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The map label for this gene is prfA

Identifier: 209694372

GI number: 209694372

Start: 876495

End: 877583

Strand: Direct

Name: prfA

Synonym: VSAL_I0790

Alternate gene names: 209694372

Gene position: 876495-877583 (Clockwise)

Preceding gene: 209694371

Following gene: 209694373

Centisome position: 26.36

GC content: 43.25

Gene sequence:

>1089_bases
ATGAAAGCATCAATTCGAGTAAAATTGGAAACGCTGGTTGAGCGTTATGAAGAAGTACAGCATTTATTGGGGGATCCAGG
TGTTATTGGTGATCAAAATAAATTCCGTGCGTTATCTCGTGAATACTCTCAACTGGAAGAAGTAACACAATGCTTCCAAG
CGTATGAAAAAGTACAAGAAGATCTTGTTGCTGCTCAAGAAATGGCACAGGAAGATGATGCTGAAATGCGCGAAATGGCG
CAAGACGAAATTAAAGAAGCGAAAGAAGCAAGTGAACGTCTAACGGATGAATTACAAGTGCTTCTTATTCCAAAAGATCC
AAATGATGAACGTAACTGTTTCTTAGAGATCCGTGCCGGTGCTGGTGGTGATGAAGCGGGTATTTTTGCGGGGAACCTTT
TCCGTATGTATTCTCGTTTTGCTGAGAAAAAAGGGTGGCGTATTGAGGTCATGAGTTCTCATGCTTCAGAACAAGGCGGC
TTTAAAGAAATGATTGCAAAAGTAAGCGGCGATGGCGCTTATGGTATATTGAAATTTGAATCAGGTGGTCACCGAGTTCA
ACGTGTACCAGAAACTGAATCGCAAGGTCGTGTGCATACTTCAGCGTGTACTATTGCTGTAATGGCTGAAATTCCAGAAG
CGGATTTACCCGAGATTAAATCGTCTGATTTGAAAATTGATACTTTCCGTTCATCAGGAGCTGGTGGTCAGCACGTTAAC
ACCACCGATTCCGCTATTCGTATTACTCACTTACCAACGGGTACCGTTGTAGAGTGTCAAGATGAGCGTTCACAGCATAA
AAACAAAGCAAAAGCGATGTCGGTTCTTGCTGCCCGTATTATTCAAGCTGAAGAAGCTCGTCGTGCCGCAGTTGTTTCTG
ATACGCGTCGTAATCTATTAGGTTCGGGTGACCGTTCAGACCGTATTCGTACGTACAACTACCCACAAAGTCGTGTGTCT
GATCACCGTATTAACCTGACTATCTACCGTTTGAATGAAGTGATGGAAGGGGATCTAGCGGCGTTGATTGAACCTGTCGT
ATTAGAATATCAAGCTGATCAACTAGCGGCTTTAGCTGAACAAAACTAA

Upstream 100 bases:

>100_bases
CAAAAATGGTGAGCCGGACAAACTTGCTGTGATCCGTACCAGTATTGGTCTAGACAACTAAGTGTAGCTTCTGCTACCAA
CATAAAGAATCAATTATAAT

Downstream 100 bases:

>100_bases
TGAGCATTTCTATTGAAGCGCTATTAATGCGTTCAATTCAATTGCTGAAACTTGAGGGGAGTGATTCTCCTCAAGTTGAT
GCTGCTGTATTGCTGTGTCA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MKASIRVKLETLVERYEEVQHLLGDPGVIGDQNKFRALSREYSQLEEVTQCFQAYEKVQEDLVAAQEMAQEDDAEMREMA
QDEIKEAKEASERLTDELQVLLIPKDPNDERNCFLEIRAGAGGDEAGIFAGNLFRMYSRFAEKKGWRIEVMSSHASEQGG
FKEMIAKVSGDGAYGILKFESGGHRVQRVPETESQGRVHTSACTIAVMAEIPEADLPEIKSSDLKIDTFRSSGAGGQHVN
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMSVLAARIIQAEEARRAAVVSDTRRNLLGSGDRSDRIRTYNYPQSRVS
DHRINLTIYRLNEVMEGDLAALIEPVVLEYQADQLAALAEQN

Sequences:

>Translated_362_residues
MKASIRVKLETLVERYEEVQHLLGDPGVIGDQNKFRALSREYSQLEEVTQCFQAYEKVQEDLVAAQEMAQEDDAEMREMA
QDEIKEAKEASERLTDELQVLLIPKDPNDERNCFLEIRAGAGGDEAGIFAGNLFRMYSRFAEKKGWRIEVMSSHASEQGG
FKEMIAKVSGDGAYGILKFESGGHRVQRVPETESQGRVHTSACTIAVMAEIPEADLPEIKSSDLKIDTFRSSGAGGQHVN
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMSVLAARIIQAEEARRAAVVSDTRRNLLGSGDRSDRIRTYNYPQSRVS
DHRINLTIYRLNEVMEGDLAALIEPVVLEYQADQLAALAEQN
>Mature_362_residues
MKASIRVKLETLVERYEEVQHLLGDPGVIGDQNKFRALSREYSQLEEVTQCFQAYEKVQEDLVAAQEMAQEDDAEMREMA
QDEIKEAKEASERLTDELQVLLIPKDPNDERNCFLEIRAGAGGDEAGIFAGNLFRMYSRFAEKKGWRIEVMSSHASEQGG
FKEMIAKVSGDGAYGILKFESGGHRVQRVPETESQGRVHTSACTIAVMAEIPEADLPEIKSSDLKIDTFRSSGAGGQHVN
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMSVLAARIIQAEEARRAAVVSDTRRNLLGSGDRSDRIRTYNYPQSRVS
DHRINLTIYRLNEVMEGDLAALIEPVVLEYQADQLAALAEQN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=292, Percent_Identity=43.4931506849315, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI34577120, Length=338, Percent_Identity=36.3905325443787, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=42.0289855072464, Blast_Score=178, Evalue=6e-45,
Organism=Escherichia coli, GI1787462, Length=361, Percent_Identity=72.0221606648199, Blast_Score=529, Evalue=1e-151,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=38.0239520958084, Blast_Score=207, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=289, Percent_Identity=36.3321799307958, Blast_Score=178, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6321295, Length=336, Percent_Identity=39.2857142857143, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI19921226, Length=326, Percent_Identity=41.4110429447853, Blast_Score=224, Evalue=1e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_ALISL (B6EHH5)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262300.1
- GeneID:   6988640
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0790
- HOGENOM:   HBG629764
- OMA:   GEAGGYK
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40376; Mature: 40376

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASIRVKLETLVERYEEVQHLLGDPGVIGDQNKFRALSREYSQLEEVTQCFQAYEKVQE
CCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLVAAQEMAQEDDAEMREMAQDEIKEAKEASERLTDELQVLLIPKDPNDERNCFLEIRAG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECC
AGGDEAGIFAGNLFRMYSRFAEKKGWRIEVMSSHASEQGGFKEMIAKVSGDGAYGILKFE
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEEC
SGGHRVQRVPETESQGRVHTSACTIAVMAEIPEADLPEIKSSDLKIDTFRSSGAGGQHVN
CCCCCEEECCCCCCCCCEEHHHHEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCC
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMSVLAARIIQAEEARRAAVVSDTRRNLL
CCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSGDRSDRIRTYNYPQSRVSDHRINLTIYRLNEVMEGDLAALIEPVVLEYQADQLAALAE
CCCCCCCCCEECCCCHHHCCCCEEEEEEEEECHHHCCHHHHHHHHHHHHHCHHHHHHHHC
QN
CC
>Mature Secondary Structure
MKASIRVKLETLVERYEEVQHLLGDPGVIGDQNKFRALSREYSQLEEVTQCFQAYEKVQE
CCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLVAAQEMAQEDDAEMREMAQDEIKEAKEASERLTDELQVLLIPKDPNDERNCFLEIRAG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECC
AGGDEAGIFAGNLFRMYSRFAEKKGWRIEVMSSHASEQGGFKEMIAKVSGDGAYGILKFE
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEEC
SGGHRVQRVPETESQGRVHTSACTIAVMAEIPEADLPEIKSSDLKIDTFRSSGAGGQHVN
CCCCCEEECCCCCCCCCEEHHHHEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCC
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMSVLAARIIQAEEARRAAVVSDTRRNLL
CCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSGDRSDRIRTYNYPQSRVSDHRINLTIYRLNEVMEGDLAALIEPVVLEYQADQLAALAE
CCCCCCCCCEECCCCHHHCCCCEEEEEEEEECHHHCCHHHHHHHHHHHHHCHHHHHHHHC
QN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA