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The map label for this gene is holB [H]

Identifier: 197119573

GI number: 197119573

Start: 3695169

End: 3696131

Strand: Direct

Name: holB [H]

Synonym: Gbem_3205

Alternate gene names: 197119573

Gene position: 3695169-3696131 (Clockwise)

Preceding gene: 197119572

Following gene: 197119574

Centisome position: 80.07

GC content: 65.21

Gene sequence:

>963_bases
ATGCCTTTTTCCGAGGTACTCGGCCAGGACTTGGCCGTATCCGTGCTCAGGCGCTCCATCGCCATGAACCGCGTTTCCCA
CGCCTACCTCTTCTCCGGAATCGAGGGGTGCGGGAAGAAAAAGACCGCCCTGGCCTTCGTGCAGGCGGTCTTCTGCGGCC
GCGACGAAGCCTGCGGCGTCTGCTCCTCCTGCCGCAAGATCGCCTCGGGGCAGCACCCGGACCTGCACATCCTGGAGCCC
GACGGCGCGTTCATCAAGATCGACCAGGTGCGTGAGCTGCAAAAAGAGCTATCCTACCGCCCCTTCGAGGCTCCCAAGAA
GGCGTGCATCATCGACGGGGCCGACAAGTTCAACCTCGCCTCAGGCAACGCGCTCCTGAAGACGCTGGAGGAACCGCCGG
GGAACGCCCTGATGATCCTGATCGCCACGGAGCGCTCGACTGTGCTGCAGACCATCCTGTCGCGCTGCCAGGCGCTCACC
TTTCAGCCGCTTCCGGCCGCGCTCATCGAGGAGCGGCTGGTGAGGGAGCAGTTCCCGGCGGAGGCGGCGCGGGTGGCCGC
CACCCTTTGCGGGGGGAGTCTCAGAAGGGGAATCGAGATCGCCACCGACGGCGTGCTCGAAGGTAGGGTGAGGTTCCTGG
AGCGGGTGCTCGCCCTGGAGCTTAAAGATATTGCCAAGCTTTTCGCCGCGGCCGAGGAGTTCGCGGCCGACAAAGAAGGG
CTCCCTGGGCTTTTGGAGCTGCTGCTCTCGTTTTTGAGGGACGTGCTGATCTACCGCTCAGCCCCGGAGGCCTTGGCCAA
CGCGGACCTGGCGCAACTGGTGGAGCGGGAGGCGTCCCGCTGCCGCGAGGGACGGGTGATGGAGCTGATCGAGCAGTTGG
CGGCAATGCGCCGGATGCTCGCCCGCAACGTGAACGCGCGGCTCGCCCTGGAAGTTTTCTTCATGAAGCTTGCGACGCGC
TAG

Upstream 100 bases:

>100_bases
AGACGTGCGGCAGACCGGGCTCCTCGTCGCAGAGGCCCTTCGCCAGAGGATGCAGAGCCTTGGCAAGGCCGGACTCGCAG
CAGTCAAGGCAGGGTGCTAG

Downstream 100 bases:

>100_bases
GGCCGCGAGAGCTTCAGCTCCCGGGCGGGATTAAGATAGCAGTAGAAGCGCCTGATGCCGGCTTGGCCGGCGGGCAATGC
AGCACCCAGACACAGCAAAC

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MPFSEVLGQDLAVSVLRRSIAMNRVSHAYLFSGIEGCGKKKTALAFVQAVFCGRDEACGVCSSCRKIASGQHPDLHILEP
DGAFIKIDQVRELQKELSYRPFEAPKKACIIDGADKFNLASGNALLKTLEEPPGNALMILIATERSTVLQTILSRCQALT
FQPLPAALIEERLVREQFPAEAARVAATLCGGSLRRGIEIATDGVLEGRVRFLERVLALELKDIAKLFAAAEEFAADKEG
LPGLLELLLSFLRDVLIYRSAPEALANADLAQLVEREASRCREGRVMELIEQLAAMRRMLARNVNARLALEVFFMKLATR

Sequences:

>Translated_320_residues
MPFSEVLGQDLAVSVLRRSIAMNRVSHAYLFSGIEGCGKKKTALAFVQAVFCGRDEACGVCSSCRKIASGQHPDLHILEP
DGAFIKIDQVRELQKELSYRPFEAPKKACIIDGADKFNLASGNALLKTLEEPPGNALMILIATERSTVLQTILSRCQALT
FQPLPAALIEERLVREQFPAEAARVAATLCGGSLRRGIEIATDGVLEGRVRFLERVLALELKDIAKLFAAAEEFAADKEG
LPGLLELLLSFLRDVLIYRSAPEALANADLAQLVEREASRCREGRVMELIEQLAAMRRMLARNVNARLALEVFFMKLATR
>Mature_319_residues
PFSEVLGQDLAVSVLRRSIAMNRVSHAYLFSGIEGCGKKKTALAFVQAVFCGRDEACGVCSSCRKIASGQHPDLHILEPD
GAFIKIDQVRELQKELSYRPFEAPKKACIIDGADKFNLASGNALLKTLEEPPGNALMILIATERSTVLQTILSRCQALTF
QPLPAALIEERLVREQFPAEAARVAATLCGGSLRRGIEIATDGVLEGRVRFLERVLALELKDIAKLFAAAEEFAADKEGL
PGLLELLLSFLRDVLIYRSAPEALANADLAQLVEREASRCREGRVMELIEQLAAMRRMLARNVNARLALEVFFMKLATR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=216, Percent_Identity=32.8703703703704, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787341, Length=289, Percent_Identity=25.9515570934256, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 35168; Mature: 35036

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFSEVLGQDLAVSVLRRSIAMNRVSHAYLFSGIEGCGKKKTALAFVQAVFCGRDEACGV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCH
CSSCRKIASGQHPDLHILEPDGAFIKIDQVRELQKELSYRPFEAPKKACIIDGADKFNLA
HHHHHHHHCCCCCCEEEECCCCCEEEHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCC
SGNALLKTLEEPPGNALMILIATERSTVLQTILSRCQALTFQPLPAALIEERLVREQFPA
CCHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCH
EAARVAATLCGGSLRRGIEIATDGVLEGRVRFLERVLALELKDIAKLFAAAEEFAADKEG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPGLLELLLSFLRDVLIYRSAPEALANADLAQLVEREASRCREGRVMELIEQLAAMRRML
CCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ARNVNARLALEVFFMKLATR
HHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PFSEVLGQDLAVSVLRRSIAMNRVSHAYLFSGIEGCGKKKTALAFVQAVFCGRDEACGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCH
CSSCRKIASGQHPDLHILEPDGAFIKIDQVRELQKELSYRPFEAPKKACIIDGADKFNLA
HHHHHHHHCCCCCCEEEECCCCCEEEHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCC
SGNALLKTLEEPPGNALMILIATERSTVLQTILSRCQALTFQPLPAALIEERLVREQFPA
CCHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCH
EAARVAATLCGGSLRRGIEIATDGVLEGRVRFLERVLALELKDIAKLFAAAEEFAADKEG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPGLLELLLSFLRDVLIYRSAPEALANADLAQLVEREASRCREGRVMELIEQLAAMRRML
CCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ARNVNARLALEVFFMKLATR
HHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]