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The map label for this gene is pinE [H]

Identifier: 197120039

GI number: 197120039

Start: 4206121

End: 4206693

Strand: Direct

Name: pinE [H]

Synonym: Gbem_3678

Alternate gene names: 197120039

Gene position: 4206121-4206693 (Clockwise)

Preceding gene: 197120037

Following gene: 197120048

Centisome position: 91.14

GC content: 57.24

Gene sequence:

>573_bases
ATGGCAATCGTAGGGTATGCAAGGGTGAGTTCGACAGACCAGAAGCTCGACGTGCAACTCGATCAACTCAAGGCACTGGG
CGTTGACAAGGTCTATGCTGAGAAGGCCAGCGGAGCCGATGCTGAACGGGTTGAGCTTGCCTCCCTTCTGGACTACGTGC
GCGAGGGTGACACCGTAGTGGCCTGCAAGCTCGACAGGATCGCCAGGAGCACACAGCACCTGCTTAACATCGTGGAGACG
TTGAACAAGAAGGGTGTCGCACTCAGGGTGCTGAACATCGACCTTGACACCTCTACCCCGACAGGGAAGCTCATGTTGAC
GATGCTGGGCGCTATCGCTACTTTCGAGAGAGAGATGATGCTTGAAAGGCAGGCTGACGGGATCGCCAAGGCTAAGGAGG
CCGGGAAGTACACGGGAAGGAAACCGACAGCCAAGGCAAAGAGCGCCGAGGTGGTAACCCTTCTGGGGCAAGGCAAGACG
AAAGAGGCGGTAGCCGCTGAACTGGGAATCGGCGTAGCAAGTGTCTACAGGATCGCCAAGGAGGAGAAGTTGAGGATGAC
CGCGCAGCCTTAA

Upstream 100 bases:

>100_bases
AAAAATGGGCTCCCATGCTATCACAGGAGTTGACTTCGGGGTTTTGATAGGTTATTATCAAAACATCTTAGACATCATTG
ATAGCAATCAGGAGGGCAGC

Downstream 100 bases:

>100_bases
AGAGCCAAGTCAGTTCAGTCATACAAAAGGCCGGGAGCAATCCCGGCCTTTTGCTATCTGAGACGACCCAGAATGCCCTA
CAAGCCACGATCTCGCGCCG

Product: integrative genetic element, resolvase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MAIVGYARVSSTDQKLDVQLDQLKALGVDKVYAEKASGADAERVELASLLDYVREGDTVVACKLDRIARSTQHLLNIVET
LNKKGVALRVLNIDLDTSTPTGKLMLTMLGAIATFEREMMLERQADGIAKAKEAGKYTGRKPTAKAKSAEVVTLLGQGKT
KEAVAAELGIGVASVYRIAKEEKLRMTAQP

Sequences:

>Translated_190_residues
MAIVGYARVSSTDQKLDVQLDQLKALGVDKVYAEKASGADAERVELASLLDYVREGDTVVACKLDRIARSTQHLLNIVET
LNKKGVALRVLNIDLDTSTPTGKLMLTMLGAIATFEREMMLERQADGIAKAKEAGKYTGRKPTAKAKSAEVVTLLGQGKT
KEAVAAELGIGVASVYRIAKEEKLRMTAQP
>Mature_189_residues
AIVGYARVSSTDQKLDVQLDQLKALGVDKVYAEKASGADAERVELASLLDYVREGDTVVACKLDRIARSTQHLLNIVETL
NKKGVALRVLNIDLDTSTPTGKLMLTMLGAIATFEREMMLERQADGIAKAKEAGKYTGRKPTAKAKSAEVVTLLGQGKTK
EAVAAELGIGVASVYRIAKEEKLRMTAQP

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=176, Percent_Identity=36.9318181818182, Blast_Score=131, Evalue=2e-32,
Organism=Escherichia coli, GI1787638, Length=183, Percent_Identity=31.1475409836066, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787827, Length=183, Percent_Identity=30.6010928961749, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 20477; Mature: 20346

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVGYARVSSTDQKLDVQLDQLKALGVDKVYAEKASGADAERVELASLLDYVREGDTVV
CEEEECEECCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEE
ACKLDRIARSTQHLLNIVETLNKKGVALRVLNIDLDTSTPTGKLMLTMLGAIATFEREMM
EEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
LERQADGIAKAKEAGKYTGRKPTAKAKSAEVVTLLGQGKTKEAVAAELGIGVASVYRIAK
HHHHHCHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCHHHHHHHHHH
EEKLRMTAQP
HHHHCCCCCC
>Mature Secondary Structure 
AIVGYARVSSTDQKLDVQLDQLKALGVDKVYAEKASGADAERVELASLLDYVREGDTVV
EEEECEECCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEE
ACKLDRIARSTQHLLNIVETLNKKGVALRVLNIDLDTSTPTGKLMLTMLGAIATFEREMM
EEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
LERQADGIAKAKEAGKYTGRKPTAKAKSAEVVTLLGQGKTKEAVAAELGIGVASVYRIAK
HHHHHCHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCHHHHHHHHHH
EEKLRMTAQP
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]