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The map label for this gene is obgE

Identifier: 209693966

GI number: 209693966

Start: 397929

End: 399107

Strand: Direct

Name: obgE

Synonym: VSAL_I0363

Alternate gene names: 209693966

Gene position: 397929-399107 (Clockwise)

Preceding gene: 209693965

Following gene: 209693967

Centisome position: 11.97

GC content: 43.94

Gene sequence:

>1179_bases
ATGAAATTCGTTGATGAAGCGACAATTAAAGTAGATGCAGGCGACGGCGGTAACGGTACTGTTAGTTTTTGGCGTGAAAA
ATTCGTAGCTAAAGGCGGCCCTGATGGCGGCGATGGCGGCGATGGTGGTGATGTTTACCTAGAAGCTGATGAAAACCTAA
ATACCCTAATCGATTACCGTTTTAACCGTTTCTATGATGCTGAGCGTGGTAAAAATGGTGGTGGTACGAATAGTACTGGC
CGACGTGGTGAAGATATTACATTGAAAGTACCTGTTGGTACGCGTGCTATCGATATTGATACTGGCGAGAAAGTTGCAGA
GCTAATGGCTCACGGTATGAAGATCATGGTAGCTAAAGGCGGCTGGCATGGTTTAGGTAATACTCGTTTTAAATCATCAG
TTAACCGTGCTCCTCGTCAAAAGACAATGGGAACAAAGGGTGAAGTTCGTGAACTTCGTCTAGAGCTTCTTCTTCTTGCT
GATGTTGGTATGCTTGGCTTGCCAAATGCGGGTAAATCAACGTTTATTCGTGCAGTATCTGCAGCAAAACCAAAAGTAGC
TGATTATCCGTTTACGACGCTTATCCCTAGTTTAGGTGTAGTACGTGCTCGTGGTAATAAGAGCTTCGTTATTGCGGATA
TTCCTGGTCTGATTGAAGGTGCTGCTGAAGGTGCCGGTCTTGGTGTTCGTTTCTTGAAGCACCTTGAGCGTTGTCGTGTA
TTGCTTCACGTGATCGATATTCTTCCTATTGATGGCAGTGATCCTGTTCAAAATGCCCTAACGATCATTGATGAGTTAGA
GCGATACAGTGAGAAAGTAGCTGGAAAACCTCGTTGGTTATTGTTTAACAAAACGGATCTCCTACTTGAAGCTGAAGCCG
ATGAGAAGATCGCAGAGATCCTTGAAGCATTAGCTTGGGAAGGCGCTCACTTCAAAATTGCTGCGGTAAGCCGTACAGGT
ACTCAAGAAGTGTGTAATGAACTTTCTGACTTTATGGATACGTTACCTAAAGAAATCATGACAGATGAAGAAAAAGCGGC
GCTTAAAGTCGACTTCATGTGGGATGATTATCATAAAGACGCAATGTCTGGTAAAAATGTGGTTACTGAAGACGGTGACG
ACGATGATGATTGGGATGACGAAGAAGATGATGGTCACGTTATCTATGCTCGTGATTAA

Upstream 100 bases:

>100_bases
ATTGACGATCCACTCTCACCCTATCATTTTGGTCTACAGTTAATAAGCCAACCGTACCCAATAATGACAAGAGTATTTAC
GCTACAGCTCGGAGAAGAAA

Downstream 100 bases:

>100_bases
GTCTTAATCAAGACAATTATTATAAAAAACCGCACTTGTTGCGGTTTTTTTTATTTAAGTCATTAAAAGTAAGAAGAGAA
AAGATTAGAATCATTTTATA

Product: GTPase ObgE

Products: NA

Alternate protein names: GTP-binding protein obg

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKFVDEATIKVDAGDGGNGTVSFWREKFVAKGGPDGGDGGDGGDVYLEADENLNTLIDYRFNRFYDAERGKNGGGTNSTG
RRGEDITLKVPVGTRAIDIDTGEKVAELMAHGMKIMVAKGGWHGLGNTRFKSSVNRAPRQKTMGTKGEVRELRLELLLLA
DVGMLGLPNAGKSTFIRAVSAAKPKVADYPFTTLIPSLGVVRARGNKSFVIADIPGLIEGAAEGAGLGVRFLKHLERCRV
LLHVIDILPIDGSDPVQNALTIIDELERYSEKVAGKPRWLLFNKTDLLLEAEADEKIAEILEALAWEGAHFKIAAVSRTG
TQEVCNELSDFMDTLPKEIMTDEEKAALKVDFMWDDYHKDAMSGKNVVTEDGDDDDDWDDEEDDGHVIYARD

Sequences:

>Translated_392_residues
MKFVDEATIKVDAGDGGNGTVSFWREKFVAKGGPDGGDGGDGGDVYLEADENLNTLIDYRFNRFYDAERGKNGGGTNSTG
RRGEDITLKVPVGTRAIDIDTGEKVAELMAHGMKIMVAKGGWHGLGNTRFKSSVNRAPRQKTMGTKGEVRELRLELLLLA
DVGMLGLPNAGKSTFIRAVSAAKPKVADYPFTTLIPSLGVVRARGNKSFVIADIPGLIEGAAEGAGLGVRFLKHLERCRV
LLHVIDILPIDGSDPVQNALTIIDELERYSEKVAGKPRWLLFNKTDLLLEAEADEKIAEILEALAWEGAHFKIAAVSRTG
TQEVCNELSDFMDTLPKEIMTDEEKAALKVDFMWDDYHKDAMSGKNVVTEDGDDDDDWDDEEDDGHVIYARD
>Mature_392_residues
MKFVDEATIKVDAGDGGNGTVSFWREKFVAKGGPDGGDGGDGGDVYLEADENLNTLIDYRFNRFYDAERGKNGGGTNSTG
RRGEDITLKVPVGTRAIDIDTGEKVAELMAHGMKIMVAKGGWHGLGNTRFKSSVNRAPRQKTMGTKGEVRELRLELLLLA
DVGMLGLPNAGKSTFIRAVSAAKPKVADYPFTTLIPSLGVVRARGNKSFVIADIPGLIEGAAEGAGLGVRFLKHLERCRV
LLHVIDILPIDGSDPVQNALTIIDELERYSEKVAGKPRWLLFNKTDLLLEAEADEKIAEILEALAWEGAHFKIAAVSRTG
TQEVCNELSDFMDTLPKEIMTDEEKAALKVDFMWDDYHKDAMSGKNVVTEDGDDDDDWDDEEDDGHVIYARD

Specific function: An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in t

COG id: COG0536

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI24308117, Length=305, Percent_Identity=41.3114754098361, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI111955139, Length=251, Percent_Identity=39.4422310756972, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI111955063, Length=144, Percent_Identity=43.75, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI4758796, Length=127, Percent_Identity=35.4330708661417, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI58761500, Length=137, Percent_Identity=31.3868613138686, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789574, Length=362, Percent_Identity=68.5082872928177, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1787454, Length=129, Percent_Identity=29.4573643410853, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17508313, Length=331, Percent_Identity=36.2537764350453, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17552324, Length=324, Percent_Identity=29.9382716049383, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17509631, Length=128, Percent_Identity=33.59375, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17555344, Length=118, Percent_Identity=33.8983050847458, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321962, Length=178, Percent_Identity=37.6404494382022, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319281, Length=128, Percent_Identity=31.25, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24585318, Length=292, Percent_Identity=36.986301369863, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI20129375, Length=288, Percent_Identity=37.8472222222222, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24640873, Length=128, Percent_Identity=32.03125, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24640877, Length=128, Percent_Identity=32.03125, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24640875, Length=128, Percent_Identity=32.03125, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17981711, Length=127, Percent_Identity=31.496062992126, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OBG_ALISL (B6EL43)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002261894.1
- GeneID:   6989169
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0363
- HOGENOM:   HBG716038
- OMA:   LIDYRFN
- ProtClustDB:   PRK12298
- GO:   GO:0005737
- HAMAP:   MF_01454
- InterPro:   IPR014100
- InterPro:   IPR006074
- InterPro:   IPR006073
- InterPro:   IPR006169
- InterPro:   IPR002917
- InterPro:   IPR005225
- Gene3D:   G3DSA:2.70.210.12
- PANTHER:   PTHR11702:SF3
- PIRSF:   PIRSF002401
- PRINTS:   PR00326
- TIGRFAMs:   TIGR02729
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01018 GTP1_OBG; PF01926 MMR_HSR1; SSF82051 GTP1_OBG_sub

EC number: NA

Molecular weight: Translated: 42833; Mature: 42833

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00905 GTP1_OBG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVDEATIKVDAGDGGNGTVSFWREKFVAKGGPDGGDGGDGGDVYLEADENLNTLIDYR
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHH
FNRFYDAERGKNGGGTNSTGRRGEDITLKVPVGTRAIDIDTGEKVAELMAHGMKIMVAKG
HHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCHHHHHHHHHCCEEEEEECC
GWHGLGNTRFKSSVNRAPRQKTMGTKGEVRELRLELLLLADVGMLGLPNAGKSTFIRAVS
CCCCCCCCHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AAKPKVADYPFTTLIPSLGVVRARGNKSFVIADIPGLIEGAAEGAGLGVRFLKHLERCRV
HCCCCCCCCCHHHHHCCCCEEEECCCCEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHH
LLHVIDILPIDGSDPVQNALTIIDELERYSEKVAGKPRWLLFNKTDLLLEAEADEKIAEI
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCHHHHHHH
LEALAWEGAHFKIAAVSRTGTQEVCNELSDFMDTLPKEIMTDEEKAALKVDFMWDDYHKD
HHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECHHHHH
AMSGKNVVTEDGDDDDDWDDEEDDGHVIYARD
HCCCCCEECCCCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MKFVDEATIKVDAGDGGNGTVSFWREKFVAKGGPDGGDGGDGGDVYLEADENLNTLIDYR
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHH
FNRFYDAERGKNGGGTNSTGRRGEDITLKVPVGTRAIDIDTGEKVAELMAHGMKIMVAKG
HHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCHHHHHHHHHCCEEEEEECC
GWHGLGNTRFKSSVNRAPRQKTMGTKGEVRELRLELLLLADVGMLGLPNAGKSTFIRAVS
CCCCCCCCHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AAKPKVADYPFTTLIPSLGVVRARGNKSFVIADIPGLIEGAAEGAGLGVRFLKHLERCRV
HCCCCCCCCCHHHHHCCCCEEEECCCCEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHH
LLHVIDILPIDGSDPVQNALTIIDELERYSEKVAGKPRWLLFNKTDLLLEAEADEKIAEI
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCHHHHHHH
LEALAWEGAHFKIAAVSRTGTQEVCNELSDFMDTLPKEIMTDEEKAALKVDFMWDDYHKD
HHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECHHHHH
AMSGKNVVTEDGDDDDDWDDEEDDGHVIYARD
HCCCCCEECCCCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA