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The map label for this gene is pilU [H]

Identifier: 209694138

GI number: 209694138

Start: 598773

End: 599879

Strand: Direct

Name: pilU [H]

Synonym: VSAL_I0545

Alternate gene names: 209694138

Gene position: 598773-599879 (Clockwise)

Preceding gene: 209694137

Following gene: 209694147

Centisome position: 18.01

GC content: 41.1

Gene sequence:

>1107_bases
ATGAATTTGGATGATTTATTGAATCAGATGGTGAAGCAGAAAGCCTCTGATATTTATATTACGGTGGATGCACCGTGTTT
ATTACGTGTTGATGGCGTTTTACAAGCGACAGGGGCAAAGCAAACGGTAGAGTCTGTAACCGCCCTGTTGGCTGAGATGA
TGAATGATGATCGAGAAAAAGAATTTACTCAAACATTAGAAGCCAATTTTGCCATCGTAAAAGAGAGTGGTCGTTTTCGT
GTGAGTGCATTTTGGCAGCGTGAACAACCGGGGGCCGTGATCCGTAGAATTGAAACTAAAATCCCATCCATGGATTCATT
GTATTTACCGAGTATCATGAAAGACTTGGCGGTAGCTAAACGAGGATTGGTTCTAGTTGTGGGAGCGACGGGATCAGGAA
AATCGACCACAATGGCTGCGATGACTGGGTACCGGAATGATCATAAATCGGGTCATATTTTGACGGTGGAAGATCCGATT
GAATTTGTTCATCCACATAATAAATGCATCGTGACTCAACGTGAAGTTGGGCTAGATACAGACAGTTATGAAGTTGCTTT
AAAAAATTCATTACGCCAAGCGCCCGATATGATTTTGATTGGTGAAATTCGAACCAGAGAAACGATGAAGTACGCGATGA
TGTTTGCTGAGACTGGGCATTTGTGTATGGCAACGTTACACGCAAACAATGCAAACCAAGCATTAGAGCGTATTTTACAT
TTGGTTCCAAAAGAGAATAAAGAGCAATTTTTATTTGATCTCTCAATGAATTTAAAAGGTGTTATTGCTCAACAATTGGT
TCCTGATGCTCAGGGAAATGGGCGTCACGGTGTGTTTGAAGTCTTACTCAATACTCCCCGAATCGCCGATTTAATTCGTA
GAGGTGATCTGCATGAATTAAAAGGGACAATGACACGCTCTCGTGAATCAGGAATGCAGACGTTTGATCAATGCTTATTT
GATTTAGTAATTGATGGCAAGATTACAGAAACAGAAGCGCTGCATAGTGCGGATTCTGCAAATGACTTAAGGCTGATGCT
GAAGACTAAGCGTGGGGATTCATTTACCACATCAAAATTTGATAATGTGTCGATATCGTTAGATTAA

Upstream 100 bases:

>100_bases
AAACCATGGAGCAAAATGCAAAGAAAATTATTGCTCAAGGGTTAGTTGACCCTGATGAAGTTGCAAAGAAAGTTGAAATA
ACCAGTATGGGTTTTTAATC

Downstream 100 bases:

>100_bases
AAGCAGGATTCAGGACGCTTTGCGTTTAGAATTCAGAGGTGTGTGCGGTGAGTGTTACGCTCACAACGAGTACCTCTGAA
CCCTGCTTTTATGTCTTTGG

Product: twitching motility protein PilU, type II/IV secretion system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MNLDDLLNQMVKQKASDIYITVDAPCLLRVDGVLQATGAKQTVESVTALLAEMMNDDREKEFTQTLEANFAIVKESGRFR
VSAFWQREQPGAVIRRIETKIPSMDSLYLPSIMKDLAVAKRGLVLVVGATGSGKSTTMAAMTGYRNDHKSGHILTVEDPI
EFVHPHNKCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRTRETMKYAMMFAETGHLCMATLHANNANQALERILH
LVPKENKEQFLFDLSMNLKGVIAQQLVPDAQGNGRHGVFEVLLNTPRIADLIRRGDLHELKGTMTRSRESGMQTFDQCLF
DLVIDGKITETEALHSADSANDLRLMLKTKRGDSFTTSKFDNVSISLD

Sequences:

>Translated_368_residues
MNLDDLLNQMVKQKASDIYITVDAPCLLRVDGVLQATGAKQTVESVTALLAEMMNDDREKEFTQTLEANFAIVKESGRFR
VSAFWQREQPGAVIRRIETKIPSMDSLYLPSIMKDLAVAKRGLVLVVGATGSGKSTTMAAMTGYRNDHKSGHILTVEDPI
EFVHPHNKCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRTRETMKYAMMFAETGHLCMATLHANNANQALERILH
LVPKENKEQFLFDLSMNLKGVIAQQLVPDAQGNGRHGVFEVLLNTPRIADLIRRGDLHELKGTMTRSRESGMQTFDQCLF
DLVIDGKITETEALHSADSANDLRLMLKTKRGDSFTTSKFDNVSISLD
>Mature_368_residues
MNLDDLLNQMVKQKASDIYITVDAPCLLRVDGVLQATGAKQTVESVTALLAEMMNDDREKEFTQTLEANFAIVKESGRFR
VSAFWQREQPGAVIRRIETKIPSMDSLYLPSIMKDLAVAKRGLVLVVGATGSGKSTTMAAMTGYRNDHKSGHILTVEDPI
EFVHPHNKCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRTRETMKYAMMFAETGHLCMATLHANNANQALERILH
LVPKENKEQFLFDLSMNLKGVIAQQLVPDAQGNGRHGVFEVLLNTPRIADLIRRGDLHELKGTMTRSRESGMQTFDQCLF
DLVIDGKITETEALHSADSANDLRLMLKTKRGDSFTTSKFDNVSISLD

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=329, Percent_Identity=37.9939209726444, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1789723, Length=280, Percent_Identity=32.8571428571429, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1786296, Length=373, Percent_Identity=30.2949061662198, Blast_Score=95, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41028; Mature: 41028

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDDLLNQMVKQKASDIYITVDAPCLLRVDGVLQATGAKQTVESVTALLAEMMNDDREK
CCHHHHHHHHHHHCCCCEEEEECCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHCCHHHH
EFTQTLEANFAIVKESGRFRVSAFWQREQPGAVIRRIETKIPSMDSLYLPSIMKDLAVAK
HHHHHHHCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
RGLVLVVGATGSGKSTTMAAMTGYRNDHKSGHILTVEDPIEFVHPHNKCIVTQREVGLDT
CCEEEEEECCCCCCCCCHHHHHCCCCCCCCCCEEEEECCHHHHCCCCEEEEEEHHCCCCC
DSYEVALKNSLRQAPDMILIGEIRTRETMKYAMMFAETGHLCMATLHANNANQALERILH
CCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHH
LVPKENKEQFLFDLSMNLKGVIAQQLVPDAQGNGRHGVFEVLLNTPRIADLIRRGDLHEL
HCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHCCCHHHH
KGTMTRSRESGMQTFDQCLFDLVIDGKITETEALHSADSANDLRLMLKTKRGDSFTTSKF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCC
DNVSISLD
CCEEEEEC
>Mature Secondary Structure
MNLDDLLNQMVKQKASDIYITVDAPCLLRVDGVLQATGAKQTVESVTALLAEMMNDDREK
CCHHHHHHHHHHHCCCCEEEEECCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHCCHHHH
EFTQTLEANFAIVKESGRFRVSAFWQREQPGAVIRRIETKIPSMDSLYLPSIMKDLAVAK
HHHHHHHCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
RGLVLVVGATGSGKSTTMAAMTGYRNDHKSGHILTVEDPIEFVHPHNKCIVTQREVGLDT
CCEEEEEECCCCCCCCCHHHHHCCCCCCCCCCEEEEECCHHHHCCCCEEEEEEHHCCCCC
DSYEVALKNSLRQAPDMILIGEIRTRETMKYAMMFAETGHLCMATLHANNANQALERILH
CCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHH
LVPKENKEQFLFDLSMNLKGVIAQQLVPDAQGNGRHGVFEVLLNTPRIADLIRRGDLHEL
HCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHCCCHHHH
KGTMTRSRESGMQTFDQCLFDLVIDGKITETEALHSADSANDLRLMLKTKRGDSFTTSKF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCC
DNVSISLD
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]