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The map label for this gene is mutY [H]

Identifier: 209694127

GI number: 209694127

Start: 589835

End: 590887

Strand: Reverse

Name: mutY [H]

Synonym: VSAL_I0534

Alternate gene names: 209694127

Gene position: 590887-589835 (Counterclockwise)

Preceding gene: 209694130

Following gene: 209694126

Centisome position: 17.77

GC content: 42.17

Gene sequence:

>1053_bases
ATGACTTCTTTTTCTCACGCCATCTTAGAATGGTACGATAATTACGGCCGTAAGACGTTGCCTTGGCAATTAGAAAAAAC
ACCTTATAAAGTATGGTTATCCGAAATCATGCTGCAACAAACTCAAGTCACTACCGTTATTCCTTATTTTGAACGCTTCA
TGACTCGCTTTCCTACGGTAATTGACCTAGCGAACGCCGATCAAGATGAAGTTCTTCATTTATGGACTGGTCTTGGTTAT
TACGCCCGTGCTCGAAACTTACATAAAACCGCACAGATCATCGCAGAGCAATATAATGGCCGATTTCCTGAAACCATTGA
TGAAGTGATCGCTTTACCCGGAATTGGACGATCTACCGCTGGCGCCGTACTTTCCTTGTCGCTTAAACAACGTCATCCCA
TTTTAGATGGTAATGTAAAGCGAACCTTATCTCGCTGCTTTGCTATTGAAGGATGGTCAGGGAAGAAATCTGTCGAAAAT
GCCATGTGGGAAATAGCAGAAGAACATACACCAGAATTAGGAGTCGAACGCTACAATCAAGCCATGATGGATATGGGGGC
GATTGTCTGTACTCGTTCTAAGCCTAAGTGTGAGATTTGCCCAGTGAATGATCTTTGCCAAGCAAAAGCACAAGATCGCC
AATTGGATTTCCCCACCAAAAAACCGAAGAAAGAAAAACCAATGAAAGAAGCTTGGTTTGCCATTTATTATCACGATGGT
GAAGTTTGGTTAGAGCAAAGACCTCAGACTGGAATTTGGGGCGGGCTTTTTTGCTTTCCTGAACAACCAGAGAATACCTT
AGTTGATTTATCTGTTCGCTATGGTGTCGAAATAAAATCCACTCAACAATTAATTGCTTTTCGTCATACCTTTAGCCATT
ACCACTTAGATATCACCCCTGTGCTTATCACTCTGGTGAAGAAACCGAACATGGTGATGGAAGGAACACGCGGAGTTTGG
TATAACTTATCTCAACCAATGACGGTAGGATTAGCAGCACCAGTACAAAAACTATTGGATGCCCTTCCGTACGAAATTTC
TAATGGAGAATAA

Upstream 100 bases:

>100_bases
TTTGAATACTTGTATGAATACCCCTATTTTCTATCGGGTGTACAAATTCATCACAGTATGATGCAATCCCCACCATTATA
AAAAAGCAATCGAGCATGTC

Downstream 100 bases:

>100_bases
CCATGAGTCGCACTGTATTTTGCGTTCTTTTAAATAAAGAAGCCGATGGCCTAGATTTTCAACTTTACCCAGGTGAACTA
GGTAAACGTATTTTTGATAA

Product: A/G-specific adenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MTSFSHAILEWYDNYGRKTLPWQLEKTPYKVWLSEIMLQQTQVTTVIPYFERFMTRFPTVIDLANADQDEVLHLWTGLGY
YARARNLHKTAQIIAEQYNGRFPETIDEVIALPGIGRSTAGAVLSLSLKQRHPILDGNVKRTLSRCFAIEGWSGKKSVEN
AMWEIAEEHTPELGVERYNQAMMDMGAIVCTRSKPKCEICPVNDLCQAKAQDRQLDFPTKKPKKEKPMKEAWFAIYYHDG
EVWLEQRPQTGIWGGLFCFPEQPENTLVDLSVRYGVEIKSTQQLIAFRHTFSHYHLDITPVLITLVKKPNMVMEGTRGVW
YNLSQPMTVGLAAPVQKLLDALPYEISNGE

Sequences:

>Translated_350_residues
MTSFSHAILEWYDNYGRKTLPWQLEKTPYKVWLSEIMLQQTQVTTVIPYFERFMTRFPTVIDLANADQDEVLHLWTGLGY
YARARNLHKTAQIIAEQYNGRFPETIDEVIALPGIGRSTAGAVLSLSLKQRHPILDGNVKRTLSRCFAIEGWSGKKSVEN
AMWEIAEEHTPELGVERYNQAMMDMGAIVCTRSKPKCEICPVNDLCQAKAQDRQLDFPTKKPKKEKPMKEAWFAIYYHDG
EVWLEQRPQTGIWGGLFCFPEQPENTLVDLSVRYGVEIKSTQQLIAFRHTFSHYHLDITPVLITLVKKPNMVMEGTRGVW
YNLSQPMTVGLAAPVQKLLDALPYEISNGE
>Mature_349_residues
TSFSHAILEWYDNYGRKTLPWQLEKTPYKVWLSEIMLQQTQVTTVIPYFERFMTRFPTVIDLANADQDEVLHLWTGLGYY
ARARNLHKTAQIIAEQYNGRFPETIDEVIALPGIGRSTAGAVLSLSLKQRHPILDGNVKRTLSRCFAIEGWSGKKSVENA
MWEIAEEHTPELGVERYNQAMMDMGAIVCTRSKPKCEICPVNDLCQAKAQDRQLDFPTKKPKKEKPMKEAWFAIYYHDGE
VWLEQRPQTGIWGGLFCFPEQPENTLVDLSVRYGVEIKSTQQLIAFRHTFSHYHLDITPVLITLVKKPNMVMEGTRGVWY
NLSQPMTVGLAAPVQKLLDALPYEISNGE

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI115298648, Length=360, Percent_Identity=32.2222222222222, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI6912520, Length=360, Percent_Identity=32.2222222222222, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI115298654, Length=360, Percent_Identity=32.2222222222222, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI115298652, Length=360, Percent_Identity=32.2222222222222, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI115298650, Length=360, Percent_Identity=32.2222222222222, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI190358497, Length=360, Percent_Identity=32.2222222222222, Blast_Score=182, Evalue=3e-46,
Organism=Escherichia coli, GI1789331, Length=342, Percent_Identity=60.233918128655, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1787920, Length=132, Percent_Identity=30.3030303030303, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 40130; Mature: 39998

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFSHAILEWYDNYGRKTLPWQLEKTPYKVWLSEIMLQQTQVTTVIPYFERFMTRFPTV
CCCHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
IDLANADQDEVLHLWTGLGYYARARNLHKTAQIIAEQYNGRFPETIDEVIALPGIGRSTA
EEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCC
GAVLSLSLKQRHPILDGNVKRTLSRCFAIEGWSGKKSVENAMWEIAEEHTPELGVERYNQ
CCEEEEEHHHCCCCCCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
AMMDMGAIVCTRSKPKCEICPVNDLCQAKAQDRQLDFPTKKPKKEKPMKEAWFAIYYHDG
HHHHCCCEEEECCCCCEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHEEEEEEEECC
EVWLEQRPQTGIWGGLFCFPEQPENTLVDLSVRYGVEIKSTQQLIAFRHTFSHYHLDITP
EEEEECCCCCCCCCCEEECCCCCCCCEEEEEEEECEEEHHHHHHHHHHHHHHHEEEEHHH
VLITLVKKPNMVMEGTRGVWYNLSQPMTVGLAAPVQKLLDALPYEISNGE
HHHHHHCCCCEEEECCCCEEEECCCCCEEHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TSFSHAILEWYDNYGRKTLPWQLEKTPYKVWLSEIMLQQTQVTTVIPYFERFMTRFPTV
CCHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
IDLANADQDEVLHLWTGLGYYARARNLHKTAQIIAEQYNGRFPETIDEVIALPGIGRSTA
EEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCC
GAVLSLSLKQRHPILDGNVKRTLSRCFAIEGWSGKKSVENAMWEIAEEHTPELGVERYNQ
CCEEEEEHHHCCCCCCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
AMMDMGAIVCTRSKPKCEICPVNDLCQAKAQDRQLDFPTKKPKKEKPMKEAWFAIYYHDG
HHHHCCCEEEECCCCCEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHEEEEEEEECC
EVWLEQRPQTGIWGGLFCFPEQPENTLVDLSVRYGVEIKSTQQLIAFRHTFSHYHLDITP
EEEEECCCCCCCCCCEEECCCCCCCCEEEEEEEECEEEHHHHHHHHHHHHHHHEEEEHHH
VLITLVKKPNMVMEGTRGVWYNLSQPMTVGLAAPVQKLLDALPYEISNGE
HHHHHHCCCCEEEECCCCEEEECCCCCEEHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]