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The map label for this gene is metG [H]

Identifier: 197119575

GI number: 197119575

Start: 3697211

End: 3698740

Strand: Direct

Name: metG [H]

Synonym: Gbem_3207

Alternate gene names: 197119575

Gene position: 3697211-3698740 (Clockwise)

Preceding gene: 197119574

Following gene: 197119577

Centisome position: 80.11

GC content: 62.42

Gene sequence:

>1530_bases
ATGAAGCCCGCCTACTACGTCACCACCCCCATCTACTACGTAAACGACGTCCCGCACATAGGCCACGCATACACCACGCT
TGCCGCGGACGTACTGGCGCGCTACAAGCGGCTCAAGGGCTTCGACGTCTTCTTCCTGACCGGCACCGACGAGCACGGAC
AGAAGGTGGAGAAGGCTGCCATCGCCGCCGGCGAGACCCCGCTGGAGCTGGCCGACCGCGTCATGAAGCGTTTCCAGTCG
CTTTGGGAGAAGCTGGAGATCTCCAACACCGACTTCATCAGGACCACCCAGGAGCGCCACAAGAAAGGCGTTTCGGTGCT
CTTCGAGAGGGTGATGGAGAAAGGGGACATCTACCTCGGGGAGTATGAGGACTGGTACTGCACCCCGTGCGAAACCTTCT
GGACCGAGACGCAGTTGATCGACTACAAGTGCCCTGACTGCAACAGGCCTACCGAGAAGCTGAAGGAGGAGTCGTACTTC
TTCAGGATGAGCAAGTATCAGGACCAGCTTCTGGCCCACATAGAGGCGAACCCGGACTTCATCCAGCCCAAGAGCCGCAG
AAACGAGATCATCTCCTTCGTCAAGGAAGGGCTTCGCGACCTCTCCGTCTCCCGCACCACCTTCCAGTGGGGGATCCCGG
TCCCGGGCAACGACAAGCACGTGGTCTACGTCTGGTTCGATGCGCTGACGAACTACATCACCGCGCTCGGCTACCCCGAC
GAGCAAGGGAACTTCGGCAAGTACTGGCCCTGCGACGTGCACCTGATCGGCAAGGACATCCTGCGTTTCCACACCGTCTA
CTGGCCCACCTTCCTGATGGCGGCCGGGCTCCCGATCCCCAAGAAGGTGTTCGCCCACGGCTGGTGGACCGTCGAAGGGC
AGAAGATGAGCAAGAGCCTGCAGAACGTGGTCGAGCCCAACATGCTGGTCGACAAGTACGGGGTGGACGCGGTGCGCTAC
TTCCTGCTGCGCGAAGTCCCCTTCGGCCTGGACGGCGACTTCTCGCACCAGGCGCTGGTGCACAGGATCAACTCCGATCT
CGCCAACGACCTCGGTAACCTCCTGAACCGTTCCACCGCGATGCTGGGGAAATACTTCGGAGGCGTTTTGCAGGCCCCCG
GCGAGGAGACCGACCTGGACGCGGCCTACCGCGAGAAAACCGCGGCCATGATCGGCCAGGTGGACGGGTTCATCGAGGAC
CTAGCCTTCAGCCGCGCGCTGCAGGCGATCTGGGAGGTCATCTCCGCCGGCAACAAGTACATCGACGAGACCGCCCCCTG
GACCCTCGCCAAGGACCCGGAAAAAAGGGAGCGCCTGGCGACCGTCATGTACTACATGCTGGAGTCGCAGAGGGTGGTCT
ACTCGCTTCTCTCCGCCTTCATGCCCAAGACCTCCGAGAAGGGGCTTTGCTGCCTGGGTTGGCCGCAGGCGCCCGACGCC
GGCGACCTCGCCTGGGGTGGGCTCAAGCCCGGCACCCCCATAGCCAAGGCCGAGGCGCTCTTCCCGAGGATCGAGGAAAA
AGCGGAATAG

Upstream 100 bases:

>100_bases
AGCAGCAGCAGGGCGACAAGGGCAAAGGCCCGCAGGGCTCCGGCAAGCAGCGCCGCAACAGGCCCCAGGACGTTAAGGAA
AGAAAAAAGGAGAAACCACA

Downstream 100 bases:

>100_bases
CAAAACCAGTTCACGCAAAGACGCGAAGACGCAAAGAAAGACAAAAGGCGCTGCACAGGGTCCCCCTTGTGAAGCGCCTT
TTTTGCTTTTGCAGTTCTTT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MKPAYYVTTPIYYVNDVPHIGHAYTTLAADVLARYKRLKGFDVFFLTGTDEHGQKVEKAAIAAGETPLELADRVMKRFQS
LWEKLEISNTDFIRTTQERHKKGVSVLFERVMEKGDIYLGEYEDWYCTPCETFWTETQLIDYKCPDCNRPTEKLKEESYF
FRMSKYQDQLLAHIEANPDFIQPKSRRNEIISFVKEGLRDLSVSRTTFQWGIPVPGNDKHVVYVWFDALTNYITALGYPD
EQGNFGKYWPCDVHLIGKDILRFHTVYWPTFLMAAGLPIPKKVFAHGWWTVEGQKMSKSLQNVVEPNMLVDKYGVDAVRY
FLLREVPFGLDGDFSHQALVHRINSDLANDLGNLLNRSTAMLGKYFGGVLQAPGEETDLDAAYREKTAAMIGQVDGFIED
LAFSRALQAIWEVISAGNKYIDETAPWTLAKDPEKRERLATVMYYMLESQRVVYSLLSAFMPKTSEKGLCCLGWPQAPDA
GDLAWGGLKPGTPIAKAEALFPRIEEKAE

Sequences:

>Translated_509_residues
MKPAYYVTTPIYYVNDVPHIGHAYTTLAADVLARYKRLKGFDVFFLTGTDEHGQKVEKAAIAAGETPLELADRVMKRFQS
LWEKLEISNTDFIRTTQERHKKGVSVLFERVMEKGDIYLGEYEDWYCTPCETFWTETQLIDYKCPDCNRPTEKLKEESYF
FRMSKYQDQLLAHIEANPDFIQPKSRRNEIISFVKEGLRDLSVSRTTFQWGIPVPGNDKHVVYVWFDALTNYITALGYPD
EQGNFGKYWPCDVHLIGKDILRFHTVYWPTFLMAAGLPIPKKVFAHGWWTVEGQKMSKSLQNVVEPNMLVDKYGVDAVRY
FLLREVPFGLDGDFSHQALVHRINSDLANDLGNLLNRSTAMLGKYFGGVLQAPGEETDLDAAYREKTAAMIGQVDGFIED
LAFSRALQAIWEVISAGNKYIDETAPWTLAKDPEKRERLATVMYYMLESQRVVYSLLSAFMPKTSEKGLCCLGWPQAPDA
GDLAWGGLKPGTPIAKAEALFPRIEEKAE
>Mature_509_residues
MKPAYYVTTPIYYVNDVPHIGHAYTTLAADVLARYKRLKGFDVFFLTGTDEHGQKVEKAAIAAGETPLELADRVMKRFQS
LWEKLEISNTDFIRTTQERHKKGVSVLFERVMEKGDIYLGEYEDWYCTPCETFWTETQLIDYKCPDCNRPTEKLKEESYF
FRMSKYQDQLLAHIEANPDFIQPKSRRNEIISFVKEGLRDLSVSRTTFQWGIPVPGNDKHVVYVWFDALTNYITALGYPD
EQGNFGKYWPCDVHLIGKDILRFHTVYWPTFLMAAGLPIPKKVFAHGWWTVEGQKMSKSLQNVVEPNMLVDKYGVDAVRY
FLLREVPFGLDGDFSHQALVHRINSDLANDLGNLLNRSTAMLGKYFGGVLQAPGEETDLDAAYREKTAAMIGQVDGFIED
LAFSRALQAIWEVISAGNKYIDETAPWTLAKDPEKRERLATVMYYMLESQRVVYSLLSAFMPKTSEKGLCCLGWPQAPDA
GDLAWGGLKPGTPIAKAEALFPRIEEKAE

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=501, Percent_Identity=39.9201596806387, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI14043022, Length=505, Percent_Identity=26.7326732673267, Blast_Score=162, Evalue=9e-40,
Organism=Escherichia coli, GI1788432, Length=544, Percent_Identity=25.5514705882353, Blast_Score=195, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI212645096, Length=494, Percent_Identity=35.6275303643725, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI71992781, Length=384, Percent_Identity=32.2916666666667, Blast_Score=204, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17540794, Length=547, Percent_Identity=27.7879341864717, Blast_Score=174, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321610, Length=514, Percent_Identity=34.6303501945525, Blast_Score=295, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6321704, Length=555, Percent_Identity=26.3063063063063, Blast_Score=152, Evalue=9e-38,
Organism=Drosophila melanogaster, GI21358483, Length=494, Percent_Identity=37.246963562753, Blast_Score=324, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24655311, Length=554, Percent_Identity=27.4368231046931, Blast_Score=173, Evalue=3e-43,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58073; Mature: 58073

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPAYYVTTPIYYVNDVPHIGHAYTTLAADVLARYKRLKGFDVFFLTGTDEHGQKVEKAA
CCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
IAAGETPLELADRVMKRFQSLWEKLEISNTDFIRTTQERHKKGVSVLFERVMEKGDIYLG
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHCCEEEE
EYEDWYCTPCETFWTETQLIDYKCPDCNRPTEKLKEESYFFRMSKYQDQLLAHIEANPDF
CCCCCEECCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IQPKSRRNEIISFVKEGLRDLSVSRTTFQWGIPVPGNDKHVVYVWFDALTNYITALGYPD
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEHHHHHHHHHHCCCCC
EQGNFGKYWPCDVHLIGKDILRFHTVYWPTFLMAAGLPIPKKVFAHGWWTVEGQKMSKSL
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECHHHHHHHH
QNVVEPNMLVDKYGVDAVRYFLLREVPFGLDGDFSHQALVHRINSDLANDLGNLLNRSTA
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MLGKYFGGVLQAPGEETDLDAAYREKTAAMIGQVDGFIEDLAFSRALQAIWEVISAGNKY
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IDETAPWTLAKDPEKRERLATVMYYMLESQRVVYSLLSAFMPKTSEKGLCCLGWPQAPDA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCC
GDLAWGGLKPGTPIAKAEALFPRIEEKAE
CCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPAYYVTTPIYYVNDVPHIGHAYTTLAADVLARYKRLKGFDVFFLTGTDEHGQKVEKAA
CCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
IAAGETPLELADRVMKRFQSLWEKLEISNTDFIRTTQERHKKGVSVLFERVMEKGDIYLG
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHCCEEEE
EYEDWYCTPCETFWTETQLIDYKCPDCNRPTEKLKEESYFFRMSKYQDQLLAHIEANPDF
CCCCCEECCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IQPKSRRNEIISFVKEGLRDLSVSRTTFQWGIPVPGNDKHVVYVWFDALTNYITALGYPD
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEHHHHHHHHHHCCCCC
EQGNFGKYWPCDVHLIGKDILRFHTVYWPTFLMAAGLPIPKKVFAHGWWTVEGQKMSKSL
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECHHHHHHHH
QNVVEPNMLVDKYGVDAVRYFLLREVPFGLDGDFSHQALVHRINSDLANDLGNLLNRSTA
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MLGKYFGGVLQAPGEETDLDAAYREKTAAMIGQVDGFIEDLAFSRALQAIWEVISAGNKY
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IDETAPWTLAKDPEKRERLATVMYYMLESQRVVYSLLSAFMPKTSEKGLCCLGWPQAPDA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCC
GDLAWGGLKPGTPIAKAEALFPRIEEKAE
CCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]