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The map label for this gene is braB [H]

Identifier: 209694153

GI number: 209694153

Start: 614058

End: 615371

Strand: Direct

Name: braB [H]

Synonym: VSAL_I0560

Alternate gene names: 209694153

Gene position: 614058-615371 (Clockwise)

Preceding gene: 209694150

Following gene: 209694155

Centisome position: 18.47

GC content: 40.33

Gene sequence:

>1314_bases
GTGAAACAATCACTAAAACTAACCGATATCATAGCCGTTGGCTTTATGCTTTTTGCCTTCTTTTTAGGTGCAGGCAACAT
TATATTTCCACCAATTGCAGGTCAGATGGCCGGTGAACATGTTTACAGTGCAATGGGGGGGTTCCTTATTACTGCGGTAG
GTTTACCTCTTATGACCATTATTACTATTGGTGTAGCCGGTGGATCTTGGCAGCAACTTACTCGTGATTTACCACCAAAA
GTAGCGATTATTATGGCTATTTTGATGTTTGTTATCATTGGGCCTGCATTTGCTGCCCCTCGTACAGGTTTAGTTGCTTA
TGAAATGGCTGCAAAACCATTTATGTCTGCTGATGCCGGACAAATATCATTAACACTATTCTCAATCGCATTCTTCTCAA
TCGCAATGTTTTTTGCCTGGTCGCAAGGTAAGCTTATCGATACGATTGGTAAGTTTTTAACGCCGGCATTATTCATTGGT
TTAATCATTTTAGCGATTGCGGTATTTGTTAATCCACAAGGTGAGATTGTTGCAGCAACAGGGAACTATATCAATCAACC
ATTAACGACTGGTTTCTTTGAAGGTTATAACACCATGGATACGTTTGGTGCGTTAATGTTTGGTATTTTAATGATTGATG
CATTAAAAAAGAAAGGCATTACAGATCATAAAGCGACCACAAAATATTTAACCGCAGCAGGTTGTATTGCGGCATTAGGC
CTAGCATTTGTTTATGTCTCTTTATTCTACCTTGGTGCGACGTCTAGCTCTGTTGCTCCAGGTGCAACAAATGGCGGTGA
GATTTTAACTGCATATACTCATGCGCTATTTGGATCTTCTGGCCAAATCGTATTGTCTATTATAGTTATGATCGCTTGTT
TAACGACGGCTATTGGTCTTATTTCCGCTTGTGCTGATTACTTTAGTTCAATTTGTAAGGTGACTTATAAAACATGGGTT
ATTGTTGTTGGTGTCGCTTGCGCTATTGTTGCTAATGTTGGTCTAACTCAGCTTATAGCATTATCGGTACCAGTATTATT
CCTACTTTACCCTATTGCGATGGCTCTGATTGTTTTAGCATTTTTACGTCATATGATGCCGAATCCTCGTCTGGCATACC
GTGTCGTTGTTACTGTTGCAACTTGCTTTGCTGTATTAGATGCGGCGAAAGTGGCGGGTGCAGATATGTCGGCATTCTCG
ATGCTACCAATGTTTGATCGCGGTATGGCTTGGGTTATCCCTACGTTCGCTTCAATTATCATTGTTCGTTTTATTGGTAA
GAAAGAAGACGAGTTAGTGACTAAAGAAGTGTAA

Upstream 100 bases:

>100_bases
ATTGCGATTTAATGATTAATTGGTCATTATTCTTAAATAAATATCAAAAAAGATATAAAAATACGAAATCAAACACACAA
CATTATAAGAAGGGTTTTCT

Downstream 100 bases:

>100_bases
TTATCTCTAGATAATAGAGTTTCTTGCTTTTCTTCTATTAAGAGCTAATGCTGAACTTGTTAGATATTTAATTTTCTAAC
TGGGTCACATTAGCTCTTAT

Product: branched chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MKQSLKLTDIIAVGFMLFAFFLGAGNIIFPPIAGQMAGEHVYSAMGGFLITAVGLPLMTIITIGVAGGSWQQLTRDLPPK
VAIIMAILMFVIIGPAFAAPRTGLVAYEMAAKPFMSADAGQISLTLFSIAFFSIAMFFAWSQGKLIDTIGKFLTPALFIG
LIILAIAVFVNPQGEIVAATGNYINQPLTTGFFEGYNTMDTFGALMFGILMIDALKKKGITDHKATTKYLTAAGCIAALG
LAFVYVSLFYLGATSSSVAPGATNGGEILTAYTHALFGSSGQIVLSIIVMIACLTTAIGLISACADYFSSICKVTYKTWV
IVVGVACAIVANVGLTQLIALSVPVLFLLYPIAMALIVLAFLRHMMPNPRLAYRVVVTVATCFAVLDAAKVAGADMSAFS
MLPMFDRGMAWVIPTFASIIIVRFIGKKEDELVTKEV

Sequences:

>Translated_437_residues
MKQSLKLTDIIAVGFMLFAFFLGAGNIIFPPIAGQMAGEHVYSAMGGFLITAVGLPLMTIITIGVAGGSWQQLTRDLPPK
VAIIMAILMFVIIGPAFAAPRTGLVAYEMAAKPFMSADAGQISLTLFSIAFFSIAMFFAWSQGKLIDTIGKFLTPALFIG
LIILAIAVFVNPQGEIVAATGNYINQPLTTGFFEGYNTMDTFGALMFGILMIDALKKKGITDHKATTKYLTAAGCIAALG
LAFVYVSLFYLGATSSSVAPGATNGGEILTAYTHALFGSSGQIVLSIIVMIACLTTAIGLISACADYFSSICKVTYKTWV
IVVGVACAIVANVGLTQLIALSVPVLFLLYPIAMALIVLAFLRHMMPNPRLAYRVVVTVATCFAVLDAAKVAGADMSAFS
MLPMFDRGMAWVIPTFASIIIVRFIGKKEDELVTKEV
>Mature_437_residues
MKQSLKLTDIIAVGFMLFAFFLGAGNIIFPPIAGQMAGEHVYSAMGGFLITAVGLPLMTIITIGVAGGSWQQLTRDLPPK
VAIIMAILMFVIIGPAFAAPRTGLVAYEMAAKPFMSADAGQISLTLFSIAFFSIAMFFAWSQGKLIDTIGKFLTPALFIG
LIILAIAVFVNPQGEIVAATGNYINQPLTTGFFEGYNTMDTFGALMFGILMIDALKKKGITDHKATTKYLTAAGCIAALG
LAFVYVSLFYLGATSSSVAPGATNGGEILTAYTHALFGSSGQIVLSIIVMIACLTTAIGLISACADYFSSICKVTYKTWV
IVVGVACAIVANVGLTQLIALSVPVLFLLYPIAMALIVLAFLRHMMPNPRLAYRVVVTVATCFAVLDAAKVAGADMSAFS
MLPMFDRGMAWVIPTFASIIIVRFIGKKEDELVTKEV

Specific function: Component of the LIV-II transport system for branched- chain amino acids. BraB is specific for isoleucine, leucine and valine. The LIV-II transport system is coupled to sodium and lithium ions [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=439, Percent_Identity=39.1799544419134, Blast_Score=325, Evalue=4e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46345; Mature: 46345

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSLKLTDIIAVGFMLFAFFLGAGNIIFPPIAGQMAGEHVYSAMGGFLITAVGLPLMTI
CCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITIGVAGGSWQQLTRDLPPKVAIIMAILMFVIIGPAFAAPRTGLVAYEMAAKPFMSADAG
HHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHCCCCCCCCC
QISLTLFSIAFFSIAMFFAWSQGKLIDTIGKFLTPALFIGLIILAIAVFVNPQGEIVAAT
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
GNYINQPLTTGFFEGYNTMDTFGALMFGILMIDALKKKGITDHKATTKYLTAAGCIAALG
CCHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LAFVYVSLFYLGATSSSVAPGATNGGEILTAYTHALFGSSGQIVLSIIVMIACLTTAIGL
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ISACADYFSSICKVTYKTWVIVVGVACAIVANVGLTQLIALSVPVLFLLYPIAMALIVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRHMMPNPRLAYRVVVTVATCFAVLDAAKVAGADMSAFSMLPMFDRGMAWVIPTFASII
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHCCHHHHHHHHHHHH
IVRFIGKKEDELVTKEV
HHHHHCCCCHHHHCCCC
>Mature Secondary Structure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HHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2111004; 10984043 [H]