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The map label for this gene is rlmI

Identifier: 209695199

GI number: 209695199

Start: 1806799

End: 1807989

Strand: Direct

Name: rlmI

Synonym: VSAL_I1709

Alternate gene names: 209695199

Gene position: 1806799-1807989 (Clockwise)

Preceding gene: 209695198

Following gene: 209695200

Centisome position: 54.34

GC content: 40.22

Gene sequence:

>1191_bases
ATGACTGTTTCAATTTACCTTGTAAAAGGACGTGACAAAGCATTACGTCGTCGTCACCCTTGGATTTTCTCTCGCGGCAT
TGAAAAAATGGACGGCAAACCACAGCTTGGTGAAACAGTAGAAATCTATTCAAACAGCGGTGAGTGGCTTGCTCGTGGTG
CTTTTTCTCCTCAATCTCAAATCCGTGCCCGTGTTTGGACTTTTGACAAAAATGAAGCAATTGATAAAGATTTCTTTGTT
CGCAAACTAAACCAAGCACAAGGTTTACGCGATATTCTTGCTGAGCGTGAAGGGTTAACCGGTTATCGTTTAATCGCGGC
TGAATCAGATGGCCTACCAGGTATTACGATTGACCGTTACCAAAACTATTTTGTTTGCCAATTATTAAGTGCTGGCGCAG
AAGCAAATAAAGACCAATTAGTTGAAGCCTTAATTGAATGTTACCCAGACTGTAATATTTACGAGCGTTCTGACGTTTCT
GTTCGTAAAAAAGAAGGTCTAAAACAACGCACTGGCGTTCTTCACGGTGAAGAGCCACCTGAATCTGTCATCATTGAAGA
GAATGGCGTAAAAATTAGCGTTGATATCGTTAATGGTCATAAAACAGGCTTCTATCTTGATCAGCGTGATAGCCGTGAGC
GTTCGTGCAAATACGTAAAAGATAAATCAGTACTGAACTGTTTCTCTTATACTGGTGGTTTTGGTCTATACGCCCTAAAA
GGTGGAGCAAAACACGTTATCAATGCTGATGTATCTCAACTGGCATTAGACAACGCGAAAAACAATACTGAAATTAATGG
ATTTGACTTATCTAAAGCGGAATTTTTAAACGCTGATGTGTTTAAATTGCTTCGTGAATACCGTGATAACGGCACTAAGT
TTGATGTTGTTATTATGGATCCACCAAAATTTGCTGAATCTAAAAATCAGTTAGTTGGTGCATGTCGTGGTTATAAAGAC
ATCAACATGCTTGCGATGCAAATTTTGAACCCAGGCGGCACGCTATTAACGTACTCTTGCTCTGGTTTAATGGACACAGG
TTTGTTCCAAAAAATTGTTGCTGATGCGGCTCTTGATGCAAAACGTACGGTTCAATTTATTGAACGTTTTGAACAAGCAG
CGGATCACCCACTTGATTCTGCTTACCCTGAAGGTTTCTACCTAAAAGGTTTTGCTTGTCGCGTTATATAA

Upstream 100 bases:

>100_bases
CTTTCTCTTTCGCATTATTATCTTCTTCATTCTTTCTTATTTGCTTTTCCTTGTGACTTCAAAGAGAATAGCCCCCTAAT
TAACTCATCAGAGCATCAAT

Downstream 100 bases:

>100_bases
GCAATTAGCTTAAACCGGACATATAAAACGAAATAAAAAAGGCGAGAAGGTTAACCCCTCTCGCCTTTTCTATTTACACG
GTAACTTAATCGACTTTAAA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTVSIYLVKGRDKALRRRHPWIFSRGIEKMDGKPQLGETVEIYSNSGEWLARGAFSPQSQIRARVWTFDKNEAIDKDFFV
RKLNQAQGLRDILAEREGLTGYRLIAAESDGLPGITIDRYQNYFVCQLLSAGAEANKDQLVEALIECYPDCNIYERSDVS
VRKKEGLKQRTGVLHGEEPPESVIIEENGVKISVDIVNGHKTGFYLDQRDSRERSCKYVKDKSVLNCFSYTGGFGLYALK
GGAKHVINADVSQLALDNAKNNTEINGFDLSKAEFLNADVFKLLREYRDNGTKFDVVIMDPPKFAESKNQLVGACRGYKD
INMLAMQILNPGGTLLTYSCSGLMDTGLFQKIVADAALDAKRTVQFIERFEQAADHPLDSAYPEGFYLKGFACRVI

Sequences:

>Translated_396_residues
MTVSIYLVKGRDKALRRRHPWIFSRGIEKMDGKPQLGETVEIYSNSGEWLARGAFSPQSQIRARVWTFDKNEAIDKDFFV
RKLNQAQGLRDILAEREGLTGYRLIAAESDGLPGITIDRYQNYFVCQLLSAGAEANKDQLVEALIECYPDCNIYERSDVS
VRKKEGLKQRTGVLHGEEPPESVIIEENGVKISVDIVNGHKTGFYLDQRDSRERSCKYVKDKSVLNCFSYTGGFGLYALK
GGAKHVINADVSQLALDNAKNNTEINGFDLSKAEFLNADVFKLLREYRDNGTKFDVVIMDPPKFAESKNQLVGACRGYKD
INMLAMQILNPGGTLLTYSCSGLMDTGLFQKIVADAALDAKRTVQFIERFEQAADHPLDSAYPEGFYLKGFACRVI
>Mature_395_residues
TVSIYLVKGRDKALRRRHPWIFSRGIEKMDGKPQLGETVEIYSNSGEWLARGAFSPQSQIRARVWTFDKNEAIDKDFFVR
KLNQAQGLRDILAEREGLTGYRLIAAESDGLPGITIDRYQNYFVCQLLSAGAEANKDQLVEALIECYPDCNIYERSDVSV
RKKEGLKQRTGVLHGEEPPESVIIEENGVKISVDIVNGHKTGFYLDQRDSRERSCKYVKDKSVLNCFSYTGGFGLYALKG
GAKHVINADVSQLALDNAKNNTEINGFDLSKAEFLNADVFKLLREYRDNGTKFDVVIMDPPKFAESKNQLVGACRGYKDI
NMLAMQILNPGGTLLTYSCSGLMDTGLFQKIVADAALDAKRTVQFIERFEQAADHPLDSAYPEGFYLKGFACRVI

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Escherichia coli, GI145693111, Length=396, Percent_Identity=64.3939393939394, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1787181, Length=248, Percent_Identity=27.0161290322581, Blast_Score=85, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMI_ALISL (B6EMK8)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263128.1
- ProteinModelPortal:   B6EMK8
- GeneID:   6987318
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I1709
- HOGENOM:   HBG411897
- OMA:   RAAYSPH
- ProtClustDB:   CLSK2464030
- GO:   GO:0005737
- HAMAP:   MF_01857
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614
- SMART:   SM00359

Pfam domain/function: PF10672 Methyltrans_SAM; SSF88697 PUA-like

EC number: =2.1.1.191

Molecular weight: Translated: 44424; Mature: 44293

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSIYLVKGRDKALRRRHPWIFSRGIEKMDGKPQLGETVEIYSNSGEWLARGAFSPQSQ
CEEEEEEEECCCHHHHHCCCCHHHCCHHHCCCCCCCCCEEEEECCCCCEEEECCCCCHHH
IRARVWTFDKNEAIDKDFFVRKLNQAQGLRDILAEREGLTGYRLIAAESDGLPGITIDRY
HEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCCCCCCEEHHHH
QNYFVCQLLSAGAEANKDQLVEALIECYPDCNIYERSDVSVRKKEGLKQRTGVLHGEEPP
CCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCHHHHCHHHHHCCCCCCCCC
ESVIIEENGVKISVDIVNGHKTGFYLDQRDSRERSCKYVKDKSVLNCFSYTGGFGLYALK
CEEEEECCCCEEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
GGAKHVINADVSQLALDNAKNNTEINGFDLSKAEFLNADVFKLLREYRDNGTKFDVVIMD
CCCCEEECCCHHHHHHCCCCCCCCCCCEECCHHHHCCHHHHHHHHHHHCCCCEEEEEEEC
PPKFAESKNQLVGACRGYKDINMLAMQILNPGGTLLTYSCSGLMDTGLFQKIVADAALDA
CCCHHCCHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
KRTVQFIERFEQAADHPLDSAYPEGFYLKGFACRVI
HHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEEECC
>Mature Secondary Structure 
TVSIYLVKGRDKALRRRHPWIFSRGIEKMDGKPQLGETVEIYSNSGEWLARGAFSPQSQ
EEEEEEEECCCHHHHHCCCCHHHCCHHHCCCCCCCCCEEEEECCCCCEEEECCCCCHHH
IRARVWTFDKNEAIDKDFFVRKLNQAQGLRDILAEREGLTGYRLIAAESDGLPGITIDRY
HEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCCCCCCEEHHHH
QNYFVCQLLSAGAEANKDQLVEALIECYPDCNIYERSDVSVRKKEGLKQRTGVLHGEEPP
CCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCHHHHCHHHHHCCCCCCCCC
ESVIIEENGVKISVDIVNGHKTGFYLDQRDSRERSCKYVKDKSVLNCFSYTGGFGLYALK
CEEEEECCCCEEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
GGAKHVINADVSQLALDNAKNNTEINGFDLSKAEFLNADVFKLLREYRDNGTKFDVVIMD
CCCCEEECCCHHHHHHCCCCCCCCCCCEECCHHHHCCHHHHHHHHHHHCCCCEEEEEEEC
PPKFAESKNQLVGACRGYKDINMLAMQILNPGGTLLTYSCSGLMDTGLFQKIVADAALDA
CCCHHCCHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
KRTVQFIERFEQAADHPLDSAYPEGFYLKGFACRVI
HHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA