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The map label for this gene is amiD [H]

Identifier: 209694359

GI number: 209694359

Start: 864223

End: 864978

Strand: Reverse

Name: amiD [H]

Synonym: VSAL_I0777

Alternate gene names: 209694359

Gene position: 864978-864223 (Counterclockwise)

Preceding gene: 209694360

Following gene: 209694358

Centisome position: 26.01

GC content: 41.01

Gene sequence:

>756_bases
ATGTGTCATATTGATTATAATTCTTATCGCTCTGTTTCCAGTTACAACCGCAGAGTTCGTTTTCTTGTTATGCATTATAC
GGCTATTAATTTCTCCGCATCCATTAACGCATTAACAGGAAAAGAGGCAACCGTAAGTGCTCATTACCTCGTCCCTAACC
CTTTAGATATCACTTATCAAAACGCAGGTTTTGGGGATATGAGGATCTTTAATTTAGTCGATGAAAATGAAAGGGCTTGG
CACGCAGGCGTCAGCTCATGGGCGGGACGTAATGGGTTAAATGATTCTTCTATTGGGATTGAAATAGTTAATGAAGCTTC
TGATGATAAAGGAGTGTTTACTTTTCCACCTTATAGCCCAACTCAAATTCAAGCACTAATATCCCTATCAAAAAATATAT
TGCAACGATACCCAGATCTCTCACCGACTAACGTCGTCGGTCACAGTGATATCGCCGTCGGAAGAAAGGTCGATCCAGGC
CCTGCTTTTCCATGGAAAGCACTTTACGATGAGGGGGTTGGGGCTTGGTATGACGAAGACAAAAAACAAAAATACACCAC
TAAACTCAGCACTGGCTTACCTGATAAAAAGGACATATTAGCTGCCTTTAAAAAATATGGTTATGCCGTTGACGGTGAAT
ATTCACTGCTTGTTCGTGCGTTTCAATTGCACTTTAGACCTCGAAGAACTACTGGGGTAGCGGATCTTGAAACCCTCTCC
ATACTGTTTGCACTCGTTGAAAAATATTTTAAATAG

Upstream 100 bases:

>100_bases
TTGTCAAATTTATGTTAGGAATTGCATTATATTGTATTGTGTTGTATTAAAAAAAAAGATGTTTATTGTAATTTCACTTA
CAACAACATTGGTAAATACG

Downstream 100 bases:

>100_bases
CGCAACATTGAACACAAAAGAACAAACATTAATCTAAGTTTTAAAATTAAGGATTTTACTATGGAACCTACATTAACCTC
ATCAATCAAATTTAATGCTG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MCHIDYNSYRSVSSYNRRVRFLVMHYTAINFSASINALTGKEATVSAHYLVPNPLDITYQNAGFGDMRIFNLVDENERAW
HAGVSSWAGRNGLNDSSIGIEIVNEASDDKGVFTFPPYSPTQIQALISLSKNILQRYPDLSPTNVVGHSDIAVGRKVDPG
PAFPWKALYDEGVGAWYDEDKKQKYTTKLSTGLPDKKDILAAFKKYGYAVDGEYSLLVRAFQLHFRPRRTTGVADLETLS
ILFALVEKYFK

Sequences:

>Translated_251_residues
MCHIDYNSYRSVSSYNRRVRFLVMHYTAINFSASINALTGKEATVSAHYLVPNPLDITYQNAGFGDMRIFNLVDENERAW
HAGVSSWAGRNGLNDSSIGIEIVNEASDDKGVFTFPPYSPTQIQALISLSKNILQRYPDLSPTNVVGHSDIAVGRKVDPG
PAFPWKALYDEGVGAWYDEDKKQKYTTKLSTGLPDKKDILAAFKKYGYAVDGEYSLLVRAFQLHFRPRRTTGVADLETLS
ILFALVEKYFK
>Mature_251_residues
MCHIDYNSYRSVSSYNRRVRFLVMHYTAINFSASINALTGKEATVSAHYLVPNPLDITYQNAGFGDMRIFNLVDENERAW
HAGVSSWAGRNGLNDSSIGIEIVNEASDDKGVFTFPPYSPTQIQALISLSKNILQRYPDLSPTNVVGHSDIAVGRKVDPG
PAFPWKALYDEGVGAWYDEDKKQKYTTKLSTGLPDKKDILAAFKKYGYAVDGEYSLLVRAFQLHFRPRRTTGVADLETLS
ILFALVEKYFK

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=250, Percent_Identity=44.4, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1786300, Length=121, Percent_Identity=40.495867768595, Blast_Score=81, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 28178; Mature: 28178

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCHIDYNSYRSVSSYNRRVRFLVMHYTAINFSASINALTGKEATVSAHYLVPNPLDITYQ
CEECCCCCHHCHHHHCCEEEEEEEEEEEEECCCEEEEECCCCEEEEEEEECCCCCEEEEC
NAGFGDMRIFNLVDENERAWHAGVSSWAGRNGLNDSSIGIEIVNEASDDKGVFTFPPYSP
CCCCCCEEEEEEECCCCCHHHHCHHHHCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCH
TQIQALISLSKNILQRYPDLSPTNVVGHSDIAVGRKVDPGPAFPWKALYDEGVGAWYDED
HHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC
KKQKYTTKLSTGLPDKKDILAAFKKYGYAVDGEYSLLVRAFQLHFRPRRTTGVADLETLS
HHHHHHHHHHCCCCCHHHHHHHHHHHCCEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
ILFALVEKYFK
HHHHHHHHHCC
>Mature Secondary Structure
MCHIDYNSYRSVSSYNRRVRFLVMHYTAINFSASINALTGKEATVSAHYLVPNPLDITYQ
CEECCCCCHHCHHHHCCEEEEEEEEEEEEECCCEEEEECCCCEEEEEEEECCCCCEEEEC
NAGFGDMRIFNLVDENERAWHAGVSSWAGRNGLNDSSIGIEIVNEASDDKGVFTFPPYSP
CCCCCCEEEEEEECCCCCHHHHCHHHHCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCH
TQIQALISLSKNILQRYPDLSPTNVVGHSDIAVGRKVDPGPAFPWKALYDEGVGAWYDED
HHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC
KKQKYTTKLSTGLPDKKDILAAFKKYGYAVDGEYSLLVRAFQLHFRPRRTTGVADLETLS
HHHHHHHHHHCCCCCHHHHHHHHHHHCCEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
ILFALVEKYFK
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]