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The map label for this gene is cysI

Identifier: 209694004

GI number: 209694004

Start: 441181

End: 442911

Strand: Direct

Name: cysI

Synonym: VSAL_I0403

Alternate gene names: 209694004

Gene position: 441181-442911 (Clockwise)

Preceding gene: 209694003

Following gene: 209694005

Centisome position: 13.27

GC content: 41.54

Gene sequence:

>1731_bases
ATGAGCGAACAAATTGCAGTAAAAACAATCTTATCAGGCACAGAACTTGGCCCATTAGCGGATAACGAGCGTCTAAAGCG
TGAAAGCAAAAACCTTCGAGGTACGATCGTTGAGGATCTTCAAGATCGCATTACGGGCGGTTTTACCAACGATAATTTTC
AATTGATCCGTTTTCACGGCATGTATCAGCAAGATGACCGAGATATTCGAGCTGAGCGTGCAAAACAAAAATTAGAACCT
CTGCATAACGTAATGCTTCGTGCACGTATGCCGGGTGGAATTATTACCCCAAAACAGTGGTTAGCGATTGATAAGTTTGC
AGAAGAAAATACATCGTATGGCAGCTTACGTTTAACTACACGTCAAACCTTTCAGTTTCATGGTGTATTAAAGCCAAACA
TTAAATTAATGCATCAAACATTGAATAGCATTGGTATTGATTCGATTGCAACTGCGGGTGATGTAAACCGAAATGTATTG
TGTACGACCAATCCGGTGGAATCAGAGCTACATCAAGAAGCTTACGAGTGGGCAAAAAAAATCAGTGAGCATTTATTACC
AAAAACTCGTGCGTATGCTGAGATCTGGTTGGATGGTGAAAAATTAGAAACCACAGATGAAGAGCCTATTTTAGGCAGTA
ACTATCTTCCTCGTAAATTTAAAACTACCGTCGTTATTCCACCACAAAATGATGTTGATGTGCATGCCAATGATTTGAAT
TTCATTGCCATTGCAAATAACGGCAAATTAGTTGGTTTTAATGTATTGGTTGGTGGGGGCCTTGCGATGACGCATGGTGA
TACGGCAACGTACCCTCGTCGAGCGGATGATTTTGGTTTTGTGCCATTAGAAAAAACATTGGAAGTGGCGGCAGCGGTAG
TAACGACTCAGCGTGATTGGGGTAATCGTTCAAATCGTAAAAATGCAAAAACAAAATACACGTTAGATCGCGTGGGTGTT
GACGTCTTTAAAGCGGAAGTCGAAAAGCGTGCTGGTATTCAATTTGAAGCAAGCCGTCCTTATGAGCTAACAGAACGTGG
TGATCGAATTGGTTGGGTAGATGGCATTGATGGTAAGCACCATCTTGCATTATTTATCGAAAATGGTCGTTTATTAGACT
ACCCAGGTAAGCCATTAAAAACCGGTGTTGCTGAGATTGCTAAGATCCACCAAGGTGATTTTAGAATGACAGCCAATCAA
AACTTAATTGTTGCTGGGGTACCTGCTGAGCAAAAAGATCAGATAGAGCAAATTGCTCGAGAGCATGGTTTAATTGATGA
TGGGCATTCAGAACAACGTAAAAACTCAATGGCGTGTGTGGCTTTTCCTACTTGTCCATTAGCGATGGCAGAAGCAGAAC
GTTTCCTTCCTGAATTTGTCACAGAGATTGAAGGGGTACTGAAAAAACATAATTTGCCAGAAGACGATAATATTATTTTC
CGAGTGACGGGATGCCCTAATGGGTGCGGTCGAGCAATGCTTGCTGAGATTGGTTTAATCGGTAAAGCTCCGGGTCGTTA
TAATTTCCATTTAGGCGGTAATCGCAGTGGTACTCGTGTTCCTAAAATGTACAAAGAAAATATAACGGATCGCCAAATCT
TATCAGAGATTGATGAATTGGTTGGTCGTTGGGCAACTGAGCGTCAAGAGAATGAAGGCTTTGGTGATTTTACCATTCGA
GCCGGAATTGTGGATGAAGTGAAAGTATCAAAAAGGGACTTTTATGCCTAA

Upstream 100 bases:

>100_bases
TTACGATTGTTGAGCAGCAAGGAAAAAAGAACAGAGAAGAAGCAGAGCAATTTGTAAATGATCTGCGTAAGGCGAAACGT
TACCAAAGGGATGTGTACTA

Downstream 100 bases:

>100_bases
ATTGCAACTGTCAGAGCTGCTGTCATTAAATAAAGTCGAGCAAATTTTACGCTTAGCTGAAATTAATACCGAGCTTGAAC
AACTGACGGCTCAAGAGCGA

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MSEQIAVKTILSGTELGPLADNERLKRESKNLRGTIVEDLQDRITGGFTNDNFQLIRFHGMYQQDDRDIRAERAKQKLEP
LHNVMLRARMPGGIITPKQWLAIDKFAEENTSYGSLRLTTRQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVL
CTTNPVESELHQEAYEWAKKISEHLLPKTRAYAEIWLDGEKLETTDEEPILGSNYLPRKFKTTVVIPPQNDVDVHANDLN
FIAIANNGKLVGFNVLVGGGLAMTHGDTATYPRRADDFGFVPLEKTLEVAAAVVTTQRDWGNRSNRKNAKTKYTLDRVGV
DVFKAEVEKRAGIQFEASRPYELTERGDRIGWVDGIDGKHHLALFIENGRLLDYPGKPLKTGVAEIAKIHQGDFRMTANQ
NLIVAGVPAEQKDQIEQIAREHGLIDDGHSEQRKNSMACVAFPTCPLAMAEAERFLPEFVTEIEGVLKKHNLPEDDNIIF
RVTGCPNGCGRAMLAEIGLIGKAPGRYNFHLGGNRSGTRVPKMYKENITDRQILSEIDELVGRWATERQENEGFGDFTIR
AGIVDEVKVSKRDFYA

Sequences:

>Translated_576_residues
MSEQIAVKTILSGTELGPLADNERLKRESKNLRGTIVEDLQDRITGGFTNDNFQLIRFHGMYQQDDRDIRAERAKQKLEP
LHNVMLRARMPGGIITPKQWLAIDKFAEENTSYGSLRLTTRQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVL
CTTNPVESELHQEAYEWAKKISEHLLPKTRAYAEIWLDGEKLETTDEEPILGSNYLPRKFKTTVVIPPQNDVDVHANDLN
FIAIANNGKLVGFNVLVGGGLAMTHGDTATYPRRADDFGFVPLEKTLEVAAAVVTTQRDWGNRSNRKNAKTKYTLDRVGV
DVFKAEVEKRAGIQFEASRPYELTERGDRIGWVDGIDGKHHLALFIENGRLLDYPGKPLKTGVAEIAKIHQGDFRMTANQ
NLIVAGVPAEQKDQIEQIAREHGLIDDGHSEQRKNSMACVAFPTCPLAMAEAERFLPEFVTEIEGVLKKHNLPEDDNIIF
RVTGCPNGCGRAMLAEIGLIGKAPGRYNFHLGGNRSGTRVPKMYKENITDRQILSEIDELVGRWATERQENEGFGDFTIR
AGIVDEVKVSKRDFYA
>Mature_575_residues
SEQIAVKTILSGTELGPLADNERLKRESKNLRGTIVEDLQDRITGGFTNDNFQLIRFHGMYQQDDRDIRAERAKQKLEPL
HNVMLRARMPGGIITPKQWLAIDKFAEENTSYGSLRLTTRQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVLC
TTNPVESELHQEAYEWAKKISEHLLPKTRAYAEIWLDGEKLETTDEEPILGSNYLPRKFKTTVVIPPQNDVDVHANDLNF
IAIANNGKLVGFNVLVGGGLAMTHGDTATYPRRADDFGFVPLEKTLEVAAAVVTTQRDWGNRSNRKNAKTKYTLDRVGVD
VFKAEVEKRAGIQFEASRPYELTERGDRIGWVDGIDGKHHLALFIENGRLLDYPGKPLKTGVAEIAKIHQGDFRMTANQN
LIVAGVPAEQKDQIEQIAREHGLIDDGHSEQRKNSMACVAFPTCPLAMAEAERFLPEFVTEIEGVLKKHNLPEDDNIIFR
VTGCPNGCGRAMLAEIGLIGKAPGRYNFHLGGNRSGTRVPKMYKENITDRQILSEIDELVGRWATERQENEGFGDFTIRA
GIVDEVKVSKRDFYA

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=559, Percent_Identity=71.9141323792487, Blast_Score=865, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322597, Length=577, Percent_Identity=44.7140381282496, Blast_Score=509, Evalue=1e-145,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSI_ALISL (B6ELM5)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002261932.1
- GeneID:   6988378
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0403
- HOGENOM:   HBG626671
- OMA:   ESERVFP
- ProtClustDB:   PRK13504
- HAMAP:   MF_01540
- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397
- TIGRFAMs:   TIGR02041

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.1.2

Molecular weight: Translated: 64571; Mature: 64440

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQIAVKTILSGTELGPLADNERLKRESKNLRGTIVEDLQDRITGGFTNDNFQLIRFHG
CCCCEEEHHHHCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEC
MYQQDDRDIRAERAKQKLEPLHNVMLRARMPGGIITPKQWLAIDKFAEENTSYGSLRLTT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEE
RQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVLCTTNPVESELHQEAYEWAKK
EEEEEEECCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
ISEHLLPKTRAYAEIWLDGEKLETTDEEPILGSNYLPRKFKTTVVIPPQNDVDVHANDLN
HHHHHCCCHHCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCEE
FIAIANNGKLVGFNVLVGGGLAMTHGDTATYPRRADDFGFVPLEKTLEVAAAVVTTQRDW
EEEEECCCEEEEEEEEEECCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
GNRSNRKNAKTKYTLDRVGVDVFKAEVEKRAGIQFEASRPYELTERGDRIGWVDGIDGKH
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHCCCEEEEECCCCCCE
HLALFIENGRLLDYPGKPLKTGVAEIAKIHQGDFRMTANQNLIVAGVPAEQKDQIEQIAR
EEEEEEECCEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCHHHHHHHHHH
EHGLIDDGHSEQRKNSMACVAFPTCPLAMAEAERFLPEFVTEIEGVLKKHNLPEDDNIIF
HCCCCCCCCCHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
RVTGCPNGCGRAMLAEIGLIGKAPGRYNFHLGGNRSGTRVPKMYKENITDRQILSEIDEL
EEECCCCCHHHHHHHHHCCEECCCCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHH
VGRWATERQENEGFGDFTIRAGIVDEVKVSKRDFYA
HHHHHHHHHCCCCCCCEEEEECCHHHHHHCHHCCCC
>Mature Secondary Structure 
SEQIAVKTILSGTELGPLADNERLKRESKNLRGTIVEDLQDRITGGFTNDNFQLIRFHG
CCCEEEHHHHCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEC
MYQQDDRDIRAERAKQKLEPLHNVMLRARMPGGIITPKQWLAIDKFAEENTSYGSLRLTT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEE
RQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVLCTTNPVESELHQEAYEWAKK
EEEEEEECCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
ISEHLLPKTRAYAEIWLDGEKLETTDEEPILGSNYLPRKFKTTVVIPPQNDVDVHANDLN
HHHHHCCCHHCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCEE
FIAIANNGKLVGFNVLVGGGLAMTHGDTATYPRRADDFGFVPLEKTLEVAAAVVTTQRDW
EEEEECCCEEEEEEEEEECCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
GNRSNRKNAKTKYTLDRVGVDVFKAEVEKRAGIQFEASRPYELTERGDRIGWVDGIDGKH
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHCCCEEEEECCCCCCE
HLALFIENGRLLDYPGKPLKTGVAEIAKIHQGDFRMTANQNLIVAGVPAEQKDQIEQIAR
EEEEEEECCEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCHHHHHHHHHH
EHGLIDDGHSEQRKNSMACVAFPTCPLAMAEAERFLPEFVTEIEGVLKKHNLPEDDNIIF
HCCCCCCCCCHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
RVTGCPNGCGRAMLAEIGLIGKAPGRYNFHLGGNRSGTRVPKMYKENITDRQILSEIDEL
EEECCCCCHHHHHHHHHCCEECCCCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHH
VGRWATERQENEGFGDFTIRAGIVDEVKVSKRDFYA
HHHHHHHHHCCCCCCCEEEEECCHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA