Click here to switch to the map view.

The map label for this gene is tmk

Identifier: 197119572

GI number: 197119572

Start: 3694491

End: 3695168

Strand: Direct

Name: tmk

Synonym: Gbem_3204

Alternate gene names: 197119572

Gene position: 3694491-3695168 (Clockwise)

Preceding gene: 308535355

Following gene: 197119573

Centisome position: 80.05

GC content: 64.45

Gene sequence:

>678_bases
ATGGGCTTTTTCATTACATTTGAAGGTATAGAAGGGTGCGGCAAGACCACCCAGCTGAGGCTTTTGAAGGAGCGTCTGGA
AGCTGCGGGCGAGAAGGTGACCGTGACCCGGGAGCCTGGAGGGTGCCCGGTCGCCGACCAGATGCGCGCCATCCTGCTGG
ATGCCAAAAACAGCGCCATCACCCCTTTGGCTGAACTCCTTTTGTACGCGGCGGCCCGGGCGCAGCACGTGCAGGAAGTC
ATCGTCCCAGCGCTTGAGCGTGGCGAGACGGTCCTTTGCGACCGCTTTACCGACGCGACCGTCGCCTACCAGGGGCACGG
CAGGGGTCTCGACCTCACCGTCATCGAAGAACTCAACACCCTTGCCACCGGGAGGGTGCAGCCGGCGCTCACCGTGCTCA
TCGACTGCCCGGTGGAAGTGGGACTTTCCCGCGCCCTGGCCCGGATCGAGGCGACCAGCGGGGCGAAGGAAGAACGTTTC
GAGCGCGAGTCCCTGCTCTTCCACCAAAAGGTACGCAACGGCTACCTGACGCTGGCGGCCGCTTTCCCGGAACGCTTCGT
GGTTGTCGACGGCTCGGGAGACGTGCGGCAGACCGGGCTCCTCGTCGCAGAGGCCCTTCGCCAGAGGATGCAGAGCCTTG
GCAAGGCCGGACTCGCAGCAGTCAAGGCAGGGTGCTAG

Upstream 100 bases:

>100_bases
AAGGCACTAGCCACGCGGGTTTGCGGCCATTTAAGTCTTGCATATTCATCCCCCCTCCCATATAATTCAGTGATTTTATA
AGGATCATGAGACAAGAGCT

Downstream 100 bases:

>100_bases
ATGCCTTTTTCCGAGGTACTCGGCCAGGACTTGGCCGTATCCGTGCTCAGGCGCTCCATCGCCATGAACCGCGTTTCCCA
CGCCTACCTCTTCTCCGGAA

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MGFFITFEGIEGCGKTTQLRLLKERLEAAGEKVTVTREPGGCPVADQMRAILLDAKNSAITPLAELLLYAAARAQHVQEV
IVPALERGETVLCDRFTDATVAYQGHGRGLDLTVIEELNTLATGRVQPALTVLIDCPVEVGLSRALARIEATSGAKEERF
ERESLLFHQKVRNGYLTLAAAFPERFVVVDGSGDVRQTGLLVAEALRQRMQSLGKAGLAAVKAGC

Sequences:

>Translated_225_residues
MGFFITFEGIEGCGKTTQLRLLKERLEAAGEKVTVTREPGGCPVADQMRAILLDAKNSAITPLAELLLYAAARAQHVQEV
IVPALERGETVLCDRFTDATVAYQGHGRGLDLTVIEELNTLATGRVQPALTVLIDCPVEVGLSRALARIEATSGAKEERF
ERESLLFHQKVRNGYLTLAAAFPERFVVVDGSGDVRQTGLLVAEALRQRMQSLGKAGLAAVKAGC
>Mature_224_residues
GFFITFEGIEGCGKTTQLRLLKERLEAAGEKVTVTREPGGCPVADQMRAILLDAKNSAITPLAELLLYAAARAQHVQEVI
VPALERGETVLCDRFTDATVAYQGHGRGLDLTVIEELNTLATGRVQPALTVLIDCPVEVGLSRALARIEATSGAKEERFE
RESLLFHQKVRNGYLTLAAAFPERFVVVDGSGDVRQTGLLVAEALRQRMQSLGKAGLAAVKAGC

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=181, Percent_Identity=43.0939226519337, Blast_Score=126, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_GEOBB (B5E9M9)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139999.1
- ProteinModelPortal:   B5E9M9
- SMR:   B5E9M9
- GeneID:   6780062
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_3204
- HOGENOM:   HBG626009
- OMA:   LFITLEG
- ProtClustDB:   CLSK924585
- HAMAP:   MF_00165
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 24218; Mature: 24087

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFFITFEGIEGCGKTTQLRLLKERLEAAGEKVTVTREPGGCPVADQMRAILLDAKNSAI
CCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCC
TPLAELLLYAAARAQHVQEVIVPALERGETVLCDRFTDATVAYQGHGRGLDLTVIEELNT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHH
LATGRVQPALTVLIDCPVEVGLSRALARIEATSGAKEERFERESLLFHQKVRNGYLTLAA
HHCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
AFPERFVVVDGSGDVRQTGLLVAEALRQRMQSLGKAGLAAVKAGC
ECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GFFITFEGIEGCGKTTQLRLLKERLEAAGEKVTVTREPGGCPVADQMRAILLDAKNSAI
CEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCC
TPLAELLLYAAARAQHVQEVIVPALERGETVLCDRFTDATVAYQGHGRGLDLTVIEELNT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHH
LATGRVQPALTVLIDCPVEVGLSRALARIEATSGAKEERFERESLLFHQKVRNGYLTLAA
HHCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
AFPERFVVVDGSGDVRQTGLLVAEALRQRMQSLGKAGLAAVKAGC
ECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA