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The map label for this gene is polA [H]

Identifier: 197117169

GI number: 197117169

Start: 910260

End: 911684

Strand: Reverse

Name: polA [H]

Synonym: Gbem_0774

Alternate gene names: 197117169

Gene position: 911684-910260 (Counterclockwise)

Preceding gene: 197117170

Following gene: 197117168

Centisome position: 19.75

GC content: 60.56

Gene sequence:

>1425_bases
ATGGAAATTTCGCCGCGGCTCTATCTGCTGGATGGATCCAGTTACATCTATCGCGCCTATTACGGCTTCCGGGATATCGC
CACCCCCGGCGGAATGCCTGCCAATGCCATTTTCGGTTTCACGAAGATGCTCCTCGACCTGCTGCAGGAGCATCGTCCCG
AATATTTCGCCGTCGTCTTCGACCCTCCCCGTGAAAATACCTTTCGCCGCGAAATGTACCCGGACTATAAGGCGCAGCGC
GACGCAATGCCCGAGGACCTGGTTTCCCAACTCCCGTACCTACGGAAGATACTGCAGACTCTCAACATCCCCATCCTGGA
AGCCTCTCGCTTTGAGGCCGACGACGTCATTGCCACCCTCGCCGCTAGGTTCGCCGCGGAAGGAGCGGATGTCACCGTAG
TCACCGGCGACAAGGATTTACTGCAGATCGTCACCGACCGGATTGCCCTTTTGGACACGATGAAGGGGAAGCGTTCGGGG
CCGCAGCAGGTTGTCGAGCGCTTCGGCGTCCCTCCTGAATTGGTGGCCGATGTCCTTGGGCTTGCCGGCGACAGCGGAGA
CAATATCCCGGGAGTCCCGGGGATAGGCGAGAAGATAGCGGCAAAGCTGGTACAGCAGTTCGGATCCCTTGAAAAAGTCC
TGGAGTGGCGAAGCCTGGTGAACGGGAAGAGCCGCAGGGAGAATCTGAAAACCCATGCGGAACAGGCGATTCTCTCCAAA
GCGCTTGCCACCGTTCGCTACGACGCTCCCGTTGATGTATCCCTGGCAGAGTTGCAGAGCCGGCCGGCGAGCGTCCAGGA
ACTGGTTTCCCTGCTGCGCGAGTTGGGTTTTGCCGCCCTGGAGGTTGCCTTCACCCCGCCGCCGCCCGGCATTGTGGAGA
TCTACAGCGACGGTTCGGGAAGGGACTCCGGGCCGGGGGGATATGGCGTGATCCTGCGCTATGGGGAGCACGAAAAGGAG
CTGAGTGGTTTCGAGCCCAGCTCAACCTCACAGCGTATGGAGCTGATTGCAGCGATCAGGGGACTGGAGGCGCTCAACGC
TCCGAGCAGGGTGCGCGTTTTCAGCGATTCGCAGTACCTGGTACGGGGGATGAGCGAATGGCTAGCAGGCTGGATACGTG
CCGGACGCCTTGAGACACCCGACGCTCTGAAGAACCAGGATCTCTGGCGTCAACTCGCCGACCTGTCAGCCAGGCACAAG
GTAGAGTGGGAATGGGTGGCAGGACATGCGGGGCATCCGTTCAACGAGCGTTGCGACAAACTGGCTAAGCGAGCGGCAGA
AGAGGGCGCTCGCGCCGTGATCCCCGTCACCCCCGAGATTCCGACCATCCCGCCTTCGCCCCCACCCGTTCAGCCGATGC
CGCTCCTTTGCACAGAGGAAAGAACATTTGATGAAGAGGCAGACGGCCAGTTCCGCCTGTGCTGA

Upstream 100 bases:

>100_bases
GCGAGCCTGAGGCACCGGTGACAGGCACAGGTACGGATATGGATTGTAAAGCTCCCCTTGAGATAGTAGTATCCGGCGGG
AATTCTTTGTGGGAGAGGGT

Downstream 100 bases:

>100_bases
TGCTGAAATATAAGGAGAACAAACGAATACCAATGGTTCGTCGTTTCCTTCTCCAACTGCGCCCTCTTCTTGAACCACGA
CGGCGCCCTGAGCTTTGGTC

Product: ribonuclease H

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MEISPRLYLLDGSSYIYRAYYGFRDIATPGGMPANAIFGFTKMLLDLLQEHRPEYFAVVFDPPRENTFRREMYPDYKAQR
DAMPEDLVSQLPYLRKILQTLNIPILEASRFEADDVIATLAARFAAEGADVTVVTGDKDLLQIVTDRIALLDTMKGKRSG
PQQVVERFGVPPELVADVLGLAGDSGDNIPGVPGIGEKIAAKLVQQFGSLEKVLEWRSLVNGKSRRENLKTHAEQAILSK
ALATVRYDAPVDVSLAELQSRPASVQELVSLLRELGFAALEVAFTPPPPGIVEIYSDGSGRDSGPGGYGVILRYGEHEKE
LSGFEPSSTSQRMELIAAIRGLEALNAPSRVRVFSDSQYLVRGMSEWLAGWIRAGRLETPDALKNQDLWRQLADLSARHK
VEWEWVAGHAGHPFNERCDKLAKRAAEEGARAVIPVTPEIPTIPPSPPPVQPMPLLCTEERTFDEEADGQFRLC

Sequences:

>Translated_474_residues
MEISPRLYLLDGSSYIYRAYYGFRDIATPGGMPANAIFGFTKMLLDLLQEHRPEYFAVVFDPPRENTFRREMYPDYKAQR
DAMPEDLVSQLPYLRKILQTLNIPILEASRFEADDVIATLAARFAAEGADVTVVTGDKDLLQIVTDRIALLDTMKGKRSG
PQQVVERFGVPPELVADVLGLAGDSGDNIPGVPGIGEKIAAKLVQQFGSLEKVLEWRSLVNGKSRRENLKTHAEQAILSK
ALATVRYDAPVDVSLAELQSRPASVQELVSLLRELGFAALEVAFTPPPPGIVEIYSDGSGRDSGPGGYGVILRYGEHEKE
LSGFEPSSTSQRMELIAAIRGLEALNAPSRVRVFSDSQYLVRGMSEWLAGWIRAGRLETPDALKNQDLWRQLADLSARHK
VEWEWVAGHAGHPFNERCDKLAKRAAEEGARAVIPVTPEIPTIPPSPPPVQPMPLLCTEERTFDEEADGQFRLC
>Mature_474_residues
MEISPRLYLLDGSSYIYRAYYGFRDIATPGGMPANAIFGFTKMLLDLLQEHRPEYFAVVFDPPRENTFRREMYPDYKAQR
DAMPEDLVSQLPYLRKILQTLNIPILEASRFEADDVIATLAARFAAEGADVTVVTGDKDLLQIVTDRIALLDTMKGKRSG
PQQVVERFGVPPELVADVLGLAGDSGDNIPGVPGIGEKIAAKLVQQFGSLEKVLEWRSLVNGKSRRENLKTHAEQAILSK
ALATVRYDAPVDVSLAELQSRPASVQELVSLLRELGFAALEVAFTPPPPGIVEIYSDGSGRDSGPGGYGVILRYGEHEKE
LSGFEPSSTSQRMELIAAIRGLEALNAPSRVRVFSDSQYLVRGMSEWLAGWIRAGRLETPDALKNQDLWRQLADLSARHK
VEWEWVAGHAGHPFNERCDKLAKRAAEEGARAVIPVTPEIPTIPPSPPPVQPMPLLCTEERTFDEEADGQFRLC

Specific function: In addition to polymerase activity, this DNA polymerase 5' to 3' exonuclease activity [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=277, Percent_Identity=40.4332129963899, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1786408, Length=137, Percent_Identity=48.1751824817518, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI226510970, Length=214, Percent_Identity=35.0467289719626, Blast_Score=125, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=31.2056737588652, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 52422; Mature: 52422

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEISPRLYLLDGSSYIYRAYYGFRDIATPGGMPANAIFGFTKMLLDLLQEHRPEYFAVVF
CCCCCEEEEEECCCEEEHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
DPPRENTFRREMYPDYKAQRDAMPEDLVSQLPYLRKILQTLNIPILEASRFEADDVIATL
CCCCCCCHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHH
AARFAAEGADVTVVTGDKDLLQIVTDRIALLDTMKGKRSGPQQVVERFGVPPELVADVLG
HHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHH
LAGDSGDNIPGVPGIGEKIAAKLVQQFGSLEKVLEWRSLVNGKSRRENLKTHAEQAILSK
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ALATVRYDAPVDVSLAELQSRPASVQELVSLLRELGFAALEVAFTPPPPGIVEIYSDGSG
HHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCHHEEEEEECCCCCCEEEEECCCCC
RDSGPGGYGVILRYGEHEKELSGFEPSSTSQRMELIAAIRGLEALNAPSRVRVFSDSQYL
CCCCCCCEEEEEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHH
VRGMSEWLAGWIRAGRLETPDALKNQDLWRQLADLSARHKVEWEWVAGHAGHPFNERCDK
HHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
LAKRAAEEGARAVIPVTPEIPTIPPSPPPVQPMPLLCTEERTFDEEADGQFRLC
HHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEECC
>Mature Secondary Structure
MEISPRLYLLDGSSYIYRAYYGFRDIATPGGMPANAIFGFTKMLLDLLQEHRPEYFAVVF
CCCCCEEEEEECCCEEEHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
DPPRENTFRREMYPDYKAQRDAMPEDLVSQLPYLRKILQTLNIPILEASRFEADDVIATL
CCCCCCCHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHH
AARFAAEGADVTVVTGDKDLLQIVTDRIALLDTMKGKRSGPQQVVERFGVPPELVADVLG
HHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHH
LAGDSGDNIPGVPGIGEKIAAKLVQQFGSLEKVLEWRSLVNGKSRRENLKTHAEQAILSK
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ALATVRYDAPVDVSLAELQSRPASVQELVSLLRELGFAALEVAFTPPPPGIVEIYSDGSG
HHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCHHEEEEEECCCCCCEEEEECCCCC
RDSGPGGYGVILRYGEHEKELSGFEPSSTSQRMELIAAIRGLEALNAPSRVRVFSDSQYL
CCCCCCCEEEEEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHH
VRGMSEWLAGWIRAGRLETPDALKNQDLWRQLADLSARHKVEWEWVAGHAGHPFNERCDK
HHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
LAKRAAEEGARAVIPVTPEIPTIPPSPPPVQPMPLLCTEERTFDEEADGQFRLC
HHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA