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The map label for this gene is rmlA [H]

Identifier: 209693794

GI number: 209693794

Start: 219197

End: 220081

Strand: Reverse

Name: rmlA [H]

Synonym: VSAL_I0179

Alternate gene names: 209693794

Gene position: 220081-219197 (Counterclockwise)

Preceding gene: 209693795

Following gene: 209693793

Centisome position: 6.62

GC content: 38.42

Gene sequence:

>885_bases
ATGAAAGGGATTGTTTTAGCCGGAGGCTCAGGCACACGCCTATATCCATTAACTCGTGGAGTATCAAAGCAGTTATTACC
TATTTACGACAAACCAATGGTGTTTTATCCGATATCGACATTAATGCTCGCCGGTATTAAAGATATTTTAATTATCACTA
CTCCAGAAGATAACGCAGGTTTTAAACGCTTATTAGGCGATGGCTCTGATTTTGGGATTAACCTTGAATACGCAATTCAA
CCAAGCCCTGATGGTTTAGCTCAAGCTTTTATTATTGGCGAAGAGTTCATTGGTGATGACCCAGTCTGCTTAGTACTTGG
TGATAATATTTTCTATGGTCAATCTTTCTCTAAAACGTTAAAGAATGCGGCTTCACGTGACTTAGGTGCCACCGTATTTG
GTTATCAAGTAAAAGACCCAGAGCGTTTTGGTGTGGTTGAGTTTGATGAAAAAATGAAAGCAATCTCAATTGAAGAAAAG
CCACTAAAACCTAAATCGAATTATGCGGTGACAGGATTATACTTTTACGATAACCGCGTAGTTGAAATGGCAAAACAAGT
TAAGCCTTCTCATCGTGGTGAGCTTGAAATTACCACGCTTAATGAAATGTATCTTAACGATGGTTCTCTTAATGTTGAAT
TATTAGCTCGTGGCTTTGCGTGGCTAGATACAGGGACTCATGAGAGTCTGCATGAAGCCTCTTCATTTGTGCAAACCATT
GAGCATATACAAGGCTTAAAAGTGGCGTGTTTAGAAGAAATATCATGGCGTAATGGTTGGTTATCAGATGAGCAAATATT
AGATCTCGCAACCCCGATGATGAAGAATGAGTATGGCCAATATTTAACCCGATTAATTAACGATAATAAACAGAAAGAAT
TATAA

Upstream 100 bases:

>100_bases
TAGACAATAAACAATGGTGGTCACGCGTCCTTGATGGGTCTTATGCAGGAGAACGTTTAGGCGTATCAAGTGAACAGGTT
GTACATAAGGAAATTAGATA

Downstream 100 bases:

>100_bases
TGCTAAAGGTCCTTATTACAGGTGCAACCGGGCAAGTCGGTAGTCATTTAACTCTCTTATTAACGGGAAGAGCTCAACTG
CTCGCGGTCGATAGGTCTGA

Product: glucose-1-phosphate thymidylyltransferase RmlA

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKGIVLAGGSGTRLYPLTRGVSKQLLPIYDKPMVFYPISTLMLAGIKDILIITTPEDNAGFKRLLGDGSDFGINLEYAIQ
PSPDGLAQAFIIGEEFIGDDPVCLVLGDNIFYGQSFSKTLKNAASRDLGATVFGYQVKDPERFGVVEFDEKMKAISIEEK
PLKPKSNYAVTGLYFYDNRVVEMAKQVKPSHRGELEITTLNEMYLNDGSLNVELLARGFAWLDTGTHESLHEASSFVQTI
EHIQGLKVACLEEISWRNGWLSDEQILDLATPMMKNEYGQYLTRLINDNKQKEL

Sequences:

>Translated_294_residues
MKGIVLAGGSGTRLYPLTRGVSKQLLPIYDKPMVFYPISTLMLAGIKDILIITTPEDNAGFKRLLGDGSDFGINLEYAIQ
PSPDGLAQAFIIGEEFIGDDPVCLVLGDNIFYGQSFSKTLKNAASRDLGATVFGYQVKDPERFGVVEFDEKMKAISIEEK
PLKPKSNYAVTGLYFYDNRVVEMAKQVKPSHRGELEITTLNEMYLNDGSLNVELLARGFAWLDTGTHESLHEASSFVQTI
EHIQGLKVACLEEISWRNGWLSDEQILDLATPMMKNEYGQYLTRLINDNKQKEL
>Mature_294_residues
MKGIVLAGGSGTRLYPLTRGVSKQLLPIYDKPMVFYPISTLMLAGIKDILIITTPEDNAGFKRLLGDGSDFGINLEYAIQ
PSPDGLAQAFIIGEEFIGDDPVCLVLGDNIFYGQSFSKTLKNAASRDLGATVFGYQVKDPERFGVVEFDEKMKAISIEEK
PLKPKSNYAVTGLYFYDNRVVEMAKQVKPSHRGELEITTLNEMYLNDGSLNVELLARGFAWLDTGTHESLHEASSFVQTI
EHIQGLKVACLEEISWRNGWLSDEQILDLATPMMKNEYGQYLTRLINDNKQKEL

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790224, Length=292, Percent_Identity=71.5753424657534, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1788351, Length=288, Percent_Identity=67.0138888888889, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI1787488, Length=241, Percent_Identity=24.0663900414938, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI133931050, Length=226, Percent_Identity=25.6637168141593, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320148, Length=193, Percent_Identity=26.4248704663212, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21355443, Length=229, Percent_Identity=25.764192139738, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24644084, Length=229, Percent_Identity=25.764192139738, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32816; Mature: 32816

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIVLAGGSGTRLYPLTRGVSKQLLPIYDKPMVFYPISTLMLAGIKDILIITTPEDNAG
CCCEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHCCCEEEEEECCCCCCC
FKRLLGDGSDFGINLEYAIQPSPDGLAQAFIIGEEFIGDDPVCLVLGDNIFYGQSFSKTL
HHHHHCCCCCCEEEEEEEECCCCCHHHHEEEECHHHCCCCCEEEEEECCEEECCHHHHHH
KNAASRDLGATVFGYQVKDPERFGVVEFDEKMKAISIEEKPLKPKSNYAVTGLYFYDNRV
HHHHCCCCCCEEEEEEECCCCCCCEEECCCCHHEEECCCCCCCCCCCEEEEEEEEECCHH
VEMAKQVKPSHRGELEITTLNEMYLNDGSLNVELLARGFAWLDTGTHESLHEASSFVQTI
HHHHHHCCCCCCCEEEEEEEHEEEECCCCEEEEEEECCCEEECCCCHHHHHHHHHHHHHH
EHIQGLKVACLEEISWRNGWLSDEQILDLATPMMKNEYGQYLTRLINDNKQKEL
HHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKGIVLAGGSGTRLYPLTRGVSKQLLPIYDKPMVFYPISTLMLAGIKDILIITTPEDNAG
CCCEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHCCCEEEEEECCCCCCC
FKRLLGDGSDFGINLEYAIQPSPDGLAQAFIIGEEFIGDDPVCLVLGDNIFYGQSFSKTL
HHHHHCCCCCCEEEEEEEECCCCCHHHHEEEECHHHCCCCCEEEEEECCEEECCHHHHHH
KNAASRDLGATVFGYQVKDPERFGVVEFDEKMKAISIEEKPLKPKSNYAVTGLYFYDNRV
HHHHCCCCCCEEEEEEECCCCCCCEEECCCCHHEEECCCCCCCCCCCEEEEEEEEECCHH
VEMAKQVKPSHRGELEITTLNEMYLNDGSLNVELLARGFAWLDTGTHESLHEASSFVQTI
HHHHHHCCCCCCCEEEEEEEHEEEECCCCEEEEEEECCCEEECCCCHHHHHHHHHHHHHH
EHIQGLKVACLEEISWRNGWLSDEQILDLATPMMKNEYGQYLTRLINDNKQKEL
HHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]