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The map label for this gene is leuD

Identifier: 209693977

GI number: 209693977

Start: 409804

End: 410406

Strand: Reverse

Name: leuD

Synonym: VSAL_I0374

Alternate gene names: 209693977

Gene position: 410406-409804 (Counterclockwise)

Preceding gene: 209693978

Following gene: 209693976

Centisome position: 12.34

GC content: 40.46

Gene sequence:

>603_bases
ATGTCAGGTTTTAAACAACATACAGGTCTAGTCGTTCCTTTAGATACGGCAAACATTGATACCGATGCCATCATTCCAAA
ACAGTTTTTACAAAAAGTAAATCGCACCGGTTTTGGTAAACACTTATTTCATGACTGGCGCTTTCTTGATGATGCCGGTC
AACAGCCAAATCCAGAATTTGTAATGAATGCCCCTCGTTATCAAGGCGCAAGTATTCTACTTGCCCGTGAAAACTTTGGT
TGTGGCTCATCGCGTGAACACGCACCTTGGGCTTTGGCGGATTACGGCATCCAAGTGATGATAGCACCAAGCTTTGCTGA
TATTTTTTACGGCAATTCAATCAATAACCAAATGATCCCTGTTCGTTTAACTGATATTGAAGTGGATGAAATATTCCAAT
TCGTTGAAGCGAATGAAGGCGCTGAAGTTGCGGTTGATTTGGAAGCTATGTTAGTTAGCGCTAACGGTAAGCAGTATTCT
TTTGAGATTGATGAATTTCGTCGCCACTGTTTATTGAATGGTTTAGACAATATAGGCTTGACGCTACAGCACGCTGATAA
AATTTCAGAATATGAAGCGAAGATTCCGAATTTCTTGAAATAG

Upstream 100 bases:

>100_bases
GAACTCACCTAGTAAGCCCAGCCATGGCAGCGGCAGCCGCGATTGCGGGTCATTTTGTTGATATTCGTACCATCACAGAA
AAAGCATAAAGGAATTTACT

Downstream 100 bases:

>100_bases
GATTCAGGAAATAATAGAGGTTGATATGAACGTATCAACCTCTTTTTTGTCTATAGAATAGAGTCATCTATTATAAATAA
TACCAACCTAAATAAATCGT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSGFKQHTGLVVPLDTANIDTDAIIPKQFLQKVNRTGFGKHLFHDWRFLDDAGQQPNPEFVMNAPRYQGASILLARENFG
CGSSREHAPWALADYGIQVMIAPSFADIFYGNSINNQMIPVRLTDIEVDEIFQFVEANEGAEVAVDLEAMLVSANGKQYS
FEIDEFRRHCLLNGLDNIGLTLQHADKISEYEAKIPNFLK

Sequences:

>Translated_200_residues
MSGFKQHTGLVVPLDTANIDTDAIIPKQFLQKVNRTGFGKHLFHDWRFLDDAGQQPNPEFVMNAPRYQGASILLARENFG
CGSSREHAPWALADYGIQVMIAPSFADIFYGNSINNQMIPVRLTDIEVDEIFQFVEANEGAEVAVDLEAMLVSANGKQYS
FEIDEFRRHCLLNGLDNIGLTLQHADKISEYEAKIPNFLK
>Mature_199_residues
SGFKQHTGLVVPLDTANIDTDAIIPKQFLQKVNRTGFGKHLFHDWRFLDDAGQQPNPEFVMNAPRYQGASILLARENFGC
GSSREHAPWALADYGIQVMIAPSFADIFYGNSINNQMIPVRLTDIEVDEIFQFVEANEGAEVAVDLEAMLVSANGKQYSF
EIDEFRRHCLLNGLDNIGLTLQHADKISEYEAKIPNFLK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=196, Percent_Identity=73.9795918367347, Blast_Score=313, Evalue=4e-87,
Organism=Saccharomyces cerevisiae, GI6321429, Length=199, Percent_Identity=50.251256281407, Blast_Score=195, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_ALISL (B6ELJ7)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002261905.1
- GeneID:   6986449
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0374
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22464; Mature: 22332

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFKQHTGLVVPLDTANIDTDAIIPKQFLQKVNRTGFGKHLFHDWRFLDDAGQQPNPEF
CCCCCHHCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCE
VMNAPRYQGASILLARENFGCGSSREHAPWALADYGIQVMIAPSFADIFYGNSINNQMIP
EEECCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEEEEECCHHHHHCCCCCCCCEEE
VRLTDIEVDEIFQFVEANEGAEVAVDLEAMLVSANGKQYSFEIDEFRRHCLLNGLDNIGL
EEEECCCHHHHHHHHHCCCCCEEEEEEEEEEEECCCCEEEEEHHHHHHHHHHCCCCCCCE
TLQHADKISEYEAKIPNFLK
EEEHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SGFKQHTGLVVPLDTANIDTDAIIPKQFLQKVNRTGFGKHLFHDWRFLDDAGQQPNPEF
CCCCHHCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCE
VMNAPRYQGASILLARENFGCGSSREHAPWALADYGIQVMIAPSFADIFYGNSINNQMIP
EEECCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEEEEECCHHHHHCCCCCCCCEEE
VRLTDIEVDEIFQFVEANEGAEVAVDLEAMLVSANGKQYSFEIDEFRRHCLLNGLDNIGL
EEEECCCHHHHHHHHHCCCCCEEEEEEEEEEEECCCCEEEEEHHHHHHHHHHCCCCCCCE
TLQHADKISEYEAKIPNFLK
EEEHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA