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The map label for this gene is gph [C]

Identifier: 197117161

GI number: 197117161

Start: 902744

End: 903412

Strand: Direct

Name: gph [C]

Synonym: Gbem_0766

Alternate gene names: 197117161

Gene position: 902744-903412 (Clockwise)

Preceding gene: 197117160

Following gene: 197117162

Centisome position: 19.56

GC content: 59.04

Gene sequence:

>669_bases
ATGGCTCCTACACGGCTGCTCATTTTCGACCTGGACGGGACGCTGATCGATTCGCTCCCCGACCTGACCGACGCCACCAA
CCTGATCCGCAGCAACTACGGCCTCCCCGAAATAGGGATTCCCGAGGTCCGCAAGTTGGTAGGGCAGGGGGCGCGGAACC
TGGTAGAGCGGGCGCTCCCCGGGGCAACGGCGGCGCAGGTGGACCAGGCGCTGGGCGTATTTCTCGATTACAACCTGGCC
CACATAGCCGACAAGACCCGTCCCTATCCGGGCGTCAGCGAGACCCTTAAGGAGCTTAGGAACTTCGACATCCCCATGGT
GGTCCTCTCCAACAAGAACGTCGCCCTCTGCAGGGAAGTCCTCGCCAAGCTGGCTATCGGGGACGCCTTCGCCGAGGTAT
TCGGGGCCGACTCCTTCCCCTACAGGAAGCCCTCCCCCGAGCCTGTGCTGGCCGTCCTGAAACAATATAAGATCGAAGCC
GCTGAATGCGTCATGGTGGGGGACAGTATCAACGACATCGCAGCAGGATTAGGGGCAGGCGTTTTCACCGTCGGCTGCAG
TTACGGTTATGGCGAGGTGAGTGAGCTTGCAGGAGCCAACTATCAGGTATCAGATTTTCAGTCATTGCTGAATTTGCCGT
TTTTTAACAGGAAAAGCAGTGAGCAATGA

Upstream 100 bases:

>100_bases
GCGGCTCTTTCGTATCTTGAAGACTGGTTCCGCAGGTGGGGACTGGCTCCGCCAGGTGCCTGTCCCCCTTTCGCCGTCCC
TCTTTACTTGGAGAAAGAAC

Downstream 100 bases:

>100_bases
AAAAAAGCTAAAGTTATTTTCTTAGCTGTCGAAACAGAACTAAACCTTATTCCGTACCTAACTTATCGGGGGCAAGAATC
AGATGAAAATCGAAGAACTC

Product: HAD superfamily hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MAPTRLLIFDLDGTLIDSLPDLTDATNLIRSNYGLPEIGIPEVRKLVGQGARNLVERALPGATAAQVDQALGVFLDYNLA
HIADKTRPYPGVSETLKELRNFDIPMVVLSNKNVALCREVLAKLAIGDAFAEVFGADSFPYRKPSPEPVLAVLKQYKIEA
AECVMVGDSINDIAAGLGAGVFTVGCSYGYGEVSELAGANYQVSDFQSLLNLPFFNRKSSEQ

Sequences:

>Translated_222_residues
MAPTRLLIFDLDGTLIDSLPDLTDATNLIRSNYGLPEIGIPEVRKLVGQGARNLVERALPGATAAQVDQALGVFLDYNLA
HIADKTRPYPGVSETLKELRNFDIPMVVLSNKNVALCREVLAKLAIGDAFAEVFGADSFPYRKPSPEPVLAVLKQYKIEA
AECVMVGDSINDIAAGLGAGVFTVGCSYGYGEVSELAGANYQVSDFQSLLNLPFFNRKSSEQ
>Mature_221_residues
APTRLLIFDLDGTLIDSLPDLTDATNLIRSNYGLPEIGIPEVRKLVGQGARNLVERALPGATAAQVDQALGVFLDYNLAH
IADKTRPYPGVSETLKELRNFDIPMVVLSNKNVALCREVLAKLAIGDAFAEVFGADSFPYRKPSPEPVLAVLKQYKIEAA
ECVMVGDSINDIAAGLGAGVFTVGCSYGYGEVSELAGANYQVSDFQSLLNLPFFNRKSSEQ

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=243, Percent_Identity=32.0987654320988, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23913; Mature: 23781

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPTRLLIFDLDGTLIDSLPDLTDATNLIRSNYGLPEIGIPEVRKLVGQGARNLVERALP
CCCCEEEEEECCCHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
GATAAQVDQALGVFLDYNLAHIADKTRPYPGVSETLKELRNFDIPMVVLSNKNVALCREV
CCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHH
LAKLAIGDAFAEVFGADSFPYRKPSPEPVLAVLKQYKIEAAECVMVGDSINDIAAGLGAG
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHEEEECCCHHHHHHHHCCH
VFTVGCSYGYGEVSELAGANYQVSDFQSLLNLPFFNRKSSEQ
HHHHCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
APTRLLIFDLDGTLIDSLPDLTDATNLIRSNYGLPEIGIPEVRKLVGQGARNLVERALP
CCCEEEEEECCCHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
GATAAQVDQALGVFLDYNLAHIADKTRPYPGVSETLKELRNFDIPMVVLSNKNVALCREV
CCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHH
LAKLAIGDAFAEVFGADSFPYRKPSPEPVLAVLKQYKIEAAECVMVGDSINDIAAGLGAG
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHEEEECCCHHHHHHHHCCH
VFTVGCSYGYGEVSELAGANYQVSDFQSLLNLPFFNRKSSEQ
HHHHCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA