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The map label for this gene is rmlB [H]

Identifier: 209693795

GI number: 209693795

Start: 220081

End: 221172

Strand: Reverse

Name: rmlB [H]

Synonym: VSAL_I0180

Alternate gene names: 209693795

Gene position: 221172-220081 (Counterclockwise)

Preceding gene: 209693796

Following gene: 209693794

Centisome position: 6.65

GC content: 39.93

Gene sequence:

>1092_bases
ATGAATATTTTAGTCACCGGCGGCGCAGGTTTTATTGGCTCAGCAGTTATTCGCCACATTATCAGCAATACATCAAACTC
TGTCATCAATGTTGATTCTCTTACCTACGCAGGAAATCTAGAGTCTTTGAGTCAGGTTGAAGCATCAGATCGTTATTCAT
TTGAACACGTTGATATCTGTAATCGCACTGAACTTGACCGTATTTTTTCTGAATATCAACCTGATGCAGTCATGCACCTA
GCTGCTGAGTCACATGTAGATCGCTCAATAGATGGCCCTGCTGCGTTCATTGAAACTAATATTGTCGGTACTTATACTTT
ACTTGAAGCAACGCGTGCCTATTGGAATTTGTTAGCTGATGATAAAAAGCAAGCCTTTCGTTTTCACCATATATCAACGG
ATGAAGTGTATGGTGATTTAGAAGGTACAGATGATTTGTTTACCGAAACAACCCCTTATGAGCCATCAAGCCCTTATTCC
GCGTCTAAAGCTTCTTCAGATCATTTAGTTCGTGCATGGCAACGTACTTATGGCCTACCAACAGTTATTACTAATTGTTC
AAATAACTATGGGCCATATCATTTCCCTGAAAAGCTGATCCCATTAATGATTTTAAATGCCTTAGAAGGTAAGTATTTAC
CTGTTTATGGCAATGGAATGCAAATTCGAGACTGGTTGTTTGTAGAAGACCATGCTCGCGCGCTATATAAAGTCGTTACT
GAAGGTGAGATTGGCGAAACCTACAATATTGGTGGTCATAACGAAAAAGCCAATATTGAAGTAGTTAAGACCATTTGCTC
ATTACTTGAAGAGTTAGTGCCGAACAAACCTGAAGGTGTTGCTCAGTACCTTGATTTAATTACCTACGTAACAGACCGCC
CTGGTCATGATGTTCGTTATGCTATTGATGCTTCAAAAATTGAGCATGAATTAGGGTGGAAACCAGAAGAGAGCTTTGAA
TCAGGGATCCGAAAAACCGTTCAATGGTATTTAGACAATAAACAATGGTGGTCACGCGTCCTTGATGGGTCTTATGCAGG
AGAACGTTTAGGCGTATCAAGTGAACAGGTTGTACATAAGGAAATTAGATAA

Upstream 100 bases:

>100_bases
CCGTCCAATGAAAGATGCTGAATTAAATTATCAACATCTTCAAGAAGCATTAGAGCAGATTAGTCGATTTAGTAAGTAGA
TTTTAGATTAAAGGATAAAT

Downstream 100 bases:

>100_bases
TGAAAGGGATTGTTTTAGCCGGAGGCTCAGGCACACGCCTATATCCATTAACTCGTGGAGTATCAAAGCAGTTATTACCT
ATTTACGACAAACCAATGGT

Product: dTDP-D-glucose-4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MNILVTGGAGFIGSAVIRHIISNTSNSVINVDSLTYAGNLESLSQVEASDRYSFEHVDICNRTELDRIFSEYQPDAVMHL
AAESHVDRSIDGPAAFIETNIVGTYTLLEATRAYWNLLADDKKQAFRFHHISTDEVYGDLEGTDDLFTETTPYEPSSPYS
ASKASSDHLVRAWQRTYGLPTVITNCSNNYGPYHFPEKLIPLMILNALEGKYLPVYGNGMQIRDWLFVEDHARALYKVVT
EGEIGETYNIGGHNEKANIEVVKTICSLLEELVPNKPEGVAQYLDLITYVTDRPGHDVRYAIDASKIEHELGWKPEESFE
SGIRKTVQWYLDNKQWWSRVLDGSYAGERLGVSSEQVVHKEIR

Sequences:

>Translated_363_residues
MNILVTGGAGFIGSAVIRHIISNTSNSVINVDSLTYAGNLESLSQVEASDRYSFEHVDICNRTELDRIFSEYQPDAVMHL
AAESHVDRSIDGPAAFIETNIVGTYTLLEATRAYWNLLADDKKQAFRFHHISTDEVYGDLEGTDDLFTETTPYEPSSPYS
ASKASSDHLVRAWQRTYGLPTVITNCSNNYGPYHFPEKLIPLMILNALEGKYLPVYGNGMQIRDWLFVEDHARALYKVVT
EGEIGETYNIGGHNEKANIEVVKTICSLLEELVPNKPEGVAQYLDLITYVTDRPGHDVRYAIDASKIEHELGWKPEESFE
SGIRKTVQWYLDNKQWWSRVLDGSYAGERLGVSSEQVVHKEIR
>Mature_363_residues
MNILVTGGAGFIGSAVIRHIISNTSNSVINVDSLTYAGNLESLSQVEASDRYSFEHVDICNRTELDRIFSEYQPDAVMHL
AAESHVDRSIDGPAAFIETNIVGTYTLLEATRAYWNLLADDKKQAFRFHHISTDEVYGDLEGTDDLFTETTPYEPSSPYS
ASKASSDHLVRAWQRTYGLPTVITNCSNNYGPYHFPEKLIPLMILNALEGKYLPVYGNGMQIRDWLFVEDHARALYKVVT
EGEIGETYNIGGHNEKANIEVVKTICSLLEELVPNKPEGVAQYLDLITYVTDRPGHDVRYAIDASKIEHELGWKPEESFE
SGIRKTVQWYLDNKQWWSRVLDGSYAGERLGVSSEQVVHKEIR

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=339, Percent_Identity=35.3982300884956, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI42516563, Length=333, Percent_Identity=24.3243243243243, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4504031, Length=346, Percent_Identity=25.7225433526012, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI56237023, Length=362, Percent_Identity=25.6906077348066, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI56118217, Length=362, Percent_Identity=25.6906077348066, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI189083684, Length=362, Percent_Identity=25.6906077348066, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=78.6324786324786, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1788353, Length=353, Percent_Identity=73.0878186968839, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1788366, Length=301, Percent_Identity=27.2425249169435, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786974, Length=355, Percent_Identity=26.1971830985915, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=338, Percent_Identity=36.6863905325444, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=33.5347432024169, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71982035, Length=373, Percent_Identity=26.5415549597855, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=374, Percent_Identity=26.2032085561497, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=339, Percent_Identity=24.7787610619469, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI133901790, Length=192, Percent_Identity=27.0833333333333, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=192, Percent_Identity=27.0833333333333, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=192, Percent_Identity=27.0833333333333, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=192, Percent_Identity=27.0833333333333, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=192, Percent_Identity=27.0833333333333, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=198, Percent_Identity=25.7575757575758, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=365, Percent_Identity=26.3013698630137, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19923002, Length=371, Percent_Identity=27.4932614555256, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21356223, Length=333, Percent_Identity=24.9249249249249, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24158427, Length=284, Percent_Identity=24.6478873239437, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40920; Mature: 40920

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVTGGAGFIGSAVIRHIISNTSNSVINVDSLTYAGNLESLSQVEASDRYSFEHVDIC
CEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCEEECCCHHHHHHHHHCCCCCCCCCCCC
NRTELDRIFSEYQPDAVMHLAAESHVDRSIDGPAAFIETNIVGTYTLLEATRAYWNLLAD
CHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHEEECCCCHHHHHHHHHHHHHHHHHC
DKKQAFRFHHISTDEVYGDLEGTDDLFTETTPYEPSSPYSASKASSDHLVRAWQRTYGLP
CHHHHHHEECCCHHHHCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
TVITNCSNNYGPYHFPEKLIPLMILNALEGKYLPVYGNGMQIRDWLFVEDHARALYKVVT
HHEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEHHHHHHHHHHHC
EGEIGETYNIGGHNEKANIEVVKTICSLLEELVPNKPEGVAQYLDLITYVTDRPGHDVRY
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEE
AIDASKIEHELGWKPEESFESGIRKTVQWYLDNKQWWSRVLDGSYAGERLGVSSEQVVHK
EECHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHH
EIR
HCC
>Mature Secondary Structure
MNILVTGGAGFIGSAVIRHIISNTSNSVINVDSLTYAGNLESLSQVEASDRYSFEHVDIC
CEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCEEECCCHHHHHHHHHCCCCCCCCCCCC
NRTELDRIFSEYQPDAVMHLAAESHVDRSIDGPAAFIETNIVGTYTLLEATRAYWNLLAD
CHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHEEECCCCHHHHHHHHHHHHHHHHHC
DKKQAFRFHHISTDEVYGDLEGTDDLFTETTPYEPSSPYSASKASSDHLVRAWQRTYGLP
CHHHHHHEECCCHHHHCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
TVITNCSNNYGPYHFPEKLIPLMILNALEGKYLPVYGNGMQIRDWLFVEDHARALYKVVT
HHEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEHHHHHHHHHHHC
EGEIGETYNIGGHNEKANIEVVKTICSLLEELVPNKPEGVAQYLDLITYVTDRPGHDVRY
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEE
AIDASKIEHELGWKPEESFESGIRKTVQWYLDNKQWWSRVLDGSYAGERLGVSSEQVVHK
EECHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHH
EIR
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]