTCAACGTTTCTACGACGCGACCTCTAAATTTAAAATATCGTTACCAACACCGATTATGTCTG
GTGTTCGTACGCCAACACGTCAATTTAGTTCATGCGTTCTAATTGAATGTGATGATAGCCTTGATTCAATCAATGCAACG
GCAGGCTCTATTGTACGTTACGTATCACAACGTGCAGGTATTGGCATCAATGCCGGTCGTATTCGTGCACTTGGCTCTGA
AATTCGTGACGGTGAAGCATTCCATACTGGTTGTATCCCATTCTATAAATACTTCCAAACAGCAGTAAAATGTTGTTCTC
AAGGTGGTGTTCGTGGCGGCGCTGCAACCTTGTTTTACCCTATTTGGCACCGCGAAGTAGAATCACTATTGGTTCTTAAA
AATAACCGTGGCGTTGAAGAAAACCGTGTTCGTCATATGGATTACGGCGTTCAAATTAACAAGCTTATGTATACTCGCCT
AATTAAAGGGGAACACATCAGCTTATTTTCACCTTCTGATGTTCCTGGTATGTACGAGGCTTTCTTTGCTGACCAAGTAG
AGTTCGAGCGCCTGTATCTTAAATACGAACAAGACACATCAATTAAGCGTGAAACAATTAAAGCGATTGAGCTGTTCTCA
TTACTAATGCAAGAACGTGCTTCAACAGGCCGTATCTACATTCAAAACGTCGATCACTGTAATACACACAGCCCATTCGA
CCCAAGTGTTGCTCCAATTCGCCAATCTAACTTATGTTTAGAAATTGCTCTACCAACTAAGCCACTAACACACGTTAATG
ACGAAGAAGGTGAAATTGCATTATGTACTCTGTCTGCATTTAACCTAGGTGCGATAGAGAAATTAGAAGAGCTTGAAGAA
TTAGCGGAATTAGCGGTACGTGCTCTTGATGCACTTCTTGATTACCAAGACTACCCATTACCAGCAGCTTACAAATCAAC
AATGAACCGTCGTACGTTAGGTGTAGGAGTTATTAACTACGCTTATTACCTAGCGAAAAATGGCGTTAAGTACTCTGATG
GCAGTGCAAACGGATTAACGCATAAAACGTTTGAAGCAATTCAATTCTATCTTCTAAAAGCATCTGTAAATTTAGCGAAA
GAACAAGGCGCTTGCCCTGCTTTCAATGAAACTACCTATGCTCAAGGTATCCTACCTATCGATTCTTACAAAAAAGACAT
CGATAATATATGTAGTGAAGAATTACATTTAGATTGGGAAGAATTACGTTCAGACATTAAAACTCACGGTTTACGCAACT
CAACGTTATCGGCATTGATGCCGTCTGAAACCTCTTCACAAATCTCAAATGCAACGAATGGCATTGAACCACCACGTGGT
TTTGTATCAATCAAAGCATCAAAAGATGGTATTTTAAAACAAGTTGTTCCTGATTATTTGAACTTAAAAGATAATTATGA
GCTACTTTGGAACATCGGCTCTAACGATGGCTACTTGCAATTAGTCGGTATCATGCAGAAATTTGTCGACCAAGCAATTT
CAGCGAATACAAACTATGATCCAAACAGTCAGCCAAATGGCAAAGTACCAATGAAGCTATTGCTTAAAGACTTGTTAACA
GCTTATAAGCTAGGTCTAAAAACATTGTACTACCATAACACTCGTGATGGCGCGAGCGATTCACAAGGCGATTTAGCTCA
AGATGATGATTGTGAAGGCGGCGGCTGTAAAATATAA

Upstream 100 bases:

>100_bases
TAATTTTCACTAAAATAGAGTTTGATCACAAAACAACAACGAAAATTACAAAAATTACATATTTCGTTGTTTATTCTTCA
TTTTAAGGAAAAGTAGACAA

Downstream 100 bases:

>100_bases
ATAGCCCCTTCGATATTTAGCCCGCTTTCATTGCGGGCTTATTCATTTTTTAATCATTCATATCAACGCCTATCAATACA
AACCTATGTATTGATAGGCA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Protein B1; Ribonucleoside-diphosphate reductase 1 R1 subunit; Ribonucleotide reductase 1 [H]

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MTPQLTVTKRNGTTEKINLEKIHKVITWAAENLDNVSVSQVEMKSHIQFYEGMKTSDIHETIIKAAADLISEETPDYQYL
AARLSVFHLRKKAYGQFEPPTLFKHVNNLIELGKYDKHITEDYTEAEFDLMDQMIDHSRDMDFSYAAVKQLEGKYFVQNR
VTGAIYESAQFLYILVAACLFAKYPKDTRLDYIQRFYDATSKFKISLPTPIMSGVRTPTRQFSSCVLIECDDSLDSINAT
AGSIVRYVSQRAGIGINAGRIRALGSEIRDGEAFHTGCIPFYKYFQTAVKCCSQGGVRGGAATLFYPIWHREVESLLVLK
NNRGVEENRVRHMDYGVQINKLMYTRLIKGEHISLFSPSDVPGMYEAFFADQVEFERLYLKYEQDTSIKRETIKAIELFS
LLMQERASTGRIYIQNVDHCNTHSPFDPSVAPIRQSNLCLEIALPTKPLTHVNDEEGEIALCTLSAFNLGAIEKLEELEE
LAELAVRALDALLDYQDYPLPAAYKSTMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLTHKTFEAIQFYLLKASVNLAK
EQGACPAFNETTYAQGILPIDSYKKDIDNICSEELHLDWEELRSDIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRG
FVSIKASKDGILKQVVPDYLNLKDNYELLWNIGSNDGYLQLVGIMQKFVDQAISANTNYDPNSQPNGKVPMKLLLKDLLT
AYKLGLKTLYYHNTRDGASDSQGDLAQDDDCEGGGCKI

Sequences:

>Translated_758_residues
MTPQLTVTKRNGTTEKINLEKIHKVITWAAENLDNVSVSQVEMKSHIQFYEGMKTSDIHETIIKAAADLISEETPDYQYL
AARLSVFHLRKKAYGQFEPPTLFKHVNNLIELGKYDKHITEDYTEAEFDLMDQMIDHSRDMDFSYAAVKQLEGKYFVQNR
VTGAIYESAQFLYILVAACLFAKYPKDTRLDYIQRFYDATSKFKISLPTPIMSGVRTPTRQFSSCVLIECDDSLDSINAT
AGSIVRYVSQRAGIGINAGRIRALGSEIRDGEAFHTGCIPFYKYFQTAVKCCSQGGVRGGAATLFYPIWHREVESLLVLK
NNRGVEENRVRHMDYGVQINKLMYTRLIKGEHISLFSPSDVPGMYEAFFADQVEFERLYLKYEQDTSIKRETIKAIELFS
LLMQERASTGRIYIQNVDHCNTHSPFDPSVAPIRQSNLCLEIALPTKPLTHVNDEEGEIALCTLSAFNLGAIEKLEELEE
LAELAVRALDALLDYQDYPLPAAYKSTMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLTHKTFEAIQFYLLKASVNLAK
EQGACPAFNETTYAQGILPIDSYKKDIDNICSEELHLDWEELRSDIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRG
FVSIKASKDGILKQVVPDYLNLKDNYELLWNIGSNDGYLQLVGIMQKFVDQAISANTNYDPNSQPNGKVPMKLLLKDLLT
AYKLGLKTLYYHNTRDGASDSQGDLAQDDDCEGGGCKI
>Mature_757_residues
TPQLTVTKRNGTTEKINLEKIHKVITWAAENLDNVSVSQVEMKSHIQFYEGMKTSDIHETIIKAAADLISEETPDYQYLA
ARLSVFHLRKKAYGQFEPPTLFKHVNNLIELGKYDKHITEDYTEAEFDLMDQMIDHSRDMDFSYAAVKQLEGKYFVQNRV
TGAIYESAQFLYILVAACLFAKYPKDTRLDYIQRFYDATSKFKISLPTPIMSGVRTPTRQFSSCVLIECDDSLDSINATA
GSIVRYVSQRAGIGINAGRIRALGSEIRDGEAFHTGCIPFYKYFQTAVKCCSQGGVRGGAATLFYPIWHREVESLLVLKN
NRGVEENRVRHMDYGVQINKLMYTRLIKGEHISLFSPSDVPGMYEAFFADQVEFERLYLKYEQDTSIKRETIKAIELFSL
LMQERASTGRIYIQNVDHCNTHSPFDPSVAPIRQSNLCLEIALPTKPLTHVNDEEGEIALCTLSAFNLGAIEKLEELEEL
AELAVRALDALLDYQDYPLPAAYKSTMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLTHKTFEAIQFYLLKASVNLAKE
QGACPAFNETTYAQGILPIDSYKKDIDNICSEELHLDWEELRSDIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRGF
VSIKASKDGILKQVVPDYLNLKDNYELLWNIGSNDGYLQLVGIMQKFVDQAISANTNYDPNSQPNGKVPMKLLLKDLLTA
YKLGLKTLYYHNTRDGASDSQGDLAQDDDCEGGGCKI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1 contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-cone domain [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=793, Percent_Identity=27.7427490542245, Blast_Score=244, Evalue=2e-64,
Organism=Escherichia coli, GI2367133, Length=761, Percent_Identity=80.5519053876478, Blast_Score=1303, Evalue=0.0,
Organism=Escherichia coli, GI1789030, Length=724, Percent_Identity=23.8950276243094, Blast_Score=154, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17555242, Length=783, Percent_Identity=26.6922094508301, Blast_Score=225, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6322123, Length=688, Percent_Identity=28.4883720930233, Blast_Score=236, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6320914, Length=690, Percent_Identity=28.9855072463768, Blast_Score=233, Evalue=6e-62,
Organism=Drosophila melanogaster, GI17352481, Length=790, Percent_Identity=28.1012658227848, Blast_Score=253, Evalue=4e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 85263; Mature: 85131

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS51161 ATP_CONE ; PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQLTVTKRNGTTEKINLEKIHKVITWAAENLDNVSVSQVEMKSHIQFYEGMKTSDIHE
CCCCEEEEECCCCCCEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
TIIKAAADLISEETPDYQYLAARLSVFHLRKKAYGQFEPPTLFKHVNNLIELGKYDKHIT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHH
EDYTEAEFDLMDQMIDHSRDMDFSYAAVKQLEGKYFVQNRVTGAIYESAQFLYILVAACL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEHHCCHHHHHHHHHHHHHHHHHHH
FAKYPKDTRLDYIQRFYDATSKFKISLPTPIMSGVRTPTRQFSSCVLIECDDSLDSINAT
HHCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCHHHCCEEEEEECCCCHHHHHH
AGSIVRYVSQRAGIGINAGRIRALGSEIRDGEAFHTGCIPFYKYFQTAVKCCSQGGVRGG
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
AATLFYPIWHREVESLLVLKNNRGVEENRVRHMDYGVQINKLMYTRLIKGEHISLFSPSD
CHHEEHHHHHHHHHHEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCC
VPGMYEAFFADQVEFERLYLKYEQDTSIKRETIKAIELFSLLMQERASTGRIYIQNVDHC
CCHHHHHHHHHHHHEEHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDPSVAPIRQSNLCLEIALPTKPLTHVNDEEGEIALCTLSAFNLGAIEKLEELEE
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
LAELAVRALDALLDYQDYPLPAAYKSTMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLT
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCC
HKTFEAIQFYLLKASVNLAKEQGACPAFNETTYAQGILPIDSYKKDIDNICSEELHLDWE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCHH
ELRSDIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRGFVSIKASKDGILKQVVPDYL
HHHHHHHHCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
NLKDNYELLWNIGSNDGYLQLVGIMQKFVDQAISANTNYDPNSQPNGKVPMKLLLKDLLT
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
AYKLGLKTLYYHNTRDGASDSQGDLAQDDDCEGGGCKI
HHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPQLTVTKRNGTTEKINLEKIHKVITWAAENLDNVSVSQVEMKSHIQFYEGMKTSDIHE
CCCEEEEECCCCCCEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
TIIKAAADLISEETPDYQYLAARLSVFHLRKKAYGQFEPPTLFKHVNNLIELGKYDKHIT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHH
EDYTEAEFDLMDQMIDHSRDMDFSYAAVKQLEGKYFVQNRVTGAIYESAQFLYILVAACL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEHHCCHHHHHHHHHHHHHHHHHHH
FAKYPKDTRLDYIQRFYDATSKFKISLPTPIMSGVRTPTRQFSSCVLIECDDSLDSINAT
HHCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCHHHCCEEEEEECCCCHHHHHH
AGSIVRYVSQRAGIGINAGRIRALGSEIRDGEAFHTGCIPFYKYFQTAVKCCSQGGVRGG
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
AATLFYPIWHREVESLLVLKNNRGVEENRVRHMDYGVQINKLMYTRLIKGEHISLFSPSD
CHHEEHHHHHHHHHHEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCC
VPGMYEAFFADQVEFERLYLKYEQDTSIKRETIKAIELFSLLMQERASTGRIYIQNVDHC
CCHHHHHHHHHHHHEEHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDPSVAPIRQSNLCLEIALPTKPLTHVNDEEGEIALCTLSAFNLGAIEKLEELEE
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
LAELAVRALDALLDYQDYPLPAAYKSTMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLT
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCC
HKTFEAIQFYLLKASVNLAKEQGACPAFNETTYAQGILPIDSYKKDIDNICSEELHLDWE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCHH
ELRSDIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRGFVSIKASKDGILKQVVPDYL
HHHHHHHHCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
NLKDNYELLWNIGSNDGYLQLVGIMQKFVDQAISANTNYDPNSQPNGKVPMKLLLKDLLT
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
AYKLGLKTLYYHNTRDGASDSQGDLAQDDDCEGGGCKI
HHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6087316; 3287341; 9205837; 9278503; 3894026; 2663852; 8702814; 8052308; 9309223; 10493864 [H]