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The map label for this gene is potD [H]

Identifier: 209695087

GI number: 209695087

Start: 1694251

End: 1695294

Strand: Direct

Name: potD [H]

Synonym: VSAL_I1584

Alternate gene names: 209695087

Gene position: 1694251-1695294 (Clockwise)

Preceding gene: 209695086

Following gene: 209695089

Centisome position: 50.95

GC content: 39.18

Gene sequence:

>1044_bases
ATGAAAAAGTGGACAACTCTCGTCTCTACAGGACTGTGCGCTGCAGCCTTAATCGCTGGTAATGCACAAGCTGCAGATAA
AGAGCTCTATTTTTATAACTGGTCAGAATACATTCCTAACGAAATTTTAGAAGACTTTACAAAAGAAACTGGTATTAAAG
TCTATTATTCAACTTATGAGTCTAATGAAAGCATGTACGCTAAATTAAAAACTCAGGGTTCTGGATATGATCTAGTCGTT
CCTTCTACTTACTTTGTGTCTAAAATGCGCAAAGAAGGCATGCTACAAAAAATTGATAAAACAAAACTGCCGCATTTTAA
AGATTTAGACCCAAATTACTTAAATAAACCATTTGATCCAAACAATAACTATTCTATCCCATACATTTGGGGAGCGACGG
GTATTGGCGTCAACGCTGATATGATGAACCCTGCAGATCTTCATTCTTGGAATGATTTCTGGGATGAGAAATGGGAAGGT
CAGTTAATGATGATGGATGACTCTCGTGAAGTGTTCCATATCGCATTAACTAAACTTGGTTATTCAGGAAATACAACAAA
CCCAGATGAAATTAAAGCGGCTTATGAAGAGCTTAAAAAACTAATGCCAAATGTATTAGTCTTTAACTCTGATTTCCCAG
CAAATCCATACCTTGCTGGTGAAACAAGCGTAGGTATGCTTTGGAATGGCTCAGCTTATATGGCTCGTGAAGAAGGGGCA
AACATTCAGATCGTATGGCCAGATAAAGGCACGATTTTCTGGATGGACAGCTTAGCTATCCCATCAGGAGCTAAAAACAT
CGACGCGGCACACCAAATGATGGATTTCTTACTTCGTCCAGAGAACGCAGCTAAAATTGCTTTAGAGATTGGTTACCCTA
CGCCAGTTAAATCAGCATATCCTCTTCTTCCTAAATCGTTTGCAAACGATCCAAACATCTTTCCACCACAAGAAGTGATG
GACTCAGGTGAATGGCAAGATGAAGTCGGTAAAGCAAGCGCACTTTACGATGAGTATTTCCAACGCCTAAAAGTTGATAT
GTAA

Upstream 100 bases:

>100_bases
GTAAATACATTTCCATTTCTTATAAAGCCATGATGTTCATCGCAAAAATTGCTATACTGTTTAGCTATTAGTAACACTCC
CCATCTTATAGGAGAAGGAA

Downstream 100 bases:

>100_bases
TAGTTCATGTATCATTAAAACAAAAAGCCAATGATTATATTCATTGGCTTTTTTATTTAATAAGAGAAAATAACCGACAA
AGTAAACGTTATACTTCTAA

Product: spermidine/putrescine-binding periplasmic protein precursor (SPBP)

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKKWTTLVSTGLCAAALIAGNAQAADKELYFYNWSEYIPNEILEDFTKETGIKVYYSTYESNESMYAKLKTQGSGYDLVV
PSTYFVSKMRKEGMLQKIDKTKLPHFKDLDPNYLNKPFDPNNNYSIPYIWGATGIGVNADMMNPADLHSWNDFWDEKWEG
QLMMMDDSREVFHIALTKLGYSGNTTNPDEIKAAYEELKKLMPNVLVFNSDFPANPYLAGETSVGMLWNGSAYMAREEGA
NIQIVWPDKGTIFWMDSLAIPSGAKNIDAAHQMMDFLLRPENAAKIALEIGYPTPVKSAYPLLPKSFANDPNIFPPQEVM
DSGEWQDEVGKASALYDEYFQRLKVDM

Sequences:

>Translated_347_residues
MKKWTTLVSTGLCAAALIAGNAQAADKELYFYNWSEYIPNEILEDFTKETGIKVYYSTYESNESMYAKLKTQGSGYDLVV
PSTYFVSKMRKEGMLQKIDKTKLPHFKDLDPNYLNKPFDPNNNYSIPYIWGATGIGVNADMMNPADLHSWNDFWDEKWEG
QLMMMDDSREVFHIALTKLGYSGNTTNPDEIKAAYEELKKLMPNVLVFNSDFPANPYLAGETSVGMLWNGSAYMAREEGA
NIQIVWPDKGTIFWMDSLAIPSGAKNIDAAHQMMDFLLRPENAAKIALEIGYPTPVKSAYPLLPKSFANDPNIFPPQEVM
DSGEWQDEVGKASALYDEYFQRLKVDM
>Mature_347_residues
MKKWTTLVSTGLCAAALIAGNAQAADKELYFYNWSEYIPNEILEDFTKETGIKVYYSTYESNESMYAKLKTQGSGYDLVV
PSTYFVSKMRKEGMLQKIDKTKLPHFKDLDPNYLNKPFDPNNNYSIPYIWGATGIGVNADMMNPADLHSWNDFWDEKWEG
QLMMMDDSREVFHIALTKLGYSGNTTNPDEIKAAYEELKKLMPNVLVFNSDFPANPYLAGETSVGMLWNGSAYMAREEGA
NIQIVWPDKGTIFWMDSLAIPSGAKNIDAAHQMMDFLLRPENAAKIALEIGYPTPVKSAYPLLPKSFANDPNIFPPQEVM
DSGEWQDEVGKASALYDEYFQRLKVDM

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=346, Percent_Identity=59.8265895953757, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1787078, Length=340, Percent_Identity=36.1764705882353, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39315; Mature: 39315

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWTTLVSTGLCAAALIAGNAQAADKELYFYNWSEYIPNEILEDFTKETGIKVYYSTYE
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHCHHHHHHHHHHHCCCEEEEEEEC
SNESMYAKLKTQGSGYDLVVPSTYFVSKMRKEGMLQKIDKTKLPHFKDLDPNYLNKPFDP
CCCEEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCC
NNNYSIPYIWGATGIGVNADMMNPADLHSWNDFWDEKWEGQLMMMDDSREVFHIALTKLG
CCCCCCEEEECCCCCCCCCCCCCCHHCCCCHHHHCCCCCCEEEEEECCCCEEEEEEHHHC
YSGNTTNPDEIKAAYEELKKLMPNVLVFNSDFPANPYLAGETSVGMLWNGSAYMAREEGA
CCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEECCCCCEEEEECCCEEEEECCCC
NIQIVWPDKGTIFWMDSLAIPSGAKNIDAAHQMMDFLLRPENAAKIALEIGYPTPVKSAY
EEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
PLLPKSFANDPNIFPPQEVMDSGEWQDEVGKASALYDEYFQRLKVDM
CCCCHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKWTTLVSTGLCAAALIAGNAQAADKELYFYNWSEYIPNEILEDFTKETGIKVYYSTYE
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHCHHHHHHHHHHHCCCEEEEEEEC
SNESMYAKLKTQGSGYDLVVPSTYFVSKMRKEGMLQKIDKTKLPHFKDLDPNYLNKPFDP
CCCEEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCC
NNNYSIPYIWGATGIGVNADMMNPADLHSWNDFWDEKWEGQLMMMDDSREVFHIALTKLG
CCCCCCEEEECCCCCCCCCCCCCCHHCCCCHHHHCCCCCCEEEEEECCCCEEEEEEHHHC
YSGNTTNPDEIKAAYEELKKLMPNVLVFNSDFPANPYLAGETSVGMLWNGSAYMAREEGA
CCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEECCCCCEEEEECCCEEEEECCCC
NIQIVWPDKGTIFWMDSLAIPSGAKNIDAAHQMMDFLLRPENAAKIALEIGYPTPVKSAY
EEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
PLLPKSFANDPNIFPPQEVMDSGEWQDEVGKASALYDEYFQRLKVDM
CCCCHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]