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The map label for this gene is yafC [C]

Identifier: 209694335

GI number: 209694335

Start: 834157

End: 835029

Strand: Direct

Name: yafC [C]

Synonym: VSAL_I0747

Alternate gene names: 209694335

Gene position: 834157-835029 (Clockwise)

Preceding gene: 209694328

Following gene: 209694336

Centisome position: 25.09

GC content: 39.4

Gene sequence:

>873_bases
GTGATTAATTGGGAAGGGGTAACAGAATTTGTTGCTGTAGCAGAAGCGCAAAGTTTTACTGGGGCAGCAAAAGCACTGGA
TACTTCAGTAGCACAAATTAGTCGTAAAGTCGCTGCATTAGAAGAACGTTTATCGATAAAACTTTTAATACGAACGACTC
GAAAAGTATCAGTAACCGAAGCTGGACAAACTTATTACCAACATTGTCGTCATTTAGTTGAAGGCTTAAAACAAGCAGAT
AGAGCGATTACTGAATTAAATGCGACCCCTCAAGGGCGTTTAAAAATCACGTCATCAGTCACGTATGGTGAGCAGCTTAT
CGCGCCATTGCTTAGCGATTTCATGCTCATGTATCCACAACTTGAACTTGAACTTGTGTTATCAAATCAAACGTTCGATC
TCGTTGAGCAAGATTTTGATTTGGCGATTCGTTTAGGTCGATTAAGTGATTCAACAATGATGGCAAAACGCTTATCTAGC
CGACAAGTACGGGTATGCGCATCGCCTGCTTATATTGAAAAATATGGTGAACCACATACCTTGTCTGAACTTTCTCAGCA
TAATTGTTTAGTTGGCAGCTTGCCTATTTGGCGATTCATTGAAAATAAAAAAGAAAAAAGCGTAAGAGTAAAGGGATCGG
TTCATTGCAATAGTGGCGCAGCCCTAACGAATGCCGCTTTAAAAGGATTAGGCATTGTTCAGTTACCTGATTTTTATGTT
CAAGAATCAATGGATAAAGGGGAATTAGTCGAAATACTGGTTCCATATAGAGAGCCTAAAGAAGGTATTTGGGCGGTATA
TCCAAACAATCGACACCTTTCAACAAAAGTTCGTTTATTGGTTGAGTTCTTAGCAAAAGAGTTAGCAGAATAG

Upstream 100 bases:

>100_bases
CTTGTTGTGTGCCATTATAAATATTTCTCCTCATTTGATAATACGGTAGAATGGTAAATAATCTTTACATATACGTAATA
ATACTAATGAGGGACTGGTT

Downstream 100 bases:

>100_bases
AATGACAGATTTAGACGAGAATAAGAGCGCGACGTTCTATATGCAAAGAGCGATAGAGCTTGCCTCAATCGCAGAAGGTG
AGGGGGAAGTTCCTGTCGGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MINWEGVTEFVAVAEAQSFTGAAKALDTSVAQISRKVAALEERLSIKLLIRTTRKVSVTEAGQTYYQHCRHLVEGLKQAD
RAITELNATPQGRLKITSSVTYGEQLIAPLLSDFMLMYPQLELELVLSNQTFDLVEQDFDLAIRLGRLSDSTMMAKRLSS
RQVRVCASPAYIEKYGEPHTLSELSQHNCLVGSLPIWRFIENKKEKSVRVKGSVHCNSGAALTNAALKGLGIVQLPDFYV
QESMDKGELVEILVPYREPKEGIWAVYPNNRHLSTKVRLLVEFLAKELAE

Sequences:

>Translated_290_residues
MINWEGVTEFVAVAEAQSFTGAAKALDTSVAQISRKVAALEERLSIKLLIRTTRKVSVTEAGQTYYQHCRHLVEGLKQAD
RAITELNATPQGRLKITSSVTYGEQLIAPLLSDFMLMYPQLELELVLSNQTFDLVEQDFDLAIRLGRLSDSTMMAKRLSS
RQVRVCASPAYIEKYGEPHTLSELSQHNCLVGSLPIWRFIENKKEKSVRVKGSVHCNSGAALTNAALKGLGIVQLPDFYV
QESMDKGELVEILVPYREPKEGIWAVYPNNRHLSTKVRLLVEFLAKELAE
>Mature_290_residues
MINWEGVTEFVAVAEAQSFTGAAKALDTSVAQISRKVAALEERLSIKLLIRTTRKVSVTEAGQTYYQHCRHLVEGLKQAD
RAITELNATPQGRLKITSSVTYGEQLIAPLLSDFMLMYPQLELELVLSNQTFDLVEQDFDLAIRLGRLSDSTMMAKRLSS
RQVRVCASPAYIEKYGEPHTLSELSQHNCLVGSLPIWRFIENKKEKSVRVKGSVHCNSGAALTNAALKGLGIVQLPDFYV
QESMDKGELVEILVPYREPKEGIWAVYPNNRHLSTKVRLLVEFLAKELAE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=35.2112676056338, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1789440, Length=281, Percent_Identity=33.0960854092527, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI87081978, Length=286, Percent_Identity=33.9160839160839, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=32.0557491289199, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1789639, Length=286, Percent_Identity=34.2657342657343, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1787128, Length=290, Percent_Identity=30.6896551724138, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1787589, Length=282, Percent_Identity=29.0780141843972, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1790011, Length=295, Percent_Identity=24.7457627118644, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786448, Length=282, Percent_Identity=25.886524822695, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI157672245, Length=119, Percent_Identity=35.2941176470588, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789173, Length=243, Percent_Identity=25.1028806584362, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788706, Length=250, Percent_Identity=25.6, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787601, Length=171, Percent_Identity=24.5614035087719, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI145693105, Length=148, Percent_Identity=27.027027027027, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786713, Length=274, Percent_Identity=26.2773722627737, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788481, Length=283, Percent_Identity=22.6148409893993, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32452; Mature: 32452

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINWEGVTEFVAVAEAQSFTGAAKALDTSVAQISRKVAALEERLSIKLLIRTTRKVSVTE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCEEEEHH
AGQTYYQHCRHLVEGLKQADRAITELNATPQGRLKITSSVTYGEQLIAPLLSDFMLMYPQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCC
LELELVLSNQTFDLVEQDFDLAIRLGRLSDSTMMAKRLSSRQVRVCASPAYIEKYGEPHT
EEEEEEECCCCHHHHHCCHHHEEEECCCCCHHHHHHHHCCCCEEEECCHHHHHHCCCCCH
LSELSQHNCLVGSLPIWRFIENKKEKSVRVKGSVHCNSGAALTNAALKGLGIVQLPDFYV
HHHHHHCCCEEECCHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHCCCEEECCCHHH
QESMDKGELVEILVPYREPKEGIWAVYPNNRHLSTKVRLLVEFLAKELAE
HHCCCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MINWEGVTEFVAVAEAQSFTGAAKALDTSVAQISRKVAALEERLSIKLLIRTTRKVSVTE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCEEEEHH
AGQTYYQHCRHLVEGLKQADRAITELNATPQGRLKITSSVTYGEQLIAPLLSDFMLMYPQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCC
LELELVLSNQTFDLVEQDFDLAIRLGRLSDSTMMAKRLSSRQVRVCASPAYIEKYGEPHT
EEEEEEECCCCHHHHHCCHHHEEEECCCCCHHHHHHHHCCCCEEEECCHHHHHHCCCCCH
LSELSQHNCLVGSLPIWRFIENKKEKSVRVKGSVHCNSGAALTNAALKGLGIVQLPDFYV
HHHHHHCCCEEECCHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHCCCEEECCCHHH
QESMDKGELVEILVPYREPKEGIWAVYPNNRHLSTKVRLLVEFLAKELAE
HHCCCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]