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The map label for this gene is cysH

Identifier: 209694005

GI number: 209694005

Start: 442904

End: 443665

Strand: Direct

Name: cysH

Synonym: VSAL_I0404

Alternate gene names: 209694005

Gene position: 442904-443665 (Clockwise)

Preceding gene: 209694004

Following gene: 209694008

Centisome position: 13.32

GC content: 40.03

Gene sequence:

>762_bases
ATGCCTAAATTGCAACTGTCAGAGCTGCTGTCATTAAATAAAGTCGAGCAAATTTTACGCTTAGCTGAAATTAATACCGA
GCTTGAACAACTGACGGCTCAAGAGCGAGTTTCTTGGGCTTTAGATAATCTAGAAGGTAAACCTGCAGTTTCTTCAAGCT
TTGGAATTCAGGCCGCGGTAATGTTGCAATTAGTCACGGACGTTAAGTCTGATACGCCTGTAATTTTAACGGACACTGGG
TATCTTTTTCCTGAAACGTACCAATTTATTGATACATTGACGGAGCGTCTGAACTTGAATTTACAAGTATTTACAGCAGA
TGAAACTCCCATGTGGCAAGAAGCACGCTACGGAAAGTTATGGGAGCAGGGCGTCGATGGACTTAAACATTATAACCGTT
TAAATAAAGTTCAGCCGATGCGAAGAGCATTAGATCAACTTCAAGTGGGTGTTTGGTTCTCGGGATTACGTCGTGATCAA
TCAAGTTCTCGTTCACATTTGCCTATTTTAAGTATTCAAAATGGGGTATTTAAATTTCTACCGGTTATCGATTGGAGTAA
TCAAGAGGTGCACTATTATTTGGAAGAGAAAGGATTACCTTATCATCCATTAAGAGACGAAGGATATTTGTCTGTTGGTG
ATACACATACAACTCAGAAATGGGAACCGGGAATGAAAGAAGAAGACACTCGATTTTTTGGATTAAAGAGAGAGTGTGGG
TTACATGAAGATGATGCTGAAGCGGAAGGCTCTGGAATTTAA

Upstream 100 bases:

>100_bases
GGTCGTTGGGCAACTGAGCGTCAAGAGAATGAAGGCTTTGGTGATTTTACCATTCGAGCCGGAATTGTGGATGAAGTGAA
AGTATCAAAAAGGGACTTTT

Downstream 100 bases:

>100_bases
TTTTTCTTTCTATCGTCGATAAAAATAAAAAGACAGCGATTATTCACTGTCTTTTTTTATTTCTCTTAAAAAGAAATTAT
TTCCATAATGAAGTCAGAGA

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MPKLQLSELLSLNKVEQILRLAEINTELEQLTAQERVSWALDNLEGKPAVSSSFGIQAAVMLQLVTDVKSDTPVILTDTG
YLFPETYQFIDTLTERLNLNLQVFTADETPMWQEARYGKLWEQGVDGLKHYNRLNKVQPMRRALDQLQVGVWFSGLRRDQ
SSSRSHLPILSIQNGVFKFLPVIDWSNQEVHYYLEEKGLPYHPLRDEGYLSVGDTHTTQKWEPGMKEEDTRFFGLKRECG
LHEDDAEAEGSGI

Sequences:

>Translated_253_residues
MPKLQLSELLSLNKVEQILRLAEINTELEQLTAQERVSWALDNLEGKPAVSSSFGIQAAVMLQLVTDVKSDTPVILTDTG
YLFPETYQFIDTLTERLNLNLQVFTADETPMWQEARYGKLWEQGVDGLKHYNRLNKVQPMRRALDQLQVGVWFSGLRRDQ
SSSRSHLPILSIQNGVFKFLPVIDWSNQEVHYYLEEKGLPYHPLRDEGYLSVGDTHTTQKWEPGMKEEDTRFFGLKRECG
LHEDDAEAEGSGI
>Mature_252_residues
PKLQLSELLSLNKVEQILRLAEINTELEQLTAQERVSWALDNLEGKPAVSSSFGIQAAVMLQLVTDVKSDTPVILTDTGY
LFPETYQFIDTLTERLNLNLQVFTADETPMWQEARYGKLWEQGVDGLKHYNRLNKVQPMRRALDQLQVGVWFSGLRRDQS
SSRSHLPILSIQNGVFKFLPVIDWSNQEVHYYLEEKGLPYHPLRDEGYLSVGDTHTTQKWEPGMKEEDTRFFGLKRECGL
HEDDAEAEGSGI

Specific function: Reduction of activated sulfate into sulfite

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily

Homologues:

Organism=Escherichia coli, GI1789121, Length=243, Percent_Identity=67.0781893004115, Blast_Score=348, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6325425, Length=227, Percent_Identity=32.15859030837, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSH_ALISL (B6ELM6)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002261933.1
- GeneID:   6988377
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0404
- HOGENOM:   HBG758022
- OMA:   TNFRPYE
- ProtClustDB:   PRK02090
- GO:   GO:0005737
- HAMAP:   MF_00063
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR011800
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00434
- TIGRFAMs:   TIGR02057

Pfam domain/function: PF01507 PAPS_reduct

EC number: =1.8.4.8

Molecular weight: Translated: 28999; Mature: 28868

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLQLSELLSLNKVEQILRLAEINTELEQLTAQERVSWALDNLEGKPAVSSSFGIQAAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
MLQLVTDVKSDTPVILTDTGYLFPETYQFIDTLTERLNLNLQVFTADETPMWQEARYGKL
HHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
WEQGVDGLKHYNRLNKVQPMRRALDQLQVGVWFSGLRRDQSSSRSHLPILSIQNGVFKFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEH
PVIDWSNQEVHYYLEEKGLPYHPLRDEGYLSVGDTHTTQKWEPGMKEEDTRFFGLKRECG
EEEECCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHCCCHHHCC
LHEDDAEAEGSGI
CCCCCCCCCCCCC
>Mature Secondary Structure 
PKLQLSELLSLNKVEQILRLAEINTELEQLTAQERVSWALDNLEGKPAVSSSFGIQAAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
MLQLVTDVKSDTPVILTDTGYLFPETYQFIDTLTERLNLNLQVFTADETPMWQEARYGKL
HHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
WEQGVDGLKHYNRLNKVQPMRRALDQLQVGVWFSGLRRDQSSSRSHLPILSIQNGVFKFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEH
PVIDWSNQEVHYYLEEKGLPYHPLRDEGYLSVGDTHTTQKWEPGMKEEDTRFFGLKRECG
EEEECCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHCCCHHHCC
LHEDDAEAEGSGI
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA