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The map label for this gene is rlmL [H]

Identifier: 209695054

GI number: 209695054

Start: 1660800

End: 1662956

Strand: Direct

Name: rlmL [H]

Synonym: VSAL_I1551

Alternate gene names: 209695054

Gene position: 1660800-1662956 (Clockwise)

Preceding gene: 209695053

Following gene: 209695055

Centisome position: 49.95

GC content: 39.73

Gene sequence:

>2157_bases
ATGTATAATGCGAGGCAATTTAATAACCAAAAGAATCCCATGAAAAAATACTTAGCCATCACTTCTAAAGGCCTTGAAAA
CTTACTTGCAGACGAACTAGTTGCACTTGGTGTTACTGACCCAAAATTAGTTTATGCAGGGGTTACGTTTGAAGCCCCAA
TCGAGGTTGTTTATCGTTGTTGTTTGTGGAGCCGTATTGCTTCACGTTTCATTCAAATTCTGTCTGAGTTTGATGTTCGT
GATGATATGGATTTGTATCTAGGCGCTTCTGCAATTAATTGGCCTAATTATTTCACAGCAGATAAAACGTTAGTGGTGGA
TTTTAACGGTACGAACCGTGAGATTCGAAATAGCCAATACGGTGCATTGAAAGTAAAAGATGCAATTGTTGACCGTTTCA
CAAAGGCTGATTTAGAACGTCCGAATATTGATAAAGCGGAACCTGATTTACGTGTACATATGCGTTTATCTGGCGAGAAA
GGGATTCTTGGTTTTGATTTAATCGGTTCAGGCTTACATCAGCGTGGTTACCGTACTGAAGCAGGTCGTGCACCACTTCG
TGAAACATTAGCAGCAGCACTTGTACTTCGCAGTACATGGGATGAAAGCAAACCGCTATTAGATCCAATGTGTGGTTCAG
GTACGTTACTGATTGAAGCTGCACTTATGGCGTGTGAAATGGCACCTGGTGTGAAACGTGAAAAATGGTGTTTTGAAGCG
TTGTCTGATTTTGATCCAGAAGTGTGGACTGAAATTCGTTCAGAAGCTCGAGTTAAGAGTCGCCGTGGCGTTAAGAAAGT
CGATACGCATTTTTACGGTTTTGATCGTGATTATCGTGTAATTCAAACGGCTCGCGATAATGCTCGTCGCGCTGGTGTTG
AAGATTTAATCACATTTGATGTTGGTGATGCAACTAAGATTGAACGTCCTGAGGGCTTTGAGAATGGGGTTGTAATTTGT
AACCCTCCTTATGGTGAACGTTTAAGTACTGAACCTGCACTAATTGCACTATACAGTGAATTTGGTCGCCAACTAAAAGA
ACAATTTGGTGGCTGTACTGCATCAATCTATTCAAGTAATGATGATTTGCTTGCGTGTATTCGTATGCGTGCAGACAAGC
AATTCAAACTGAACAATGGGGCGTTACCTTGTGTTCAGAAAAACTATTCAATTAGTGAATCGGCTGAACGTAAAGAAGCG
GCAAACATTGAAGTTGCTCCTGAGTTTATGAACCGTCTTAAAAAGAATTTGTCTAAACTTGGCAAATGGGCACGTAAAGA
GAAGTTAGATTGTTACCGTCTTTATGATGCCGATTTACCAGATTACAATGCGGCAATTGATGTATACAAAGATTACATCA
TCATTCAAGAATATACGGCACCAAAAGAAATTTCAGAAGATAAAGCACGTCGTCGTTTAACTGACATGATCCGCGCAACA
GTGTTAGTGACTGGTGTTGAAACCAATAACGTTATTTTGAAAGTACGTCAAAAGCAGTCAGGCAAAAACCAATATCAAAA
GTTAGCAGAAAAATCTCGTTACTTTGATGTTGAAGAGTACGGCGTAAAATTGATTGTTAATTTACAAGATTACCTAGATA
CGGGGTTATTCTTGGATCATAAATTAACTCGTAAAATGATTGGTGATATGGCCGCAGGAAAAGATTTCCTAAACCTATTT
GCTTATACCGGCTCTGCGACTGTTCATGCCGCTTGTGGTGGTGCTAAATCGACAATGACCATTGATATGTCTCGTACCTA
CCTAGAGTGGGCTCAGAAGAACATGAACACCAATGATCAAACAGGAACGCAACATCAGTTCCTACAAGCCGATTGTCTAC
AATGGCTACAACAAGCAGAAGGTGAGTTTGATTTAATCTTTATTGACCCACCAACATTCTCTAACTCTAAGCGTATGGAC
CAAACGTTTGATGTTCAACGTGACCACATTATGTTGCTTGAGAACCTAAAACGTATGTTACGTCAAGACGGTACTGTTGT
ATTTTCTAACAATAAACGTAACTTTAAAATGGATGAAGAAGCGCTAGATAAAGCGGGTCTTCAAGCTAAGAATATTTCAA
AACAAACATTGCCGTTAGATTTTGCTCGTAATAAGCATATTCATAACTGCTGGATTATTACTCACAAGGAAGACTAG

Upstream 100 bases:

>100_bases
TCCATCATCAGTGAGATTAACTTATTGTTTTTATAGTCTTTATTTGAAGAATTTGAGGATGGTTTATCTGTGATCTATTT
ATCCTATTTCCTTAGGATTC

Downstream 100 bases:

>100_bases
AGATAATGATAACTCTCTACAGCACGGAAGGCTGCCACCTATGCGAGCAAGCATTTGATTTGCTCGTTGAGGTGGGTGTA
AATATGGAACAAGTAGATAC

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MYNARQFNNQKNPMKKYLAITSKGLENLLADELVALGVTDPKLVYAGVTFEAPIEVVYRCCLWSRIASRFIQILSEFDVR
DDMDLYLGASAINWPNYFTADKTLVVDFNGTNREIRNSQYGALKVKDAIVDRFTKADLERPNIDKAEPDLRVHMRLSGEK
GILGFDLIGSGLHQRGYRTEAGRAPLRETLAAALVLRSTWDESKPLLDPMCGSGTLLIEAALMACEMAPGVKREKWCFEA
LSDFDPEVWTEIRSEARVKSRRGVKKVDTHFYGFDRDYRVIQTARDNARRAGVEDLITFDVGDATKIERPEGFENGVVIC
NPPYGERLSTEPALIALYSEFGRQLKEQFGGCTASIYSSNDDLLACIRMRADKQFKLNNGALPCVQKNYSISESAERKEA
ANIEVAPEFMNRLKKNLSKLGKWARKEKLDCYRLYDADLPDYNAAIDVYKDYIIIQEYTAPKEISEDKARRRLTDMIRAT
VLVTGVETNNVILKVRQKQSGKNQYQKLAEKSRYFDVEEYGVKLIVNLQDYLDTGLFLDHKLTRKMIGDMAAGKDFLNLF
AYTGSATVHAACGGAKSTMTIDMSRTYLEWAQKNMNTNDQTGTQHQFLQADCLQWLQQAEGEFDLIFIDPPTFSNSKRMD
QTFDVQRDHIMLLENLKRMLRQDGTVVFSNNKRNFKMDEEALDKAGLQAKNISKQTLPLDFARNKHIHNCWIITHKED

Sequences:

>Translated_718_residues
MYNARQFNNQKNPMKKYLAITSKGLENLLADELVALGVTDPKLVYAGVTFEAPIEVVYRCCLWSRIASRFIQILSEFDVR
DDMDLYLGASAINWPNYFTADKTLVVDFNGTNREIRNSQYGALKVKDAIVDRFTKADLERPNIDKAEPDLRVHMRLSGEK
GILGFDLIGSGLHQRGYRTEAGRAPLRETLAAALVLRSTWDESKPLLDPMCGSGTLLIEAALMACEMAPGVKREKWCFEA
LSDFDPEVWTEIRSEARVKSRRGVKKVDTHFYGFDRDYRVIQTARDNARRAGVEDLITFDVGDATKIERPEGFENGVVIC
NPPYGERLSTEPALIALYSEFGRQLKEQFGGCTASIYSSNDDLLACIRMRADKQFKLNNGALPCVQKNYSISESAERKEA
ANIEVAPEFMNRLKKNLSKLGKWARKEKLDCYRLYDADLPDYNAAIDVYKDYIIIQEYTAPKEISEDKARRRLTDMIRAT
VLVTGVETNNVILKVRQKQSGKNQYQKLAEKSRYFDVEEYGVKLIVNLQDYLDTGLFLDHKLTRKMIGDMAAGKDFLNLF
AYTGSATVHAACGGAKSTMTIDMSRTYLEWAQKNMNTNDQTGTQHQFLQADCLQWLQQAEGEFDLIFIDPPTFSNSKRMD
QTFDVQRDHIMLLENLKRMLRQDGTVVFSNNKRNFKMDEEALDKAGLQAKNISKQTLPLDFARNKHIHNCWIITHKED
>Mature_718_residues
MYNARQFNNQKNPMKKYLAITSKGLENLLADELVALGVTDPKLVYAGVTFEAPIEVVYRCCLWSRIASRFIQILSEFDVR
DDMDLYLGASAINWPNYFTADKTLVVDFNGTNREIRNSQYGALKVKDAIVDRFTKADLERPNIDKAEPDLRVHMRLSGEK
GILGFDLIGSGLHQRGYRTEAGRAPLRETLAAALVLRSTWDESKPLLDPMCGSGTLLIEAALMACEMAPGVKREKWCFEA
LSDFDPEVWTEIRSEARVKSRRGVKKVDTHFYGFDRDYRVIQTARDNARRAGVEDLITFDVGDATKIERPEGFENGVVIC
NPPYGERLSTEPALIALYSEFGRQLKEQFGGCTASIYSSNDDLLACIRMRADKQFKLNNGALPCVQKNYSISESAERKEA
ANIEVAPEFMNRLKKNLSKLGKWARKEKLDCYRLYDADLPDYNAAIDVYKDYIIIQEYTAPKEISEDKARRRLTDMIRAT
VLVTGVETNNVILKVRQKQSGKNQYQKLAEKSRYFDVEEYGVKLIVNLQDYLDTGLFLDHKLTRKMIGDMAAGKDFLNLF
AYTGSATVHAACGGAKSTMTIDMSRTYLEWAQKNMNTNDQTGTQHQFLQADCLQWLQQAEGEFDLIFIDPPTFSNSKRMD
QTFDVQRDHIMLLENLKRMLRQDGTVVFSNNKRNFKMDEEALDKAGLQAKNISKQTLPLDFARNKHIHNCWIITHKED

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=702, Percent_Identity=55.6980056980057, Blast_Score=825, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=245, Percent_Identity=28.1632653061224, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 81841; Mature: 81841

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNARQFNNQKNPMKKYLAITSKGLENLLADELVALGVTDPKLVYAGVTFEAPIEVVYRC
CCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHH
CLWSRIASRFIQILSEFDVRDDMDLYLGASAINWPNYFTADKTLVVDFNGTNREIRNSQY
HHHHHHHHHHHHHHHHCCCCCCCHHEECCCEECCCCEECCCCEEEEEECCCCCHHCCCCC
GALKVKDAIVDRFTKADLERPNIDKAEPDLRVHMRLSGEKGILGFDLIGSGLHQRGYRTE
CEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEHHHHCCHHHHCCCCCC
AGRAPLRETLAAALVLRSTWDESKPLLDPMCGSGTLLIEAALMACEMAPGVKREKWCFEA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
LSDFDPEVWTEIRSEARVKSRRGVKKVDTHFYGFDRDYRVIQTARDNARRAGVEDLITFD
HHCCCHHHHHHHHHHHHHHHHCCCHHHHHHEECCCCCHHHHHHHHHHHHHCCHHHEEEEC
VGDATKIERPEGFENGVVICNPPYGERLSTEPALIALYSEFGRQLKEQFGGCTASIYSSN
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCC
DDLLACIRMRADKQFKLNNGALPCVQKNYSISESAERKEAANIEVAPEFMNRLKKNLSKL
CHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEECHHHHHHHHHHHHHH
GKWARKEKLDCYRLYDADLPDYNAAIDVYKDYIIIQEYTAPKEISEDKARRRLTDMIRAT
HHHHHHCCCCEEEEECCCCCCCCHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
VLVTGVETNNVILKVRQKQSGKNQYQKLAEKSRYFDVEEYGVKLIVNLQDYLDTGLFLDH
HEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHCCEEEEEEEHHHHCCCCHHHH
KLTRKMIGDMAAGKDFLNLFAYTGSATVHAACGGAKSTMTIDMSRTYLEWAQKNMNTNDQ
HHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCEEEEECHHHHHHHHHHCCCCCCC
TGTQHQFLQADCLQWLQQAEGEFDLIFIDPPTFSNSKRMDQTFDVQRDHIMLLENLKRML
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHH
RQDGTVVFSNNKRNFKMDEEALDKAGLQAKNISKQTLPLDFARNKHIHNCWIITHKED
HCCCCEEEECCCCCCCCCHHHHHHCCCCHHCCCCCCCCCHHHCCCCEEEEEEEEECCC
>Mature Secondary Structure
MYNARQFNNQKNPMKKYLAITSKGLENLLADELVALGVTDPKLVYAGVTFEAPIEVVYRC
CCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHH
CLWSRIASRFIQILSEFDVRDDMDLYLGASAINWPNYFTADKTLVVDFNGTNREIRNSQY
HHHHHHHHHHHHHHHHCCCCCCCHHEECCCEECCCCEECCCCEEEEEECCCCCHHCCCCC
GALKVKDAIVDRFTKADLERPNIDKAEPDLRVHMRLSGEKGILGFDLIGSGLHQRGYRTE
CEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEHHHHCCHHHHCCCCCC
AGRAPLRETLAAALVLRSTWDESKPLLDPMCGSGTLLIEAALMACEMAPGVKREKWCFEA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
LSDFDPEVWTEIRSEARVKSRRGVKKVDTHFYGFDRDYRVIQTARDNARRAGVEDLITFD
HHCCCHHHHHHHHHHHHHHHHCCCHHHHHHEECCCCCHHHHHHHHHHHHHCCHHHEEEEC
VGDATKIERPEGFENGVVICNPPYGERLSTEPALIALYSEFGRQLKEQFGGCTASIYSSN
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCC
DDLLACIRMRADKQFKLNNGALPCVQKNYSISESAERKEAANIEVAPEFMNRLKKNLSKL
CHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEECHHHHHHHHHHHHHH
GKWARKEKLDCYRLYDADLPDYNAAIDVYKDYIIIQEYTAPKEISEDKARRRLTDMIRAT
HHHHHHCCCCEEEEECCCCCCCCHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
VLVTGVETNNVILKVRQKQSGKNQYQKLAEKSRYFDVEEYGVKLIVNLQDYLDTGLFLDH
HEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHCCEEEEEEEHHHHCCCCHHHH
KLTRKMIGDMAAGKDFLNLFAYTGSATVHAACGGAKSTMTIDMSRTYLEWAQKNMNTNDQ
HHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCEEEEECHHHHHHHHHHCCCCCCC
TGTQHQFLQADCLQWLQQAEGEFDLIFIDPPTFSNSKRMDQTFDVQRDHIMLLENLKRML
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHH
RQDGTVVFSNNKRNFKMDEEALDKAGLQAKNISKQTLPLDFARNKHIHNCWIITHKED
HCCCCEEEECCCCCCCCCHHHHHHCCCCHHCCCCCCCCCHHHCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA