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The map label for this gene is ruvC

Identifier: 197119679

GI number: 197119679

Start: 3811094

End: 3811588

Strand: Direct

Name: ruvC

Synonym: Gbem_3314

Alternate gene names: 197119679

Gene position: 3811094-3811588 (Clockwise)

Preceding gene: 197119678

Following gene: 197119680

Centisome position: 82.58

GC content: 64.24

Gene sequence:

>495_bases
ATGATCATTCTCGGCATCGACCCAGGCTCCCGCAAGACCGGCTACGGCATCATCTCCAAACAGGGAAACCGCCTGATCCA
CGTCGACAACGGCGCCATCTTCACCCAGAGCGCCAAAGACTTCCCCGAGAGGCTGGAGAAGATCTTCACCGGGCTCTCCG
AGATCATCGCGCAGTACCGCCCCGAGGTGGTCGCGGTGGAGGACGTGTTCCTGGCGAAGAACGCCCAGAGCGCCTTGAAG
CTCGGGCAGGCGCGCGGCGCGGCCATCGTCGCCGCGGTGAACGTCGGCCTCCCGGTCCACGAGTACACGGCGATGCAGGT
GAAGCAGGCGGTGGTCGGCACCGGTCGCGCAGAAAAGGCGCAGGTGCAGCAGATGATCAAGGCGCTTTTGAACCTGCCGG
AGGTGGCGCAGGAAGACGCCTCCGACGCCCTGGCGGTCGCCATCTGCCACGCCCACTCCGCAGGCATCAATGCCCTTTTT
AAAAACGTCAGGTAG

Upstream 100 bases:

>100_bases
GAAGAGATGGAAAAGATGATGTAGCAAAGGCAGGGTCTTTTGTGGTACTAAGAGCGCGGGCTTGTCCCCGCGCTTTTTTT
TTAACTTTCAGGATTCAAAC

Downstream 100 bases:

>100_bases
CCACTAATGTTTCTTCACCGTCAATTGTCAATTGTCCATTGCCAATTGGTTCAGGAGAGTTTTTTATGATCGCGCTCCTT
ACCGGAAAGCTCGCCTACAA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MIILGIDPGSRKTGYGIISKQGNRLIHVDNGAIFTQSAKDFPERLEKIFTGLSEIIAQYRPEVVAVEDVFLAKNAQSALK
LGQARGAAIVAAVNVGLPVHEYTAMQVKQAVVGTGRAEKAQVQQMIKALLNLPEVAQEDASDALAVAICHAHSAGINALF
KNVR

Sequences:

>Translated_164_residues
MIILGIDPGSRKTGYGIISKQGNRLIHVDNGAIFTQSAKDFPERLEKIFTGLSEIIAQYRPEVVAVEDVFLAKNAQSALK
LGQARGAAIVAAVNVGLPVHEYTAMQVKQAVVGTGRAEKAQVQQMIKALLNLPEVAQEDASDALAVAICHAHSAGINALF
KNVR
>Mature_164_residues
MIILGIDPGSRKTGYGIISKQGNRLIHVDNGAIFTQSAKDFPERLEKIFTGLSEIIAQYRPEVVAVEDVFLAKNAQSALK
LGQARGAAIVAAVNVGLPVHEYTAMQVKQAVVGTGRAEKAQVQQMIKALLNLPEVAQEDASDALAVAICHAHSAGINALF
KNVR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=151, Percent_Identity=60.9271523178808, Blast_Score=164, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_GEOBB (B5EAH1)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002140106.1
- ProteinModelPortal:   B5EAH1
- SMR:   B5EAH1
- GeneID:   6780172
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_3314
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   PRK00039
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17485; Mature: 17485

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILGIDPGSRKTGYGIISKQGNRLIHVDNGAIFTQSAKDFPERLEKIFTGLSEIIAQYR
CEEEECCCCCCCCCCEEEECCCCEEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHHC
PEVVAVEDVFLAKNAQSALKLGQARGAAIVAAVNVGLPVHEYTAMQVKQAVVGTGRAEKA
CCEEEEHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHH
QVQQMIKALLNLPEVAQEDASDALAVAICHAHSAGINALFKNVR
HHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MIILGIDPGSRKTGYGIISKQGNRLIHVDNGAIFTQSAKDFPERLEKIFTGLSEIIAQYR
CEEEECCCCCCCCCCEEEECCCCEEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHHC
PEVVAVEDVFLAKNAQSALKLGQARGAAIVAAVNVGLPVHEYTAMQVKQAVVGTGRAEKA
CCEEEEHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHH
QVQQMIKALLNLPEVAQEDASDALAVAICHAHSAGINALFKNVR
HHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA