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The map label for this gene is pnp [H]

Identifier: 209694194

GI number: 209694194

Start: 664832

End: 666988

Strand: Direct

Name: pnp [H]

Synonym: VSAL_I0602

Alternate gene names: 209694194

Gene position: 664832-666988 (Clockwise)

Preceding gene: 209694193

Following gene: 209694195

Centisome position: 19.99

GC content: 44.23

Gene sequence:

>2157_bases
ATGTGTATTGAGGCCAAAGGAAAAAAAATGTTCGAGAAACCAGTCGTAAAATCATTCCAGTACGGTAACCACACCGTAAC
ACTAGAGACGGGCGTAATGGCACGTCAAGCAACTGCAGCAGTAATGGCAAGCATGGACGATACATCAGTATTCGTTTCTG
TTGTTGCTAAGAAAGAAGCAGTAGCGGGTCAAGATTTCTTCCCACTAACCGTTAACTACCAAGAGCGTACTTACGCTGCT
GGTAAAATCCCAGGTGGTTTCTTTAAGCGTGAAGGCCGTCCTTCTGAAGGCGAAACACTAACGGCTCGTCTAATTGACCG
TCCAATTCGTCCACTTTTCCCAAGTGCGTTTAAAAACGAAGTTCAAGTTATCGCTACGGTTGTTTCTATCAACCCTGACG
TAAACCCAGACATGATCACTATGATCGCAACGTCTGCGGCACTTTCTATTGCTGGTGTTCCATTTAATGGTCCTATCGGT
GCTGCACGTGTTGGTCATATCAACGGTGAGCTTGTTCTTAACCCATCAAACACTGAGCTTGCAAACTCTAAACTAGACCT
AGTTGTGTCTGGTACAGAAGCTGCTGTACTAATGGTTGAATCTGAAGCAGATAACCTATCTGAAGAAGAAATGCTTTCTG
CTGTTGTATTTGGTCATGACCAACAACAAGTAGTAATCAAAGCAATCAACGAGTTTGCTGCTGAAGTTGCAACGCCGTCT
TGGAACTGGGAAGCACCAGTAGTAAACGCAGAGCTTAAAGCACAAGTTGCTGAACTTGCAGAAACTCGTCTTTCTGAAGC
GTACCAAATTACTGAAAAAATGGCGCGTTACGAGCAAGTTGGCGCAATCAAAAGTGAAGTTGTTGCAGCACTTCTAGTTC
AAAATGAAGCACTAGACGAACGTGAAATCCGCGGTATGCTTGGCGCTCTAGAGAAAAACGTAGTACGTAGCCGTATTATT
GCTGGTCACCCACGTATCGATGGCCGTGAAAAAGACATGGTTCGTGCGCTAGACGTACGTACTGGTGTTCTTCCTCGTAC
TCACGGTTCAGCTCTATTTACTCGTGGTGAAACTCAAGCACTTGTTACTGCAACGCTTGGTACACAACGTGATGCTCAAA
TCATCGATAGCTTAATGGGTGAGAAGAAAGATCACTTCCTTCTACACTACAACTTTCCTCCATACTGTGTTGGTGAAACT
GGCTTTGTTGGTTCACCTAAGCGTCGTGAAATCGGCCACGGTAAACTGGCTAAACGCGGTATCGCAGCAGTTATGCCTTC
TGTTGAAGAATTCCCATACACAGTACGTGTCGTATCGGAAATCACTGAATCTAACGGTTCATCTTCAATGGCTTCTGTAT
GTGGTACATCTCTAGCGCTTATGGATGCTGGTGTTCCAATTAAAGCGTCTGTTGCTGGTATCGCTATGGGTCTTGTTAAA
GAAGGCGACGATTTCGTTGTTCTTTCTGACATCCTTGGCGACGAAGATCACCTAGGTGACATGGACTTTAAAGTTGCAGG
TACTAACGCTGGTATTACTGCACTTCAAATGGACATCAAAATTGAAGGTATCACTAAAGAGATCATGCAGATCGCTCTTA
ACCAGGCACAAGGTGCGCGTAAGCATATCCTTACAGTAATGGATGAAGCAATCTCAGGCGCTCGTGAAGATATCTCACAA
TACGCTCCACGTATCCACACAATGAAAATCAGCTCTGATAAGATCAAAGATGTTATCGGTAAAGGTGGCGCGGTTATCCG
TGCTCTTTGTGAAGAAACGGGTACAACTATCGAAATCGAAGACGATGGCACAATCAAAATCGCTGCGACTGAAGGTGCTG
CTGCGAAAGAAGCTATCCGTCGTATCGAAGAAATTACTGCTGAAGTTGAAGTGGGTAAAATCTACCCAGGTAAAGTTATG
CGCATCGTTGATTTCGGTGCATTCGTAACGGTTCTTGGTCCTAAAGAAGGTCTAGTTCATATTTCTCAAATTGCTGAAGA
GCGTATTGAGAAAGTGGCAGATCATCTACAAGTTGGTCAAGAAGTTCAAACTAAAGTACTAGAAATAGACCGTCAAGGCC
GTATTCGTCTAAGTATTAAAGAAGCAACTGCAGAGCTAAACCCTGTTGCTGCAACTGAAGTAAAAGACGCAGAATAA

Upstream 100 bases:

>100_bases
ACTATTACAAGTTATCTTTTATTAAGTAAATTTGTACTAGTCGCGACTGGTAATATGTTGAACCATCTACGTAAAGTGTT
TTAGTCGCAGATAGAAATAT

Downstream 100 bases:

>100_bases
TCTGATTGATTACAGTCTTTTGATGTAAAGCGTGTTATAAAGGGAGCAGAGATGCTCCCTTTTTTTTGCATCAGGGCGAC
TAAAAATAGATTTATTGTAG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MCIEAKGKKMFEKPVVKSFQYGNHTVTLETGVMARQATAAVMASMDDTSVFVSVVAKKEAVAGQDFFPLTVNYQERTYAA
GKIPGGFFKREGRPSEGETLTARLIDRPIRPLFPSAFKNEVQVIATVVSINPDVNPDMITMIATSAALSIAGVPFNGPIG
AARVGHINGELVLNPSNTELANSKLDLVVSGTEAAVLMVESEADNLSEEEMLSAVVFGHDQQQVVIKAINEFAAEVATPS
WNWEAPVVNAELKAQVAELAETRLSEAYQITEKMARYEQVGAIKSEVVAALLVQNEALDEREIRGMLGALEKNVVRSRII
AGHPRIDGREKDMVRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQRDAQIIDSLMGEKKDHFLLHYNFPPYCVGET
GFVGSPKRREIGHGKLAKRGIAAVMPSVEEFPYTVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKASVAGIAMGLVK
EGDDFVVLSDILGDEDHLGDMDFKVAGTNAGITALQMDIKIEGITKEIMQIALNQAQGARKHILTVMDEAISGAREDISQ
YAPRIHTMKISSDKIKDVIGKGGAVIRALCEETGTTIEIEDDGTIKIAATEGAAAKEAIRRIEEITAEVEVGKIYPGKVM
RIVDFGAFVTVLGPKEGLVHISQIAEERIEKVADHLQVGQEVQTKVLEIDRQGRIRLSIKEATAELNPVAATEVKDAE

Sequences:

>Translated_718_residues
MCIEAKGKKMFEKPVVKSFQYGNHTVTLETGVMARQATAAVMASMDDTSVFVSVVAKKEAVAGQDFFPLTVNYQERTYAA
GKIPGGFFKREGRPSEGETLTARLIDRPIRPLFPSAFKNEVQVIATVVSINPDVNPDMITMIATSAALSIAGVPFNGPIG
AARVGHINGELVLNPSNTELANSKLDLVVSGTEAAVLMVESEADNLSEEEMLSAVVFGHDQQQVVIKAINEFAAEVATPS
WNWEAPVVNAELKAQVAELAETRLSEAYQITEKMARYEQVGAIKSEVVAALLVQNEALDEREIRGMLGALEKNVVRSRII
AGHPRIDGREKDMVRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQRDAQIIDSLMGEKKDHFLLHYNFPPYCVGET
GFVGSPKRREIGHGKLAKRGIAAVMPSVEEFPYTVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKASVAGIAMGLVK
EGDDFVVLSDILGDEDHLGDMDFKVAGTNAGITALQMDIKIEGITKEIMQIALNQAQGARKHILTVMDEAISGAREDISQ
YAPRIHTMKISSDKIKDVIGKGGAVIRALCEETGTTIEIEDDGTIKIAATEGAAAKEAIRRIEEITAEVEVGKIYPGKVM
RIVDFGAFVTVLGPKEGLVHISQIAEERIEKVADHLQVGQEVQTKVLEIDRQGRIRLSIKEATAELNPVAATEVKDAE
>Mature_718_residues
MCIEAKGKKMFEKPVVKSFQYGNHTVTLETGVMARQATAAVMASMDDTSVFVSVVAKKEAVAGQDFFPLTVNYQERTYAA
GKIPGGFFKREGRPSEGETLTARLIDRPIRPLFPSAFKNEVQVIATVVSINPDVNPDMITMIATSAALSIAGVPFNGPIG
AARVGHINGELVLNPSNTELANSKLDLVVSGTEAAVLMVESEADNLSEEEMLSAVVFGHDQQQVVIKAINEFAAEVATPS
WNWEAPVVNAELKAQVAELAETRLSEAYQITEKMARYEQVGAIKSEVVAALLVQNEALDEREIRGMLGALEKNVVRSRII
AGHPRIDGREKDMVRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQRDAQIIDSLMGEKKDHFLLHYNFPPYCVGET
GFVGSPKRREIGHGKLAKRGIAAVMPSVEEFPYTVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKASVAGIAMGLVK
EGDDFVVLSDILGDEDHLGDMDFKVAGTNAGITALQMDIKIEGITKEIMQIALNQAQGARKHILTVMDEAISGAREDISQ
YAPRIHTMKISSDKIKDVIGKGGAVIRALCEETGTTIEIEDDGTIKIAATEGAAAKEAIRRIEEITAEVEVGKIYPGKVM
RIVDFGAFVTVLGPKEGLVHISQIAEERIEKVADHLQVGQEVQTKVLEIDRQGRIRLSIKEATAELNPVAATEVKDAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=706, Percent_Identity=38.1019830028329, Blast_Score=440, Evalue=1e-123,
Organism=Escherichia coli, GI145693187, Length=693, Percent_Identity=76.7676767676768, Blast_Score=1106, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=730, Percent_Identity=31.7808219178082, Blast_Score=326, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI17535281, Length=86, Percent_Identity=50, Blast_Score=74, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320850, Length=101, Percent_Identity=34.6534653465347, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI281362905, Length=715, Percent_Identity=38.3216783216783, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651641, Length=715, Percent_Identity=38.3216783216783, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651643, Length=715, Percent_Identity=38.3216783216783, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI161079377, Length=655, Percent_Identity=38.4732824427481, Blast_Score=422, Evalue=1e-118,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77367; Mature: 77367

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCIEAKGKKMFEKPVVKSFQYGNHTVTLETGVMARQATAAVMASMDDTSVFVSVVAKKEA
CCCCCCCHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VAGQDFFPLTVNYQERTYAAGKIPGGFFKREGRPSEGETLTARLIDRPIRPLFPSAFKNE
HCCCCCEEEEEECCCCEEECCCCCCCHHCCCCCCCCCCEEEHHHHHCCCCCCCCHHHHHH
VQVIATVVSINPDVNPDMITMIATSAALSIAGVPFNGPIGAARVGHINGELVLNPSNTEL
HEEEEEEEECCCCCCCCHHHHHHHHHHHEEECCCCCCCCCCCEEEECCCEEEECCCCCCC
ANSKLDLVVSGTEAAVLMVESEADNLSEEEMLSAVVFGHDQQQVVIKAINEFAAEVATPS
CCCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
WNWEAPVVNAELKAQVAELAETRLSEAYQITEKMARYEQVGAIKSEVVAALLVQNEALDE
CCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
REIRGMLGALEKNVVRSRIIAGHPRIDGREKDMVRALDVRTGVLPRTHGSALFTRGETQA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCE
LVTATLGTQRDAQIIDSLMGEKKDHFLLHYNFPPYCVGETGFVGSPKRREIGHGKLAKRG
EEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
IAAVMPSVEEFPYTVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKASVAGIAMGLVK
HHHHCCCHHHCCHHHHHHHHHHHCCCCCHHHHHHCCHHEEHCCCCCCCHHHHHHHHHHHC
EGDDFVVLSDILGDEDHLGDMDFKVAGTNAGITALQMDIKIEGITKEIMQIALNQAQGAR
CCCCEEEEHHHCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHCCHH
KHILTVMDEAISGAREDISQYAPRIHTMKISSDKIKDVIGKGGAVIRALCEETGTTIEIE
HHHHHHHHHHHCCHHHHHHHHCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEEC
DDGTIKIAATEGAAAKEAIRRIEEITAEVEVGKIYPGKVMRIVDFGAFVTVLGPKEGLVH
CCCEEEEEECCCHHHHHHHHHHHHHHHHEEECEECCCCEEEEEECCCEEEEECCCCCHHH
ISQIAEERIEKVADHLQVGQEVQTKVLEIDRQGRIRLSIKEATAELNPVAATEVKDAE
HHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEEHHCCCCCCCEECCCCCCC
>Mature Secondary Structure
MCIEAKGKKMFEKPVVKSFQYGNHTVTLETGVMARQATAAVMASMDDTSVFVSVVAKKEA
CCCCCCCHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VAGQDFFPLTVNYQERTYAAGKIPGGFFKREGRPSEGETLTARLIDRPIRPLFPSAFKNE
HCCCCCEEEEEECCCCEEECCCCCCCHHCCCCCCCCCCEEEHHHHHCCCCCCCCHHHHHH
VQVIATVVSINPDVNPDMITMIATSAALSIAGVPFNGPIGAARVGHINGELVLNPSNTEL
HEEEEEEEECCCCCCCCHHHHHHHHHHHEEECCCCCCCCCCCEEEECCCEEEECCCCCCC
ANSKLDLVVSGTEAAVLMVESEADNLSEEEMLSAVVFGHDQQQVVIKAINEFAAEVATPS
CCCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
WNWEAPVVNAELKAQVAELAETRLSEAYQITEKMARYEQVGAIKSEVVAALLVQNEALDE
CCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
REIRGMLGALEKNVVRSRIIAGHPRIDGREKDMVRALDVRTGVLPRTHGSALFTRGETQA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCE
LVTATLGTQRDAQIIDSLMGEKKDHFLLHYNFPPYCVGETGFVGSPKRREIGHGKLAKRG
EEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
IAAVMPSVEEFPYTVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKASVAGIAMGLVK
HHHHCCCHHHCCHHHHHHHHHHHCCCCCHHHHHHCCHHEEHCCCCCCCHHHHHHHHHHHC
EGDDFVVLSDILGDEDHLGDMDFKVAGTNAGITALQMDIKIEGITKEIMQIALNQAQGAR
CCCCEEEEHHHCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHCCHH
KHILTVMDEAISGAREDISQYAPRIHTMKISSDKIKDVIGKGGAVIRALCEETGTTIEIE
HHHHHHHHHHHCCHHHHHHHHCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEEC
DDGTIKIAATEGAAAKEAIRRIEEITAEVEVGKIYPGKVMRIVDFGAFVTVLGPKEGLVH
CCCEEEEEECCCHHHHHHHHHHHHHHHHEEECEECCCCEEEEEECCCEEEEECCCCCHHH
ISQIAEERIEKVADHLQVGQEVQTKVLEIDRQGRIRLSIKEATAELNPVAATEVKDAE
HHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEEHHCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA