Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 209693699

GI number: 209693699

Start: 78654

End: 79163

Strand: Reverse

Name: def [H]

Synonym: VSAL_I0075

Alternate gene names: 209693699

Gene position: 79163-78654 (Counterclockwise)

Preceding gene: 209693704

Following gene: 209693698

Centisome position: 2.38

GC content: 40.2

Gene sequence:

>510_bases
ATGGCCGTATTAGAAGTATTAACGTTCCCGGACGACCGTCTACGCACCGTAGCAAAACCGGTAAAAACAGTAACACCTGA
AATCCAAACATTCATCGATGATATGATTGAAACCATGTATGACGAAGAAGGCATTGGCCTTGCTGCAACGCAAGTCGATT
TCCATCAACGTATCGTGGTTATCGATGTATCAGAGACACGCGATGAACCAATGGTATTAATCAATCCTGTTATTACACAA
AAAAGTGGTGATGACGGTATAGAAGAAGGTTGTTTATCTGTTCCTGGCGCAAAAGGAATGGTTCCTCGTTCTGCTGAAGT
GTCAGTAACGGCATTGGATCGTGATGGAAATGAATTTAGCTTTGATGCTGATGATTTACTAGCAATCTGCGTGCAACACG
AATTAGATCACCTTGAAGGTAAATTATTCGTTGATTACTTGTCGCCATTAAAGCGCAAACGCATCAAAGAGAAGCTAGAA
AAAATCAAAAAATTCAACGAAAAAAATTAG

Upstream 100 bases:

>100_bases
AGACATTCGAACCGATGCTGTCATTTGCTTAATAAAATGTCTAGAATTGGAGCTATTAGGTTTATGCCGTTTTCGGCGCA
GTTCAATATTTAGAGTAATT

Downstream 100 bases:

>100_bases
TCATGATGACCGAGGTTAACTTGAACAAACCCTTACGCATTATATTTGCAGGTACGCCTGATTTCGCGGCTCGCCATTTA
GCCGCTCTACTTGATTCTCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MAVLEVLTFPDDRLRTVAKPVKTVTPEIQTFIDDMIETMYDEEGIGLAATQVDFHQRIVVIDVSETRDEPMVLINPVITQ
KSGDDGIEEGCLSVPGAKGMVPRSAEVSVTALDRDGNEFSFDADDLLAICVQHELDHLEGKLFVDYLSPLKRKRIKEKLE
KIKKFNEKN

Sequences:

>Translated_169_residues
MAVLEVLTFPDDRLRTVAKPVKTVTPEIQTFIDDMIETMYDEEGIGLAATQVDFHQRIVVIDVSETRDEPMVLINPVITQ
KSGDDGIEEGCLSVPGAKGMVPRSAEVSVTALDRDGNEFSFDADDLLAICVQHELDHLEGKLFVDYLSPLKRKRIKEKLE
KIKKFNEKN
>Mature_168_residues
AVLEVLTFPDDRLRTVAKPVKTVTPEIQTFIDDMIETMYDEEGIGLAATQVDFHQRIVVIDVSETRDEPMVLINPVITQK
SGDDGIEEGCLSVPGAKGMVPRSAEVSVTALDRDGNEFSFDADDLLAICVQHELDHLEGKLFVDYLSPLKRKRIKEKLEK
IKKFNEKN

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=155, Percent_Identity=30.3225806451613, Blast_Score=74, Evalue=9e-14,
Organism=Escherichia coli, GI1789682, Length=168, Percent_Identity=64.2857142857143, Blast_Score=234, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24645726, Length=141, Percent_Identity=30.4964539007092, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18975; Mature: 18844

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLEVLTFPDDRLRTVAKPVKTVTPEIQTFIDDMIETMYDEEGIGLAATQVDFHQRIVV
CCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCEEEE
IDVSETRDEPMVLINPVITQKSGDDGIEEGCLSVPGAKGMVPRSAEVSVTALDRDGNEFS
EECCCCCCCCEEEECCEEECCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCC
FDADDLLAICVQHELDHLEGKLFVDYLSPLKRKRIKEKLEKIKKFNEKN
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AVLEVLTFPDDRLRTVAKPVKTVTPEIQTFIDDMIETMYDEEGIGLAATQVDFHQRIVV
CEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCEEEE
IDVSETRDEPMVLINPVITQKSGDDGIEEGCLSVPGAKGMVPRSAEVSVTALDRDGNEFS
EECCCCCCCCEEEECCEEECCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCC
FDADDLLAICVQHELDHLEGKLFVDYLSPLKRKRIKEKLEKIKKFNEKN
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA