Click here to switch to the map view.

The map label for this gene is pgk [H]

Identifier: 209694149

GI number: 209694149

Start: 609091

End: 610254

Strand: Direct

Name: pgk [H]

Synonym: VSAL_I0556

Alternate gene names: 209694149

Gene position: 609091-610254 (Clockwise)

Preceding gene: 209694148

Following gene: 209694150

Centisome position: 18.32

GC content: 42.87

Gene sequence:

>1164_bases
ATGTCTGTAATCAAGATGACTGACCTGGAACTTGCAGGTAAACGCGTATTTATCCGTGCTGACCTAAACGTACCAGTAAA
AGATGGCAAAGTAACTTCTGATGCACGTATTCTTGCATCTCTACCAACGATTAAGCTTTGCTTAGAAGCTGGCGCAAAAG
TAATGGTTACTTCTCACCTTGGTCGTCCAACGGAAGGCGAATACAACGAAGAGTTCTCTCTAGCTCCTGTAGTTAACTAC
TTAAATGACGCACTAGATTGTGACGTTAAACTAGCTAAAGATTACCTAGATGGTCTTGAATTAAATGCTGGTGAGCTAGT
TGTTCTTGAAAACGTTCGCTTTAACAAAGGCGAGAAGAAGAACGAAGAAGCGCTTTCTAAGAAATACGCAGCACTATGTG
ACATATTCGTAATGGATGCATTTGGTACCGCTCACCGTGCTCAAGCGTCAACTCACGGTGTTGGCATGAACGCTCCTATC
GCGTGTGCTGGTCCTCTTCTAGCTGCTGAACTTGAAGCTCTAGGCAAAGCAATGGACAATCCTGCTCGTCCACTTGTTGC
TATCGTTGGTGGTTCTAAAGTATCAACTAAGCTAACTGTTCTTGAGTCTCTATCTAAAATTGCTGACCAACTTGTTGTTG
GTGGTGGTATCGCGAATACATTCATCGCAGCTCAAGGTCACAATGTTGGTAAATCTCTATACGAAGCAGATTTAGTAGAA
ACAGCTAAAAAATTAATGGTTGAATGTGCAATTCCAGTAGCTACTGATGTTGCATGTGCAAAAGCATTTGATGAAAACGC
TGAAGCTGAAATCAAAAACGTTGCAGATGTTCAAGACGACGACATGATCTTCGACCTTGGTCCTGATTCAACAGCCGTTC
TAGCTGACATCATTAAGAATGCAAAAACAATTCTTTGGAATGGCCCAGTTGGCGTATTTGAATTCAAAAACTTTGAAGCA
GGTACTGCGGGTATTTCTAAAGCAATCGCTGATTCTGAAGGTTTCTCTGTTGCGGGTGGCGGTGATACGCTAGCGGCTAT
CGATAAGTTCGGAATCAAAGCTGACGTTTCTTACATTTCTACAGGTGGCGGTGCATTCCTTGAGTTCGTTGAAGGTAAAG
TACTTCCTGCAGTAGCAATGCTTGAAGAGCGTGCTAAAGCGTAA

Upstream 100 bases:

>100_bases
ACTCCATCCCTATTTATTGAATAATGCAGTAAGAATTTAATCGGTTTGGCAATGATGCCGAGCTTGAAAGCTTTTTATTA
TTTAATTGAGAGGACACAAC

Downstream 100 bases:

>100_bases
TTATTTAAAGGGCGGGAATTGAACATTCCCGCTTTTTACGTTTGTTGCCACTAATGTTTATTGATACAATAGAAAAAGTT
GTGAGCAAACGTTTGCAAAA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSVIKMTDLELAGKRVFIRADLNVPVKDGKVTSDARILASLPTIKLCLEAGAKVMVTSHLGRPTEGEYNEEFSLAPVVNY
LNDALDCDVKLAKDYLDGLELNAGELVVLENVRFNKGEKKNEEALSKKYAALCDIFVMDAFGTAHRAQASTHGVGMNAPI
ACAGPLLAAELEALGKAMDNPARPLVAIVGGSKVSTKLTVLESLSKIADQLVVGGGIANTFIAAQGHNVGKSLYEADLVE
TAKKLMVECAIPVATDVACAKAFDENAEAEIKNVADVQDDDMIFDLGPDSTAVLADIIKNAKTILWNGPVGVFEFKNFEA
GTAGISKAIADSEGFSVAGGGDTLAAIDKFGIKADVSYISTGGGAFLEFVEGKVLPAVAMLEERAKA

Sequences:

>Translated_387_residues
MSVIKMTDLELAGKRVFIRADLNVPVKDGKVTSDARILASLPTIKLCLEAGAKVMVTSHLGRPTEGEYNEEFSLAPVVNY
LNDALDCDVKLAKDYLDGLELNAGELVVLENVRFNKGEKKNEEALSKKYAALCDIFVMDAFGTAHRAQASTHGVGMNAPI
ACAGPLLAAELEALGKAMDNPARPLVAIVGGSKVSTKLTVLESLSKIADQLVVGGGIANTFIAAQGHNVGKSLYEADLVE
TAKKLMVECAIPVATDVACAKAFDENAEAEIKNVADVQDDDMIFDLGPDSTAVLADIIKNAKTILWNGPVGVFEFKNFEA
GTAGISKAIADSEGFSVAGGGDTLAAIDKFGIKADVSYISTGGGAFLEFVEGKVLPAVAMLEERAKA
>Mature_386_residues
SVIKMTDLELAGKRVFIRADLNVPVKDGKVTSDARILASLPTIKLCLEAGAKVMVTSHLGRPTEGEYNEEFSLAPVVNYL
NDALDCDVKLAKDYLDGLELNAGELVVLENVRFNKGEKKNEEALSKKYAALCDIFVMDAFGTAHRAQASTHGVGMNAPIA
CAGPLLAAELEALGKAMDNPARPLVAIVGGSKVSTKLTVLESLSKIADQLVVGGGIANTFIAAQGHNVGKSLYEADLVET
AKKLMVECAIPVATDVACAKAFDENAEAEIKNVADVQDDDMIFDLGPDSTAVLADIIKNAKTILWNGPVGVFEFKNFEAG
TAGISKAIADSEGFSVAGGGDTLAAIDKFGIKADVSYISTGGGAFLEFVEGKVLPAVAMLEERAKA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=413, Percent_Identity=38.2566585956416, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI4505763, Length=413, Percent_Identity=38.498789346247, Blast_Score=252, Evalue=4e-67,
Organism=Escherichia coli, GI1789294, Length=386, Percent_Identity=78.2383419689119, Blast_Score=606, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI17508823, Length=420, Percent_Identity=40.4761904761905, Blast_Score=271, Evalue=6e-73,
Organism=Saccharomyces cerevisiae, GI6319857, Length=419, Percent_Identity=39.8568019093079, Blast_Score=268, Evalue=1e-72,
Organism=Drosophila melanogaster, GI17136394, Length=413, Percent_Identity=41.6464891041162, Blast_Score=260, Evalue=9e-70,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=38.0722891566265, Blast_Score=238, Evalue=4e-63,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 40626; Mature: 40495

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIKMTDLELAGKRVFIRADLNVPVKDGKVTSDARILASLPTIKLCLEAGAKVMVTSHL
CCEEEECCCEECCCEEEEEECCCCCCCCCCCCCCCHHHHHCCHHHHHHHCCCEEEEEECC
GRPTEGEYNEEFSLAPVVNYLNDALDCDVKLAKDYLDGLELNAGELVVLENVRFNKGEKK
CCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCC
NEEALSKKYAALCDIFVMDAFGTAHRAQASTHGVGMNAPIACAGPLLAAELEALGKAMDN
CHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHCC
PARPLVAIVGGSKVSTKLTVLESLSKIADQLVVGGGIANTFIAAQGHNVGKSLYEADLVE
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCHHHHEEEECCCCHHHHHHHHHHHH
TAKKLMVECAIPVATDVACAKAFDENAEAEIKNVADVQDDDMIFDLGPDSTAVLADIIKN
HHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHC
AKTILWNGPVGVFEFKNFEAGTAGISKAIADSEGFSVAGGGDTLAAIDKFGIKADVSYIS
CCEEEECCCCCEEEECCCCCCHHHHHHHHCCCCCCEEECCCCHHHHHHHCCCEEECCEEE
TGGGAFLEFVEGKVLPAVAMLEERAKA
CCCHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVIKMTDLELAGKRVFIRADLNVPVKDGKVTSDARILASLPTIKLCLEAGAKVMVTSHL
CEEEECCCEECCCEEEEEECCCCCCCCCCCCCCCHHHHHCCHHHHHHHCCCEEEEEECC
GRPTEGEYNEEFSLAPVVNYLNDALDCDVKLAKDYLDGLELNAGELVVLENVRFNKGEKK
CCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCC
NEEALSKKYAALCDIFVMDAFGTAHRAQASTHGVGMNAPIACAGPLLAAELEALGKAMDN
CHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHCC
PARPLVAIVGGSKVSTKLTVLESLSKIADQLVVGGGIANTFIAAQGHNVGKSLYEADLVE
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCHHHHEEEECCCCHHHHHHHHHHHH
TAKKLMVECAIPVATDVACAKAFDENAEAEIKNVADVQDDDMIFDLGPDSTAVLADIIKN
HHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHC
AKTILWNGPVGVFEFKNFEAGTAGISKAIADSEGFSVAGGGDTLAAIDKFGIKADVSYIS
CCEEEECCCCCEEEECCCCCCHHHHHHHHCCCCCCEEECCCCHHHHHHHCCCEEECCEEE
TGGGAFLEFVEGKVLPAVAMLEERAKA
CCCHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA