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The map label for this gene is trmD

Identifier: 209694242

GI number: 209694242

Start: 723697

End: 724446

Strand: Direct

Name: trmD

Synonym: VSAL_I0651

Alternate gene names: 209694242

Gene position: 723697-724446 (Clockwise)

Preceding gene: 209694241

Following gene: 209694243

Centisome position: 21.76

GC content: 44.53

Gene sequence:

>750_bases
ATGTGGGTTGGGGTTATAAGCCTATTTCCTGAAATGTTCCGTTCGGTTACCGATTTTGGGGTAACTAGCCAAGCAATTAA
AAAAGGTCTTTTATCTATAGAGACATGGAATCCTAGAGATTTCACTCAAGATAAACATCGCACTGTTGATGACCGACCTT
ATGGTGGTGGTCCTGGTATGTTAATGATGGTACAGCCTTTGCGCGATGCCATTACTGCAGCCAGAGAAGCCGCTCCAGGC
AAAACGAAAGTGATTTACCTTTCACCTCAAGGCAGAACGCTGAATCAAGCTGGTGTTGAGGAGTTGGCAACAAACGAGAA
CTTAGTTCTTATCTGTGGCCGATACGAAGGCGTAGATGAGCGCATAATACAATCTGAAGTTGACGAAGAATGGTCAATTG
GCGATTTTGTGCTTACAGGCGGGGAACTCCCTGCTATGACGTTGATTGATTCTGTCTCTCGGTTTGTTCCGGGTGTACTT
GGAGATTTTGCGTCCGCTGAAGAAGATTCTTTTGCAGACGGTTTGTTAGATTGTCCGCACTATACAAGGCCTGAGGTCTT
GGATGGTAAGGAAGTGCCTAGTGTACTTAAATCTGGAAATCATAAAGATATCGCTCGTTGGCGAATGAAACAATCGTTAG
GCCGTACTTGGCTAAGAAGACCAGAGCTCCTGGGAAATCTAGCTCTGACTGACGAACAGGAATTATTACTGGCTGAATTT
GTTCGTGAAGAACATCAGCAAAGTAAGTAA

Upstream 100 bases:

>100_bases
TTTATTGATGAGCAAGTGATCAAAGTAATTGATCGTGAGGCTCAACGGATCGAAGTTGACTGGGATCCTGGGTTCTAAAC
CCTAGGTAAAGCGAGGGCAC

Downstream 100 bases:

>100_bases
TTAAGAAATTTAGTATCAGTTTATTCTAGGATATACAAAAATGAGCAACATCATTAAAGCAATTGAAGATGCGCAACTAA
AGCAAGATCTACCTAAGTTC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MWVGVISLFPEMFRSVTDFGVTSQAIKKGLLSIETWNPRDFTQDKHRTVDDRPYGGGPGMLMMVQPLRDAITAAREAAPG
KTKVIYLSPQGRTLNQAGVEELATNENLVLICGRYEGVDERIIQSEVDEEWSIGDFVLTGGELPAMTLIDSVSRFVPGVL
GDFASAEEDSFADGLLDCPHYTRPEVLDGKEVPSVLKSGNHKDIARWRMKQSLGRTWLRRPELLGNLALTDEQELLLAEF
VREEHQQSK

Sequences:

>Translated_249_residues
MWVGVISLFPEMFRSVTDFGVTSQAIKKGLLSIETWNPRDFTQDKHRTVDDRPYGGGPGMLMMVQPLRDAITAAREAAPG
KTKVIYLSPQGRTLNQAGVEELATNENLVLICGRYEGVDERIIQSEVDEEWSIGDFVLTGGELPAMTLIDSVSRFVPGVL
GDFASAEEDSFADGLLDCPHYTRPEVLDGKEVPSVLKSGNHKDIARWRMKQSLGRTWLRRPELLGNLALTDEQELLLAEF
VREEHQQSK
>Mature_249_residues
MWVGVISLFPEMFRSVTDFGVTSQAIKKGLLSIETWNPRDFTQDKHRTVDDRPYGGGPGMLMMVQPLRDAITAAREAAPG
KTKVIYLSPQGRTLNQAGVEELATNENLVLICGRYEGVDERIIQSEVDEEWSIGDFVLTGGELPAMTLIDSVSRFVPGVL
GDFASAEEDSFADGLLDCPHYTRPEVLDGKEVPSVLKSGNHKDIARWRMKQSLGRTWLRRPELLGNLALTDEQELLLAEF
VREEHQQSK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=247, Percent_Identity=77.7327935222672, Blast_Score=397, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_ALISL (B6EGC1)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262170.1
- GeneID:   6989106
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0651
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27789; Mature: 27789

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWVGVISLFPEMFRSVTDFGVTSQAIKKGLLSIETWNPRDFTQDKHRTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCHHHCCCCCCCCCCCCCC
LMMVQPLRDAITAAREAAPGKTKVIYLSPQGRTLNQAGVEELATNENLVLICGRYEGVDE
EEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHH
RIIQSEVDEEWSIGDFVLTGGELPAMTLIDSVSRFVPGVLGDFASAEEDSFADGLLDCPH
HHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
YTRPEVLDGKEVPSVLKSGNHKDIARWRMKQSLGRTWLRRPELLGNLALTDEQELLLAEF
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHH
VREEHQQSK
HHHHHHCCC
>Mature Secondary Structure
MWVGVISLFPEMFRSVTDFGVTSQAIKKGLLSIETWNPRDFTQDKHRTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCHHHCCCCCCCCCCCCCC
LMMVQPLRDAITAAREAAPGKTKVIYLSPQGRTLNQAGVEELATNENLVLICGRYEGVDE
EEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHH
RIIQSEVDEEWSIGDFVLTGGELPAMTLIDSVSRFVPGVLGDFASAEEDSFADGLLDCPH
HHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
YTRPEVLDGKEVPSVLKSGNHKDIARWRMKQSLGRTWLRRPELLGNLALTDEQELLLAEF
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHH
VREEHQQSK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA