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The map label for this gene is dnaB [H]

Identifier: 209693984

GI number: 209693984

Start: 421307

End: 422704

Strand: Direct

Name: dnaB [H]

Synonym: VSAL_I0382

Alternate gene names: 209693984

Gene position: 421307-422704 (Clockwise)

Preceding gene: 209693982

Following gene: 209693985

Centisome position: 12.67

GC content: 42.27

Gene sequence:

>1398_bases
ATGGCTGATAATCGAAATCAAAACCGCAAGTATGATGCACAAGTTGATGCGTTAAAAGTACCGCCGCATTCATTAGAGGC
AGAGCAATCTGTACTTGGTGGTTTACTACTTGATAATGAGCGGTGGGATACTGTCGCTGAAAAAGTGGTAGCTGTTGATT
TTTATAGCAGACCACACCGATTAATTTTTGAATCGGTTAAATCCATCCTAGAAGATAGTATGCCGTTGGATTTAATTACC
CTATCTGAACATCTTGAACGTCATGAAAAATTAGATTCAGTGGGCGGTTTTGCTTATCTTGCTGATTTAGTGAAAAACAC
CCCAAGTGCCGCGAATATAAATGCGTATGCTGATATTGTTCGTGAACGTGCGATGGTTCGTAACTTGATTAGTGTCGCCA
ATGAAATTGCTGACGCGGGTTATGATCCTCAAGGTCGAACTTCTGAAGATTTACTGGATATGGCCGAAAGTAAAGTTTTT
GCTATTGCCGAAGCGCGTACGACTGAGAATGAAGGGCCGCAAACGGTAGATACCATTCTTGAGCGAACATTAGAACGTAT
CGAGCTTTTATACCAAAGCCCACAAGATGGTGTCACCGGTGTCTCTACTGGATTCACGGATTTAAATAAGAAAACAGCGG
GTCTACAAGGGTCTGATTTAATTATTGTTGCGGCTCGTCCATCAATGGGTAAAACGACCTTTGCGATGAACTTATGTGAA
AACGCCGCAATGGACCAAGACAAGCCCGTTTTGATTTTCTCACTAGAGATGCCAGCGGAACAGCTAATGATGCGTATGTT
GGCTTCGATGTCTCGAGTTGATCAAACCAAAATTCGTACCGGTCAATTAGATGACGAAGATTGGGCGCGTATTTCGTCTA
CCATGGGTATTTTGACTCAGAAAAAGAATATGTATATTGATGATAGCTCAGGTTTAACGCCAACCGAGCTACGTTCTCGT
GCTCGTCGTGTTGCACGTGAGTCGGGAGGGTTAAGTATGATCATGATAGATTACCTTCAATTAATGCGTGTTCCTGGTAT
GCAAGATAATCGTACTCAAGAAATTTCAGAAATTTCACGCTCATTAAAAGCGCTAGCAAAAGAATTAAACGTTCCGGTTG
TTGCCCTGTCTCAGCTTAACCGTTCCCTAGAGCAACGTGCTGATAAACGCCCAATAAACTCGGATTTACGTGAATCTGGT
GCAATCGAGCAAGATGCCGATTTGATCATGTTTATTTACCGTGATGAAGTTTATAACCCAGAAAGTAACCGCAAAGGGAT
TGCTGAGATTATTCTTGGTAAACAGCGTAATGGTCCAATTGGCTCGGTAAGTTTAACCTTCCAAGGGCAATTTTCTCGCT
TTGATAATTACGCAGGTCCTGCGTTTGATGATGAATAA

Upstream 100 bases:

>100_bases
TATTTTGCGAAAGGTTATCAGGAGATACACGGATAGTTATTTCATCCCAATTTCTTTTGGTTATAATGTAGAGCTGAATT
TAGGATGGTGAAAAGTAGTA

Downstream 100 bases:

>100_bases
TAAGGCAAAAGTATGAGCTATATGACAGCCGCTGCTGCACAGATTGATATGTCTGCCTTAGCGCATAATTTGCAAAAAAT
TAAACAGCAAGCACCAAACA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MADNRNQNRKYDAQVDALKVPPHSLEAEQSVLGGLLLDNERWDTVAEKVVAVDFYSRPHRLIFESVKSILEDSMPLDLIT
LSEHLERHEKLDSVGGFAYLADLVKNTPSAANINAYADIVRERAMVRNLISVANEIADAGYDPQGRTSEDLLDMAESKVF
AIAEARTTENEGPQTVDTILERTLERIELLYQSPQDGVTGVSTGFTDLNKKTAGLQGSDLIIVAARPSMGKTTFAMNLCE
NAAMDQDKPVLIFSLEMPAEQLMMRMLASMSRVDQTKIRTGQLDDEDWARISSTMGILTQKKNMYIDDSSGLTPTELRSR
ARRVARESGGLSMIMIDYLQLMRVPGMQDNRTQEISEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPINSDLRESG
AIEQDADLIMFIYRDEVYNPESNRKGIAEIILGKQRNGPIGSVSLTFQGQFSRFDNYAGPAFDDE

