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The map label for this gene is gshB [H]

Identifier: 209694141

GI number: 209694141

Start: 601042

End: 602028

Strand: Reverse

Name: gshB [H]

Synonym: VSAL_I0548

Alternate gene names: 209694141

Gene position: 602028-601042 (Counterclockwise)

Preceding gene: 209694142

Following gene: 209694140

Centisome position: 18.11

GC content: 41.34

Gene sequence:

>987_bases
TTGGCGATCTTGGCTAACTGGAGAAACACAATGATCAAACTCGGTATCGTAATGGATCCTATTTCATCCATTAACATTAA
AAAAGACTCTAGCTTTGCCATGATGCTTGAAGCTCAAAAACGCGGCTGGGAAATCCATTACATGGAAATGGCCGATCTTC
ACCTAGACCAAGGTAAAGCAGTTGCCGATACTAAAGTTGTTGAACTAAAAGAAGATCCTACTGGTTGGTACGAATTCAAA
TCAGAGCAGACGATTGATCTTGCTGAACTTGATGCCGTCTTAATGCGTAAAGATCCTCCGTTTGATACAGAATACATTTA
CGCGACCTACATTTTAGAGCGCGCAGAAATTCAAGGTGCTTTAATTGTAAACAAACCACAAAGCTTACGTGATTGTAATG
AAAAACTGTTTACCGCTTGGTTCCCAGAACTGACACCAACAACGCTAGTTACTCGTAAATCAGACAAAATTAAAGCGTTC
CGCGAAAAACACGGTGACATTATTCTTAAGCCGCTTGATGGTATGGGTGGTGCATCAATTTTCCGTGTGAAAGCAGGCGA
TCCAAACGTGTCGGTTATCATCGAAACACTCACTAACCACGAACAAAACTATTGCATGGCACAGACGTTTGTTCCTGATA
TTAGTAATGGTGATAAACGTATTCTAGTAGTTGATGGTGAACCAATGCCTTATTGCTTGGCACGTATCCCTGCAAAAGGC
GAAACTCGTGGTAATTTAGCCGCTGGTGGTACCGGTGAAGCTAGACCACTCAGTGAAACCGATTGGGCAATTGCAAATGC
GGTTGCTCCAACCCTAAAAGAAAAAGGACTTATTTTTGTTGGCCTTGATGTTATAGGCGACAAATTAACCGAAATTAACG
TTACCAGTCCTACGTGTATTAAAGAGATCGAAGCCGCTTTTGATATCTCAATTACAGGAAAATTAATGGATGCAATAGAG
CGTCGCATTAATGAGAAGTCAGCTTAA

Upstream 100 bases:

>100_bases
ATGACAGAACAATATCAATTTGAAGAAACGTTGCTTGGTCCTCGGGTTTTAAGAACCGAAACCGCAGCACTTACTGCAAT
TACTGCCTTACAAGTTCGTT

Downstream 100 bases:

>100_bases
GTAAAATCACCTAAGCTAAAGATAAGCAGTGGGAAATCCCCCACTGTTTTATCATACGATTTTAGGAGGCATTATGGATT
TAAAAAACCATTTTCTGGTT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MAILANWRNTMIKLGIVMDPISSINIKKDSSFAMMLEAQKRGWEIHYMEMADLHLDQGKAVADTKVVELKEDPTGWYEFK
SEQTIDLAELDAVLMRKDPPFDTEYIYATYILERAEIQGALIVNKPQSLRDCNEKLFTAWFPELTPTTLVTRKSDKIKAF
REKHGDIILKPLDGMGGASIFRVKAGDPNVSVIIETLTNHEQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKG
ETRGNLAAGGTGEARPLSETDWAIANAVAPTLKEKGLIFVGLDVIGDKLTEINVTSPTCIKEIEAAFDISITGKLMDAIE
RRINEKSA

Sequences:

>Translated_328_residues
MAILANWRNTMIKLGIVMDPISSINIKKDSSFAMMLEAQKRGWEIHYMEMADLHLDQGKAVADTKVVELKEDPTGWYEFK
SEQTIDLAELDAVLMRKDPPFDTEYIYATYILERAEIQGALIVNKPQSLRDCNEKLFTAWFPELTPTTLVTRKSDKIKAF
REKHGDIILKPLDGMGGASIFRVKAGDPNVSVIIETLTNHEQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKG
ETRGNLAAGGTGEARPLSETDWAIANAVAPTLKEKGLIFVGLDVIGDKLTEINVTSPTCIKEIEAAFDISITGKLMDAIE
RRINEKSA
>Mature_327_residues
AILANWRNTMIKLGIVMDPISSINIKKDSSFAMMLEAQKRGWEIHYMEMADLHLDQGKAVADTKVVELKEDPTGWYEFKS
EQTIDLAELDAVLMRKDPPFDTEYIYATYILERAEIQGALIVNKPQSLRDCNEKLFTAWFPELTPTTLVTRKSDKIKAFR
EKHGDIILKPLDGMGGASIFRVKAGDPNVSVIIETLTNHEQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGE
TRGNLAAGGTGEARPLSETDWAIANAVAPTLKEKGLIFVGLDVIGDKLTEINVTSPTCIKEIEAAFDISITGKLMDAIER
RINEKSA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=316, Percent_Identity=72.7848101265823, Blast_Score=490, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 36450; Mature: 36319

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILANWRNTMIKLGIVMDPISSINIKKDSSFAMMLEAQKRGWEIHYMEMADLHLDQGKA
CEEEECCCCCEEEEEEEECCCCCCEEECCCCEEEEEEHHHCCCEEEEEEEEEEECCCCCE
VADTKVVELKEDPTGWYEFKSEQTIDLAELDAVLMRKDPPFDTEYIYATYILERAEIQGA
EECEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEEEEHHHCCEE
LIVNKPQSLRDCNEKLFTAWFPELTPTTLVTRKSDKIKAFREKHGDIILKPLDGMGGASI
EEEECCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCCCEEEEECCCCCCCEE
FRVKAGDPNVSVIIETLTNHEQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKG
EEEECCCCCCEEEEEEHHCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCHHHHCCCCCC
ETRGNLAAGGTGEARPLSETDWAIANAVAPTLKEKGLIFVGLDVIGDKLTEINVTSPTCI
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEHHHHCCCEEEEECCCCHHH
KEIEAAFDISITGKLMDAIERRINEKSA
HHHHHHEEEEEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AILANWRNTMIKLGIVMDPISSINIKKDSSFAMMLEAQKRGWEIHYMEMADLHLDQGKA
EEEECCCCCEEEEEEEECCCCCCEEECCCCEEEEEEHHHCCCEEEEEEEEEEECCCCCE
VADTKVVELKEDPTGWYEFKSEQTIDLAELDAVLMRKDPPFDTEYIYATYILERAEIQGA
EECEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEEEEHHHCCEE
LIVNKPQSLRDCNEKLFTAWFPELTPTTLVTRKSDKIKAFREKHGDIILKPLDGMGGASI
EEEECCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCCCEEEEECCCCCCCEE
FRVKAGDPNVSVIIETLTNHEQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKG
EEEECCCCCCEEEEEEHHCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCHHHHCCCCCC
ETRGNLAAGGTGEARPLSETDWAIANAVAPTLKEKGLIFVGLDVIGDKLTEINVTSPTCI
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEHHHHCCCEEEEECCCCHHH
KEIEAAFDISITGKLMDAIERRINEKSA
HHHHHHEEEEEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]