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The map label for this gene is kefA [H]

Identifier: 209695030

GI number: 209695030

Start: 1625550

End: 1626425

Strand: Direct

Name: kefA [H]

Synonym: VSAL_I1524

Alternate gene names: 209695030

Gene position: 1625550-1626425 (Clockwise)

Preceding gene: 209695028

Following gene: 209695032

Centisome position: 48.89

GC content: 36.07

Gene sequence:

>876_bases
ATGATTCGTTTATATGCACTTTTATTCTCCCTTTTTTTAGGGCAAACCGTCTTCGCCGAAGATCTTCAATCTATCGATAA
TCTACAACAATTTGCGAGTTTAATTCGTTGGAGTGGTGTCGCTTTTTCTTTGTTTATCATTATTGGCGCTTGGTTATTTC
TTCGATTTATTCAATCGATTGTTGATGGAGTAAGTAGCCAATTTGCACAACGCCGAATGTTAATGCAAAAATTGCAATCT
TTCTTTCAATTCTTTATTTATATGTCGACGGCGGTTGCTATCTTTATGTTGAGTTTTAGGGTTGATGATCGCGTACTTGC
ACTCATTGGAGGAACGATTGCGGTATCCGTAGGTTTTGCATTAAAAGATTTAACCGCATCGTTCATTGCAGGGTTAACGG
TTATGATAGATCGTCCATTCCAAGTAGGTGACCGCGTCACTTTTGAAGGGCACTATGGAGACATTATTGCGATAGGTCTA
CGGTCAGTACGAATGCAAACACTTAATGATGATATTGTAACGATACCGAACAATAAATTCTTAAATGACGTAACTGTGAG
TGGAAATTATGGTGGGATTGACATGCAGGTTGTTATCCCTTTTTATATTGGAATGGATCAGGATATTAATAAGGCTCGAG
ATATCATACAAGAGGCTGCATCTTCAAGTCGGTATATTCACTTACCAAAACCAGTTGTTGTTTTAGTTAAACAAACGATT
ACAGAAAATTATCTGGTGATCCAACTTACGTGTAAAGCTTATGTTGTTGATATTAAATTAGAAAAATTATTCGAAACCGA
TATTACTTTACGTGTAATGCAAGAATTTAGAAAAGAAGACATTATGCCTCCAACTATAGCAATTCAAAAGAAATAG

Upstream 100 bases:

>100_bases
GGTTATATTGAATGGAACGAGAACAAGGCGCGGATATCAGATCATTGGTATCGACATATTACTAACATACTTCATCGTCA
GCATTTATTGGTAAAATAAC

Downstream 100 bases:

>100_bases
AATTGAGCTAGGATGGTTATACCAATCGTTTCTTTATGACGTTTAAATAGAAGAAGGATTTATTCAGATTTGTAACAATA
AATAAAGCCAGTAAGAGTCG

Product: mechanosensitive ion channel

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MIRLYALLFSLFLGQTVFAEDLQSIDNLQQFASLIRWSGVAFSLFIIIGAWLFLRFIQSIVDGVSSQFAQRRMLMQKLQS
FFQFFIYMSTAVAIFMLSFRVDDRVLALIGGTIAVSVGFALKDLTASFIAGLTVMIDRPFQVGDRVTFEGHYGDIIAIGL
RSVRMQTLNDDIVTIPNNKFLNDVTVSGNYGGIDMQVVIPFYIGMDQDINKARDIIQEAASSSRYIHLPKPVVVLVKQTI
TENYLVIQLTCKAYVVDIKLEKLFETDITLRVMQEFRKEDIMPPTIAIQKK

Sequences:

>Translated_291_residues
MIRLYALLFSLFLGQTVFAEDLQSIDNLQQFASLIRWSGVAFSLFIIIGAWLFLRFIQSIVDGVSSQFAQRRMLMQKLQS
FFQFFIYMSTAVAIFMLSFRVDDRVLALIGGTIAVSVGFALKDLTASFIAGLTVMIDRPFQVGDRVTFEGHYGDIIAIGL
RSVRMQTLNDDIVTIPNNKFLNDVTVSGNYGGIDMQVVIPFYIGMDQDINKARDIIQEAASSSRYIHLPKPVVVLVKQTI
TENYLVIQLTCKAYVVDIKLEKLFETDITLRVMQEFRKEDIMPPTIAIQKK
>Mature_291_residues
MIRLYALLFSLFLGQTVFAEDLQSIDNLQQFASLIRWSGVAFSLFIIIGAWLFLRFIQSIVDGVSSQFAQRRMLMQKLQS
FFQFFIYMSTAVAIFMLSFRVDDRVLALIGGTIAVSVGFALKDLTASFIAGLTVMIDRPFQVGDRVTFEGHYGDIIAIGL
RSVRMQTLNDDIVTIPNNKFLNDVTVSGNYGGIDMQVVIPFYIGMDQDINKARDIIQEAASSSRYIHLPKPVVVLVKQTI
TENYLVIQLTCKAYVVDIKLEKLFETDITLRVMQEFRKEDIMPPTIAIQKK

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=195, Percent_Identity=25.1282051282051, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 32970; Mature: 32970

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLYALLFSLFLGQTVFAEDLQSIDNLQQFASLIRWSGVAFSLFIIIGAWLFLRFIQSI
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VDGVSSQFAQRRMLMQKLQSFFQFFIYMSTAVAIFMLSFRVDDRVLALIGGTIAVSVGFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHH
LKDLTASFIAGLTVMIDRPFQVGDRVTFEGHYGDIIAIGLRSVRMQTLNDDIVTIPNNKF
HHHHHHHHHCCHHEEEECCCCCCCEEEECCCCCCEEEEHHHHHHHHHCCCCEEECCCCCE
LNDVTVSGNYGGIDMQVVIPFYIGMDQDINKARDIIQEAASSSRYIHLPKPVVVLVKQTI
ECEEEEECCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
TENYLVIQLTCKAYVVDIKLEKLFETDITLRVMQEFRKEDIMPPTIAIQKK
CCCEEEEEEEEEEEEEEEEHHHHHHCCHHHHHHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure
MIRLYALLFSLFLGQTVFAEDLQSIDNLQQFASLIRWSGVAFSLFIIIGAWLFLRFIQSI
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VDGVSSQFAQRRMLMQKLQSFFQFFIYMSTAVAIFMLSFRVDDRVLALIGGTIAVSVGFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHH
LKDLTASFIAGLTVMIDRPFQVGDRVTFEGHYGDIIAIGLRSVRMQTLNDDIVTIPNNKF
HHHHHHHHHCCHHEEEECCCCCCCEEEECCCCCCEEEEHHHHHHHHHCCCCEEECCCCCE
LNDVTVSGNYGGIDMQVVIPFYIGMDQDINKARDIIQEAASSSRYIHLPKPVVVLVKQTI
ECEEEEECCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
TENYLVIQLTCKAYVVDIKLEKLFETDITLRVMQEFRKEDIMPPTIAIQKK
CCCEEEEEEEEEEEEEEEEHHHHHHCCHHHHHHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]