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The map label for this gene is alaS [H]

Identifier: 197119994

GI number: 197119994

Start: 4154577

End: 4157204

Strand: Direct

Name: alaS [H]

Synonym: Gbem_3633

Alternate gene names: 197119994

Gene position: 4154577-4157204 (Clockwise)

Preceding gene: 197119993

Following gene: 197119995

Centisome position: 90.02

GC content: 65.03

Gene sequence:

>2628_bases
ATGACAGGCAAAGAGATCCGGGCTCAGTTCTTCAACTTCTTTCAGAAAAAAGGGCATGCCCTTGTGGAGAGCTCGAACCT
CATCCCACGCAACGACCCTACCCTCCTCTTCACCAACGCCGGCATGAACCAGTTCAAGGACGTCTTCCTCGGCATGGAGA
AAAGGGATTACCTGAGGGCGGTCTCCTCCCAGAAATGCGTGCGCGCCGGCGGCAAGCACAACGACCTCGAGAACGTGGGT
CGCACCGCCAGGCATCACACCTTTTTCGAGATGCTGGGGAATTTCTCCTTCGGCGACTATTTCAAGAAGGAGGCGATCGG
CTTTGCCTGGGAATTCCTGACCAAGGAACTCGGCCTCTCCAAGGACCGCCTCTACGTCACCGTCTACACCGACGACGACG
AAGCGGCCGACATCTGGCACAACCAGGAAGGGGTGCCGCGCGAGCGCATCTACCGCTTCGGCGAGAAGGACAACTTCTGG
TCCATGGGCGACACCGGCCCCTGCGGCCCCTGCACCGAGATCTTCTGGGACAACGGCCCGGGAACCGGCTGCGGCAGCCC
CGACTGCGCGGTAGGATGCGATTGCGACCGCTACATGGAGATCTGGAACAACGTCTTCATGCAGTTCAACCGCGGCAAGG
ACGGCGTCCTGACCCCGCTCCCGAAACCTTCCGTCGACACCGGCATGGGCCTTGAGCGCATCTCGGCCGTCATGCAGGGG
GTCACCTCCAACTACGACACGGACCTCCTGCAGGGGATCATCCGCCACATCGAGCAGGTCTCCGGGAAGAAGTACCGGGA
CGACGAGAAGGATGACGTCTCCATGCGCGTCATCGCCGACCACGCCCGCGCCGTCACCTTCCTGATCTGCGACGGCGTCC
TCCCCAGCAACGAGGGGCGCGGCTACGTGCTCAGGCGCATCATGCGCCGCGCCGCCCGCCACGCGAAGATGCTGGGAATC
GCCGAGCCGGTCCTCTTCCGCATCGTCGACGCGGTGAACATGATGATGGGCGACGCCTACCCGGAGCTTCTGGAGCGCGA
GCATTACGTAAAGAAGGTGATCCTGGCCGAGGAGGAGCGCTTCATCGAGACCCTCGACCGCGGCCTCGCCATCTTGAACG
AGGAGACGGCGGCGCTCAAGGCCAAGGGAGAGAAGGTCATCTCCGGCGAGGTCATCTTCAAGCTCTACGACACCTTCGGC
TTCCCGGTCGACTTGACCGCCGACATCGTCGAGTCCGAAGGATTCACCCTCGACGAGGACGGCTTCGCCCTCTGCATGGA
GAAGCAGCGCGTCAAAGCGAGGGAGAACTGGAAGGGGTCCGGCGAGCAGGGGCTGGCCGACATCTACAAGGAGCTGCACG
GCACCGGCATCACCACCGACTTCCTGGGCTACAGCGAGCAGACCGCCTTCTCCACCATCAGCGCCATCGTCAAGGGTGGG
ACCCTGGTCGAAGAGGCGAGCGCCGGCGACGAGATCGAGATCGTCACCGCGGCGACCCCGTTCTACGGCGAGTCCGGCGG
GCAGGCCGGCGACACCGGAACCATCTCCACCGGCTCCGGCCACATCGAAGTGACCGGCTCCACCCGCCCCTTCACCGACC
TCATCGTGCACCGCGGCCGCGTGGTCTCCGGCGCCGTCAGGACCGGCGAAGCGGCAGACCTGAAGATCACCGTCGCCAAC
CGCGCCGCAACGGCCAGAAACCACACGGCGACCCACCTCTTGCAGGCGGCGCTCCGGGAGGTGCTGGGCGATCACGTGAA
GCAGGCAGGCTCCCTGGTGACCCCGGACCGGCTCCGCTTCGACTTCACCCACTTCACCCCGATGACCCAGGAGGAGATCC
GCAGGGTCGAGATCCTGGTGAACGGCCACATCATGGGGAACTCCACGGTTGAGTCCCGCGAGATGCCGGCGGCAGACGCG
CTTGCCGCAGGCGCCACAGCGCTCTTCGGCGAGAAGTACGGCGACGTGGTCCGCGTGGTCAAGGTGGGAGACGTCTCCAG
CGAACTCTGCGGCGGCACCCACGTCCACGGCGCCGGCGAGATCGGCTTCTTCAAGATCATCTCCGAGGCGGGGATCGCCG
CGGGGGTCCGGAGGATCGAGGCGCAGACCGGCAGCGGCGCGCTGGCGGTGGTGCACGGAATGGAAGACGAGCAGCGCGGC
ATCGCCACCCTTCTGAAGGCTGAAGGGGGAACCGTCCTGGACAAGGTCGAGAAACTGGTGGCCGGCCAGCGCGAGCTGCA
AAAGGAGCTCGAAACGCTGCAGGCGCGTCTGAACGCCTCCAAGTCCGCAGACCTGATCCAGCAGGTGCGCGAGCAAAACG
GCATCAAGATCCTGTCGGTAAAGGTCGACGGGGACGCTAAGGGGTTGAGGGAACTCTCCGACACGCTGAAGCAGCGCATC
GGCTCCGGCATCATCGTCCTTGGGACCAGCGACGCCGCGAGGGCGAACCTCCTGGTGGCGGTGACGGCCGATCTCTCGGC
GCGCTGGAAGGCGGGGGACATCATCAAGGCCATCGCCCCGATAGTCGGGGGCAACGGCGGAGGAAAGCCCGAGCTGGCGC
AGGCGGGAGGCTCCAAGCCGGAACACCTGGCCGAGGCGCTAGAGGCGGTGTACCAGATAGTGGGGTAA

Upstream 100 bases:

>100_bases
CAGGGGGAGAGGGAGTATGGACGAATTTAATCCCCAAGCCCTGTACAGAGGGAGCGGGGAATTTGAAGATGACCATTTAA
TTCCATTTTGAGGTTTTTTT

Downstream 100 bases:

>100_bases
AAACTCCCCCCTCCTGACCTCCCCCCTCTTAACCAGTCCCCTCCCCTGGAGGGGGAGGGTTAGGGAGGGGGAAGCGCTGC
GGCCCTTTCGACCAAAGCAA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 875; Mature: 874

Protein sequence:

>875_residues
MTGKEIRAQFFNFFQKKGHALVESSNLIPRNDPTLLFTNAGMNQFKDVFLGMEKRDYLRAVSSQKCVRAGGKHNDLENVG
RTARHHTFFEMLGNFSFGDYFKKEAIGFAWEFLTKELGLSKDRLYVTVYTDDDEAADIWHNQEGVPRERIYRFGEKDNFW
SMGDTGPCGPCTEIFWDNGPGTGCGSPDCAVGCDCDRYMEIWNNVFMQFNRGKDGVLTPLPKPSVDTGMGLERISAVMQG
VTSNYDTDLLQGIIRHIEQVSGKKYRDDEKDDVSMRVIADHARAVTFLICDGVLPSNEGRGYVLRRIMRRAARHAKMLGI
AEPVLFRIVDAVNMMMGDAYPELLEREHYVKKVILAEEERFIETLDRGLAILNEETAALKAKGEKVISGEVIFKLYDTFG
FPVDLTADIVESEGFTLDEDGFALCMEKQRVKARENWKGSGEQGLADIYKELHGTGITTDFLGYSEQTAFSTISAIVKGG
TLVEEASAGDEIEIVTAATPFYGESGGQAGDTGTISTGSGHIEVTGSTRPFTDLIVHRGRVVSGAVRTGEAADLKITVAN
RAATARNHTATHLLQAALREVLGDHVKQAGSLVTPDRLRFDFTHFTPMTQEEIRRVEILVNGHIMGNSTVESREMPAADA
LAAGATALFGEKYGDVVRVVKVGDVSSELCGGTHVHGAGEIGFFKIISEAGIAAGVRRIEAQTGSGALAVVHGMEDEQRG
IATLLKAEGGTVLDKVEKLVAGQRELQKELETLQARLNASKSADLIQQVREQNGIKILSVKVDGDAKGLRELSDTLKQRI
GSGIIVLGTSDAARANLLVAVTADLSARWKAGDIIKAIAPIVGGNGGGKPELAQAGGSKPEHLAEALEAVYQIVG

Sequences:

>Translated_875_residues
MTGKEIRAQFFNFFQKKGHALVESSNLIPRNDPTLLFTNAGMNQFKDVFLGMEKRDYLRAVSSQKCVRAGGKHNDLENVG
RTARHHTFFEMLGNFSFGDYFKKEAIGFAWEFLTKELGLSKDRLYVTVYTDDDEAADIWHNQEGVPRERIYRFGEKDNFW
SMGDTGPCGPCTEIFWDNGPGTGCGSPDCAVGCDCDRYMEIWNNVFMQFNRGKDGVLTPLPKPSVDTGMGLERISAVMQG
VTSNYDTDLLQGIIRHIEQVSGKKYRDDEKDDVSMRVIADHARAVTFLICDGVLPSNEGRGYVLRRIMRRAARHAKMLGI
AEPVLFRIVDAVNMMMGDAYPELLEREHYVKKVILAEEERFIETLDRGLAILNEETAALKAKGEKVISGEVIFKLYDTFG
FPVDLTADIVESEGFTLDEDGFALCMEKQRVKARENWKGSGEQGLADIYKELHGTGITTDFLGYSEQTAFSTISAIVKGG
TLVEEASAGDEIEIVTAATPFYGESGGQAGDTGTISTGSGHIEVTGSTRPFTDLIVHRGRVVSGAVRTGEAADLKITVAN
RAATARNHTATHLLQAALREVLGDHVKQAGSLVTPDRLRFDFTHFTPMTQEEIRRVEILVNGHIMGNSTVESREMPAADA
LAAGATALFGEKYGDVVRVVKVGDVSSELCGGTHVHGAGEIGFFKIISEAGIAAGVRRIEAQTGSGALAVVHGMEDEQRG
IATLLKAEGGTVLDKVEKLVAGQRELQKELETLQARLNASKSADLIQQVREQNGIKILSVKVDGDAKGLRELSDTLKQRI
GSGIIVLGTSDAARANLLVAVTADLSARWKAGDIIKAIAPIVGGNGGGKPELAQAGGSKPEHLAEALEAVYQIVG
>Mature_874_residues
TGKEIRAQFFNFFQKKGHALVESSNLIPRNDPTLLFTNAGMNQFKDVFLGMEKRDYLRAVSSQKCVRAGGKHNDLENVGR
TARHHTFFEMLGNFSFGDYFKKEAIGFAWEFLTKELGLSKDRLYVTVYTDDDEAADIWHNQEGVPRERIYRFGEKDNFWS
MGDTGPCGPCTEIFWDNGPGTGCGSPDCAVGCDCDRYMEIWNNVFMQFNRGKDGVLTPLPKPSVDTGMGLERISAVMQGV
TSNYDTDLLQGIIRHIEQVSGKKYRDDEKDDVSMRVIADHARAVTFLICDGVLPSNEGRGYVLRRIMRRAARHAKMLGIA
EPVLFRIVDAVNMMMGDAYPELLEREHYVKKVILAEEERFIETLDRGLAILNEETAALKAKGEKVISGEVIFKLYDTFGF
PVDLTADIVESEGFTLDEDGFALCMEKQRVKARENWKGSGEQGLADIYKELHGTGITTDFLGYSEQTAFSTISAIVKGGT
LVEEASAGDEIEIVTAATPFYGESGGQAGDTGTISTGSGHIEVTGSTRPFTDLIVHRGRVVSGAVRTGEAADLKITVANR
AATARNHTATHLLQAALREVLGDHVKQAGSLVTPDRLRFDFTHFTPMTQEEIRRVEILVNGHIMGNSTVESREMPAADAL
AAGATALFGEKYGDVVRVVKVGDVSSELCGGTHVHGAGEIGFFKIISEAGIAAGVRRIEAQTGSGALAVVHGMEDEQRGI
ATLLKAEGGTVLDKVEKLVAGQRELQKELETLQARLNASKSADLIQQVREQNGIKILSVKVDGDAKGLRELSDTLKQRIG
SGIIVLGTSDAARANLLVAVTADLSARWKAGDIIKAIAPIVGGNGGGKPELAQAGGSKPEHLAEALEAVYQIVG

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=968, Percent_Identity=38.3264462809917, Blast_Score=555, Evalue=1e-158,
Organism=Homo sapiens, GI38569417, Length=927, Percent_Identity=35.1672060409924, Blast_Score=441, Evalue=1e-123,
Organism=Escherichia coli, GI1789048, Length=864, Percent_Identity=51.1574074074074, Blast_Score=849, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=982, Percent_Identity=37.2708757637475, Blast_Score=531, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17536681, Length=750, Percent_Identity=34.6666666666667, Blast_Score=375, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324911, Length=757, Percent_Identity=40.6869220607662, Blast_Score=551, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24582809, Length=971, Percent_Identity=35.8393408856849, Blast_Score=532, Evalue=1e-151,
Organism=Drosophila melanogaster, GI45552267, Length=971, Percent_Identity=35.8393408856849, Blast_Score=532, Evalue=1e-151,
Organism=Drosophila melanogaster, GI24658214, Length=778, Percent_Identity=33.6760925449871, Blast_Score=372, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 95149; Mature: 95018

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA ; PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKEIRAQFFNFFQKKGHALVESSNLIPRNDPTLLFTNAGMNQFKDVFLGMEKRDYLRA
CCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHH
VSSQKCVRAGGKHNDLENVGRTARHHTFFEMLGNFSFGDYFKKEAIGFAWEFLTKELGLS
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
KDRLYVTVYTDDDEAADIWHNQEGVPRERIYRFGEKDNFWSMGDTGPCGPCTEIFWDNGP
CCEEEEEEEECCCCHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHEECCCC
GTGCGSPDCAVGCDCDRYMEIWNNVFMQFNRGKDGVLTPLPKPSVDTGMGLERISAVMQG
CCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VTSNYDTDLLQGIIRHIEQVSGKKYRDDEKDDVSMRVIADHARAVTFLICDGVLPSNEGR
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHEEHHHHHEEEEEEECCCCCCCCCC
GYVLRRIMRRAARHAKMLGIAEPVLFRIVDAVNMMMGDAYPELLEREHYVKKVILAEEER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
FIETLDRGLAILNEETAALKAKGEKVISGEVIFKLYDTFGFPVDLTADIVESEGFTLDED
HHHHHHHHHHHHCCCHHHHHHCCCEEECHHHHHHHHHHCCCCCCCHHHHHCCCCCEECCC
GFALCMEKQRVKARENWKGSGEQGLADIYKELHGTGITTDFLGYSEQTAFSTISAIVKGG
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHCCC
TLVEEASAGDEIEIVTAATPFYGESGGQAGDTGTISTGSGHIEVTGSTRPFTDLIVHRGR
CEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCHHHHHHHCCC
VVSGAVRTGEAADLKITVANRAATARNHTATHLLQAALREVLGDHVKQAGSLVTPDRLRF
EEECCCCCCCCCEEEEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
DFTHFTPMTQEEIRRVEILVNGHIMGNSTVESREMPAADALAAGATALFGEKYGDVVRVV
EEHHCCCCCHHHHHEEEEEEECEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEE
KVGDVSSELCGGTHVHGAGEIGFFKIISEAGIAAGVRRIEAQTGSGALAVVHGMEDEQRG
EECCCCHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHH
IATLLKAEGGTVLDKVEKLVAGQRELQKELETLQARLNASKSADLIQQVREQNGIKILSV
HHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEE
KVDGDAKGLRELSDTLKQRIGSGIIVLGTSDAARANLLVAVTADLSARWKAGDIIKAIAP
EECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHH
IVGGNGGGKPELAQAGGSKPEHLAEALEAVYQIVG
HHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TGKEIRAQFFNFFQKKGHALVESSNLIPRNDPTLLFTNAGMNQFKDVFLGMEKRDYLRA
CCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHH
VSSQKCVRAGGKHNDLENVGRTARHHTFFEMLGNFSFGDYFKKEAIGFAWEFLTKELGLS
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
KDRLYVTVYTDDDEAADIWHNQEGVPRERIYRFGEKDNFWSMGDTGPCGPCTEIFWDNGP
CCEEEEEEEECCCCHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHEECCCC
GTGCGSPDCAVGCDCDRYMEIWNNVFMQFNRGKDGVLTPLPKPSVDTGMGLERISAVMQG
CCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VTSNYDTDLLQGIIRHIEQVSGKKYRDDEKDDVSMRVIADHARAVTFLICDGVLPSNEGR
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHEEHHHHHEEEEEEECCCCCCCCCC
GYVLRRIMRRAARHAKMLGIAEPVLFRIVDAVNMMMGDAYPELLEREHYVKKVILAEEER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
FIETLDRGLAILNEETAALKAKGEKVISGEVIFKLYDTFGFPVDLTADIVESEGFTLDED
HHHHHHHHHHHHCCCHHHHHHCCCEEECHHHHHHHHHHCCCCCCCHHHHHCCCCCEECCC
GFALCMEKQRVKARENWKGSGEQGLADIYKELHGTGITTDFLGYSEQTAFSTISAIVKGG
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHCCC
TLVEEASAGDEIEIVTAATPFYGESGGQAGDTGTISTGSGHIEVTGSTRPFTDLIVHRGR
CEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCHHHHHHHCCC
VVSGAVRTGEAADLKITVANRAATARNHTATHLLQAALREVLGDHVKQAGSLVTPDRLRF
EEECCCCCCCCCEEEEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
DFTHFTPMTQEEIRRVEILVNGHIMGNSTVESREMPAADALAAGATALFGEKYGDVVRVV
EEHHCCCCCHHHHHEEEEEEECEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEE
KVGDVSSELCGGTHVHGAGEIGFFKIISEAGIAAGVRRIEAQTGSGALAVVHGMEDEQRG
EECCCCHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHH
IATLLKAEGGTVLDKVEKLVAGQRELQKELETLQARLNASKSADLIQQVREQNGIKILSV
HHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEE
KVDGDAKGLRELSDTLKQRIGSGIIVLGTSDAARANLLVAVTADLSARWKAGDIIKAIAP
EECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHH
IVGGNGGGKPELAQAGGSKPEHLAEALEAVYQIVG
HHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA