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The map label for this gene is gatB

Identifier: 197120006

GI number: 197120006

Start: 4169547

End: 4171013

Strand: Direct

Name: gatB

Synonym: Gbem_3645

Alternate gene names: 197120006

Gene position: 4169547-4171013 (Clockwise)

Preceding gene: 197120005

Following gene: 197120007

Centisome position: 90.34

GC content: 62.58

Gene sequence:

>1467_bases
ATGAAATATCAGGTCGTCATCGGGCTCGAGGTCCACACCCAGCTCACCACCAACACCAAGATCTTCTGCGGCTGCTCGAC
CCGCTTCGGGGCGGAGCCGAACTCCCAGACCTGCCCGGTCTGCCTCGGCTTACCAGGCGCGCTCCCGGTACTGAACCAGC
AGGTGGTGGAATACGCCATCCGCGCCGGACTCGCCACCAACTGCTCCATCACCAAAAGGAACGTCTTCGCCCGGAAAAAC
TACTTCTACCCTGACCTCCCGAAGGGTTACCAGATCAGCCAGTTCGACCTCCCCATCTGCCAGCACGGCCACCTGGACAT
CGAGGTGGAGGGCGAAAAGAAACGGATCGGGATCACCCGCATCCACATGGAGGAGGACGCCGGCAAGTTGGTGCACGCTG
ACATCCCCGGGGTGGACGATTCCTGCGTCGACCTGAACCGCGCCTGCACGCCGCTACTGGAGATCGTCTCGGAGCCGGAC
ATGCGCTCCCCGGACGAGGCAATCGCCTACCTGAAGAAGCTGCACCAGATCGTCATGTATCTGGGGATCTGCGACGGCAA
CCTGGAGGAAGGGAGCTTCCGCTGCGACGCGAACGTATCGCTCATGCCGGTCGGCTCCAACGTCTTCGGCACCCGCGCGG
AACTGAAGAACATCAACTCCTTCAAGTTCATCAAGCAGGCAATCGAGTACGAGATTGAGCGCCAGGCCGAGATCCTCGAC
GACGGCGGCAAGGTGGTCCAGGAAACCCGACTTTTCGACCCGAACACCGGCACCACCCGCTCCATGCGCGGCAAGGAGGA
GGCGCACGACTACCGCTACTTCCCGGACCCCGACCTGGTCCCGGTGATCATCTCGGACGACTGGATCGAGAAGGTGCGCG
GCGGGCTGCCCGAGCTTCCCGAGGCGAAGCGCGCGCGCTTCATGTCGGAAGGAGGGGCCGTATTTGGCGGCTCTGCATTC
GGACTCCCCGAGTACGACGCCGAGGTCCTGGTGGCAAGCCGCGAGCTGGCGCAGTACTTCGAGGACACGGTCGCGCTCTT
CCCGCAGGCCAAGACCGTCTCCAACTGGGTCATGGGCGAAGTCACCCGCGCCCTCAACGACGACAACAACCGCTCCATCG
CCGAGTGCCCGGTCACCCCGGCTCTCTTGGCCGACCTCCTGAAGCTGATGGAGAAAGGGACCATCTCCGGCAAGATCGCC
AAGACCGTCTTCGACGAGATGTACAAGACCGGCAAGGCGTCGGAGAAGATCGTCGAGGAGAAGGGTCTGGTGCAGGTCTC
CGACACCGGAGCCATCGAGGCCATGGTGGACGAGGTGCTGGAGAAGAACCCCGGGCAGGTAGCCGAGTACAGGGGTGGCA
AAGAGACCCTTTTCGGCTTCTTCGTGGGCCAAGTCATGCGCGCCTCCAAAGGGAAGGCGAACCCCGCCGTCGTAAACGAA
CTGCTGCTAAAGAAACTCAACAGCTAA

Upstream 100 bases:

>100_bases
CAAAGCCTTTAGCGGTTAAGACCAAAAGCCGTTGTTTGGTTTGCTCAGATTTCCTCAGATTTTCTCCTTGCTAACGGTTT
TAGGTTTTTTGGAGCTATCT

Downstream 100 bases:

>100_bases
CTACGACACCATCTTTCAGACCGAACAACCTGCTCCCCCCCCTCCCTTGACGGGAGGGGGTCGGGGGGTGGGTGAAGCTG
CCACCGGATCAGCCGTGGCG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MKYQVVIGLEVHTQLTTNTKIFCGCSTRFGAEPNSQTCPVCLGLPGALPVLNQQVVEYAIRAGLATNCSITKRNVFARKN
YFYPDLPKGYQISQFDLPICQHGHLDIEVEGEKKRIGITRIHMEEDAGKLVHADIPGVDDSCVDLNRACTPLLEIVSEPD
MRSPDEAIAYLKKLHQIVMYLGICDGNLEEGSFRCDANVSLMPVGSNVFGTRAELKNINSFKFIKQAIEYEIERQAEILD
DGGKVVQETRLFDPNTGTTRSMRGKEEAHDYRYFPDPDLVPVIISDDWIEKVRGGLPELPEAKRARFMSEGGAVFGGSAF
GLPEYDAEVLVASRELAQYFEDTVALFPQAKTVSNWVMGEVTRALNDDNNRSIAECPVTPALLADLLKLMEKGTISGKIA
KTVFDEMYKTGKASEKIVEEKGLVQVSDTGAIEAMVDEVLEKNPGQVAEYRGGKETLFGFFVGQVMRASKGKANPAVVNE
LLLKKLNS

Sequences:

>Translated_488_residues
MKYQVVIGLEVHTQLTTNTKIFCGCSTRFGAEPNSQTCPVCLGLPGALPVLNQQVVEYAIRAGLATNCSITKRNVFARKN
YFYPDLPKGYQISQFDLPICQHGHLDIEVEGEKKRIGITRIHMEEDAGKLVHADIPGVDDSCVDLNRACTPLLEIVSEPD
MRSPDEAIAYLKKLHQIVMYLGICDGNLEEGSFRCDANVSLMPVGSNVFGTRAELKNINSFKFIKQAIEYEIERQAEILD
DGGKVVQETRLFDPNTGTTRSMRGKEEAHDYRYFPDPDLVPVIISDDWIEKVRGGLPELPEAKRARFMSEGGAVFGGSAF
GLPEYDAEVLVASRELAQYFEDTVALFPQAKTVSNWVMGEVTRALNDDNNRSIAECPVTPALLADLLKLMEKGTISGKIA
KTVFDEMYKTGKASEKIVEEKGLVQVSDTGAIEAMVDEVLEKNPGQVAEYRGGKETLFGFFVGQVMRASKGKANPAVVNE
LLLKKLNS
>Mature_488_residues
MKYQVVIGLEVHTQLTTNTKIFCGCSTRFGAEPNSQTCPVCLGLPGALPVLNQQVVEYAIRAGLATNCSITKRNVFARKN
YFYPDLPKGYQISQFDLPICQHGHLDIEVEGEKKRIGITRIHMEEDAGKLVHADIPGVDDSCVDLNRACTPLLEIVSEPD
MRSPDEAIAYLKKLHQIVMYLGICDGNLEEGSFRCDANVSLMPVGSNVFGTRAELKNINSFKFIKQAIEYEIERQAEILD
DGGKVVQETRLFDPNTGTTRSMRGKEEAHDYRYFPDPDLVPVIISDDWIEKVRGGLPELPEAKRARFMSEGGAVFGGSAF
GLPEYDAEVLVASRELAQYFEDTVALFPQAKTVSNWVMGEVTRALNDDNNRSIAECPVTPALLADLLKLMEKGTISGKIA
KTVFDEMYKTGKASEKIVEEKGLVQVSDTGAIEAMVDEVLEKNPGQVAEYRGGKETLFGFFVGQVMRASKGKANPAVVNE
LLLKKLNS

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=508, Percent_Identity=37.992125984252, Blast_Score=328, Evalue=5e-90,
Organism=Caenorhabditis elegans, GI115534094, Length=303, Percent_Identity=40.5940594059406, Blast_Score=215, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6319391, Length=519, Percent_Identity=29.6724470134875, Blast_Score=213, Evalue=8e-56,
Organism=Drosophila melanogaster, GI45550808, Length=504, Percent_Identity=32.5396825396825, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_GEOBB (B5ED20)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002140433.1
- ProteinModelPortal:   B5ED20
- SMR:   B5ED20
- GeneID:   6780509
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_3645
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53859; Mature: 53859

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYQVVIGLEVHTQLTTNTKIFCGCSTRFGAEPNSQTCPVCLGLPGALPVLNQQVVEYAI
CCEEEEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
RAGLATNCSITKRNVFARKNYFYPDLPKGYQISQFDLPICQHGHLDIEVEGEKKRIGITR
HCCCCCCCCEEHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCEEEEECCCCCEECEEE
IHMEEDAGKLVHADIPGVDDSCVDLNRACTPLLEIVSEPDMRSPDEAIAYLKKLHQIVMY
EEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LGICDGNLEEGSFRCDANVSLMPVGSNVFGTRAELKNINSFKFIKQAIEYEIERQAEILD
HHCCCCCCCCCCEEECCCEEEEECCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
DGGKVVQETRLFDPNTGTTRSMRGKEEAHDYRYFPDPDLVPVIISDDWIEKVRGGLPELP
CCCHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCHHHHHHHCCCCCCC
EAKRARFMSEGGAVFGGSAFGLPEYDAEVLVASRELAQYFEDTVALFPQAKTVSNWVMGE
HHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VTRALNDDNNRSIAECPVTPALLADLLKLMEKGTISGKIAKTVFDEMYKTGKASEKIVEE
HHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
KGLVQVSDTGAIEAMVDEVLEKNPGQVAEYRGGKETLFGFFVGQVMRASKGKANPAVVNE
CCCEEEECCCHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
LLLKKLNS
HHHHHHCC
>Mature Secondary Structure
MKYQVVIGLEVHTQLTTNTKIFCGCSTRFGAEPNSQTCPVCLGLPGALPVLNQQVVEYAI
CCEEEEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
RAGLATNCSITKRNVFARKNYFYPDLPKGYQISQFDLPICQHGHLDIEVEGEKKRIGITR
HCCCCCCCCEEHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCEEEEECCCCCEECEEE
IHMEEDAGKLVHADIPGVDDSCVDLNRACTPLLEIVSEPDMRSPDEAIAYLKKLHQIVMY
EEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LGICDGNLEEGSFRCDANVSLMPVGSNVFGTRAELKNINSFKFIKQAIEYEIERQAEILD
HHCCCCCCCCCCEEECCCEEEEECCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
DGGKVVQETRLFDPNTGTTRSMRGKEEAHDYRYFPDPDLVPVIISDDWIEKVRGGLPELP
CCCHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCHHHHHHHCCCCCCC
EAKRARFMSEGGAVFGGSAFGLPEYDAEVLVASRELAQYFEDTVALFPQAKTVSNWVMGE
HHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VTRALNDDNNRSIAECPVTPALLADLLKLMEKGTISGKIAKTVFDEMYKTGKASEKIVEE
HHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
KGLVQVSDTGAIEAMVDEVLEKNPGQVAEYRGGKETLFGFFVGQVMRASKGKANPAVVNE
CCCEEEECCCHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
LLLKKLNS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA