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The map label for this gene is gatA

Identifier: 197120005

GI number: 197120005

Start: 4167914

End: 4169371

Strand: Direct

Name: gatA

Synonym: Gbem_3644

Alternate gene names: 197120005

Gene position: 4167914-4169371 (Clockwise)

Preceding gene: 197120004

Following gene: 197120006

Centisome position: 90.31

GC content: 64.13

Gene sequence:

>1458_bases
ATGGAAATTTTCGACCTAACCATACACGAGCTGCATGAGAAGCTGAAGGCGAAGGAGGTCTCTTCCGTCGAGGCGACCCG
CGCCATGCTGGACCGGATCGAGGCGGTGGACAGCCAGGTGAACGCCTACATCACCGTGACCCCGGAGCAGGCGCTGGTAC
AGGCTGAGGACGCGGACCGCCGCATCGCCGCTGGCGAAATCGCGCCTCTGACCGGCATCCCGGTCGGCCTCAAGGACATA
TTCGTCACCAAGGGAATCCGCACCACCTGCGGCTCGCGCATCCTGGAAAACTTCGTCCCCCCCTACGACGGCACAGCGGT
AGCAAAGCTTAAAGAGCAGGGCGCGGTCATCGTCGGCAAGCTGAACCAGGACGAGTTCGCCATGGGCTCCTCCAACGAGT
CGAGCTACTTCGGCCCCTGCAAGAACCCGTGGGATCTTTCCTGCACGCCGGGCGGCTCCTCCGGGGGCTCCGCCGCCGCC
ATCGCCGCACGCACCGCGACCGCGACGCTCGGAACCGATACCGGCGGCTCGATACGCCAACCGGCCTCGCACTGCGGCTG
CGTGGGCCTGAAGCCGACCTACGGCAGGGTCTCCCGCTACGGCGTCATCGCTTACGCCTCCTCGCTGGATCAGGTAGGCC
CGCTCACCCGCGACGTTACCGACTCGGCACTCCTTTTGGGAGCGGTGGCAGGTTACGACCCGATGGATTCCACCTCGGTC
AACACCCCGGTCCCCGACTACGTCGCCTCCCTCGCCAAAGGCGTAAAGGGTATAAAGATCGGCCTTCCCAAGCAGTACTT
CATCGAGGGTCTCGACCCGGACGTGAAGCGCGCCATGGACGAGGCGATCGCCATGTACAAGAGCCTCGGCGCCGACATTC
GTGAAGTGAGCCTCCCCAACACCGAGTACGCCGTCGCCACCTACTACATCATCGCCACCGCAGAGGCTAGCGCCAACTTG
GCCCGCTACGACGGCGTCCGCTTCGGGCACCGCGCAGAGGATGCCAGGGGCCTGGCGCAGATGTACTCCAAGAGCCGCGC
CGAGGGGTTCGGCCCCGAGGTTAAGCGCCGCATCATGTTGGGAACCTACGCCCTTTCCTCCGGCTACTACGACGCCTACT
ACGTCAAGGCGCAGAAGGTGCGCACCCTGATCCAGCAGGACTTCCTGAACGCCTTTAAGGAAGTCGACGTGCTGTTGACC
CCGATAGCGCCCACCCCGGCCTTCAAGATCGCGGAAAAGCTCGAGGACCCGCTGCAGATGTACCTCTCCGACATCTTCAC
CATTCCGGTGAACCTGGCCGGAACCTGCGGCATCTCCGTTCCCGCCGGGTTCAGCGCCGCAGGACTTCCCATCGGGCTGC
AACTGGTAGGGAAACCGTTCGGCGAGCAGGAAATCCTCACCGCCGCGTACTCCTTCGAAAGGGAGACGCAGTGGCACCTG
AAGAAGGCGCCGCTGTAA

Upstream 100 bases:

>100_bases
TTCTTCAGGGTTCCCAAGGTCATCGAGTAAAGGCAGTCTGAGTCTGACAGGTCTGACAGCTGAGACTGACCACAAAATCG
CTCAACACCGGAGTTTTGCA

Downstream 100 bases:

>100_bases
AACAAAGTCAAAACCATTGGCACGGAGAACGTCTGAGGACTTCTGAGAAAACCGTGAACCAGAGAATTTAAGACTCAAAG
CCTTTAGCGGTTAAGACCAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MEIFDLTIHELHEKLKAKEVSSVEATRAMLDRIEAVDSQVNAYITVTPEQALVQAEDADRRIAAGEIAPLTGIPVGLKDI
FVTKGIRTTCGSRILENFVPPYDGTAVAKLKEQGAVIVGKLNQDEFAMGSSNESSYFGPCKNPWDLSCTPGGSSGGSAAA
IAARTATATLGTDTGGSIRQPASHCGCVGLKPTYGRVSRYGVIAYASSLDQVGPLTRDVTDSALLLGAVAGYDPMDSTSV
NTPVPDYVASLAKGVKGIKIGLPKQYFIEGLDPDVKRAMDEAIAMYKSLGADIREVSLPNTEYAVATYYIIATAEASANL
ARYDGVRFGHRAEDARGLAQMYSKSRAEGFGPEVKRRIMLGTYALSSGYYDAYYVKAQKVRTLIQQDFLNAFKEVDVLLT
PIAPTPAFKIAEKLEDPLQMYLSDIFTIPVNLAGTCGISVPAGFSAAGLPIGLQLVGKPFGEQEILTAAYSFERETQWHL
KKAPL

Sequences:

>Translated_485_residues
MEIFDLTIHELHEKLKAKEVSSVEATRAMLDRIEAVDSQVNAYITVTPEQALVQAEDADRRIAAGEIAPLTGIPVGLKDI
FVTKGIRTTCGSRILENFVPPYDGTAVAKLKEQGAVIVGKLNQDEFAMGSSNESSYFGPCKNPWDLSCTPGGSSGGSAAA
IAARTATATLGTDTGGSIRQPASHCGCVGLKPTYGRVSRYGVIAYASSLDQVGPLTRDVTDSALLLGAVAGYDPMDSTSV
NTPVPDYVASLAKGVKGIKIGLPKQYFIEGLDPDVKRAMDEAIAMYKSLGADIREVSLPNTEYAVATYYIIATAEASANL
ARYDGVRFGHRAEDARGLAQMYSKSRAEGFGPEVKRRIMLGTYALSSGYYDAYYVKAQKVRTLIQQDFLNAFKEVDVLLT
PIAPTPAFKIAEKLEDPLQMYLSDIFTIPVNLAGTCGISVPAGFSAAGLPIGLQLVGKPFGEQEILTAAYSFERETQWHL
KKAPL
>Mature_485_residues
MEIFDLTIHELHEKLKAKEVSSVEATRAMLDRIEAVDSQVNAYITVTPEQALVQAEDADRRIAAGEIAPLTGIPVGLKDI
FVTKGIRTTCGSRILENFVPPYDGTAVAKLKEQGAVIVGKLNQDEFAMGSSNESSYFGPCKNPWDLSCTPGGSSGGSAAA
IAARTATATLGTDTGGSIRQPASHCGCVGLKPTYGRVSRYGVIAYASSLDQVGPLTRDVTDSALLLGAVAGYDPMDSTSV
NTPVPDYVASLAKGVKGIKIGLPKQYFIEGLDPDVKRAMDEAIAMYKSLGADIREVSLPNTEYAVATYYIIATAEASANL
ARYDGVRFGHRAEDARGLAQMYSKSRAEGFGPEVKRRIMLGTYALSSGYYDAYYVKAQKVRTLIQQDFLNAFKEVDVLLT
PIAPTPAFKIAEKLEDPLQMYLSDIFTIPVNLAGTCGISVPAGFSAAGLPIGLQLVGKPFGEQEILTAAYSFERETQWHL
KKAPL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=42.3694779116466, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI195972892, Length=502, Percent_Identity=24.3027888446215, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI166795287, Length=156, Percent_Identity=43.5897435897436, Blast_Score=80, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=439, Percent_Identity=39.6355353075171, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17537465, Length=492, Percent_Identity=25, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17556264, Length=316, Percent_Identity=28.7974683544304, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=325, Percent_Identity=28, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=309, Percent_Identity=30.42071197411, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17556278, Length=309, Percent_Identity=30.42071197411, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=319, Percent_Identity=26.9592476489028, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17538254, Length=521, Percent_Identity=21.8809980806142, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=456, Percent_Identity=33.9912280701754, Blast_Score=239, Evalue=7e-64,
Organism=Saccharomyces cerevisiae, GI6319685, Length=477, Percent_Identity=29.3501048218029, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320448, Length=229, Percent_Identity=34.9344978165939, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648113, Length=456, Percent_Identity=43.2017543859649, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI161078093, Length=504, Percent_Identity=29.5634920634921, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24644968, Length=502, Percent_Identity=26.8924302788845, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI21356731, Length=494, Percent_Identity=26.5182186234818, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24652985, Length=489, Percent_Identity=24.9488752556237, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI19922090, Length=489, Percent_Identity=24.9488752556237, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24652981, Length=489, Percent_Identity=24.9488752556237, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24652983, Length=489, Percent_Identity=24.9488752556237, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI45550774, Length=498, Percent_Identity=25.5020080321285, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24648435, Length=498, Percent_Identity=25.5020080321285, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24648437, Length=498, Percent_Identity=25.5020080321285, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24648441, Length=435, Percent_Identity=25.5172413793103, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648439, Length=435, Percent_Identity=25.5172413793103, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_GEOBB (B5ED19)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002140432.1
- ProteinModelPortal:   B5ED19
- SMR:   B5ED19
- GeneID:   6780508
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_3644
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51951; Mature: 51951

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 153-153 ACT_SITE 177-177

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIFDLTIHELHEKLKAKEVSSVEATRAMLDRIEAVDSQVNAYITVTPEQALVQAEDADR
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCCC
RIAAGEIAPLTGIPVGLKDIFVTKGIRTTCGSRILENFVPPYDGTAVAKLKEQGAVIVGK
EEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEE
LNQDEFAMGSSNESSYFGPCKNPWDLSCTPGGSSGGSAAAIAARTATATLGTDTGGSIRQ
CCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEEHHHEEEECCCCCCCCCC
PASHCGCVGLKPTYGRVSRYGVIAYASSLDQVGPLTRDVTDSALLLGAVAGYDPMDSTSV
CHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
NTPVPDYVASLAKGVKGIKIGLPKQYFIEGLDPDVKRAMDEAIAMYKSLGADIREVSLPN
CCCCHHHHHHHHCCCCCEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCC
TEYAVATYYIIATAEASANLARYDGVRFGHRAEDARGLAQMYSKSRAEGFGPEVKRRIML
CCEEEEEEEEEEECCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
GTYALSSGYYDAYYVKAQKVRTLIQQDFLNAFKEVDVLLTPIAPTPAFKIAEKLEDPLQM
EEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHH
YLSDIFTIPVNLAGTCGISVPAGFSAAGLPIGLQLVGKPFGEQEILTAAYSFERETQWHL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHH
KKAPL
CCCCC
>Mature Secondary Structure
MEIFDLTIHELHEKLKAKEVSSVEATRAMLDRIEAVDSQVNAYITVTPEQALVQAEDADR
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCCC
RIAAGEIAPLTGIPVGLKDIFVTKGIRTTCGSRILENFVPPYDGTAVAKLKEQGAVIVGK
EEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEE
LNQDEFAMGSSNESSYFGPCKNPWDLSCTPGGSSGGSAAAIAARTATATLGTDTGGSIRQ
CCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEEHHHEEEECCCCCCCCCC
PASHCGCVGLKPTYGRVSRYGVIAYASSLDQVGPLTRDVTDSALLLGAVAGYDPMDSTSV
CHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
NTPVPDYVASLAKGVKGIKIGLPKQYFIEGLDPDVKRAMDEAIAMYKSLGADIREVSLPN
CCCCHHHHHHHHCCCCCEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCC
TEYAVATYYIIATAEASANLARYDGVRFGHRAEDARGLAQMYSKSRAEGFGPEVKRRIML
CCEEEEEEEEEEECCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
GTYALSSGYYDAYYVKAQKVRTLIQQDFLNAFKEVDVLLTPIAPTPAFKIAEKLEDPLQM
EEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHH
YLSDIFTIPVNLAGTCGISVPAGFSAAGLPIGLQLVGKPFGEQEILTAAYSFERETQWHL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHH
KKAPL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA