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The map label for this gene is rsmE [H]

Identifier: 209694142

GI number: 209694142

Start: 602012

End: 602743

Strand: Reverse

Name: rsmE [H]

Synonym: VSAL_I0549

Alternate gene names: 209694142

Gene position: 602743-602012 (Counterclockwise)

Preceding gene: 209694143

Following gene: 209694141

Centisome position: 18.13

GC content: 39.21

Gene sequence:

>732_bases
ATGAGAATACCAAGAATCCATCATCCAGAACTACTTCCTTCACAAGGTAAAGTAAACTTAAGCGATGATGCCGCCAATCA
TGTTGGCCGTGTAATGCGAATGAAAGAAGGCGAAGCTGTATTATTATTTGATGGCAGCGGCGCTGAATTTCCTGCAGTGA
TCACTTCTTCATCAAAGAAAAGTGTAGAAGTTGAGATCAGTGAACGTATTGATCACAGCAGTGAATCACCATTAAATTTA
CATTTAGGCCAAGTGGTTTCTCGTGGTGATAAAATGGAATTCACCATTCAAAAATCCGTGGAATTAGGCGTAAATATCAT
TACTCCTTTAATTTCAGAACGTTGTGGTGTCAAATTAAACAAAGAACGTTTTGAAAAGAAACTCGACCAATGGCACAAAA
TTGCAATAGCCGCTTGTGAGCAATGTGGACGTAATACTATTCCTGAAATTCGTCCAATTATGTCATTAGAAGAATGGTGT
GCTGAAGAATATGAAGGACTCAAATTAAACCTTCATCCAAGAGCCAAATATTCGATTAACACCCTACCGGAACCTGTTAC
TAATGTTCGTTTGTTGATTGGTCCAGAAGGCGGTTTGTCGTCTGAAGAAATTACTATGACAGAACAATATCAATTTGAAG
AAACGTTGCTTGGTCCTCGGGTTTTAAGAACCGAAACCGCAGCACTTACTGCAATTACTGCCTTACAAGTTCGTTTTGGC
GATCTTGGCTAA

Upstream 100 bases:

>100_bases
TTATAAAGCATGCACTAAATAAATTAGACAGAGCTAGAGGTTGAAAAAGTACATTTTAACCTCCATCCTTAAATGTAAGA
TAGTTAAAGAAAAGTAGACC

Downstream 100 bases:

>100_bases
CTGGAGAAACACAATGATCAAACTCGGTATCGTAATGGATCCTATTTCATCCATTAACATTAAAAAAGACTCTAGCTTTG
CCATGATGCTTGAAGCTCAA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRIPRIHHPELLPSQGKVNLSDDAANHVGRVMRMKEGEAVLLFDGSGAEFPAVITSSSKKSVEVEISERIDHSSESPLNL
HLGQVVSRGDKMEFTIQKSVELGVNIITPLISERCGVKLNKERFEKKLDQWHKIAIAACEQCGRNTIPEIRPIMSLEEWC
AEEYEGLKLNLHPRAKYSINTLPEPVTNVRLLIGPEGGLSSEEITMTEQYQFEETLLGPRVLRTETAALTAITALQVRFG
DLG

Sequences:

>Translated_243_residues
MRIPRIHHPELLPSQGKVNLSDDAANHVGRVMRMKEGEAVLLFDGSGAEFPAVITSSSKKSVEVEISERIDHSSESPLNL
HLGQVVSRGDKMEFTIQKSVELGVNIITPLISERCGVKLNKERFEKKLDQWHKIAIAACEQCGRNTIPEIRPIMSLEEWC
AEEYEGLKLNLHPRAKYSINTLPEPVTNVRLLIGPEGGLSSEEITMTEQYQFEETLLGPRVLRTETAALTAITALQVRFG
DLG
>Mature_243_residues
MRIPRIHHPELLPSQGKVNLSDDAANHVGRVMRMKEGEAVLLFDGSGAEFPAVITSSSKKSVEVEISERIDHSSESPLNL
HLGQVVSRGDKMEFTIQKSVELGVNIITPLISERCGVKLNKERFEKKLDQWHKIAIAACEQCGRNTIPEIRPIMSLEEWC
AEEYEGLKLNLHPRAKYSINTLPEPVTNVRLLIGPEGGLSSEEITMTEQYQFEETLLGPRVLRTETAALTAITALQVRFG
DLG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=72.4279835390947, Blast_Score=361, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27079; Mature: 27079

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPRIHHPELLPSQGKVNLSDDAANHVGRVMRMKEGEAVLLFDGSGAEFPAVITSSSKK
CCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHEECCCCEEEEECCCCCCCCEEEECCCCC
SVEVEISERIDHSSESPLNLHLGQVVSRGDKMEFTIQKSVELGVNIITPLISERCGVKLN
EEEEEEHHHCCCCCCCCCEEEHHHHHCCCCCEEEEEECHHHCCHHHHHHHHHHHCCCEEC
KERFEKKLDQWHKIAIAACEQCGRNTIPEIRPIMSLEEWCAEEYEGLKLNLHPRAKYSIN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEECC
TLPEPVTNVRLLIGPEGGLSSEEITMTEQYQFEETLLGPRVLRTETAALTAITALQVRFG
CCCCCCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECC
DLG
CCC
>Mature Secondary Structure
MRIPRIHHPELLPSQGKVNLSDDAANHVGRVMRMKEGEAVLLFDGSGAEFPAVITSSSKK
CCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHEECCCCEEEEECCCCCCCCEEEECCCCC
SVEVEISERIDHSSESPLNLHLGQVVSRGDKMEFTIQKSVELGVNIITPLISERCGVKLN
EEEEEEHHHCCCCCCCCCEEEHHHHHCCCCCEEEEEECHHHCCHHHHHHHHHHHCCCEEC
KERFEKKLDQWHKIAIAACEQCGRNTIPEIRPIMSLEEWCAEEYEGLKLNLHPRAKYSIN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEECC
TLPEPVTNVRLLIGPEGGLSSEEITMTEQYQFEETLLGPRVLRTETAALTAITALQVRFG
CCCCCCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECC
DLG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]