Sequences:

>Translated_465_residues
MADNRNQNRKYDAQVDALKVPPHSLEAEQSVLGGLLLDNERWDTVAEKVVAVDFYSRPHRLIFESVKSILEDSMPLDLIT
LSEHLERHEKLDSVGGFAYLADLVKNTPSAANINAYADIVRERAMVRNLISVANEIADAGYDPQGRTSEDLLDMAESKVF
AIAEARTTENEGPQTVDTILERTLERIELLYQSPQDGVTGVSTGFTDLNKKTAGLQGSDLIIVAARPSMGKTTFAMNLCE
NAAMDQDKPVLIFSLEMPAEQLMMRMLASMSRVDQTKIRTGQLDDEDWARISSTMGILTQKKNMYIDDSSGLTPTELRSR
ARRVARESGGLSMIMIDYLQLMRVPGMQDNRTQEISEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPINSDLRESG
AIEQDADLIMFIYRDEVYNPESNRKGIAEIILGKQRNGPIGSVSLTFQGQFSRFDNYAGPAFDDE
>Mature_464_residues
ADNRNQNRKYDAQVDALKVPPHSLEAEQSVLGGLLLDNERWDTVAEKVVAVDFYSRPHRLIFESVKSILEDSMPLDLITL
SEHLERHEKLDSVGGFAYLADLVKNTPSAANINAYADIVRERAMVRNLISVANEIADAGYDPQGRTSEDLLDMAESKVFA
IAEARTTENEGPQTVDTILERTLERIELLYQSPQDGVTGVSTGFTDLNKKTAGLQGSDLIIVAARPSMGKTTFAMNLCEN
AAMDQDKPVLIFSLEMPAEQLMMRMLASMSRVDQTKIRTGQLDDEDWARISSTMGILTQKKNMYIDDSSGLTPTELRSRA
RRVARESGGLSMIMIDYLQLMRVPGMQDNRTQEISEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPINSDLRESGA
IEQDADLIMFIYRDEVYNPESNRKGIAEIILGKQRNGPIGSVSLTFQGQFSRFDNYAGPAFDDE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=457, Percent_Identity=78.5557986870897, Blast_Score=745, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51847; Mature: 51716

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNRNQNRKYDAQVDALKVPPHSLEAEQSVLGGLLLDNERWDTVAEKVVAVDFYSRPHR
CCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHCCCHH
LIFESVKSILEDSMPLDLITLSEHLERHEKLDSVGGFAYLADLVKNTPSAANINAYADIV
HHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHH
RERAMVRNLISVANEIADAGYDPQGRTSEDLLDMAESKVFAIAEARTTENEGPQTVDTIL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEEEEHHCCCCCCCHHHHHHH
ERTLERIELLYQSPQDGVTGVSTGFTDLNKKTAGLQGSDLIIVAARPSMGKTTFAMNLCE
HHHHHHHHHHHHCCCCCCCCCCCCCHHCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHH
NAAMDQDKPVLIFSLEMPAEQLMMRMLASMSRVDQTKIRTGQLDDEDWARISSTMGILTQ
HCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEE
KKNMYIDDSSGLTPTELRSRARRVARESGGLSMIMIDYLQLMRVPGMQDNRTQEISEISR
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHH
SLKALAKELNVPVVALSQLNRSLEQRADKRPINSDLRESGAIEQDADLIMFIYRDEVYNP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCC
ESNRKGIAEIILGKQRNGPIGSVSLTFQGQFSRFDNYAGPAFDDE
CCCCCHHHHHHHCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ADNRNQNRKYDAQVDALKVPPHSLEAEQSVLGGLLLDNERWDTVAEKVVAVDFYSRPHR
CCCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHCCCHH
LIFESVKSILEDSMPLDLITLSEHLERHEKLDSVGGFAYLADLVKNTPSAANINAYADIV
HHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHH
RERAMVRNLISVANEIADAGYDPQGRTSEDLLDMAESKVFAIAEARTTENEGPQTVDTIL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEEEEHHCCCCCCCHHHHHHH
ERTLERIELLYQSPQDGVTGVSTGFTDLNKKTAGLQGSDLIIVAARPSMGKTTFAMNLCE
HHHHHHHHHHHHCCCCCCCCCCCCCHHCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHH
NAAMDQDKPVLIFSLEMPAEQLMMRMLASMSRVDQTKIRTGQLDDEDWARISSTMGILTQ
HCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEE
KKNMYIDDSSGLTPTELRSRARRVARESGGLSMIMIDYLQLMRVPGMQDNRTQEISEISR
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHH
SLKALAKELNVPVVALSQLNRSLEQRADKRPINSDLRESGAIEQDADLIMFIYRDEVYNP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCC
ESNRKGIAEIILGKQRNGPIGSVSLTFQGQFSRFDNYAGPAFDDE
CCCCCHHHHHHHCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]