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The map label for this gene is kipI [H]

Identifier: 209694956

GI number: 209694956

Start: 1551597

End: 1552316

Strand: Direct

Name: kipI [H]

Synonym: VSAL_I1437

Alternate gene names: 209694956

Gene position: 1551597-1552316 (Clockwise)

Preceding gene: 209694955

Following gene: 209694957

Centisome position: 46.66

GC content: 42.08

Gene sequence:

>720_bases
ATGAAAATATCAGCCGTTAATGAGTCCACTGTTTTAGTTGTATTTTCCGATACGGTTAGTCCTGAAACTGCCGATATTAT
TGCTCAATCCGTTGCTATGATTAATCAAGAATCGGATTTGTTCGTGATTGATATGGTGCCATCGTACACATCGATTTTAA
TTACTTATGATTTAACGCGCATAGAGTTACGCTCATTTATGTCTCGTTTACATCACTGTCTTCACCAAGATAACAACAGT
GGGCAATTACCGTTGTCTTCTGTTGTGATTGAATTACCTGTGTATTATGGACCAGAAGTGTCCCTTGATGCCGAAGACAT
CAGTCGTCATACCGGTTTATCGTTTGATGAGGTCGTGTCTATTCATTCCTCTCAAGAGTACCGCGTGTATGCAATTGGTT
TTGCTCCGGGCTTTGCTTATTTAGGCAATACTGACTCTCGCATTCATATTCCTCGTAAAAGTACGCCACGGTTGAGTGTA
CCAGCAGGCAGCTTGGCCATCGCTGAACAACAAACGGCGATTTACCCAAAATCTTCACCGGGAGGATGGCAGGTGATTGG
GAGAACTCCTATTAATTTGATTGATTTTAATCGAGACAATCTTACGGTATTTGAGATGGGGGCAAAAGTGACTTTCAGAC
CAATTTCACGGGAAGAATATTTAACGATGGGAGGGGAGCTTCCAGAGGCATTGTTAGTGAAAGAGGGAAGCAAAGTATGA

Upstream 100 bases:

>100_bases
CGATTCACGCAGATACCTTATGTATTCATGGTGAGGGCCCTTTTGCGGTATCGACAGCGACGTATTTACGTAAAAAGTTG
CAGAAAAAGGCGGAGTAATC

Downstream 100 bases:

>100_bases
CACTACGAATTCACAGCACAGGGCCATTGGCGCTACTTCAAGACAAGGGTCGATATGGTCACCAATCGGTAGGGGTGACT
CCCGGCGGACCAATGGATGA

Product: allophanate hydrolase subunit 1

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKISAVNESTVLVVFSDTVSPETADIIAQSVAMINQESDLFVIDMVPSYTSILITYDLTRIELRSFMSRLHHCLHQDNNS
GQLPLSSVVIELPVYYGPEVSLDAEDISRHTGLSFDEVVSIHSSQEYRVYAIGFAPGFAYLGNTDSRIHIPRKSTPRLSV
PAGSLAIAEQQTAIYPKSSPGGWQVIGRTPINLIDFNRDNLTVFEMGAKVTFRPISREEYLTMGGELPEALLVKEGSKV

Sequences:

>Translated_239_residues
MKISAVNESTVLVVFSDTVSPETADIIAQSVAMINQESDLFVIDMVPSYTSILITYDLTRIELRSFMSRLHHCLHQDNNS
GQLPLSSVVIELPVYYGPEVSLDAEDISRHTGLSFDEVVSIHSSQEYRVYAIGFAPGFAYLGNTDSRIHIPRKSTPRLSV
PAGSLAIAEQQTAIYPKSSPGGWQVIGRTPINLIDFNRDNLTVFEMGAKVTFRPISREEYLTMGGELPEALLVKEGSKV
>Mature_239_residues
MKISAVNESTVLVVFSDTVSPETADIIAQSVAMINQESDLFVIDMVPSYTSILITYDLTRIELRSFMSRLHHCLHQDNNS
GQLPLSSVVIELPVYYGPEVSLDAEDISRHTGLSFDEVVSIHSSQEYRVYAIGFAPGFAYLGNTDSRIHIPRKSTPRLSV
PAGSLAIAEQQTAIYPKSSPGGWQVIGRTPINLIDFNRDNLTVFEMGAKVTFRPISREEYLTMGGELPEALLVKEGSKV

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=128, Percent_Identity=46.875, Blast_Score=124, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 26380; Mature: 26380

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISAVNESTVLVVFSDTVSPETADIIAQSVAMINQESDLFVIDMVPSYTSILITYDLTR
CEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEEHHH
IELRSFMSRLHHCLHQDNNSGQLPLSSVVIELPVYYGPEVSLDAEDISRHTGLSFDEVVS
HHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEECCEECCCCEECHHHHHHHCCCCHHHHHE
IHSSQEYRVYAIGFAPGFAYLGNTDSRIHIPRKSTPRLSVPAGSLAIAEQQTAIYPKSSP
ECCCCCEEEEEEEECCCEEEECCCCCEEECCCCCCCEEECCCCCEEEECCCEEECCCCCC
GGWQVIGRTPINLIDFNRDNLTVFEMGAKVTFRPISREEYLTMGGELPEALLVKEGSKV
CCEEEEECCCCEEEEECCCCEEEEEECCEEEEEECCCCHHEECCCCCCCEEEECCCCCC
>Mature Secondary Structure
MKISAVNESTVLVVFSDTVSPETADIIAQSVAMINQESDLFVIDMVPSYTSILITYDLTR
CEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEEHHH
IELRSFMSRLHHCLHQDNNSGQLPLSSVVIELPVYYGPEVSLDAEDISRHTGLSFDEVVS
HHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEECCEECCCCEECHHHHHHHCCCCHHHHHE
IHSSQEYRVYAIGFAPGFAYLGNTDSRIHIPRKSTPRLSVPAGSLAIAEQQTAIYPKSSP
ECCCCCEEEEEEEECCCEEEECCCCCEEECCCCCCCEEECCCCCEEEECCCEEECCCCCC
GGWQVIGRTPINLIDFNRDNLTVFEMGAKVTFRPISREEYLTMGGELPEALLVKEGSKV
CCEEEEECCCCEEEEECCCCEEEEEECCEEEEEECCCCHHEECCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]