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The map label for this gene is metK [H]

Identifier: 209694145

GI number: 209694145

Start: 604227

End: 605381

Strand: Reverse

Name: metK [H]

Synonym: VSAL_I0552

Alternate gene names: 209694145

Gene position: 605381-604227 (Counterclockwise)

Preceding gene: 209694146

Following gene: 209694144

Centisome position: 18.21

GC content: 42.6

Gene sequence:

>1155_bases
ATGGCTAAACACCTATTTACCTCTGAATCAGTATCAGAAGGTCATCCAGATAAAATTGCCGATCAAATTTCAGATGCCGT
ACTTGATGCGATCTTAGAACAAGATCCAAAAGCACGTGTTGCTTGTGAAACGTACGTAAAAACTGGCATGGTTATGGTCG
GTGGTGAAGTAACAACTTCTGCATGGGTTGATATCGAAGAAATCACTCGTGAAACCGTTCGTGAAATTGGTTATGTTCAT
TCTGACATGGGCTTTGATGCTAACTCTTGTGCTGTATTAAACACAATTGGTAAGCAATCTCCTGATATCAACCAAGGTGT
TGATAAAGAAGATCCAAAAGAACAAGGCGCTGGCGACCAAGGCATCATGTTTGGTTACGCAACGAACGAAACACCAATTT
TAATGCCAGCACCGATTACTTACGCACACCTTCTTGTTAAACAACAAGCAGAAGTACGTAAAAGCGGAAAATTGGATTTC
TTACGCCCAGATGCAAAATCTCAAGTTACTTTTCAATACGACCAAGGAAAAATTGTTGGTATTGATGCGGTTGTACTTTC
AACTCAACACAGCGATTCAGTAACGACTGCGGATCTTCGTGAAGCAGTAATGGAAGAAATCATCAAACCAGTACTACCTG
CTGAATGGCTAAGTAAAGAAACTAAATTCTTTATTAACCCAACAGGTCGTTTTGTTATCGGTGGCCCAATGGGTGACTGT
GGCCTGACTGGTCGTAAAATCATCGTTGATACTTACGGCGGCGCAGCTCGTCACGGTGGCGGTGCATTCTCTGGTAAAGA
TCCATCAAAAGTTGACCGTTCAGCAGCATACGCAGCACGTTATGTAGCGAAAAACATCGTTGCAGCTGGCATGGCTGACC
GTTGTGAAATCCAACTGTCATACGCTATCGGTGTTGCTGATCCAACATCTATCATGATTGAAACGTTTGGTACTGAAAAA
GTATCTCACGACATCATCATTGAATCGGTTCGTCAGCACTTTGACTTACGTCCATATGGCCTACAAGAAATGCTTAACCT
TCTTCAACCAATCTACAAGAAGACAGCAGCATACGGTCACTTCGGTCGTGAAGAATTCCCTTGGGAAGCGACTGATAAAG
CAGAAATCCTTCGTGATTTCGCTGGTATTAAGTAA

Upstream 100 bases:

>100_bases
GCCGTCTAGATGGAAGAACACCTACACTGTTAAACGTATAGACTTATATATACTGTGGGTATTTTCTAAAAATTTATTAC
CTATAGAAGTGGAGCTCATC

Downstream 100 bases:

>100_bases
TTCAAGACTTAAAAACTCTCTAAAAAGGAAGCTTCGGCTTCCTTTTTTGTTTTCTAGCCCCATATACTCTTTATAGTTAA
AAGCGTTAATGTGATATTCA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRETVREIGYVH
SDMGFDANSCAVLNTIGKQSPDINQGVDKEDPKEQGAGDQGIMFGYATNETPILMPAPITYAHLLVKQQAEVRKSGKLDF
LRPDAKSQVTFQYDQGKIVGIDAVVLSTQHSDSVTTADLREAVMEEIIKPVLPAEWLSKETKFFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLSYAIGVADPTSIMIETFGTEK
VSHDIIIESVRQHFDLRPYGLQEMLNLLQPIYKKTAAYGHFGREEFPWEATDKAEILRDFAGIK

Sequences:

>Translated_384_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRETVREIGYVH
SDMGFDANSCAVLNTIGKQSPDINQGVDKEDPKEQGAGDQGIMFGYATNETPILMPAPITYAHLLVKQQAEVRKSGKLDF
LRPDAKSQVTFQYDQGKIVGIDAVVLSTQHSDSVTTADLREAVMEEIIKPVLPAEWLSKETKFFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLSYAIGVADPTSIMIETFGTEK
VSHDIIIESVRQHFDLRPYGLQEMLNLLQPIYKKTAAYGHFGREEFPWEATDKAEILRDFAGIK
>Mature_383_residues
AKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRETVREIGYVHS
DMGFDANSCAVLNTIGKQSPDINQGVDKEDPKEQGAGDQGIMFGYATNETPILMPAPITYAHLLVKQQAEVRKSGKLDFL
RPDAKSQVTFQYDQGKIVGIDAVVLSTQHSDSVTTADLREAVMEEIIKPVLPAEWLSKETKFFINPTGRFVIGGPMGDCG
LTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLSYAIGVADPTSIMIETFGTEKV
SHDIIIESVRQHFDLRPYGLQEMLNLLQPIYKKTAAYGHFGREEFPWEATDKAEILRDFAGIK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=374, Percent_Identity=56.1497326203209, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=56.1497326203209, Blast_Score=404, Evalue=1e-113,
Organism=Escherichia coli, GI1789311, Length=384, Percent_Identity=85.15625, Blast_Score=687, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17538498, Length=383, Percent_Identity=54.3080939947781, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17538494, Length=379, Percent_Identity=54.353562005277, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25145633, Length=383, Percent_Identity=53.7859007832898, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17551082, Length=373, Percent_Identity=54.4235924932976, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI32565905, Length=379, Percent_Identity=49.0765171503958, Blast_Score=337, Evalue=6e-93,
Organism=Caenorhabditis elegans, GI32565911, Length=336, Percent_Identity=50.5952380952381, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI25145635, Length=336, Percent_Identity=50, Blast_Score=321, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI32565903, Length=305, Percent_Identity=50.8196721311475, Blast_Score=293, Evalue=8e-80,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=62.6506024096386, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=46, Percent_Identity=73.9130434782609, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=377, Percent_Identity=57.8249336870027, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6323209, Length=370, Percent_Identity=58.3783783783784, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI45552159, Length=380, Percent_Identity=54.7368421052632, Blast_Score=383, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580529, Length=380, Percent_Identity=54.7368421052632, Blast_Score=383, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580537, Length=380, Percent_Identity=54.7368421052632, Blast_Score=383, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580533, Length=380, Percent_Identity=54.7368421052632, Blast_Score=383, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580535, Length=380, Percent_Identity=54.7368421052632, Blast_Score=383, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580531, Length=380, Percent_Identity=54.7368421052632, Blast_Score=383, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580527, Length=380, Percent_Identity=54.2105263157895, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580525, Length=380, Percent_Identity=54.2105263157895, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580541, Length=379, Percent_Identity=51.9788918205805, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580539, Length=120, Percent_Identity=55.8333333333333, Blast_Score=131, Evalue=8e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41940; Mature: 41809

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCCH
AWVDIEEITRETVREIGYVHSDMGFDANSCAVLNTIGKQSPDINQGVDKEDPKEQGAGDQ
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCC
GIMFGYATNETPILMPAPITYAHLLVKQQAEVRKSGKLDFLRPDAKSQVTFQYDQGKIVG
CEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEECCCEEEE
IDAVVLSTQHSDSVTTADLREAVMEEIIKPVLPAEWLSKETKFFINPTGRFVIGGPMGDC
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVADPTSIMIETFGTEKVSHDIIIESVRQHFDLRPYGLQEMLNLLQPIYKKTAAYGH
EEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
FGREEFPWEATDKAEILRDFAGIK
CCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
AKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCCH
AWVDIEEITRETVREIGYVHSDMGFDANSCAVLNTIGKQSPDINQGVDKEDPKEQGAGDQ
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCC
GIMFGYATNETPILMPAPITYAHLLVKQQAEVRKSGKLDFLRPDAKSQVTFQYDQGKIVG
CEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEECCCEEEE
IDAVVLSTQHSDSVTTADLREAVMEEIIKPVLPAEWLSKETKFFINPTGRFVIGGPMGDC
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVADPTSIMIETFGTEKVSHDIIIESVRQHFDLRPYGLQEMLNLLQPIYKKTAAYGH
EEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
FGREEFPWEATDKAEILRDFAGIK
CCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA