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The map label for this gene is hemK [H]

Identifier: 209694373

GI number: 209694373

Start: 877583

End: 878449

Strand: Direct

Name: hemK [H]

Synonym: VSAL_I0791

Alternate gene names: 209694373

Gene position: 877583-878449 (Clockwise)

Preceding gene: 209694372

Following gene: 209694374

Centisome position: 26.39

GC content: 39.79

Gene sequence:

>867_bases
ATGAGCATTTCTATTGAAGCGCTATTAATGCGTTCAATTCAATTGCTGAAACTTGAGGGGAGTGATTCTCCTCAAGTTGA
TGCTGCTGTATTGCTGTGTCATGTTTTAGATAAGCCAAGAAGCTACTTACTTACTTGGCCTGAAAAGCTCGTCGCTGAAA
CCGAATACAATGCATTTGAATCATTATTATCTCGACGCCTTTCTGGTGAACCAATCGCTTACATTATTGGTTATCGAGAG
TTTTGGTCATTACCGTTAAAAGTCTCGCCAACAACATTAATTCCTCGTCCTGATACTGAACGTTTGGTAGAAGTTGCGTT
AGAGCATCTATCGCCATCATCTGAAAAGGTATTGGATTTGGGGACGGGAACAGGAGCCATTGCATTAGCTATCGCCTCTG
AAATGCCTGATATGTGTGTTATTGGTGTTGATTATCAATATGACGCGGTTGCGTTAGCGAAAGATAATGCCAAAGACAAC
AATATATTGAATACTGAATTTCGTCAGGGAAGTTGGTTTGATCCGATTAGAATGGATGAACAGTTTTCAATTATTGTGAG
TAACCCGCCTTATATTGATGGGAATGATCCCCATTTGTCTCAAGGTGATGTACGGTTCGAGCCTCAAACCGCTTTGGTTG
CTGAGAAAGAAGGTTTTGCGGACTTAATCCATATTATGGAGCACAGTCGTACTCATTTAGTTGAGAACGGTTGGTTATTA
ATGGAACATGGCTTTGAACAAGGTAAGCAATTACGTGATTACTTTGAAGAATATGGCTTCATTAATGTTAAAACAGAACA
AGATTACGCTGGCAATGATCGAGTTACATTAGGTCAGTGGATCACAAACACAGAGAATAAAGGATAA

Upstream 100 bases:

>100_bases
GTTTGAATGAAGTGATGGAAGGGGATCTAGCGGCGTTGATTGAACCTGTCGTATTAGAATATCAAGCTGATCAACTAGCG
GCTTTAGCTGAACAAAACTA

Downstream 100 bases:

>100_bases
TTCATGTATACCGCTGTTAAACACATTCACTTATTTATGATCGCTTGTAGCGTACTATTATTTATCGTTCGTTATGCGTT
AATGATGGTTGATTCCGATC

Product: protein methyltransferase HemK

Products: NA

Alternate protein names: M.VchAHemK2P [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSISIEALLMRSIQLLKLEGSDSPQVDAAVLLCHVLDKPRSYLLTWPEKLVAETEYNAFESLLSRRLSGEPIAYIIGYRE
FWSLPLKVSPTTLIPRPDTERLVEVALEHLSPSSEKVLDLGTGTGAIALAIASEMPDMCVIGVDYQYDAVALAKDNAKDN
NILNTEFRQGSWFDPIRMDEQFSIIVSNPPYIDGNDPHLSQGDVRFEPQTALVAEKEGFADLIHIMEHSRTHLVENGWLL
MEHGFEQGKQLRDYFEEYGFINVKTEQDYAGNDRVTLGQWITNTENKG

Sequences:

>Translated_288_residues
MSISIEALLMRSIQLLKLEGSDSPQVDAAVLLCHVLDKPRSYLLTWPEKLVAETEYNAFESLLSRRLSGEPIAYIIGYRE
FWSLPLKVSPTTLIPRPDTERLVEVALEHLSPSSEKVLDLGTGTGAIALAIASEMPDMCVIGVDYQYDAVALAKDNAKDN
NILNTEFRQGSWFDPIRMDEQFSIIVSNPPYIDGNDPHLSQGDVRFEPQTALVAEKEGFADLIHIMEHSRTHLVENGWLL
MEHGFEQGKQLRDYFEEYGFINVKTEQDYAGNDRVTLGQWITNTENKG
>Mature_287_residues
SISIEALLMRSIQLLKLEGSDSPQVDAAVLLCHVLDKPRSYLLTWPEKLVAETEYNAFESLLSRRLSGEPIAYIIGYREF
WSLPLKVSPTTLIPRPDTERLVEVALEHLSPSSEKVLDLGTGTGAIALAIASEMPDMCVIGVDYQYDAVALAKDNAKDNN
ILNTEFRQGSWFDPIRMDEQFSIIVSNPPYIDGNDPHLSQGDVRFEPQTALVAEKEGFADLIHIMEHSRTHLVENGWLLM
EHGFEQGKQLRDYFEEYGFINVKTEQDYAGNDRVTLGQWITNTENKG

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=251, Percent_Identity=29.0836653386454, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI1787463, Length=264, Percent_Identity=53.4090909090909, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI87082085, Length=239, Percent_Identity=28.4518828451883, Blast_Score=86, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324266, Length=175, Percent_Identity=29.7142857142857, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582226, Length=238, Percent_Identity=25.6302521008403, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 32461; Mature: 32330

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISIEALLMRSIQLLKLEGSDSPQVDAAVLLCHVLDKPRSYLLTWPEKLVAETEYNAFE
CCCCHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHCCHHHHH
SLLSRRLSGEPIAYIIGYREFWSLPLKVSPTTLIPRPDTERLVEVALEHLSPSSEKVLDL
HHHHHHCCCCCEEEEEECHHHHCCCEEECCCEECCCCCHHHHHHHHHHHCCCCCHHEEEE
GTGTGAIALAIASEMPDMCVIGVDYQYDAVALAKDNAKDNNILNTEFRQGSWFDPIRMDE
CCCCCCEEEEEHHCCCCEEEEECCCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCC
QFSIIVSNPPYIDGNDPHLSQGDVRFEPQTALVAEKEGFADLIHIMEHSRTHLVENGWLL
CEEEEECCCCEECCCCCCCCCCCEEECCCCEEEECCCCHHHHHHHHHCCHHEEECCCEEE
MEHGFEQGKQLRDYFEEYGFINVKTEQDYAGNDRVTLGQWITNTENKG
EECCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEHHHCCCCCCCC
>Mature Secondary Structure 
SISIEALLMRSIQLLKLEGSDSPQVDAAVLLCHVLDKPRSYLLTWPEKLVAETEYNAFE
CCCHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHCCHHHHH
SLLSRRLSGEPIAYIIGYREFWSLPLKVSPTTLIPRPDTERLVEVALEHLSPSSEKVLDL
HHHHHHCCCCCEEEEEECHHHHCCCEEECCCEECCCCCHHHHHHHHHHHCCCCCHHEEEE
GTGTGAIALAIASEMPDMCVIGVDYQYDAVALAKDNAKDNNILNTEFRQGSWFDPIRMDE
CCCCCCEEEEEHHCCCCEEEEECCCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCC
QFSIIVSNPPYIDGNDPHLSQGDVRFEPQTALVAEKEGFADLIHIMEHSRTHLVENGWLL
CEEEEECCCCEECCCCCCCCCCCEEECCCCEEEECCCCHHHHHHHHHCCHHEEECCCEEE
MEHGFEQGKQLRDYFEEYGFINVKTEQDYAGNDRVTLGQWITNTENKG
EECCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]