Click here to switch to the map view.

The map label for this gene is malQ-2 [H]

Identifier: 197117209

GI number: 197117209

Start: 967111

End: 968604

Strand: Reverse

Name: malQ-2 [H]

Synonym: Gbem_0817

Alternate gene names: 197117209

Gene position: 968604-967111 (Counterclockwise)

Preceding gene: 197117210

Following gene: 197117205

Centisome position: 20.99

GC content: 60.84

Gene sequence:

>1494_bases
ATGAAAAAGCGCGGTAGCGGGATTCTCTGTCACATCACTTCCCTCCCCTCCCCATTCGGCATAGGAGACTTTGGCGCCGG
CGCCTACGGGTTCGCCGATTTCCTGTGCGAGGCAAAACAGAGCTACTGGCAGATCCTGCCGCTCAACCCCACCAACAAGA
GGTATTTCGACTCCCCCTTCAGCTCCCTCTCCTCCTGCGCCGGGAATACCGCCCTAATCAGCCCTGAGTTGCTGGCGGCG
GAGGGGCTCCTGACGCAGCAGGAGGTGGAGGACCGTCCCCGCTTCCCGGAACACCGAGTGGATTACCAGGCCGCTTCGCG
TTTCAAGCGGCAGCTTTTCCAGCGGGCGCACACCCGCTTCCAGAAGGGAGGCGTACCGGAGGACTACGCCAGGTTCTGCC
GGGGCAACTCGATGTGGCTTGAGGACCATGCCCTCTTTACCGCGCTCAGCAATCATCTCGGCAATGCCCCGCTGACCGAA
TGGCCGCAGGAACTCATCAACCGCAACAGCGAGAAGGTGCAGCTGCTGCGTGAAGAGTTCTGGGACGAGGTAGAGCTGGA
GAAATTCCTGCAGTACCTCTTCTTCAAGCAGTGGCACCTGCTGAAGAACTACTGCAACGGCAAGGGGATCCGGATCATCG
GCGACTTCCCCATGTTCATGAACTACGATGCCGTGGACATCTGGACCAATCCGGAACTGTTCAAGGTCGATGAGCGGAAA
AAGCCTTACGTCCTCGCCGGGAGCCCCCCCGATGCCTTCAGCGCCGAAGGGCAGCTCTTCAACTGCGCGGTATACGACTG
GGACGGGCTGAAGCGTAACGGTTTCAACTGGTGGATCAGGCGCTTTCACTACCTGTTCAGGCAGTACGACTTTGTGAGGA
TCGACCATTTCCGCGGCCTGATCTCCTATTGGGAGGTCCCGGCAGGGGAGCAAAACGGGCTCAGGGGTAGCTGGCAGCAA
ACCCCGACGCAGGAATTCTTCAACGCAATGCTGCAGCACTATCCCCTCTTCCCGGTGATCGCCGAGGACCTGGGGACCAT
CGGCGCCGAGGTCAGGGAGACCATGGCACGGTACGCTATCCCCGGGATGAAGGTGCTCCTCTTGGGGTTCCAGAAGGAGG
CGCATGAAAAGAGCTACCTGCCGCACCTCCACCCCCGCAACAGCGTGGCCTACACCGGCACCCACGACACCGCCACCGTC
ACGGGGTGGTACCATGGCGCCGACGATAAGGAGCGGCAAGAACTCTTCCGCTATCTGGGGCGGCGGGCTGAGAACGGAGT
GAACTGGGAACTGATCCGGCTGGCGCTGATGTCGGTAGCGCATACGGCGGTGATTCAGATGCAGGACGTCTTAGGGGCGG
GCGAGGAATCGAGGATGAACAAACCAGGGAAAGCGGAAGGGAATTGGGAGTGGAGGGTCCCGCCAGGCGATCTTGGAGAG
GTCGCTTCCCGTCTCGCCGAGATGACGATCTGCTACGGACGGGCGCAGGATTGA

Upstream 100 bases:

>100_bases
GTGCTCTAGCCTCCGGTTAGGGAGCACCGTCAGTGACGCCGGAATCATCCACAAAGCCTCACCTGTTCCACGCAGGTGGG
GCTTTTCTACGGGAGGATTT

Downstream 100 bases:

>100_bases
TAATCGCCGGGCGCAAAAATGGGGCTTCGGGTTTTCCCGAAGCCCCATCGCTGTTCTTGCAGATGCTGGAGGCGCCGTTA
TTTCTCGACCCCGAGCTTCT

Product: 4-alpha-glucanotransferase

Products: NA

Alternate protein names: Amylomaltase; Disproportionating enzyme; D-enzyme [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MKKRGSGILCHITSLPSPFGIGDFGAGAYGFADFLCEAKQSYWQILPLNPTNKRYFDSPFSSLSSCAGNTALISPELLAA
EGLLTQQEVEDRPRFPEHRVDYQAASRFKRQLFQRAHTRFQKGGVPEDYARFCRGNSMWLEDHALFTALSNHLGNAPLTE
WPQELINRNSEKVQLLREEFWDEVELEKFLQYLFFKQWHLLKNYCNGKGIRIIGDFPMFMNYDAVDIWTNPELFKVDERK
KPYVLAGSPPDAFSAEGQLFNCAVYDWDGLKRNGFNWWIRRFHYLFRQYDFVRIDHFRGLISYWEVPAGEQNGLRGSWQQ
TPTQEFFNAMLQHYPLFPVIAEDLGTIGAEVRETMARYAIPGMKVLLLGFQKEAHEKSYLPHLHPRNSVAYTGTHDTATV
TGWYHGADDKERQELFRYLGRRAENGVNWELIRLALMSVAHTAVIQMQDVLGAGEESRMNKPGKAEGNWEWRVPPGDLGE
VASRLAEMTICYGRAQD

Sequences:

>Translated_497_residues
MKKRGSGILCHITSLPSPFGIGDFGAGAYGFADFLCEAKQSYWQILPLNPTNKRYFDSPFSSLSSCAGNTALISPELLAA
EGLLTQQEVEDRPRFPEHRVDYQAASRFKRQLFQRAHTRFQKGGVPEDYARFCRGNSMWLEDHALFTALSNHLGNAPLTE
WPQELINRNSEKVQLLREEFWDEVELEKFLQYLFFKQWHLLKNYCNGKGIRIIGDFPMFMNYDAVDIWTNPELFKVDERK
KPYVLAGSPPDAFSAEGQLFNCAVYDWDGLKRNGFNWWIRRFHYLFRQYDFVRIDHFRGLISYWEVPAGEQNGLRGSWQQ
TPTQEFFNAMLQHYPLFPVIAEDLGTIGAEVRETMARYAIPGMKVLLLGFQKEAHEKSYLPHLHPRNSVAYTGTHDTATV
TGWYHGADDKERQELFRYLGRRAENGVNWELIRLALMSVAHTAVIQMQDVLGAGEESRMNKPGKAEGNWEWRVPPGDLGE
VASRLAEMTICYGRAQD
>Mature_497_residues
MKKRGSGILCHITSLPSPFGIGDFGAGAYGFADFLCEAKQSYWQILPLNPTNKRYFDSPFSSLSSCAGNTALISPELLAA
EGLLTQQEVEDRPRFPEHRVDYQAASRFKRQLFQRAHTRFQKGGVPEDYARFCRGNSMWLEDHALFTALSNHLGNAPLTE
WPQELINRNSEKVQLLREEFWDEVELEKFLQYLFFKQWHLLKNYCNGKGIRIIGDFPMFMNYDAVDIWTNPELFKVDERK
KPYVLAGSPPDAFSAEGQLFNCAVYDWDGLKRNGFNWWIRRFHYLFRQYDFVRIDHFRGLISYWEVPAGEQNGLRGSWQQ
TPTQEFFNAMLQHYPLFPVIAEDLGTIGAEVRETMARYAIPGMKVLLLGFQKEAHEKSYLPHLHPRNSVAYTGTHDTATV
TGWYHGADDKERQELFRYLGRRAENGVNWELIRLALMSVAHTAVIQMQDVLGAGEESRMNKPGKAEGNWEWRVPPGDLGE
VASRLAEMTICYGRAQD

Specific function: Unknown

COG id: COG1640

COG function: function code G; 4-alpha-glucanotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the disproportionating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1789821, Length=459, Percent_Identity=24.1830065359477, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003385
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF02446 Glyco_hydro_77 [H]

EC number: =2.4.1.25 [H]

Molecular weight: Translated: 57252; Mature: 57252

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRGSGILCHITSLPSPFGIGDFGAGAYGFADFLCEAKQSYWQILPLNPTNKRYFDSPF
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHH
SSLSSCAGNTALISPELLAAEGLLTQQEVEDRPRFPEHRVDYQAASRFKRQLFQRAHTRF
HHHHHHCCCCEECCCHHHHHHCCCCHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHH
QKGGVPEDYARFCRGNSMWLEDHALFTALSNHLGNAPLTEWPQELINRNSEKVQLLREEF
HCCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHH
WDEVELEKFLQYLFFKQWHLLKNYCNGKGIRIIGDFPMFMNYDAVDIWTNPELFKVDERK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHCCCCEEEEECCCCEEEECCCC
KPYVLAGSPPDAFSAEGQLFNCAVYDWDGLKRNGFNWWIRRFHYLFRQYDFVRIDHFRGL
CCEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHH
ISYWEVPAGEQNGLRGSWQQTPTQEFFNAMLQHYPLFPVIAEDLGTIGAEVRETMARYAI
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
PGMKVLLLGFQKEAHEKSYLPHLHPRNSVAYTGTHDTATVTGWYHGADDKERQELFRYLG
CCCEEEEEHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHH
RRAENGVNWELIRLALMSVAHTAVIQMQDVLGAGEESRMNKPGKAEGNWEWRVPPGDLGE
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEECCCCCHHH
VASRLAEMTICYGRAQD
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKRGSGILCHITSLPSPFGIGDFGAGAYGFADFLCEAKQSYWQILPLNPTNKRYFDSPF
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHH
SSLSSCAGNTALISPELLAAEGLLTQQEVEDRPRFPEHRVDYQAASRFKRQLFQRAHTRF
HHHHHHCCCCEECCCHHHHHHCCCCHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHH
QKGGVPEDYARFCRGNSMWLEDHALFTALSNHLGNAPLTEWPQELINRNSEKVQLLREEF
HCCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHH
WDEVELEKFLQYLFFKQWHLLKNYCNGKGIRIIGDFPMFMNYDAVDIWTNPELFKVDERK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHCCCCEEEEECCCCEEEECCCC
KPYVLAGSPPDAFSAEGQLFNCAVYDWDGLKRNGFNWWIRRFHYLFRQYDFVRIDHFRGL
CCEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHH
ISYWEVPAGEQNGLRGSWQQTPTQEFFNAMLQHYPLFPVIAEDLGTIGAEVRETMARYAI
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
PGMKVLLLGFQKEAHEKSYLPHLHPRNSVAYTGTHDTATVTGWYHGADDKERQELFRYLG
CCCEEEEEHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHH
RRAENGVNWELIRLALMSVAHTAVIQMQDVLGAGEESRMNKPGKAEGNWEWRVPPGDLGE
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEECCCCCHHH
VASRLAEMTICYGRAQD
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]