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The map label for this gene is potD [H]

Identifier: 209695086

GI number: 209695086

Start: 1692980

End: 1694068

Strand: Direct

Name: potD [H]

Synonym: VSAL_I1583

Alternate gene names: 209695086

Gene position: 1692980-1694068 (Clockwise)

Preceding gene: 209695085

Following gene: 209695087

Centisome position: 50.91

GC content: 38.48

Gene sequence:

>1089_bases
ATGCCTTTTGTTCGACATGTCTTCTTTTTTATCACTCTAATGGAGAGTCAATCAATGAACAAAATGGCTGCGTTTTTTAC
TGGAACCGCCTGCGCTTTAGCAATAACAATGAATGTTGCACACGCAAAAGAAAATGATGAATTGGTATTCATGAACTGGG
GACCATACATCAACAGTAATATTATCGAAGAATTTACTAAAGAGACTGGAATTAAGGTGATCTATTCTACCTACGAGTCT
AACGAAACCTTATATGCAAAAATGAAAGCGCATAACAAAGGTTATGATTTAGTGGTTCCATCAACGTACTTTGTCGCAAA
GATGCGTGATGAAAAAATGCTTCAACCTATTGATAAATCAAAAATATCAAATTTTGATGGTTTAGATACTAACTATTTAA
ATAAACCTTTTGACCCTCAAAACGAATACTCAATCCCACACGTAGTCGCCATTACAGGTTTAGCGGTCAATACTGACATG
TATGATCCCGCTGACTTTAATAGCTGGGCTGATTTATGGAACCCAGAATTTGAAGGCCAATTAATGTTAATGGATGATAC
TCGTGAAGTATTCCATATTGCATTACGTAAATTAGGTTACTCAGGTAACTCTACCAATCCTAAAGAAATTGATGAAGCCT
ACGCTGAGTTGCAAAAACTAATGCCAAATGTATTAGTCTTTAATTCAGATAACCCAGCCGCTCCCTATTTAGCAGGAGAA
GTTGGCCTTGGTATGCTTTGGAATGGTTCTGCCGCCGCAGCACAAGCTGAAGGATTACCAATTAAACTGATCTTCCCTAA
AGAAGGCGGTATTGGTTGGGTAGACAATTTTGCAATCCCTGCTGGCGCAAAAAATGTAGAAGCATCTCATAAAATGATAA
ACTTCTTACTTCGTCCAGACATTGCTGCCAGAATATCCACGGATACTGGTTACCTAACAGCTGTTCAAGTTTCTAATGAC
AAATTCAAAGATGTTGCCCCTCTATTTCCATCTCAAGCAGATCTAGATCGCGTCGAGTGGCAATCAGCCGTGGGTAATAT
GACGGTAAAATACGAAGAGTATTTTTTAAAACTGAAAGCCGGACAGTAG

Upstream 100 bases:

>100_bases
CTTTAGTTGTGCTATCACAAGTGCTAGCAAGAGAGAAAATAAAGTAACGTTTAATAAATTCAAATACATTGTTTCGTATA
AACAATGAAATCTGGAAGAC

Downstream 100 bases:

>100_bases
CCTAACCCTTTACTGACCAACTCAATAGCAGCGTTTATCGCTGCTATTTTTATTTTTACCATTCAAATATCCTTTCTTTT
TTGTAAATACATTTCCATTT

Product: spermidine/putrescine-binding periplasmic protein precursor (SPBP)

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MPFVRHVFFFITLMESQSMNKMAAFFTGTACALAITMNVAHAKENDELVFMNWGPYINSNIIEEFTKETGIKVIYSTYES
NETLYAKMKAHNKGYDLVVPSTYFVAKMRDEKMLQPIDKSKISNFDGLDTNYLNKPFDPQNEYSIPHVVAITGLAVNTDM
YDPADFNSWADLWNPEFEGQLMLMDDTREVFHIALRKLGYSGNSTNPKEIDEAYAELQKLMPNVLVFNSDNPAAPYLAGE
VGLGMLWNGSAAAAQAEGLPIKLIFPKEGGIGWVDNFAIPAGAKNVEASHKMINFLLRPDIAARISTDTGYLTAVQVSND
KFKDVAPLFPSQADLDRVEWQSAVGNMTVKYEEYFLKLKAGQ

Sequences:

>Translated_362_residues
MPFVRHVFFFITLMESQSMNKMAAFFTGTACALAITMNVAHAKENDELVFMNWGPYINSNIIEEFTKETGIKVIYSTYES
NETLYAKMKAHNKGYDLVVPSTYFVAKMRDEKMLQPIDKSKISNFDGLDTNYLNKPFDPQNEYSIPHVVAITGLAVNTDM
YDPADFNSWADLWNPEFEGQLMLMDDTREVFHIALRKLGYSGNSTNPKEIDEAYAELQKLMPNVLVFNSDNPAAPYLAGE
VGLGMLWNGSAAAAQAEGLPIKLIFPKEGGIGWVDNFAIPAGAKNVEASHKMINFLLRPDIAARISTDTGYLTAVQVSND
KFKDVAPLFPSQADLDRVEWQSAVGNMTVKYEEYFLKLKAGQ
>Mature_361_residues
PFVRHVFFFITLMESQSMNKMAAFFTGTACALAITMNVAHAKENDELVFMNWGPYINSNIIEEFTKETGIKVIYSTYESN
ETLYAKMKAHNKGYDLVVPSTYFVAKMRDEKMLQPIDKSKISNFDGLDTNYLNKPFDPQNEYSIPHVVAITGLAVNTDMY
DPADFNSWADLWNPEFEGQLMLMDDTREVFHIALRKLGYSGNSTNPKEIDEAYAELQKLMPNVLVFNSDNPAAPYLAGEV
GLGMLWNGSAAAAQAEGLPIKLIFPKEGGIGWVDNFAIPAGAKNVEASHKMINFLLRPDIAARISTDTGYLTAVQVSNDK
FKDVAPLFPSQADLDRVEWQSAVGNMTVKYEEYFLKLKAGQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=336, Percent_Identity=57.4404761904762, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1787078, Length=292, Percent_Identity=34.9315068493151, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40520; Mature: 40388

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFVRHVFFFITLMESQSMNKMAAFFTGTACALAITMNVAHAKENDELVFMNWGPYINSN
CCHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEEEHHCCCCCCCEEEEECCCCCCHH
IIEEFTKETGIKVIYSTYESNETLYAKMKAHNKGYDLVVPSTYFVAKMRDEKMLQPIDKS
HHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCCEEEECCCEEEEECCCHHHHCCCCHH
KISNFDGLDTNYLNKPFDPQNEYSIPHVVAITGLAVNTDMYDPADFNSWADLWNPEFEGQ
HCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCHHHHCCCCCCCE
LMLMDDTREVFHIALRKLGYSGNSTNPKEIDEAYAELQKLMPNVLVFNSDNPAAPYLAGE
EEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECC
VGLGMLWNGSAAAAQAEGLPIKLIFPKEGGIGWVDNFAIPAGAKNVEASHKMINFLLRPD
CCEEEEECCCCCCHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
IAARISTDTGYLTAVQVSNDKFKDVAPLFPSQADLDRVEWQSAVGNMTVKYEEYFLKLKA
CEEEEECCCCEEEEEEECCCCCCHHCCCCCCCCCHHHHHHHHHHCCEEEEEEEEEEEEEC
GQ
CC
>Mature Secondary Structure 
PFVRHVFFFITLMESQSMNKMAAFFTGTACALAITMNVAHAKENDELVFMNWGPYINSN
CHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEEEHHCCCCCCCEEEEECCCCCCHH
IIEEFTKETGIKVIYSTYESNETLYAKMKAHNKGYDLVVPSTYFVAKMRDEKMLQPIDKS
HHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCCEEEECCCEEEEECCCHHHHCCCCHH
KISNFDGLDTNYLNKPFDPQNEYSIPHVVAITGLAVNTDMYDPADFNSWADLWNPEFEGQ
HCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCHHHHCCCCCCCE
LMLMDDTREVFHIALRKLGYSGNSTNPKEIDEAYAELQKLMPNVLVFNSDNPAAPYLAGE
EEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECC
VGLGMLWNGSAAAAQAEGLPIKLIFPKEGGIGWVDNFAIPAGAKNVEASHKMINFLLRPD
CCEEEEECCCCCCHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
IAARISTDTGYLTAVQVSNDKFKDVAPLFPSQADLDRVEWQSAVGNMTVKYEEYFLKLKA
CEEEEECCCCEEEEEEECCCCCCHHCCCCCCCCCHHHHHHHHHHCCEEEEEEEEEEEEEC
GQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]