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The map label for this gene is ltaA [H]

Identifier: 197119780

GI number: 197119780

Start: 3934623

End: 3935642

Strand: Direct

Name: ltaA [H]

Synonym: Gbem_3418

Alternate gene names: 197119780

Gene position: 3934623-3935642 (Clockwise)

Preceding gene: 197119779

Following gene: 197119781

Centisome position: 85.25

GC content: 65.39

Gene sequence:

>1020_bases
ATGAAGACGGTAGATCTGAGAAGCGACACGGTGACATCGCCGTCGCAGGCGATGCGTCGGGAAATGGCGAATGCCCCGGT
AGGAGACGACGTCTACGGGGAGGACCCGACGGTAAACCGGCTGGAGTCCATGGCGGCAGAGTTGCTGGGGAAGGAGGCGG
CGCTCTTCGTCCCCACGGGGACCATGGGGAACCTGCTTGCCCTCTTATCGCACTGCGGACGCGGTGACGAGTACATCGCC
GGGCAGGAAGCGCACATCTACCGGTGGGAGGGAGGAGGAGGCGCCATCTTCGGCGGGATCCAGCCGCAGCCGATCGACTT
CGAAGAGGATGGGACGCTCGATCTCGAAAAGGTGCGACGCGTCGTGAAGCCCGCGGATTACCATCACCCCGTCACCAGGC
TCCTCTGCCTTGAGAACACTCAGGGGGGGAAAGTGTTGCCGCTCGACTATCTGGCAAAGGCTGCGGAGCTTGCCCAAGGC
CTCGGGCTTTCCCTGCATCTCGACGGGGCCCGAGTCTTCAATGCGGCCGTGTACCTGGGGGTCCCCGTCGCCACCATCGC
CGCCCATTTCGATTCGGCCTCGGTCTGCCTCTCCAAGGGACTTGGCGCCCCGGCAGGTACGGTACTTTGCGCCAGTAGGG
AGCTCATCGGCCGCGCGCGCCGCTGGCGCAAAGTAGCCGGCGGCGGCATGCGCCAGGCCGGCATTCTGGCCGCGGCCGGC
ATACATGCACTGGAGAAGAACCTGCAGCGACTGACCGAGGACCACGAGAACGCGGAACTCCTGGCCGCGGGCCTTGGGCA
CATAGAAGAACTCATGGTGAGCCAGGCGCGCACCAACATCCTCTTCGTCACCCCACCCGCTGGCAGCGCCGACCGCCTGC
GCCAGACCCTCGCCACCGAAGGGATACTCCTTGGCGGGGGGGACCAGATACGCCTGGTCACCCACCTTGACGTAACCGGC
GCGGACGTGGAACGCACCGTCGTCGCCTTCAAACGCTTCTTTGCAGCACGGGGGAACTGA

Upstream 100 bases:

>100_bases
CACCTTGCCGCCAACGGCGACCACGGGGTGAGCGTCACCGAAAAGGACAAGATCTGGGAAAACAAGTACCGCAGCAAAGA
TTAGCAAGGTAGGGGAACAG

Downstream 100 bases:

>100_bases
CGTGGCGGAAATAAGCTGGGACGATTTCGAAAAGGTGGAACTGCGGGTAGGGACCGTGCTGGAAGTGGAGGAATTTCCCG
AGGCGAGAAAGCCCGCCTAC

Product: L-threonine/L-allo-threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKTVDLRSDTVTSPSQAMRREMANAPVGDDVYGEDPTVNRLESMAAELLGKEAALFVPTGTMGNLLALLSHCGRGDEYIA
GQEAHIYRWEGGGGAIFGGIQPQPIDFEEDGTLDLEKVRRVVKPADYHHPVTRLLCLENTQGGKVLPLDYLAKAAELAQG
LGLSLHLDGARVFNAAVYLGVPVATIAAHFDSASVCLSKGLGAPAGTVLCASRELIGRARRWRKVAGGGMRQAGILAAAG
IHALEKNLQRLTEDHENAELLAAGLGHIEELMVSQARTNILFVTPPAGSADRLRQTLATEGILLGGGDQIRLVTHLDVTG
ADVERTVVAFKRFFAARGN

Sequences:

>Translated_339_residues
MKTVDLRSDTVTSPSQAMRREMANAPVGDDVYGEDPTVNRLESMAAELLGKEAALFVPTGTMGNLLALLSHCGRGDEYIA
GQEAHIYRWEGGGGAIFGGIQPQPIDFEEDGTLDLEKVRRVVKPADYHHPVTRLLCLENTQGGKVLPLDYLAKAAELAQG
LGLSLHLDGARVFNAAVYLGVPVATIAAHFDSASVCLSKGLGAPAGTVLCASRELIGRARRWRKVAGGGMRQAGILAAAG
IHALEKNLQRLTEDHENAELLAAGLGHIEELMVSQARTNILFVTPPAGSADRLRQTLATEGILLGGGDQIRLVTHLDVTG
ADVERTVVAFKRFFAARGN
>Mature_339_residues
MKTVDLRSDTVTSPSQAMRREMANAPVGDDVYGEDPTVNRLESMAAELLGKEAALFVPTGTMGNLLALLSHCGRGDEYIA
GQEAHIYRWEGGGGAIFGGIQPQPIDFEEDGTLDLEKVRRVVKPADYHHPVTRLLCLENTQGGKVLPLDYLAKAAELAQG
LGLSLHLDGARVFNAAVYLGVPVATIAAHFDSASVCLSKGLGAPAGTVLCASRELIGRARRWRKVAGGGMRQAGILAAAG
IHALEKNLQRLTEDHENAELLAAGLGHIEELMVSQARTNILFVTPPAGSADRLRQTLATEGILLGGGDQIRLVTHLDVTG
ADVERTVVAFKRFFAARGN

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=332, Percent_Identity=53.3132530120482, Blast_Score=345, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI193206522, Length=345, Percent_Identity=43.768115942029, Blast_Score=270, Evalue=9e-73,
Organism=Caenorhabditis elegans, GI193206520, Length=345, Percent_Identity=43.768115942029, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI193206524, Length=345, Percent_Identity=43.768115942029, Blast_Score=270, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6320789, Length=337, Percent_Identity=35.6083086053413, Blast_Score=178, Evalue=1e-45,
Organism=Drosophila melanogaster, GI21355439, Length=312, Percent_Identity=48.0769230769231, Blast_Score=272, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 36059; Mature: 36059

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVDLRSDTVTSPSQAMRREMANAPVGDDVYGEDPTVNRLESMAAELLGKEAALFVPTG
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
TMGNLLALLSHCGRGDEYIAGQEAHIYRWEGGGGAIFGGIQPQPIDFEEDGTLDLEKVRR
CHHHHHHHHHHCCCCCCEECCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHH
VVKPADYHHPVTRLLCLENTQGGKVLPLDYLAKAAELAQGLGLSLHLDGARVFNAAVYLG
HHCCCCCCCHHHHHEEEECCCCCEEECHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHC
VPVATIAAHFDSASVCLSKGLGAPAGTVLCASRELIGRARRWRKVAGGGMRQAGILAAAG
CHHHHHHHHCCCHHHHHHCCCCCCCHHHEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
IHALEKNLQRLTEDHENAELLAAGLGHIEELMVSQARTNILFVTPPAGSADRLRQTLATE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHC
GILLGGGDQIRLVTHLDVTGADVERTVVAFKRFFAARGN
CEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTVDLRSDTVTSPSQAMRREMANAPVGDDVYGEDPTVNRLESMAAELLGKEAALFVPTG
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
TMGNLLALLSHCGRGDEYIAGQEAHIYRWEGGGGAIFGGIQPQPIDFEEDGTLDLEKVRR
CHHHHHHHHHHCCCCCCEECCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHH
VVKPADYHHPVTRLLCLENTQGGKVLPLDYLAKAAELAQGLGLSLHLDGARVFNAAVYLG
HHCCCCCCCHHHHHEEEECCCCCEEECHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHC
VPVATIAAHFDSASVCLSKGLGAPAGTVLCASRELIGRARRWRKVAGGGMRQAGILAAAG
CHHHHHHHHCCCHHHHHHCCCCCCCHHHEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
IHALEKNLQRLTEDHENAELLAAGLGHIEELMVSQARTNILFVTPPAGSADRLRQTLATE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHC
GILLGGGDQIRLVTHLDVTGADVERTVVAFKRFFAARGN
CEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} 0.12 {DL-threo-phenylserine}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]